Steven Clegg, PhD

Portrait
Professor of Microbiology and Immunology

Contact Information

Office: 3-334 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
319-335-7778

Lab: 3-315 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
319-335-7787

Education

BSc, Microbiology, University of Dundee
PhD, Pathogenic Bacteriology, University of Dundee

Postdoctoral Fellow, E. coli ETEC virulence, University of Texas Health Science Center at Houston

Education/Training Program Affiliations

Biomedical Science Program, Department of Microbiology Graduate Program, Interdisciplinary Graduate Program in Translational Biomedicine, Medical Scientist Training Program

Center, Program and Institute Affiliations

George M. O’Brien Kidney Research Center, Institute for Clinical and Translational Science

Research Summary

In both intestinal and extraintestinal infections due to enterobacteria, adhesion of the bacteria to the host tissue is a necessary prerequisite to colonization and infection. Current research projects involve:

1) analysis of the genetic control of fimbrial expression in enteric bacteria,

2) the construction of recombinant plasmids encoding functional fimbriae (pili) and examination of gene products,

3) cloning, by recombinant DNA technology, fimbrial genes from members of the enterobacteriaceae, and

4) examination of recombinant strains for the acquisition of enhanced virulence properties.

Using the techniques of molecular biology the genes coding for fimbrial expression are being studied. Comparison of the structural and regulatory components of this genetic system in different genera of enterobacteria possessing diverse fimbrial antigens is being performed. Many genera of the enteric group of bacteria possess fimbriae which are antigenically distinct, and are expressed only under defined conditions in vitro. A comparison of the genetic elements encoding different types of fimbriae is being performed using gene cloning, DNA sequencing and hybridization, recombinant plasmids and DNA fusion molecules.

Publications

Clegg, S., Murphy, C. N. (2017). Epidemiology and virulence of Klebsiella pneumoniae. In M. A. Mulvey , D. J. Klumpp , A. E. Stapleton (Eds.) Urinary Tract Infections: Molecular Pathogenesis and Clinical Management. (2nd edition) ASM Press. DOI: 10.1128/microbiolspec.UTI-0005-2012.

Clegg, S., Murphy, C. N. (2016). Epidemiology and Virulence of Klebsiella pneumoniae. Microbiol Spectr, 4(1). PMID: 26999397.

Murphy, C. N., Mortensen, M. S., Krogfelt, K. A. & Clegg, S. (2013). Role of Klebsiella pneumoniae type 1 and type 3 fimbriae in colonizing silicone tubes implanted into the bladder of mice as a model of catheter-associated urinary tract infections. Infect Immun, 81(8), 3009-17. PMID: 23753626.

Zeiner, S. A., Dwyer, B. E. & Clegg, S. (2013). FimY does not interfere with FimZ-FimW interaction during type 1 fimbria production by Salmonella enterica serovar Typhimurium. Infect Immun, 81(12), 4453-60. PMID: 24042120.

Stahlhut, S. G., Chattopadhyay, S., Kisiela, D. I., Hvidtfeldt, K., Clegg, S., Struve, C., Sokurenko, E. V. & Krogfelt, K. A. (2013). Structural and population characterization of MrkD, the adhesive subunit of type 3 fimbriae. J Bacteriol, 195(24), 5602-13. PMID: 24123820.

Kisiela, D. I., Chattopadhyay, S., Tchesnokova, V., Paul, S., Weissman, S. J., Medenica, I., Clegg, S. & Sokurenko, E. V. (2013). Evolutionary Analysis Points to Divergent Physiological Roles of Type 1 Fimbriae in Salmonella and Escherichia coli. MBio, 4(2), e00625-12. PMID: 23462115.

Childers, B. M., Van Laar, T. A., You, T., Clegg, S. & Leung, K. P. (2013). MrkD1P from Klebsiella pneumoniae strain IA565 allows for coexistence with Pseudomonas aeruginosa and protection from protease-mediated biofilm detachment. Infect Immun, 81(11), 4112-20. PMID: 23980108.

