Vladimir Badovinac, PhD

Portrait
Associate Professor of Pathology
Associate Professor of Microbiology and Immunology

Contact Information

Primary Office: 3-550 Bowen Science Building
Iowa City, IA 52242
319-384-2930

Education

BS, Molecular Biology and Physiology, University of Belgrade, Yugoslavia
PhD, Immunology, Institute for Biological Research and School of Medicine, University of Belgrade, Yugoslavia

Postdoctoral Fellow, University of Iowa, Department of Microbiology

Education/Training Program Affiliations

Department of Pathology Graduate Program, Interdisciplinary Graduate Program in Immunology, Medical Scientist Training Program

Center, Program and Institute Affiliations

Center for Immunology and Immune-based Diseases, Holden Comprehensive Cancer Center

Research Summary

Regulation of antigen-specific CD8 T cell memory and homeostasis after infection or vaccination.

Publications

Van Braeckel-Budimir, N., Martin, M. D., Hartwig, S. M., Legge, K. L., Badovinac, V. P. & Harty, J. T. (2017). Antigen Exposure History Defines CD8 T Cell Dynamics and Protection during Localized Pulmonary Infections. Frontiers in immunology, 8, 40. PMID: 28191007.

Shan, Q., Zeng, Z., Xing, S., Li, F., Hartwig, S. M., Gullicksrud, J. A., Kurup, S. P., Van Braeckel-Budimir, N., Su, Y., Martin, M. D., Varga, S. M., Taniuchi, I., Harty, J. T., Peng, W., Badovinac, V. P. & Xue, H. H. (2017). The transcription factor Runx3 guards cytotoxic CD8<sup>+</sup> effector T cells against deviation towards follicular helper T cell lineage. Nature immunology. PMID: 28604718.

Martin, M. D., Shan, Q., Xue, H. H. & Badovinac, V. P. (2017). Time and Antigen-Stimulation History Influence Memory CD8 T Cell Bystander Responses. Frontiers in immunology, 8, 634. PMID: 28642758.

Gullicksrud, J. A., Li, F., Xing, S., Zeng, Z., Peng, W., Badovinac, V. P., Harty, J. T. & Xue, H. H. (2017). Differential Requirements for Tcf1 Long Isoforms in CD8<sup>+</sup> and CD4<sup>+</sup> T Cell Responses to Acute Viral Infection. Journal of immunology (Baltimore, Md. : 1950), 199(3), 911-919. PMID: 28652395.

Martin, M. D., Badovinac, V. P. (2016). Sifting through CD8<sup>+</sup> T Cell Memory. Immunity, 45(6), 1184-1186. PMID: 28002725.

Danahy, D. B., Strother, R. K., Badovinac, V. P. & Griffith, T. S. (2016). Clinical and Experimental Sepsis Impairs CD8 T-Cell-Mediated Immunity. Critical reviews in immunology, 36(1), 57-74. PMID: 27480902.

Cabrera-Perez, J., Babcock, J. C., Dileepan, T., Murphy, K. A., Kucaba, T. A., Badovinac, V. P. & Griffith, T. S. (2016). Gut Microbial Membership Modulates CD4 T Cell Reconstitution and Function after Sepsis. Journal of immunology, 197(5), 1692-8. PMID: 27448587.

Strother, R. K., Danahy, D. B., Kotov, D. I., Kucaba, T. A., Zacharias, Z. R., Griffith, T. S., Legge, K. L. & Badovinac, V. P. (2016). Polymicrobial Sepsis Diminishes Dendritic Cell Numbers and Function Directly Contributing to Impaired Primary CD8 T Cell Responses In Vivo. Journal of immunology, 197(11), 4301-4311. PMID: 27798171.

He, B., Xing, S., Chen, C., Gao, P., Teng, L., Shan, Q., Gullicksrud, J. A., Martin, M. D., Yu, S., Harty, J. T., Badovinac, V. P., Tan, K. & Xue, H. H. (2016). CD8<sup>+</sup> T Cells Utilize Highly Dynamic Enhancer Repertoires and Regulatory Circuitry in Response to Infections. Immunity, 45(6), 1341-1354. PMID: 27986453.

