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Flow Cytometry Education

Video

Web-based flow cytometry training
Animated tutorials presented by Becton Dickinson covering the following flow cytometry subjects. (requires a browser that supports Flash)

Introduction to Flow Cytometry (12 minutes)
Animated tutorial presented by Invitrogen (requires a browser that supports Flash)

Data Analysis for Flow Cytometry  (17 minutes)
Animated tutorial presented by Invitrogen (requires a browser that supports Flash)

Multi-color Flow Cytometry  (1 hour, 8 minutes)
Covers all aspects of multi-color experiments from fluorochrome chemistry to controls, divided into chapters,  presented by BioLegend

Work-Flow

     Contact Facility staff for login and password

Cytek Full Spectrum Panel Design Series (Spectrolearn)


Print Media


FlowJo v10


The Impact of Adjusting PMT Voltages on Spillover and Compensation

The slides at the following link explain why adjusting PMT voltages to lower compensation values does not improve immunofluorescent experiment results.

Impact of PMT Voltages on Spillover and Compensation


Biexponential Scaling

Biexponential scaling is an option that allows log-scaled data that is on or below the baseline to be displayed on scale.

Examples of Biexponential Scaling

Discussion of biexponential scaling option for log-scaled data.


Statistics

Calculation for Comparing Populations (Staining Index)

This calculation can be used for normalizing the relationship between positive and negative populations to compare treated and untreated samples.

SI = (MFIpos – MFIneg ) / (2 x SDneg

SI = staining index
MFI = median, geometric-mean, or mean fluorescence intensity
SD = standard deviation.

Staining Index Explained (links to Biolegend)

Note: Population average fluorescence intensity values can be skewed depending on the population distribution. Median fluorescence intensity is less skewed than geometric-mean fluorescence intensity and geometric-mean fluorescence intensity is less skewed than mean fluorescence intensity for log-normal populations (populations that look gaussian when plotted on a log scale).

 

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