Wendy J. Maury, PhD

Portrait
Professor of Microbiology and Immunology

Contact Information

Office: 3-750 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
319-335-8021

Lab: 3-701E Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
319-335-7613

Education

BA, Botany, Duke University
MS, Botany, North Carolina State University
PhD, Biology, University of Virginia

Post Doctoral Fellow, Molecular Microbiology, National Institutes of Health-NIAID
Post Doctoral Fellow, Virology, National Institutes of Health-NIAID, Rocky Mountain Laboratories

Education/Training Program Affiliations

Biomedical Science Program, Department of Microbiology Graduate Program, Interdisciplinary Graduate Program in Molecular and Cellular Biology, Interdisciplinary Graduate Program in Translational Biomedicine, Medical Scientist Training Program

Center, Program and Institute Affiliations

Center for Gene Therapy of Cystic Fibrosis and other Genetic Diseases, Center for Immunology and Immune-based Diseases, Helen C. Levitt Center for Viral Pathogenesis, Holden Comprehensive Cancer Center

Research Summary

A goal of our research is to understand how enveloped viral glycoproteins bind to and mediate entry into permissive cells. An appreciation of the cellular attachment factors, receptors, internalization pathways and fusion events used by a virus to enter cells provides an avenue for the development of antiviral therapies. To this end, my laboratory primarily focuses on the filoviruses, Ebolavirus and Marburgvirus, but also investigates entry mechanisms of human immunodeficiency virus-1 (HIV-1). We have recently identified a novel cell surface receptor for filoviruses and are currently exploring the role of this family of cellular receptors in filovirus pathogenesis. In addition, studies in the lab have helped to elucidate the endosomal pathways used by filoviruses for entry and identified Ebola virus glycoprotein amino acids that are critical for productive Ebola virus infection. Stemming from this work are investigations into novel vaccine and antiviral therapeutic approaches designed to block filovirus infection.

Publications

Das, A., Hirai-Yuki, A., González-López, O., Rhein, B., Moller-Tank, S., Brouillette, R., Hensley, L., Misumi, I., Lovell, W., Cullen, J. M., Whitmire, J. K., Maury, W. J. & Lemon, S. M. (2017). TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions. MBio, 8(5), e00969-17. PMID: 28874468.

Davey, R. A., Shtanko, O., Anantpadma, M., Sakurai, Y., Chandran, K. & Maury, W. J. (2017). Mechanisms of Filovirus Entry. Curr Top Microbiol Immunol, Epub ahead of print. PMID: 28601947.

Lennemann, N. J., Herbert, A. S., Brouillette, R., Rhein, B., Bakken, R. A., Perschbacher, K. J., Cooney, A. L., Miller-Hunt, C. L., Ten Eyck, P., Biggins, J., Olinger, G., Dye, J. M. & Maury, W. J. (2017). Vesicular stomatitis virus pseudotyped with Ebola virus glycoprotein serves as a protective, non-infectious vaccine against Ebola virus challenge in mice. J Virol, 91(17), e00479-17. PMID: 28615211.

Brouillette, R. B., Phillips, E. K., Ayithan, N. & Maury, W. J. (2017). Differences in Glycoprotein Complex Receptor Binding Site Accessibility Prompt Poor Cross-Reactivity of Neutralizing Antibodies between Closely Related Arenaviruses. J Virol, 91(7), e01454-16. PMID: 28100617.

Brouillette, R. B., Maury, W. J. (2017). Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms. Methods Mol Biol, 1628, 53-63. PMID: 28573610.

Sinn, P. L., Coffin, J. E., Ayithan, N., Holt, K. H. & Maury, W. J. (2017). Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins. In Methods Mol Biol. pp. 65-78. PMID: 28573611.

Anantpadma, M., Kouznetsova, J., Wang, H., Huang, R., Kolokoltsov, A., Guha, R., Lindstrom, A. R., Shtanko, O., Simeonov, A., Maloney, D. J., Maury, W., LaCount, D. J., Jadhav, A. & Davey, R. A. (2016). Large-Scale Screening and Identification of Novel Ebola Virus and Marburg Virus Entry Inhibitors. Antimicrob Agents Chemother, 60(8), 4471-81. PMID: 27161622.