Sterzenbach, T., Nguyen, K. T., Nuccio, S. P., Winter, M. G., Vakulskas, C. A., Clegg, S., Romeo, T. & Bäumler, A. J. (2013). A novel CsrA titration mechanism regulates fimbrial gene expression in Salmonella typhimurium. EMBO J, 32(21), 2872-83. PMID: 24056837.

Rêgo, A. T., Johnson, J. G., Geibel, S., Enguita, F. J., Clegg, S. & Waksman, G. (2012). Crystal structure of the MrkD(1P) receptor binding domain of Klebsiella pneumoniae and identification of the human collagen V binding interface. Mol Microbiol, 86(4), 882-93. PMID: 22988966.

Zeiner, S. A., Dwyer, B. E. & Clegg, S. (2012). FimA, FimF, and FimH are necessary for assembly of type 1 fimbriae on Salmonella enterica serovar Typhimurium. Infect Immun, 80(9), 3289-96. PMID: 22778099.

Kisiela, D. I., Chattopadhyay, S., Libby, S. J., Karlinsey, J. E., Fang, F. C., Tchesnokova, V., Kramer, J. J., Beskhlebnaya, V., Samadpour, M., Grzymajlo, K., Ugorski, M., Lankau, E. W., Mackie, R. I., Clegg, S. & Sokurenko, E. V. (2012). Evolution of Salmonella enterica virulence via point mutations in the fimbrial adhesin. PLoS Pathog, 8(6), e1002733. PMID: 22685400.

Murphy, C. N., Clegg, S. (2012). Klebsiella pneumoniae and type 3 fimbriae: nosocomial infection, regulation and biofilm formation. Future Microbiol, 7(8), 991-1002. PMID: 22913357.

Dwyer, B. E., Newton, K. L., Kisiela, D., Sokurenko, E. V. & Clegg, S. (2011). Single nucleotide polypmorphisms of fimH associated with adherence and biofilm formation by serovars of Salmonella enterica. Microbiology, 157(Pt 11), 3162-71. PMID: 21852351.

Johnson, J., Murphy, C., Sippy, J. & Clegg, S. (2011). Regulation of Klebsiella pneumoniae capsular expression and serum sensitivity by the Cyclic-di-GMP binding protein MrkH. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S., Wilson, J. & Johnson, J. (2011). More than one way to control hair growth: regulatory mechanisms in enterobacteria that affect fimbriae assembled by the chaperone/usher pathway. J Bacteriol, 193(9), 2081-8. PMID: 21398554.

Kisiela, D. I., Kramer, J. J., Tchesnokova, V., Aprikian, P., Yarov-Yarovoy, V., Clegg, S. & Sokurenko, E. V. (2011). Allosteric catch bond properties of the FimH adhesin from Salmonella enterica serovar Typhimurium. J Biol Chem, 286(44), 38136-47. PMID: 21795699.

Johnson, J. G., Murphy, C. N., Sippy, J., Johnson, T. J. & Clegg, S. (2011). Type 3 fimbriae and biofilm formation are regulated by the transcriptional regulators MrkHI in Klebsiella pneumoniae. J Bacteriol, 193(14), 3453-60. PMID: 21571997.

Clegg, S., Dwyer, B., Ynofski, A., Kisiela, D. & Sokurenko, E. (2010). Adherence and biofilm formation by Salmonella serovars are invluenced by fimH allelic variation of the fim gene cluster. Abstr Ann Meet Amer Soc Microbiol.

Johnson, J. G., Clegg, S. (2010). Role of MrkJ, a phosphodiesterase, in type 3 fimbrial expression and biofilm formation in Klebsiella pneumoniae. J Bacteriol, 192(15), 3944-50. PMID: 20511505.

Johnson, J., Clegg, S. (2009). The effect of a LuxR-like protein and an EAL domain protein on type 3 fimbrial production in Klebsiella pneumoniae. Abstr ASM Meeting on Biofilms, Cancun, Mexico.

