Logo for University of Iowa Health Care This logo represents the University of Iowa Health Care

Next Generation Sequencing Interest Group: 10x Genomics sample preparation for Chromium single cell gene expression, ATAC, and multiome solutions

First, we will provide an overview of 10x Genomics Chromium and Visium solutions. Next, we will cover general sample preparation guidelines for Chromium single cell solutions, including important concepts when isolating cells or nuclei from mouse embryonic and adult brains. Finally, we will review how to isolate nuclei for single cell gene expression, ATAC, and multiome ATAC + gene expression solutions.

Individuals with disabilities are encouraged to attend all University of Iowa–sponsored events. If you are a person with a disability who requires a reasonable accommodation in order to participate in this program, please contact Iowa Institute of Human Genetics in advance at (319)335-3688 or iihg@uiowa.edu