Rhein, B. A., Brouillette, R. B., Schaack, G. A., Chiorini, J. A. & Maury, W. (2016). Characterization of Human and Murine T-Cell Immunoglobulin Mucin Domain 4 (TIM-4) IgV Domain Residues Critical for Ebola Virus Entry. J Virol, 90(13), 6097-111. PMID: 27122575.

Rhein, B. A., Powers, L. S., Rogers, K., Anantpadma, M., Singh, B. K., Sakurai, Y., Bair, T., Miller-Hunt, C., Sinn, P., Davey, R. A., Monick, M. M. & Maury, W. (2015). Interferon-? Inhibits Ebola Virus Infection. PLoS Pathog, 11(11), e1005263. PMID: 26562011.

Lennemann, N. J., Walkner, M., Berkebile, A. R., Patel, N. & Maury, W. (2015). The Role of Conserved N-Linked Glycans on Ebola Virus Glycoprotein 2. J Infect Dis, 212(Suppl 2), S204-9. PMID: 26038399.

Rhein, B. A., Maury, W. J. (2015). Ebola virus entry into host cells: identifying therapeutic strategies. Curr Clin Microbiol Rep, 2(3), 115-124. PMID: 26509109.

Moller-Tank, S., Maury, W. J. (2015). Ebola virus entry: a curious and complex series of events. PLoS Pathogens, 11(4), e1004731. PMID: 25928849.

Moller-Tank, S., Maury, W. J. (2014). Phosphatidylserine receptors: Enhancers of enveloped virus entry and infection. Virology, 468-470C, 565-80. PMID: 25277499.

Moller-Tank, S., Albritton, L. M., Rennert, P. D. & Maury, W. J. (2014). Characterizing functional domains for TIM-mediated enveloped virus entry. J Virol, 88(12), 6702-13. PMID: 24696470.

Lennemann, N. J., Rhein, B. A., Ngundo, E., Chandran, K., Liu, Q. & Maury, W. J. (2014). Comprehensive functional analysis of N-linked glycans on Ebola virus GP1. MBio, 5(1), e00862-13. PMID: 24473128.

Li, M., Ablan, S., Miao, C., Zheng, Y. M., Fuller, M. S., Rennert, P. D., Maury, W. J., Johnson, M. C., Freed, E. O. & Liu, S. L. (2014). TIM-family proteins inhibit HIV-1 release. Proc Natl Acad Sci U S A, 111(35), E3699-707. PMID: 25136083.

Oakland, M. F., Maury, W. J., McCray, Jr, P. B. & Sinn, P. L. (2013). Intrapulmonary versus nasal transduction of murine airways with GP64-pseudotyped viral vectors. Mol Ther Nucleic Acids, 2, e69. PMID: 23360952.

Jones, P. H., Maric, M., Madison, M. N., Maury, W. J., Roller, R. J. & Okeoma, C. M. (2013). BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1). Virology, 438, 37-49. PMID: 23411007.

Moller-Tank, S., Kondratowicz, A. S., Davey, R. A., Rennert, P. D. & Maury, W. J. (2013). Role of the phosphatidylserine receptor TIM-1 in enveloped virus entry. J Virol, 87(15), 8327-41. PMID: 23698310.

Kondratowicz, A. S., Hunt, C. L., Davey, R. A., Cherry, S. & Maury, W. J. (2013). AMP-Activated Protein Kinase Is Required for the Macropinocytic Internalization of Ebolavirus. J Virol, 87(2), 746-55. PMID: 23115293.

Hunt, C. L., Lennemann, N. J. & Maury, W. J. (2012). Filovirus entry: a novelty in the viral fusion world. Viruses, 4(2), 258-75. PMID: 22470835.

Kondratowicz, A. S., Maury, W. J. (2012). Ebolavirus: a brief review of novel therapeutic targets. Future Microbiol, 7(1), 1-4. PMID: 22191439.

Sloat, B. R., Kiguchi, K., Xiao, G., DeGiovanni, J., Maury, W. J. & Cui, Z. (2012). Transcutaneous DNA immunization following waxing-based hair depilation. J Control Release, 157(1), 94-102. PMID: 21907253.

Kraus, G. A., Mengwasser, J., Maury, W. J. & Oh, C. (2011). Synthesis of chroman aldehydes that inhibit HIV. Bioorg Med Chem Lett, 21(5), 1399-401. PMID: 21306897.