Rosen, D. A., Pinkner, J. S., Jones, J. M., Walker, J. N., Clegg, S. & Hultgren, S. J. (2008). Utilization of an intracellular bacterial community pathway in Klebsiella pneumoniae urinary tract infection and the effects of FimK on type 1 pilus expression. Infect Immun, 76(7), 3337-45. PMID: 18411285.

Lau, H. Y., Clegg, S. & Moore, T. A. (2007). Identification of Klebsiella pneumoniae genes uniquely expressed in a strain virulent using a murine model of bacterial pneumonia. Microb Pathog, 42(4), 148-55. PMID: 17369011.

Mehling, J. S., Lavender, H. & Clegg, S. (2007). A Dam methylation mutant of Klebsiella pneumoniae is partially attenuated. FEMS Microbiol Lett, 268(2), 187-93. PMID: 17328745.

Nuccio, S. P., Chessa, D., Weening, E. H., Raffatellu, M., Clegg, S. & Bäumler, A. J. (2007). SIMPLE approach for isolating mutants expressing fimbriae. Appl Environ Microbiol, 73(14), 4455-62. PMID: 17526787.

Mehling, J., Hauer, S. & Clegg, S. (2007). The MrkGE two-component sensory system influences type 3 fimbrial expression under aerobic conditions and affects biofilm formation on human-derived extracellular matrices. Abstr Ann Meet Amer Soc Microbiol.

Boddicker, J. D., Anderson, R. A., Jagnow, J. & Clegg, S. (2006). Signature-tagged mutagenesis of Klebsiella pneumoniae to identify genes that influence biofilm formation on extracellular matrix material. Infect Immun, 74(8), 4590-7. PMID: 16861646.

McCarthy, T. R., Torrelles, J. B., MacFarlane, A. S., Katawczik, M., Kutzbach, B., Desjardin, L. E., Clegg, S., Goldberg, J. B. & Schlesinger, L. S. (2005). Overexpression of Mycobacterium tuberculosis manB, a phosphomannomutase that increases phosphatidylinositol mannoside biosynthesis in Mycobacterium smegmatis and mycobacterial association with human macrophages. Mol Microbiol, 58(3), 774-90. PMID: 16238626.

Boddicker, J., Anderson, R. & Clegg, S. (2005). Signature-tagged mutagenesis of Klebsiella pneumoniae to identify genes required for biofilm formation. Abstr Ann Meet Amer Soc Microbiol.

Lavender, H., Jagnow, J. J. & Clegg, S. (2005). Klebsiella pneumoniae type 3 fimbria-mediated immunity to infection in the murine model of respiratory disease. Int J Med Microbiol, 295(3), 153-9. PMID: 16047414.

Esteves, C. L., Jones, B. D. & Clegg, S. (2005). Biofilm formation by Salmonella enterica serovar Typhimurium and Escherichia coli on epithelial cells following mixed inoculations. Infect Immun, 73(8), 5198-203. PMID: 16041039.

Lavender, H. F., Jagnow, J. R. & Clegg, S. (2004). Biofilm formation in vitro and virulence in vivo of mutants of Klebsiella pneumoniae. Infect Immun, 72(8), 4888-90. PMID: 15271955.

Jagnow, J., Clegg, S. (2003). Klebsiella pneumoniae MrkD-mediated biofilm formation on extracellular matrix- and collagen-coated surfaces. Microbiology, 149(Pt 9), 2397-405. PMID: 12949165.

Boddicker, J. D., Ledeboer, N. A., Jagnow, J., Jones, B. D. & Clegg, S. (2002). Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster. Mol Microbiol, 45(5), 1255-65. PMID: 12207694.

Jagnow, J., Clegg, S. (2002). Klebsiella pneumoniae fimbria-mediated biofilm development on collagen coated and non-collagen coated surfaces. Abstr Ann Meet Amer Soc Microbiol.

Yeh, K. S., Tinker, J. K. & Clegg, S. (2002). FimZ binds the Salmonella typhimurium fimA promoter region and may regulate its own expression with FimY. Microbiol Immunol, 46(1), 1-10. PMID: 11911183.