Martin, M. D., Kim, M. T., Shan, Q., Sompallae, R., Xue, H. H., Harty, J. T. & Badovinac, V. P. (2015). Phenotypic and Functional Alterations in Circulating Memory CD8 T Cells with Time after Primary Infection. PLoS pathogens, 11(10), e1005219. PMID: 26485703.

Khan, S. H., Badovinac, V. P. (2015). Listeria monocytogenes: a model pathogen to study antigen-specific memory CD8 T cell responses. Seminars in immunopathology, 37(3), 301-10. PMID: 25860798.

Cabrera-Perez, J., Condotta, S. A., James, B. R., Kashem, S. W., Brincks, E. L., Rai, D., Kucaba, T. A., Badovinac, V. P. & Griffith, T. S. (2015). Alterations in antigen-specific naive CD4 T cell precursors after sepsis impairs their responsiveness to pathogen challenge. Journal of immunology, 194(4), 1609-20. PMID: 25595784.

Kim, M. T., Richer, M. J., Gross, B. P., Norian, L. A., Badovinac, V. P. & Harty, J. T. (2015). Enhancing Dendritic Cell-based Immunotherapy with IL-2/Monoclonal Antibody Complexes for Control of Established Tumors. Journal of immunology, 195(9), 4537-44. PMID: 26408669.

Martin, M. D., Badovinac, V. P. (2015). Antigen-dependent and -independent contributions to primary memory CD8 T cell activation and protection following infection. Scientific reports, 5, 18022. PMID: 26658291.

Condotta, S. A., Khan, S. H., Rai, D., Griffith, T. S. & Badovinac, V. P. (2015). Polymicrobial Sepsis Increases Susceptibility to Chronic Viral Infection and Exacerbates CD8+ T Cell Exhaustion. Journal of immunology, 195(1), 116-25. PMID: 25980007.

Duong, S., Condotta, S. A., Rai, D., Martin, M. D., Griffith, T. S. & Badovinac, V. P. (2014). Polymicrobial Sepsis Alters Antigen-Dependent and -Independent Memory CD8 T Cell Functions. Journal of immunology, 192(8), 3618-25. PMID: 24646738.

Martin, M. D., Badovinac, V. P. (2014). Influence of time and number of antigen encounters on memory CD8 T cell development. Immunologic research, 59((1-3)), 35-44. PMID: 24825776.

Rai, D., Martin, M. D. & Badovinac, V. P. (2014). The longevity of memory CD8 T cell responses after repetitive antigen stimulations. Journal of immunology, 192(12), 5652-9. PMID: 24829415.

Cabrera-Perez, J., Condotta, S. A., Badovinac, V. P. & Griffith, T. S. (2014). Impact of sepsis on CD4 T cell immunity. Journal of leukocyte biology, 96(5), 767-77. PMID: 24791959.

Starbeck-Miller, G. R., Badovinac, V. P., Barber, D. L. & Harty, J. T. (2014). Cutting edge: Expression of Fc?RIIB tempers memory CD8 T cell function in vivo. Journal of Immunology, 192(1), 35-39. PMID: 24285839.

Markwart, R., Condotta, S. A., Requardt, R. P., Borken, F., Schubert, K., Weigel, C., Bauer, M., Griffith, T. S., Förster, M., Brunkhorst, F. M., Badovinac, V. P. & Rubio, I. (2014). Immunosuppression after sepsis: systemic inflammation and sepsis induce a loss of naïve T-cells but no enduring cell-autonomous defects in T-cell function. PLoS One, 9(12), e115094. PMID: 25541945.

Khan, S. H., Hemann, E. A., Legge, K. L., Norian, L. A. & Badovinac, V. P. (2014). Diet-induced obesity does not impact the generation and maintenance of primary memory CD8 T cells. Journal of Immunology, 193(12), 5873-82. PMID: 25378592.

Condotta, S. A., Rai, D., James, B. R., Griffith, T. S. & Badovinac, V. P. (2013). Sustained and incomplete recovery of naive CD8+ T cell precursors after sepsis contributes to impaired CD8+ T cell responses to infection. Journal of Immunology, 190(5), 1991-2000. PMID: 23355736.

Condotta, S. A., Cabrera-Perez, J., Badovinac, V. P. & Griffith, T. S. (2013). T-cell-mediated immunity and the role of TRAIL in sepsis-induced immunosuppression. Critical reviews in immunology, 33(1), 23-40. PMID: 23510024.