Brindley, M. A., Hunt, C. L., Kondratowicz, A. S., Bowman, J., Sinn, P. L., McCray, P. B., Quinn, K., Weller, M. L., Chiorini, J. A. & Maury, W. J. (2011). Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein. Virology, 415(2), 83-94. PMID: 21529875.

Oh, C., Price, J., Brindley, M. A., Widrlechner, M. P., Qu, L., McCoy, J. A., Murphy, P., Hauck, C. & Maury, W. J. (2011). Inhibition of HIV-1 infection by aqueous extracts of Prunella vulgaris L. Virol J, 8, 188. PMID: 21513560.

Kondratowicz, A. S., Lennemann, N. J., Sinn, P. L., Davey, R. A., Hunt, C. L., Moller-Tank, S., Meyerholz, D. K., Rennert, P., Mullins, R. F., Brindley, M., Sandersfeld, L. M., Quinn, K., Weller, M., McCray, P. B., Chiorini, J. & Maury, W. J. (2011). T-cell immunoglobulin and mucin domain 1 (TIM-1) is a receptor for Zaire Ebolavirus and Lake Victoria Marburgvirus. Proc Natl Acad Sci U S A, 108(20), 8426-31. PMID: 21536871.

Hunt, C. L., Kolokoltsov, A. A., Davey, R. A. & Maury, W. J. (2011). The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol, 85(1), 334-47. PMID: 21047970.

Birt, D. F., Widrlechner, M. P., Hammer, K. D., Hillwig, M. L., Wei, J., Kraus, G. A., Murphy, P. A., McCoy, J., Wurtele, E. S., Neighbors, J. D., Wiemer, D. F., Maury, W. J. & Price, J. P. (2009). Hypericum in infection: Identification of anti-viral and anti-inflammatory constituents. Pharm Biol, 47(8), 774-82. PMID: 19907671.

Maury, W. J., Oaks, J. L. (2009). Equine infectious anemia virus and macrophages. In M. Desport (Eds.) Lentiviruses and Macrophages. pp. Chapter 9. Horizon Press.

Maury, W. J., Price, J. P., Brindley, M. A., Oh, C., Neighbors, J. D., Wiemer, D. F., Wills, N., Carpenter, S., Hauck, C., Murphy, P., Widrlechner, M. P., Delate, K., Kumar, G., Kraus, G. A., Rizshsky, L. & Nikolau, B. (2009). Identification of light-independent inhibition of human immunodeficiency virus-1 infection through bioguided fractionation of Hypericum perforatum. Virol J, 6, 101. PMID: 19594941.

Brindley, M. A., Widrlechner, M. P., McCoy, J. A., Murphy, P., Hauck, C., Rizshsky, L., Nikolau, B. & Maury, W. J. (2009). Inhibition of lentivirus replication by aqueous extracts of Prunella vulgaris. Virol J, 6, 8. PMID: 19154592.

Quinn, K., Brindley, M. A., Weller, M. L., Kaludov, N., Kondratowicz, A., Hunt, C. L., Sinn, P. L., McCray, P. B., Stein, C. S., Davidson, B. L., Flick, R., Mandell, R., Staplin, W., Maury, W. J. & Chiorini, J. A. (2009). Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus pseudotyped vectors. J Virol, 83(19), 10176-86. PMID: 19625394.

Brindley, M. A., Zhang, B., Montelaro, R. C. & Maury, W. J. (2008). An equine infectious anemia virus variant superinfects cells through novel receptor interactions. J Virol, 82(19), 9425-32. PMID: 18667522.

Tallmadge, R. L., Brindley, M. A., Salmans, J., Mealey, R. H., Maury, W. J. & Carpenter, S. (2008). Development and characterization of an equine infectious anemia virus Env-pseudotyped reporter virus. Clin Vaccine Immunol, 15(7), 1138-40. PMID: 18448619.

Leontiev, V. V., Maury, W. J. & Hadany, L. (2008). Drug induced superinfection in HIV and the evolution of drug resistance. Infect Genet Evol, 8(1), 40-50. PMID: 18024234.

Brindley, M. A., Maury, W. J. (2008). Equine infectious anemia virus entry occurs through clathrin-mediated endocytosis. J Virol, 82(4), 1628-37. PMID: 18057237.