Clegg, S., Hughes, K. T. (2002). FimZ is a molecular link between sticking and swimming in Salmonella enterica serovar Typhimurium. J Bacteriol, 184(4), 1209-13. PMID: 11807085.

Jagnow, J., Clegg, S. (2001). Klebsiella pneumoniae fimbria-mediated biofilm development on collagen coated and non-collagen coated surfaces. Eighth Ann Midwest Path Meet, University of Illinois.

Clegg, S., Sebghati, T. A. (2001). Klebsiella pneumoniae. In M. Sussman (Eds.) Molecular Medical Microbiology. Academic Press.

Tinker, J. K., Clegg, S. (2001). Control of FimY translation and type 1 fimbrial production by the arginine tRNA encoded by fimU in Salmonella enterica serovar Typhimurium. Mol Microbiol, 40(3), 757-68. PMID: 11359580.

Ledeboer, N. A., Boddicker, J. D., Clegg, S. & Jones, B. D. (2001). Biofilm formation by Salmonella enterica Serovar Typhimurium on eucaryotic cells is dependent on allelic variation of the Salmonella fimH gene. Eighth Ann Midwest Path Meet, University of Illinois.

Langstraat, J., Bohse, M. & Clegg, S. (2001). Type 3 fimbrial shaft (MrkA) of Klebsiella pneumoniae, but not the fimbrial adhesin (MrkD), facilitates biofilm formation. Infect Immun, 69(9), 5805-12. PMID: 11500458.

Tinker, J. K., Hancox, L. S. & Clegg, S. (2001). FimW is a negative regulator affecting type 1 fimbrial expression in Salmonella enterica serovar typhimurium. J Bacteriol, 183(2), 435-42. PMID: 11133935.

Wilson, R. L., Elthon, J., Clegg, S. & Jones, B. D. (2000). Salmonella enterica serovars gallinarum and pullorum expressing Salmonella enterica serovar typhimurium type 1 fimbriae exhibit increased invasiveness for mammalian cells. Infect Immun, 68(8), 4782-5. PMID: 10899888.

Tinker, J. K., Clegg, S. (2000). Characterization of FimY as a coactivator of type 1 fimbrial expression in Salmonella enterica serovar Typhimurium. Infect Immun, 68(6), 3305-13. PMID: 10816478.

Tinker, J., Clegg, S. (1999). Construction and characterization of fimY and fimW mutants affecting type 1 fimbrial expression in Salmonella typhimurium. Gordon Research Conference on Microbial Adherence and Signalling. Newport, RI.

Sebghati, T. A., Clegg, S. (1999). Construction and characterization of mutations within the Klebsiella mrkD1P gene that affect binding to collagen type V. Infect Immun, 67(4), 1672-6. PMID: 10085002.

Tinker, J., Hancox, L. & Clegg, S. (1998). The role of fimY in the regulation of Salmonella typhimurium FimA expression. pp. B94. Abstr Ann Meet Amer Soc Microbiol.

Sebghati, T. A., Korhonen, T. K., Hornick, D. B. & Clegg, S. (1998). Characterization of the type 3 fimbrial adhesins of Klebsiella strains. Infect Immun, 66(6), 2887-94. PMID: 9596764.

Tarkkanen, A. M., Virkola, R., Clegg, S. & Korhonen, T. K. (1997). Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells. Infect Immun, 65(4), 1546-9. PMID: 9119502.

Hancox, L. S., Yeh, K. S. & Clegg, S. (1997). Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium. FEMS Immunol Med Microbiol, 19(4), 289-96. PMID: 9537754.

Clegg, S., Hancox, L. S. & Yeh, K. S. (1996). Salmonella typhimurium fimbrial phase variation and FimA expression. J Bacteriol, 178(2), 542-5. PMID: 8550478.

Schurtz, T. A., Hornick, D. B. & Clegg, S. (1995). Conservation of the mrkD1c adhesin gene of Klebsiellea pnemophila IA565 among several type 3 fimbriate strains. Gordon Research Conference on Microbial Adherence and Signalling. Newport, RI.

