Lee-Ann H. Allen, PhD

Portrait
Professor of Internal Medicine - Infectious Diseases
Professor of Microbiology and Immunology
Associate Director - Center for Immunology and Immune-based Diseases

Contact Information

Office
505 EMRB
431 Newton Rd
Iowa City, IA 52242
319-335-4258

Education

BS, Biochemistry, University of California-Riverside
MS, Biochemistry, University of California-Riverside
PhD, Biochemistry, University of Wisconsin-Madison
Postdoctoral Fellow, Laboratory of Cellular Physiology and Immunology, and Laboratory of Signal Transduction, The Rockefeller University

Education/Training Program Affiliations

Biomedical Science Program, Department of Microbiology Graduate Program, Interdisciplinary Graduate Program in Immunology, Interdisciplinary Graduate Program in Molecular Medicine, Interdisciplinary Graduate Program in Translational Biomedicine, Medical Scientist Training Program

Center, Program and Institute Affiliations

Center for Immunology and Immune-based Diseases, Inflammation Program

Research Summary

The Allen laboratory studies Helicobacter pylori and Francisella tularensis with a focus on pathogen manipulation of macrophage and neutrophil function and dysregulation of the inflammatory response. Our approach is multi-faceted and addresses fundamental questions in cellular microbiology which lie at the interface of bacterial pathogenesis, phagocyte cell biology and innate immunity.

Helicobacter pylori colonizes the gastric mucosa of 50% of all humans elicits a chronic neutrophil-dominant inflammatory response that can progress to peptic ulceration or gastric cancer. H. pylori thrives in this neutrophil-rich environment, but how this is achieved is poorly defined. At the same time, understanding of neutrophil function has been revolutionized in the past few years by the discovery of their phenotypic plasticity and capacity to undergo subtype differentiation in different tissue microenvironments in vivo. We recently discovered that H. pylori induces N1-like subtype differentiation of human neutrophils, which is defined by profound nuclear hypersegmentation, changes in surface markers, and a proinflammatory and cytotoxic phenotype. These studies are significant as they are the first evidence that neutrophil subtype differentiation can be induced directly by bacterial infection in vitro. Current projects focus on elucidation of the underlying molecular mechanisms, including identification of new virulence factors and studies of the functional consequences with respect to neutrophil manipulation of NK cell, T-cell and epithelial cell function. Experimental approaches include analysis of intracellular signaling, cytokine production, cell viability, and dual RNA-Seq for simultaneous analysis of bacterial and host gene expression during infection.

Francisella tularensis is a facultative intracellular pathogen and the causative agent of the zoonotic disease tularemia. Inhalation of as few as 10 bacteria into the lungs can be fatal. In keeping with this, F. tularensis infects several cell types, including macrophages and neutrophils, and escapes the phagosome to replicate in the cytosol. In prior studies we identified receptors used by this organism to infect host cells, discovered multiple mechanisms used to disrupt neutrophil and macrophage defense mechanisms, and characterized novel phenotypes of mutants lacking the pathogenicity island gene pdpC or with defects in capsule and O-antigen production. Neutrophils are very short-lived, and current studies are based on our discovery that F. tularensis markedly prolongs neutrophil lifespan via inhibition of major apoptosis pathways. On the bacterial side, we have identified F. tularensis lipoproteins as important, but other virulence factors await discovery and characterization. On the host side we have begun new studies based on the premise that neutrophil longevity is coupled to profound F. tularensis-driven metabolic reprogramming that promotes host cell survival while also providing nutrients to enhance bacterial growth.

Publications

Kinkead, L. C., Whitmore, L. C., McCracken, J. M., Fletcher, J. R., Ketelsen, B. B., Kaufman, J. W., Jones, B. D., Weiss, D. S., Barker, J. H. & Allen, L. H. (2018). Bacterial lipoproteins and other factors released by Francisella tularensis modulate human neutrophil lifespan: Effects of a TLR1 SNP on apoptosis inhibition. Cellular microbiology, 20(2). PMID: 29063667.

