Genome Sequencing Fees

Library Preparation Fees (as of 5/1/2018)



DNA-Seq (e.g., whole genome/De Novo) using KAPA Hyper Prep $110 $130
RNA-Seq (Expression transcript profiling) using Illumina TruSeq stranded mRNA (oligo-dT) $152 $180
RNA-Seq using Illumina TruSeq stranded total RNA w/ribozero $224 $252
RNA-Seq using Illumina TruSeqstranded total RNA w/ribozero gold $224 $252
smRNA-Seq (e.g., microRNA profiling) using Illumina TruSeq Small RNA $236 $266
Exome Capture using Agilent SureSelect (v5 or v6+UTR) $350   $472 
Please inquire for other custom target capture project pricing    


Genome Sequencer Fees (as of 5/1/2018)


Illumina HiSeq4000 (per lane)


Off Campus

50 SR (50 bp single end read) $1,422 $1,472
75 PE (75 bp paired end read) $1,798 $1,948
150 PE (150 bp paired end read) $2,320 $2,620

Illumina MiSeq

50 cycle (50 SR) $1,004 $1,054
150 cycle (150 SR or 75 PE) $1,184 $1,284
300 cycle (300 SR or 150 PE) $1,468 $1,568
500 cycle (500 SR or 250 PE) $1,688 $1,888
600 cycle (300 PE) $2,119 $2,419

Please Note: The cost of a Genome or Next Generation Sequencing (NGS) project typically includes 2 fees: 1) Library Preparation fee (assessed on a per sample basis), and 2) Genome Sequencer Fee (assessed on a per flow cell lane basis).  For many applications, more than one sample can be run in a single lane.  The number of samples run per lane will depend on the depth of sequence coverage per sample required.  A NGS project cost can be calculated using the formula below.

NGS Cost = (#samples x library prep fee) + (#flow cell lanes x genome sequencer fee)

Costs for Commonly Requested Applications*

Gene Expression Profiling using mRNA-Seq:

$291/sample (At least 20 million paired-end reads) Data generated using 75 PE paired sequencing chemistry run on an Illumina HiSeq 4000. Library samples prepared using the Illumina mRNA-Seq stranded kit.

Whole Genome Sequencing (WGS):

$1,607/sample (~20X coverage of a human genome). Sequence generated using 150 PE chemistry run on an Illumina HiSeq4000.

$2,356/sample (26-30X coverage of a human genome).  Sequence generated using 150 PE chemistry run on an Illumina HiSeq4000.

Whole Exome Sequencing (WES):

$631/sample (Human, Agilent SureSelect v5 51 Mb Exome, >3.8 Gb output, >50X average on-Target coverage)

$912/sample (Human, Agilent SureSelect v5 51 Mb Exome, >7.6 Gb output, >100X average on-target coverage)

*The costs for the commonly requested applications are based on the specific run parameters noted below each application.  A change in the run parameters would result in a change in the cost.


Contact Information

116 EMRB
Phone: (319) 335-6736 
Fax: (319) 335-6737

For information about downstream bioinformatics support, contact the IIHG Bioinformatics Division (319) 335-6717.