Analyzing – Cytek Aurora and Becton Dickinson LSR II

Analyzing involves using a flow cytometer to interrogate cells stained with fluorescent markers and classifying them into groups. Cells pass through the instrument once and go straight to a waste container. There is no cell recovery.

Cell Sorting –  Cytek Aurora CS, Becton Dickinson FACS Fusion

Cell sorting involves using a flow cytometer with cell sorting capability to interrogate cells stained with fluorescent markers, classifying them into groups, and then physically separate the groups into test tubes or multi-well plates. The fraction of the starting population defined by the gating strategy is recovered, the remainder of cells go to a waste container.