Birt, D. F., Widrlechner, M. P., Lalone, C. A., Wu, L., Bae, J., Solco, A. K., Kraus, G. A., Murphy, P. A., Wurtele, E. S., Leng, Q., Hebert, S. C., Maury, W. J. & Price, J. P. (2008). Echinacea in infection. Am J Clin Nutr, 87(2), 488S-92S. PMID: 18258644.

Sedore, S. C., Byers, S. A., Biglione, S., Price, J. P., Maury, W. J. & Price, D. H. (2007). Manipulation of P-TEFb control machinery by HIV: recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR. Nucleic Acids Res, 35(13), 4347-58. PMID: 17576689.

Biglione, S., Byers, S. A., Price, J. P., Nguyen, V. T., Bensaude, O., Price, D. H. & Maury, W. J. (2007). Inhibition of HIV-1 replication by P-TEFb inhibitors DRB, seliciclib and flavopiridol correlates with release of free P-TEFb from the large, inactive form of the complex. Retrovirology, 4, 47-56. PMID: 17625008.

Brindley, M. A., Hughes, L., Ruiz, A., McCray, P. B., Sanchez, A., Sanders, D. A. & Maury, W. J. (2007). Ebola virus glycoprotein 1: identification of residues important for binding and postbinding events. J Virol, 81(14), 7702-9. PMID: 17475648.

Sinn, P. L., Goreham-Voss, J. D., Arias, A. C., Hickey, M. A., Maury, W. J., Chikkanna-Gowda, C. P. & McCray, P. B. (2007). Enhanced gene expression conferred by stepwise modification of a nonprimate lentiviral vector. Hum Gene Ther, 18(12), 1244-52. PMID: 18052720.

Maury, W. J., Thompson, R. J., Jones, Q., Bradley, S., Denke, T., Baccam, P., Smazik, M. & Oaks, J. L. (2005). Evolution of the equine infectious anemia virus long terminal repeat during the alteration of cell tropism. J Virol, 79(9), 5653-64. PMID: 15827180.

Brindley, M. A., Maury, W. J. (2005). Endocytosis and a low-pH step are required for productive entry of equine infectious anemia virus. J Virol, 79(23), 14482-8. PMID: 16282447.

Hines, R., Sorensen, B. R., Shea, M. & Maury, W. J. (2004). PU.1 binding to ets motifs within the equine infectious anemia virus long terminal repeat (LTR) enhancer: regulation of LTR activity and virus replication in macrophages. J Virol, 78(7), 3407-18. PMID: 15016863.

Maury, W. J., Wright, P. J. & Bradley, S. (2003). Characterization of a cytolytic strain of equine infectious anemia virus. J Virol, 77(4), 2385-99. PMID: 12551976.

Shore, S. M., Byers, S. A., Maury, W. J. & Price, D. H. (2003). Identification of a novel isoform of Cdk9. Gene, 307, 175-82. PMID: 12706900.

Reed-Inderbitzin, E., Maury, W. J. (2003). Cellular specificity of HIV-1 replication can be controlled by LTR sequences. Virology, 314(2), 680-95. PMID: 14554095.

Hines, R., Maury, W. J. (2001). DH82 cells: a macrophage cell line for the replication and study of equine infectious anemia virus. J Virol Methods, 95(1-2), 47-56. PMID: 11377712.

Faga, B., Maury, W. J., Bruckner, D. A. & Grose, C. (2001). Identification and mapping of single nucleotide polymorphisms in the varicella-zoster virus genome. Virology, 280(1), 1-6. PMID: 11162813.

Maury, W. J., Bradley, S., Wright, B. & Hines, R. (2000). Cell specificity of the transcription-factor repertoire used by a lentivirus: motifs important for expression of equine infectious anemia virus in nonmonocytic cells. Virology, 267(2), 267-78. PMID: 10662622.

Maury, W. J. (1998). Regulation of equine infectious anemia virus expression. J Biomed Sci, 5(1), 11-23. PMID: 9570509.

Maury, W. J., Oaks, J. L. & Bradley, S. (1998). Equine endothelial cells support productive infection of equine infectious anemia virus. J Virol, 72(11), 9291-7. PMID: 9765477.