Schulert, G. S., Hollenback, C. & Allen, L. H. (2018). Global disruption of phagosome maturationin Francisella tularensis-infected machrophages via aberrant protain kniase C-alpha targeting. Cell Micro.

Kinkead, L. C., Fayram, D. C. & Allen, L. H. (2017). Francisella novicida inhibits spontaneous apoptosis and extends human neutrophil lifespan. Journal of leukocyte biology, 102(3), 815-828. PMID: 28550119.

Allen, L. H. (2017). NR4A Proteins and Neutrophil Lifespan. Blood, 130(8), 958-959. PMID: 28838938.

Whitmore, L. C., Weems, M. N. & Allen, L. H. (2017). Cutting Edge: Helicobacter pylori Induces Nuclear Hypersegmentation and Subtype Differentiation of Human Neutrophils In Vitro. Journal of immunology (Baltimore, Md. : 1950), 198(5), 1793-1797. PMID: 28148734.

Kinkead, L. C., Allen, L. A. (2016). Multifaceted effects of Francisella tularensis on human neutrophil function and lifespan. Immunological reviews, 273(1), 266-81. PMID: 27558340.

McCracken, J. M., Kinkead, L. C., McCaffrey, R. L. & Allen, L. A. (2016). Francisella tularensis Modulates a Distinct Subset of Regulatory Factors and Sustains Mitochondrial Integrity to Impair Human Neutrophil Apoptosis. Journal of innate immunity, 8(3), 299-313. PMID: 26906922.

Leung, A., Gregory, N. S., Allen, L. A. & Sluka, K. A. (2016). Regular physical activity prevents chronic pain by altering resident muscle macrophage phenotype and increasing interleukin-10 in mice. Pain, 157(1), 70-9. PMID: 26230740.

Allen, L. H. (2016). Microbicidal Mechanisms. In R. A. Bradshaw , P. D. Stahl (Eds.) Encyclopedia of Cell Biology. pp. 766-775. Waltham, MA: Academic Press.

Rasmussen, J. A., Fletcher, J. R., Long, M. E., Allen, L. A. & Jones, B. D. (2015). Characterization of Francisella tularensis Schu S4 mutants identified from a transposon library screened for O-antigen and capsule deficiencies. Frontiers in microbiology, 6, 338. PMID: 25999917.

Dassie, J. P., Hernandez, L. I., Thomas, G. S., Long, M. E., Rockey, W. M., Howell, C. A., Chen, Y., Hernandez, F. J., Liu, X. Y., Wilson, M. E., Allen, L. A., Vaena, D. A., Meyerholz, D. K. & Giangrande, P. H. (2014). Targeted inhibition of prostate cancer metastases with an RNA aptamer to prostate-specific membrane antigen. Molecular therapy : the journal of the American Society of Gene Therapy, 22(11), 1910-22. PMID: 24954476.

Bandyopadhyay, S., Long, M. E. & Allen, L. A. (2014). Differential expression of microRNAs in Francisella tularensis-infected human macrophages: miR-155-dependent downregulation of MyD88 inhibits the inflammatory response. PloS one, 9(10), e109525. PMID: 25295729.

McCracken, J. M., Allen, L. A. (2014). Regulation of human neutrophil apoptosis and lifespan in health and disease. Journal of cell death, 7, 15-23. PMID: 25278783.

Allen, L. A. (2014). Immunofluorescence and confocal microscopy of neutrophils. Methods in molecular biology (Clifton, N.J.), 1124, 251-68. PMID: 24504957.

Allen, L. A. (2013). Neutrophils: potential therapeutic targets in tularemia?. Frontiers in cellular and infection microbiology, 3, 109. PMID: 24409419.