Hornick, D. B., Thommandru, J., Smits, W. & Clegg, S. (1995). Adherence properties of an mrkD-negative mutant of Klebsiella pneumoniae. Infect Immun, 63(5), 2026-32. PMID: 7729917.

Yeh, K. S., Hancox, L. S. & Clegg, S. (1995). Construction and characterization of a fimZ mutant of Salmonella typhimurium. J Bacteriol, 177(23), 6861-5. PMID: 7592479.

Clegg, S., Swenson, D. L. (1994). Salmonella fimbriae. In P. Klemm (Eds.) Fimbriae: Adhesion, Biogenesis, Genetics, and Vaccines. pp. 105-14. CRC Press, Inc..

Schurtz, T. A., Clegg, S. (1994). The type 3 fimbrial adhesin gene, mrkD, is not conserved within enterobacteria. pp. D131. Abstr Ann Meet Amer Soc Microbiol.

Madison, B., Ofek, I., Clegg, S. & Abraham, S. N. (1994). Type 1 fimbrial shafts of Escherichia coli and Klebsiella pneumoniae influence sugar-binding specificities of their FimH adhesins. Infect Immun, 62(3), 843-8. PMID: 7906676.

Swenson, D. L., Kim, K. J., Six, E. W. & Clegg, S. (1994). The gene fimU affects expression of Salmonella typhimurium type 1 fimbriae and is related to the Escherichia coli tRNA gene argU. Mol Gen Genet, 244(2), 216-8. PMID: 7914347.

Schurtz, T. A., Hornick, D. B., Korhonen, T. K. & Clegg, S. (1994). The type 3 fimbrial adhesin gene (mrkD) of Klebsiella species is not conserved among all fimbriate strains. Infect Immun, 62(10), 4186-91. PMID: 7927674.

Hornick, D., Thommandru, J., Smits, W. & Clegg, S. (1994). Adherence to human buccal cells by type 3 fimbriate Klebsiella pneumoniae. pp. B338. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S., Korhonen, T. K., Hornick, D. B. & Tarkkanen, A. M. (1994). Type 3 fimbriae of the Enterobacteriaceae. In P. Klemm (Eds.) Fimbriae: Adhesion, Biogenesis, Genetics, and Vaccines. pp. 97-104. CRC Press, Inc..

Clegg, S., Swenson, D. L. & Convey, L. (1993). Characterization of the genes encoding type 1 fimbriae of Salmonella typhimurium. pp. D105. Abstr Ann Meet Amer Soc Microbiol.

Kukkonen, M., Raunio, T., Virkola, R., Lähteenmäki, K., Mäkelä, P. H., Klemm, P., Clegg, S. & Korhonen, T. K. (1993). Basement membrane carbohydrate as a target for bacterial adhesion: binding of type I fimbriae of Salmonella enterica and Escherichia coli to laminin. Mol Microbiol, 7(2), 229-37. PMID: 8095317.

Nichols, N., Clegg, S. (1993). Control of fimA expression in Klebsiella pneumoniae and comparison to the Escherichia coli system. pp. D107. Abstr Ann Meet Amer Soc Microbiol.

Swenson, D. L., Clegg, S. (1992). Expression of the Salmonella typhimurium fimA gene is affected by two ancillary fim determinants located downstream of fimA. pp. D284. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S. (1992). Microbial virulences as strategies to develop vaccines. In R. Isaacson (Eds.) Recombinant DNA vaccines: Rationale and strategies. Marcel Dekker Inc..

Tarkkanen, A. M., Allen, B. L., Williams, P. H., Kauppi, M., Haahtela, K., Siitonen, A., Orskov, I., Orskov, F., Clegg, S. & Korhonen, T. K. (1992). Fimbriation, capsulation, and iron-scavenging systems of Klebsiella strains associated with human urinary tract infection. Infect Immun, 60(3), 1187-92. PMID: 1347287.

Swenson, D. L., Clegg, S. (1992). Identification of ancillary fim genes affecting fimA expression in Salmonella typhimurium. J Bacteriol, 174(23), 7697-704. PMID: 1360005.

