NextGen Sequencing Interest Group

The Next Generation Sequencing (NGS) Interest Group meeting usually occur every 3rd Wednesday from 2:00 to 3:00 pm at Seebohm Conference Room (283 EMRB). The NGS Interest Group Meeting is open to everyone. Investigators that are currently performing or are planning to start studies using the Next Gen Sequencing platforms are encouraged to attend. Please contact Kevin Knudtson, PhD (kevin-knudtson@uiowa.edu) if you have questions about the NGS Interest Group. 

Upcoming Meetings

May 15, 2019

Speaker: Colin Kenny PhD, Post-Doctoral Researcher, Anatomy and Cell Biology, University of Iowa.

Targeted in-situ genome-wide mapping of histone modifications and transcription factors using CUT&RUN analysis.

Cleavage Under Targets and Release Using Nuclease Sequencing (CUT&RUN-sequencing) is a powerful technique used to profile histone modifications and transcription factors genome wide. CUT&RUN incorporates a novel hybrid Protein A/G-Micrococcal Nuclease (pA/G-MNase) to target antibody-protein complexes within unfixed permeabilized cells. Once activated, pA/G-MNase cleaves the adjacent DNA resulting in the targeted release of protein-DNA complexes into the supernatant for paired-end sequencing. CUT&RUN requires low cell numbers (1-100+ cells) and outperforms ChIP-Seq protocols in resolution, signal-to-noise ratio and depth of sequencing required.