Faron, M., Fletcher, J. R., Rasmussen, J. A., Long, M. E., Allen, L. A. & Jones, B. D. (2013). The Francisella tularensis migR, trmE, and cphA genes contribute to F. tularensis pathogenicity island gene regulation and intracellular growth by modulation of the stress alarmone ppGpp. Infection and immunity, 81(8), 2800-11. PMID: 23716606.

Allen, L. H. (2013). Editorial: Leukocytes in tularemia--so many cells, so little time. Journal of leukocyte biology, 93(5), 641-4. PMID: 23633479.

Long, M. E., Lindemann, S. R., Rasmussen, J. A., Jones, B. D. & Allen, L. A. (2013). Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infection and immunity, 81(3), 850-61. PMID: 23275090.

Schwartz, J. T., Bandyopadhyay, S., Kobayashi, S. D., McCracken, J., Whitney, A. R., Deleo, F. R. & Allen, L. A. (2013). Francisella tularensis alters human neutrophil gene expression: insights into the molecular basis of delayed neutrophil apoptosis. Journal of innate immunity, 5(2), 124-36. PMID: 22986450.

Casbon, A. J., Long, M. E., Dunn, K. W., Allen, L. H. & Dinauer, M. C. (2012). Effects of IFNgamma on intracellular trafficking and activity of macrophage NADPH oxidase flavocytochrome b558. Journal of leukocyte biology, 92(4), 869-82. PMID: 22822009.

Schwartz, J. T., Barker, J. H., Long, M. E., Kaufman, J., McCracken, J. & Allen, L. A. (2012). Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum. Journal of immunology (Baltimore, Md. : 1950), 189(6), 3064-77. PMID: 22888138.

Schwartz, J. T., Barker, J. H., Kaufman, J., Fayram, D. C., McCracken, J. M. & Allen, L. A. (2012). Francisella tularensis inhibits the intrinsic and extrinsic pathways to delay constitutive apoptosis and prolong human neutrophil lifespan. Journal of immunology (Baltimore, Md. : 1950), 188(7), 3351-63. PMID: 22357630.

Mandal, P., Allen, L. A. (2012). Aberrant phagosome maturation and degranulation in Francisella tularensis-infected neutrophils and HL-60 cells. CCOM Research Week.

Long, M. E., Lindemann, S. R., Rasmussen, J. A., Jones, B. D. & Allen, L. A. (2012). Disruption of Francisella tularensis Schu S4 ilgI, iglJ and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice, and reveals a unique phenotype for pdpC. CCOM Research Week.

Long, M. E., Lindemann, S. R., Rasmussen, J. A., Jones, B. D. & Allen, L. A. (2012). Disruption of Francisella tularensis Schu S4 ilgI, iglJ and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice, and reveals a unique phenotype for pdpC. International Tularemia Meeting.

Schwartz, J. T., Barker, J. H., Kaufman, J., Fayram, D. C., McCracken, J. M. & Allen, L. A. (2012). Francisella tularensis inhibits the intrinsic and extrinsic pathways to delay constitutive apoptosis and prolong human neutrophil lifespan. International Tularemia Meeting.

Kinkead, L., McCracken, J., Schwartz, J. & Allen, L. A. (2012). Insights into the mechanisms of neutrophil apoptosis inhibition by Francisella tularensis. International Tularemia Meeting.

Cambronero, J. G., Allen, L. H., Cathcart, M. K., Justement, L. B., Kovacs, E. J., McLeish, K. R. & Nauseef, W. M. (2012). Writing a first grant proposal. Nat. Immunol, 13, 105-108.

Lindemann, S. R., Peng, K., Long, M. E., Hunt, J. R., Apicella, M. A., Monack, D. M., Allen, L. A. & Jones, B. D. (2011). Francisella tularensis Schu S4 O-antigen and capsule biosynthesis gene mutants induce early cell death in human macrophages. Infection and immunity, 79(2), 581-94. PMID: 21078861.