Maury, W. J., Perryman, S., Oaks, J. L., Seid, B. K., Crawford, T., McGuire, T. & Carpenter, S. (1997). Localized sequence heterogeneity in the long terminal repeats of in vivo isolates of equine infectious anemia virus. J Virol, 71(7), 4929-37. PMID: 9188555.

Maury, W. J., Carpenter, S., Graves, K. & Chesebro, B. (1994). Cellular and viral specificity of equine infectious anemia virus Tat transactivation. Virology, 200(2), 632-42. PMID: 8178449.

Maury, W. J. (1994). Monocyte maturation controls expression of equine infectious anemia virus. J Virol, 68(10), 6270-9. PMID: 8083967.

Biswas, P., Poli, G., Kinter, A. L., Justement, J. S., Stanley, S. K., Maury, W. J., Bressler, P., Orenstein, J. M. & Fauci, A. S. (1992). Interferon gamma induces the expression of human immunodeficiency virus in persistently infected promonocytic cells (U1) and redirects the production of virions to intracytoplasmic vacuoles in phorbol myristate acetate-differentiated U1 cells. J Exp Med, 176(3), 739-50. PMID: 1512539.

Potts, B. J., Maury, W. J. & Martin, M. A. (1990). Replication of HIV-1 in primary monocyte cultures. Virology, 175(2), 465-76. PMID: 2183468.

Chesebro, B., Wehrly, K. & Maury, W. J. (1990). Differential expression in human and mouse cells of human immunodeficiency virus pseudotyped by murine retroviruses. J Virol, 64(9), 4553-7. PMID: 2166831.

Kinter, A. L., Poli, G., Maury, W. J., Folks, T. M. & Fauci, A. S. (1990). Direct and cytokine-mediated activation of protein kinase C induces human immunodeficiency virus expression in chronically infected promonocytic cells. J Virol, 64(9), 4306-12. PMID: 2200885.

Maury, W. J., Potts, B. J. & Rabson, A. B. (1989). Infection of human placental tissue with HIV-1. In R. A. Lerner , H. Ginsberg & F. Brown (Eds.) Vaccines 89. Modern Approaches to New Vaccines Including Prevention of AIDS. pp. 133. Cold Spring Harbor Laboratory.

Duh, E. J., Maury, W. J., Folks, T. M., Fauci, A. S. & Rabson, A. B. (1989). Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat. Proc Natl Acad Sci U S A, 86(15), 5974-8. PMID: 2762307.

Koenig, S., Hirsch, V. M., Olmsted, R. A., Powell, D., Maury, W. J., Rabson, A., Fauci, A. S., Purcell, R. H. & Johnson, P. R. (1989). Selective infection of human CD4+ cells by simian immunodeficiency virus: productive infection associated with envelope glycoprotein-induced fusion. Proc Natl Acad Sci U S A, 86(7), 2443-7. PMID: 2784571.

Maury, W. J., Potts, B. J. & Rabson, A. B. (1989). HIV-1 infection of first-trimester and term human placental tissue: a possible mode of maternal-fetal transmission. J Infect Dis, 160(4), 583-8. PMID: 2477467.

Maury, W. J., Huber, S. C. & Moreland, D. E. (1981). Effects of Magnesium on Intact Chloroplasts : II. CATION SPECIFICITY AND INVOLVEMENT OF THE ENVELOPE ATPase IN (SODIUM) POTASSIUM/PROTON EXCHANGE ACROSS THE ENVELOPE. Plant Physiol, 68(6), 1257-63. PMID: 16662089.

Huber, S. C., Maury, W. J. (1980). Effects of Magnesium on Intact Chloroplasts: I. EVIDENCE FOR ACTIVATION OF (SODIUM) POTASSIUM/PROTON EXCHANGE ACROSS THE CHLOROPLAST ENVELOPE. Plant Physiol, 65(2), 350-4. PMID: 16661188.

Davis, J. N., Olender, E., Maury, W. J. & McDaniel, R. (1980). Alpha-adrenergic regulation of cholinergic responses in rat parotid acinar cells. Mol Pharmacol, 18(3), 356-61. PMID: 6258046.

Davis, J. N., Maury, W. J. (1978). Clonidine and related imidazolines are postsynaptic alpha adrenergic antagonists in dispersed rat parotid cells. J Pharmacol Exp Ther, 207(2), 425-30. PMID: 213554.