Hornick, D. B., Allen, B. L., Horn, M. A. & Clegg, S. (1992). Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products. Infect Immun, 60(4), 1577-88. PMID: 1312518.

Nichols, N. N., Clegg, S. (1991). Deletion of the fimI gene reduces phenotypic expression of Klebsiella pneumoniae type 1 fimbriae. pp. D195. Abstr Ann Meet Amer Soc Microbiol.

Hornick, D. B., Allen, B. L., Horn, M. A. & Clegg, S. (1991). Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae. J Clin Microbiol, 29(9), 1795-800. PMID: 1685495.

Allen, B. L., Gerlach, G. F. & Clegg, S. (1991). Nucleotide sequence and functions of mrk determinants necessary for expression of type 3 fimbriae in Klebsiella pneumoniae. J Bacteriol, 173(2), 916-20. PMID: 1670938.

Swenson, D. L., Clegg, S. & Old, D. C. (1991). The frequency of fim genes among Salmonella serovars. Microb Pathog, 10(6), 487-92. PMID: 1686630.

Nichols, N. N., Bohan, T. & Clegg, S. (1990). Transcription of fim genes in Klebsiella pneumoniae. pp. 38. Abstr Microbial Adherence and Invasion. Panama City, FL.

Tarrkanen, A. M., Allen, B. L., Westerlund, B., Holthofer, H., Kuusela, P., Risteli, L., Clegg, S. & Korhnomen, T. K. (1990). Type V collagen as tissue target for type 3 fimbriae of enteric bacteria. pp. 37. Abstr Microbial Adherence and Invasion. Panama City, FL.

Nichols, W. A., Clegg, S. & Brown, M. (1990). Molecular characterization of the fimA gene of Serratia marcescens. pp. 39. Abstr Microbial Adherence and Invasion. Panama City, FL.

Hornick, D. L., Allen, B. L., Koerner, T. A. & Clegg, S. (1990). Adherence of K. pneumoniae to human trachael epithelial cells. pp. D186. Abstr Ann Meet Amer Soc Microbiol.

Allen, B. L., Hornick, D., Ginkel, D. & Clegg, S. (1990). Characterization of localized bacterial adherence to human lung mediated by various fimbrial types of the Enterobacteriaceae. pp. 8. Abstr Microbial Adherence and Invasion. Panama City, FL.

Tarkkanen, A. M., Allen, B. L., Westerlund, B., Holthöfer, H., Kuusela, P., Risteli, L., Clegg, S. & Korhonen, T. K. (1990). Type V collagen as the target for type-3 fimbriae, enterobacterial adherence organelles. Mol Microbiol, 4(8), 1353-61. PMID: 1980713.

Nichols, W. A., Clegg, S. & Brown, M. R. (1990). Characterization of the type 1 fimbrial subunit gene (fimA) of Serratia marcescens. Mol Microbiol, 4(12), 2119-26. PMID: 1982455.

Korhonen, T. K., Westerlund, B., Tarkkanen, A. M., Sareneva, T., Virkola, R., Kuuscla, R., Holthofer, H., van Die, I., Hoekstra, W., Allen, B. L. & Clegg, S. (1989). Binding of enterobacterial fimbriae to proteins of basement membranes and connective tissue - a novel function for fimbriae. In FEMS Workshop on Microbial Surface Antigens and Toxins.

Allen, B. L., Gerlach, G. F. & Clegg, S. (1989). Characterization of the type 3 (MR/K) fimbrial gene cluster of K. pneumoniae. pp. D220. Abstr Ann Meet Amer Soc Microbiol.

Swenson, D. L., Ness, N. J. & Clegg, S. (1989). Construction and expression of fimA-lacZ fusions of K. pneumoniae and S. typhimurium. pp. D45. Abstr Ann Meet Amer Soc Microbiol.

Gerlach, G. F., Allen, B. L. & Clegg, S. (1989). Type 3 fimbriae among enterobacteria and the ability of spermidine to inhibit MR/K hemagglutination. Infect Immun, 57(1), 219-24. PMID: 2562835.