Rodriguez, N. E., Guar, U., Allen, L. A. & Wilson, M. E. (2011). Macrophage cholesterol mediates the entry, phagosome maturation, and intracellular survival of Leishmania infantum chagasi in a stage-specific and virulence-dependent manner. UI Research Week.

Rodri­guez, N. E., Gaur Dixit, U., Allen, L. H. & Wilson, M. E. (2011). Stage-specific pathways of Leishmania infantum chagasi entry and phagosome maturation in macrophages. PloS one, 6(4), e19000. PMID: 21552562.

McCaffrey, R. L., Schwartz, J. T., Lindemann, S. R., Moreland, J. G., Buchan, B. W., Jones, B. D. & Allen, L. H. (2010). Multiple mechanisms of NADPH oxidase inhibition by type A and type B Francisella tularensis. J Leukoc Biol, 88(4), 791-805. PMID: 20610796.

Lindemann, S. R., Apicella, M. A., Jones, B. D. & Allen, L. A. (2010). A gene locus in Francisella tularensis subspecies tularensis essential for capsule and LPS O-antigen biosynthesis, macrophage viability and virulence. Society for Leukocyte Biology.

Rodriguez, N. E., Allen, L. A. & Wilson, M. E. (2010). Fate of Leishamania chagasi in macrophages. Society for Leukocyte Biology.

Buchan, B. W., McCaffrey, R. L., Lindemann, S. R., Allen, L. A. & Jones, B. D. (2009). Identification of migR, a regulatory element of the Francisella tularensis live vaccine strain iglABCD virulence operon required for normal replication and trafficking in macrophages. Infection and immunity, 77(6), 2517-29. PMID: 19349423.

Schulert, G. S., McCaffrey, R. L., Buchan, B. W., Lindemann, S. R., Hollenback, C., Jones, B. D. & Allen, L. H. (2009). Francisella tularensis genes required for inhibition of the neutrophil respiratory burst and intramacrophage growth identified by random transposon mutagenesis of strain LVS. Infect Immun, 77(4), 1324-36. PMID: 19204089.

Casbon, A. J., Allen, L. A., Dunn, K. W. & Dinauer, M. C. (2009). Macrophage NADPH oxidase flavocytochrome B localizes to the plasma membrane and Rab11-positive recycling endosomes. Journal of immunology (Baltimore, Md. : 1950), 182(4), 2325-39. PMID: 19201887.

Schwartz, J. T., Allen, L. A. (2009). Effects of Francisella tularensis on human neutrophil lifespan. International Conference on Tularemia.

Schwartz, J. T., Allen, L. A. (2009). Francisella tularensis delays human neutrophil apoptosis to prolong cell lifespan. Phagocytes Gordon Conference.

Long, M. E., Allen, L. A. (2009). Francisella tularensis resists elimination by INFg-activated human macrophages. Phagocytes Gordon Conference.

Buchan, B. W., McCaffrey, R. L., Lindemann, S. R., Allen, L. A. & Jones, B. D. (2009). Identification and Phenotypic Characterization of a New Regulator of Virulence Gene Expression in Francisella tularensis. ASM General Meeting.

Rodriguez, N. E., Guar, U., Allen, L. A. & Wilson, M. E. (2009). Macrophage cholesterol mediates the entry, phagosome maturation and intracellular survival of Leishmania chagasi parasites in a stage-specific and virulence-dependent manner. American Society of Tropical Medicine and Hygiene.

McCaffrey, R. L., Allen, L. A. (2009). Multiple mechanisms of NADPH oxidase inhibition by Francisella tularensis. Phagocytes Gordon Conference.

Barker, J. H., McCaffrey, R. L., Baman, N. K., Allen, L. A., Weiss, J. P. & Nauseef, W. M. (2009). The role of complement opsonization in interactions between F. tularensis subsp. novicida and human neutrophils. Microbes and infection / Institut Pasteur, 11(8-9), 762-9. PMID: 19409509.

