Next Generation Sequencing Interest Group - A complete strategy for characterizing on- and off-target CRISPR-Cas9 editing via target enrichment, NGS analysis

Genome editing with the CRISPR/Cas9 system is moving towards therapeutic applications, which drives an increased need for in-depth characterization of both on- and off-target genome editing events. IDT will present a workflow and useful guidelines for prediction, validation, quantification, and analysis of on- and off-target editing events using rhAmpSeqTM, a multiplexed, amplicon-based enrichment method. Data using this technology to compare on- and off-target editing events associated with different CRISPR/Cas9 nucleases will be discussed.