Gerlach, G. F., Clegg, S. & Allen, B. L. (1989). Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae. J Bacteriol, 171(3), 1262-70. PMID: 2563996.

Crichton, P. B., Yakubu, D. E., Old, D. C. & Clegg, S. (1989). Immunological and genetical relatedness of type-1 and type-2 fimbriae in salmonellas of serotypes Gallinarum, Pullorum and Typhimurium. J Appl Bacteriol, 67(3), 283-91. PMID: 2575611.

Gerlach, G. F., Clegg, S., Ness, N. J., Swenson, D. L., Allen, B. L. & Nichols, W. A. (1989). Expression of type 1 fimbriae and mannose-sensitive hemagglutinin by recombinant plasmids. Infect Immun, 57(3), 764-70. PMID: 2563717.

Gerlach, G. F., Clegg, S. (1988). Cloning and characterization of the gene cluster encoding type 3 (MR/K) fimbriae of Klebsiella pneumoniae. FEMS Microbiol Lett, 49(3), 377-83.

Gerlach, G. F., Swenson, D. L. & Clegg, S. (1988). Expression of serologically distinct fimbrial subunits by bacteria possessing recombinant plasmids carrying the genes for Klebsiella type 1 and type 3 fimbriae. pp. D198. Abstr Ann Meet Amer Soc Microbiol.

Gerlach, G. F., Clegg, S. (1988). Characterization of two genes encoding antigenically distinct type-1 fimbriae of Klebsiella pneumoniae. Gene, 64(2), 231-40. PMID: 2456966.

Gerlach, G. F., Allen, B. L. & Clegg, S. (1988). Molecular characterization of the type 3 (MR/K) fimbriae of Klebsiella pneumoniae. J Bacteriol, 170(8), 3547-53. PMID: 2900237.

Gerlach, G. F., Clegg, S. & Nichols, W. A. (1988). Characterization of the genes encoding urease activity of Klebsiella pneumoniae. FEMS Microbiol Lett, 50(2-3), 131-5.

Purcell, B. K., Gerlach, G. F. & Clegg, S. (1987). Comparison of type 1 fimbrial gene clusters from clinical isolates of Klebsiella pneumoniae. pp. D57. Abstr Ann Meet Amer Soc Microbiol.

Nichols, W., Gerlach, G. F. & Clegg, S. (1987). Cloning of the genes encoding urease production of a uropathogenic Klebsiella pneumoniae strain. pp. D115. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S., Purcell, B. K. & Pruckler, J. (1987). Characterization of genes encoding type 1 fimbriae of Klebsiella pneumoniae, Salmonella typhimurium, and Serratia marcescens. Infect Immun, 55(2), 281-7. PMID: 2879791.

Clegg, S., Gerlach, G. F. (1987). Enterobacterial fimbriae. J Bacteriol, 169(3), 934-8. PMID: 2880835.

Purcell, B. K., Pruckler, J. & Clegg, S. (1987). Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium. J Bacteriol, 169(12), 5831-4. PMID: 2890624.

Gerlach, G., Clegg, S. (1986). Hemagglutinating and adherence characteristics of surface antigens isolated from Klebsiella pneumoniae. 26th ACAAC Meeting, New Orleans, Louisiana.

Pierce, J. K., Clegg, S. (1986). Fimbrial expression and mannose-resistant hemagglutinating properties of Escherichia coli strains possessing recombinant plasmids. pp. D114. Abstr Ann Meet Amer Soc Microbiol.

Purcell, B. K., Clegg, S. & Pruckler, J. (1985). Complementation by type 1 fimbrial genes of the Enterobacteriaceae. pp. D97. Abstr Ann Meet Amer Soc Microbiol.

Hull, S., Clegg, S., Sanborg Eden, C. & Hull, R. (1985). Multiple forms of genes in pyelonephritogenic Escherichia coli encoding adhesins binding globoseries glycolipid receptors. Infect Immun, 47(1), 80-3. PMID: 2856916.

Clegg, S., Hull, S., Hull, R. & Pruckler, J. (1985). Construction and comparison of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae. Infect Immun, 48(2), 275-9. PMID: 2580786.