Allen, L. H., Schulert, G. S. (2009). Francisella tularensis. In U. E. Schaible , A. Haas (Eds.) Intracellular Niches of Microbes - A Pathogen's Guide through the Host Cell. pp. 415-430. Wiley-VCH.

Casbon, A. J., Allen, L. H. (2008). Distinct localization of the NADPH oxidase flavocytochrome in resting and IFN?-activated macrophages. (Vols. Supplement). pp. 58. Society of Leukocyte Meeting, Denver, CO. Published in J Leukoc Biol.

Allen, L. H., Schulert, G. S. (2008). Francisella tularensis inhibits protein kinase C-alpha to prevents phagosome maturation in macrophages. (Vols. Supplement). pp. 38. Society for Leukocyte Biology Meeting, Denver, CO. Published in J Leukoc Biol.

McCaffrey, R. L., Allen, L. H. (2008). Francisella tularensis inhibits neutrophil NADPH oxidase activation by two distinct mechanisms. (Vols. Supplement). pp. 21. Society for Leukocyte Biology Meeting, denver, CO. Published in J Leukoc Biol.

Barker, J. H., Baman, N. K. & Allen, L. H. (2008). Francisella tularensis LVS escapes from the phagosome of human monocyte-derived dendritic cells. (Vols. Supplement). pp. 24. Society for Leukocyte Biology Meeting, Denver, Co., Published in J Leukoc Biol.

Allen, L. H. (2008). Phagocytosis of Helicobacter pylori. Methods Mol Biol, 431, 147-157.

Allen, L. A. (2008). Rate and extent of Helicobacter pylori phagocytosis. Methods in molecular biology (Clifton, N.J.), 431, 147-57. PMID: 18287754.

Nakano, Y., Banfi, B., Jesaitis, A. J., Dinauer, M. C., Allen, L. A. & Nauseef, W. M. (2007). Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. The Biochemical journal, 403(1), 97-108. PMID: 17140397.

Allen, L. H. (2007). Phagocytosis and persistence of Helicobacter pylori. Cellular microbiology, 9(4), 817-28. PMID: 17346311.

Allen, L. H. (2007). Braking neutrophils with PTEN. Blood, 109, 3620-3621.

Allen, L. H. (2007). Immunofluorescence and confocal microscopy of neutrophils. Methods Mol Biol, 412, 273-287.

Allen, L. H., McCaffrey, R. L. (2007). To activate or not to activate: distinct strategies used by Helicobacter pylori and Francisella tularensis to modulate the NADPH oxidase and survive in human neutrophils. Immunol. Rev, 219, 103-117.

McCaffrey, R. L., Allen, L. A. (2006). Pivotal Advance: Francisella tularensis LVS evades killing by human neutrophils via inhibition of the respiratory burst and phagosome escape. Journal of leukocyte biology, 80(6), 1224-30. PMID: 16908516.

Schulert, G. S., Allen, L. A. (2006). Differential infection of mononuclear phagocytes by Francisella tularensis: role of the macrophage mannose receptor. Journal of leukocyte biology, 80(3), 563-71. PMID: 16816147.

Schwartz, J. T., Allen, L. A. (2006). Role of urease in megasome formation and Helicobacter pylori survival in macrophages. Journal of leukocyte biology, 79(6), 1214-25. PMID: 16543403.

McLendon, M. K., Apicella, M. A. & Allen, L. H. (2006). Francisella tularensis: taxonomy, genetics and immunopathogenesis of a potential agent of biowarfare. Annu. Rev. Microbiol, 60, 167-185. PMID: 16704343.

Allen, L. H. (2006). Disruption of Phagocyte Function by Helicobacter pylori. In J. D. Ernst , O. Stendahl (Eds.) Phagocytosis of Bacteria and Bacterial Pathogenicity. pp. 91-134. Cambridge University Press.

Allen, L. H. (2006). Interview with Dr. Lee-Ann Allen regarding Pivotal Advance: Francisella tularensis LVS evades killing by human neutrophils via inhibition of the respiratory burst and phagosome escape. Interview by Helene F. Rosenberg. (Vols. 80). pp. 1222-1223. Journal of Leukocyte Biology.

Allen, L. A., Allgood, J. A., Han, X. & Wittine, L. M. (2005). Phosphoinositide3-kinase regulates actin polymerization during delayed phagocytosis of Helicobacter pylori. Journal of leukocyte biology, 78(1), 220-30. PMID: 15809290.

Allen, L. A., Beecher, B. R., Lynch, J. T., Rohner, O. V. & Wittine, L. M. (2005). Helicobacter pylori disrupts NADPH oxidase targeting in human neutrophils to induce extracellular superoxide release. Journal of immunology (Baltimore, Md. : 1950), 174(6), 3658-67. PMID: 15749904.

Allen, L. H. (2003). Mechanisms of pathogenesis: evasion of killing by polymorphonuclear leukocytes. Microbes and infection / Institut Pasteur, 5(14), 1329-35. PMID: 14613776.

Allen, L. A., Yang, C. & Pessin, J. E. (2002). Rate and extent of phagocytosis in macrophages lacking vamp3. Journal of leukocyte biology, 72(1), 217-21. PMID: 12101283.

Allen, L. H., Allgood, J. A. (2002). Atypical protein kinase C-zeta is essential for delayed phagocytosis of Helicobacter pylori. Curr Biol, 12, 1762-1766. PMID: 12401171.

Allen, L. H. (2001). The role of the neutrophil and phagocytosis in infection caused by Helicobacter pylori. Current opinion in infectious diseases, 14(3), 273-7. PMID: 11964843.

Gallois, A., Klein, J. R., Allen, L. A., Jones, B. D. & Nauseef, W. M. (2001). Salmonella pathogenicity island 2-encoded type III secretion system mediates exclusion of NADPH oxidase assembly from the phagosomal membrane. Journal of immunology (Baltimore, Md. : 1950), 166(9), 5741-8. PMID: 11313417.

Yang, C., Mora, S., Ryder, J. W., Coker, K. J., Hansen, P., Allen, L. A. & Pessin, J. E. (2001). VAMP3 null mice display normal constitutive, insulin- and exercise-regulated vesicle trafficking. Molecular and cellular biology, 21(5), 1573-80. PMID: 11238894.

Myung, P. S., Clements, J. L., White, D. W., Malik, Z. A., Cowdery, J. S., Allen, L. H., Harty, J. T. & Kusner, D. J. (2000). In vitro and in vivo macrophage function can occur independently of SLP-76. International immunology, 12(6), 887-97. PMID: 10837416.

Allen, L. H. (2000). Modulating phagocyte activation: the pros and cons of Helicobacter pylori virulence factors. The Journal of experimental medicine, 191(9), 1451-4. PMID: 10790419.

Allen, L. A., Schlesinger, L. S. & Kang, B. (2000). Virulent strains of Helicobacter pylori demonstrate delayed phagocytosis and stimulate homotypic phagosome fusion in macrophages. The Journal of experimental medicine, 191(1), 115-28. PMID: 10620610.

DeLeo, F. R., Allen, L. A., Apicella, M. & Nauseef, W. M. (1999). NADPH oxidase activation and assembly during phagocytosis. Journal of immunology (Baltimore, Md. : 1950), 163(12), 6732-40. PMID: 10586071.

Allen, L. A., DeLeo, F. R., Gallois, A., Toyoshima, S., Suzuki, K. & Nauseef, W. M. (1999). Transient association of the nicotinamide adenine dinucleotide phosphate oxidase subunits p47phox and p67phox with phagosomes in neutrophils from patients with X-linked chronic granulomatous disease. Blood, 93(10), 3521-30. PMID: 10233905.

Allen, L. H. (1999). Intracellular niches for extracellular bacteria: lessons from Helicobacter pylori. J. Leukoc. Biol, 66, 753-756. PMID: 10577505.