Clegg, S., Pruckler, J. & Purcell, B. K. (1985). Complementation analyses of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae. Infect Immun, 50(1), 338-40. PMID: 2864314.

Evans, D. G., Evans, D. J., Sack, D. A. & Clegg, S. (1984). Enterotoxigenic Escherichia coli pathogenic for man: biological and immunological aspects of fimbrial colonization factor antigens. In E. Boedeker (Eds.) Attachment of Organisms to the Gut Mucosa. pp. 63-78. CRC Press, Inc..

Pierce, J., Clegg, S. (1984). Nucleotide sequence studies of a plasmid encoding mannose-resistant fimbriae. pp. B56. Abstr Ann Meet Amer Soc Microbiol.

Pierce, J., Clegg, S. (1983). Identification of the genetic determinants coding for expression of mannose-resistant fimbriae in Escherichia coli. pp. B46. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S., Pierce, J. K. (1983). Organization of genes responsible for the production of mannose-resistant fimbriae of a uropathogenic Escherichia coli isolate. Infect Immun, 42(3), 900-6. PMID: 6139340.

Purcell, B. K., Clegg, S. (1983). Construction and expression of a recombinant plasmid encoding type 1 fimbriae of Klebsiella pneumoniae. pp. B77. Abstr Ann Meet Amer Soc Microbiol.

Purcell, B. K., Clegg, S. (1983). Construction and expression of recombinant plasmids encoding type 1 fimbriae of a urinary Klebsiella pneumoniae isolate. Infect Immun, 39(3), 1122-7. PMID: 6132874.

Clegg, S. (1982). Cloning of the genes determining the production of mannose-resistant fimbriae (pili) in Escherichia coli. pp. B110. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S. (1982). Serological heterogeneity among fimbrial antigens causing mannose-resistant hemagglutination by uropathogenic Escherichia coli. Infect Immun, 35(2), 745-8. PMID: 6173329.

Clegg, S., Evans Jr, D. J. & Evans, D. G. (1982). Antigenic heterogeneity of haemagglutination type VI fimbriae produced by Escherichia coli isolated from patients with bacteremia. J Clin Microbiol, 16(1), 174-80. PMID: 6179963.

Clegg, S. (1982). Cloning of genes determining the production of mannose-resistant fimbriae in a uropathogenic strain of Escherichia coli belonging to serogroup O6. Infect Immun, 38(2), 739-44. PMID: 6128310.

Clegg, S., Evans, D. J. & Evans, D. G. (1980). Antigenic analysis of fimbriae produced by bacteremia-associated Escherichia coli. pp. B44. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S., Evans, D. G. & Evans, D. J. (1980). Enzyme-linked immunosorbent assay for quantitating the humoral immune response to the colonization factor antigen of enterotoxigenic Escherichia coli. Infect Immun, 27(2), 525-31. PMID: 6103870.

Evans, D. G., Evans Jr, D. J. & Clegg, S. (1980). Detection of enterotoxigenic Escherichia coli colonization factor antigen I in stool specimens by an enzyme-linked immunosorbent assay. J Clin Microbiol, 12(6), 738-43. PMID: 7031075.

Evans, D. J., Evans, D. G. & Clegg, S. (1979). Antigenic heterogeneity of mannose-sensitive hemagglutinins (MSHAs, fimbriae, or pili) of enteropathogenic Escherichia coli (EPEC). pp. B17. Abstr Ann Meet Amer Soc Microbiol.

Clegg, S., Old, D. C. (1979). Fimbriae of Escherichia coli K-12 strain AW405 and related bacteria. J Bacteriol, 137(2), 1008-12. PMID: 370087.

Duguid, J. P., Clegg, S. & Wilson, M. I. (1979). The fimbrial and non-fimbrial haemagglutinins of Escherichia coli. J Med Microbiol, 12(2), 213-27. PMID: 379341.

Evans, D. G., Evans Jr, D. J., Clegg, S. & Pauley, J. A. (1979). Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun, 25(2), 738-48. PMID: 39896.