Underhill, D. M., Chen, J., Allen, L. H. & Aderem, A. (1999). MacMARCKS is not essential for phagocytosis in macrophages. J Biol Chem, 273, 33619-33623. PMID: 9837946.

Myat, M. M., Anderson, S., Allen, L. H. & Aderem, A. (1997). MARCKS regulates membrane ruffling and cell spreading. Current biology : CB, 7(8), 611-4. PMID: 9259558.

Allen, L. A., Aderem, A. (1996). Molecular definition of distinct cytoskeletal structures involved in complement- and Fc receptor-mediated phagocytosis in macrophages. The Journal of experimental medicine, 184(2), 627-37. PMID: 8760816.

Seykora, J. T., Myat, M. M., Allen, L. A., Ravetch, J. V. & Aderem, A. (1996). Molecular determinants of the myristoyl-electrostatic switch of MARCKS. The Journal of biological chemistry, 271(31), 18797-802. PMID: 8702537.

Allen, L. H., Aderem, A. (1996). Mechanisms of phagocytosis. Curr Opin Immunol, 8(1), 36-40. PMID: 8729444.

Allen, L. H., Aderem, A. (1995). A role for MARCKS, the alpha isozyme of protein kinase C and myosin I in zymosan phagocytosis by macrophages. The Journal of experimental medicine, 182(3), 829-40. PMID: 7650489.

Allen, L. A., Aderem, A. (1995). Protein kinase C regulates MARCKS cycling between the plasma membrane and lysosomes in fibroblasts. The EMBO journal, 14(6), 1109-21. PMID: 7720702.

Allen, L. A., Hope, L., Raetz, C. R. & Thieringer, R. (1994). Genetic evidence supporting the role of peroxisome assembly factor (PAF)-1 in peroxisome biogenesis. Polymerase chain reaction detection of a missense mutation in PAF-1 of Chinese hamster ovary cells. The Journal of biological chemistry, 269(16), 11734-42. PMID: 7512952.

Allen, L. A., Raetz, C. R. (1992). Partial phenotypic suppression of a peroxisome-deficient animal cell mutant treated with aminoglycoside G418. The Journal of biological chemistry, 267(19), 13191-9. PMID: 1618823.

Aderem, A., Allen, L. H. (1992). Myristoylated Protein Kinase C Substrates and Intracellular Signaling Pathways in Macrophages. In R. van Furth (Eds.) Mononuclear Phagocytes: Biology of Monocytes and Macrophages. pp. 451-462. Kluwer Academic Press.

Morand, O. H., Allen, L. A., Zoeller, R. A. & Raetz, C. R. (1990). A rapid selection for animal cell mutants with defective peroxisomes. Biochimica et biophysica acta, 1034(2), 132-41. PMID: 2354189.

Zoeller, R. A., Allen, L. A., Santos, M. J., Lazarow, P. B., Hashimoto, T., Tartakoff, A. M. & Raetz, C. R. (1989). Chinese hamster ovary cell mutants defective in peroxisome biogenesis. Comparison to Zellweger syndrome. The Journal of biological chemistry, 264(36), 21872-8. PMID: 2689450.

Allen, L. A., Morand, O. H. & Raetz, C. R. (1989). Cytoplasmic requirement for peroxisome biogenesis in Chinese hamster ovary cells. Proceedings of the National Academy of Sciences of the United States of America, 86(18), 7012-6. PMID: 2780559.

Bers, D. M., Allen, L. A. & Kim, Y. (1986). Calcium binding to cardiac sarcolemmal vesicles: potential role as a modifier of contraction. The American journal of physiology, 251(6 Pt 1), C861-71. PMID: 3024498.

Karu, A. E., Allen, L. A. (1982). Interaction of hybridoma antibodies with normal and mutant forms of Escherichia coli recA protein. Biochimie, 64(8-9), 723-9. PMID: 6182924.