Pham, N. L., Badovinac, V. & Harty, J. T. (2012). Epitope specificity of memory CD8+ T cells dictates vaccination-induced mortality in LCMV-infected perforin-deficient mice. European journal of immunology, 42(6), 1488-99. PMID: 22678903.

Martin, M. D., Condotta, S. A., Harty, J. T. & Badovinac, V. (2012). Population dynamics of naive and memory CD8 T cell responses after antigen stimulations in vivo. Journal of immunology, 188(3), 1255-65. PMID: 22205031.

Condotta, S. A., Richer, M. J., Badovinac, V. & Harty, J. T. (2012). Probing CD8 T cell responses with Listeria monocytogenes infection. Advances in immunology, 113, 51-80. PMID: 22244579.

Nolz, J. C., Rai, D., Badovinac, V. & Harty, J. T. (2012). Division-linked generation of death-intermediates regulates the numerical stability of memory CD8 T cells. Proceedings of the National Academy of Sciences of the United States of America, 109(16), 6199-204. PMID: 22474367.

Martin, M. D., Wirth, T. C., Lauer, P., Harty, J. T. & Badovinac, V. (2011). The impact of pre-existing memory on differentiation of newly recruited naive CD8 T cells. Journal of immunology, 187(6), 2923-31. PMID: 21832161.

Wirth, T. C., Martin, M. D., Starbeck-Miller, G., Harty, J. T. & Badovinac, V. (2011). Secondary CD8+ T-cell responses are controlled by systemic inflammation. European journal of immunology, 41(5), 1321-33. PMID: 21425157.

Pham, N. L., Badovinac, V. & Harty, J. T. (2011). Differential role of "Signal 3" inflammatory cytokines in regulating CD8 T cell expansion and differentiation in vivo. Frontiers in immunology, 2, 4. PMID: 22566795.

Gurung, P., Rai, D., Condotta, S. A., Babcock, J. C., Badovinac, V. & Griffith, T. S. (2011). Immune unresponsiveness to secondary heterologous bacterial infection after sepsis induction is TRAIL dependent. Journal of immunology, 187(5), 2148-54. PMID: 21788440.

Schmidt, N. W., Butler, N. S., Badovinac, V. & Harty, J. T. (2010). Extreme CD8 T cell requirements for anti-malarial liver-stage immunity following immunization with radiation attenuated sporozoites. PLoS pathogens, 6(7), e1000998. PMID: 20657824.

Wirth, T. C., Harty, J. T. & Badovinac, V. (2010). Modulating numbers and phenotype of CD8+ T cells in secondary immune responses. European journal of immunology, 40(7), 1916-26. PMID: 20411564.

Schlub, T. E., Badovinac, V., Sabel, J. T., Harty, J. T. & Davenport, M. P. (2010). Predicting CD62L expression during the CD8+ T-cell response in vivo. Immunology and cell biology, 88(2), 157-64. PMID: 19859082.

Zhou, X., Yu, S., Zhao, D. M., Harty, J. T., Badovinac, V. & Xue, H. H. (2010). Differentiation and persistence of memory CD8(+) T cells depend on T cell factor 1. Immunity, 33(2), 229-40. PMID: 20727791.

Wirth, T. C., Xue, H. H., Rai, D., Sabel, J. T., Bair, T., Harty, J. T. & Badovinac, V. (2010). Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8(+) T cell differentiation. Immunity, 33(1), 128-40. PMID: 20619696.

Zhao, D. M., Yu, S., Zhou, X., Haring, J. S., Held, W., Badovinac, V., Harty, J. T. & Xue, H. H. (2010). Constitutive activation of Wnt signaling favors generation of memory CD8 T cells. Journal of immunology, 184(3), 1191-9. PMID: 20026746.

Pham, N. L., Pewe, L. L., Fleenor, C. J., Langlois, R. A., Legge, K. L., Badovinac, V. & Harty, J. T. (2010). Exploiting cross-priming to generate protective CD8 T-cell immunity rapidly. Proceedings of the National Academy of Sciences of the United States of America, 107(27), 12198-203. PMID: 20616089.

Rai, D., Pham, N. L., Harty, J. T. & Badovinac, V. (2009). Tracking the total CD8 T cell response to infection reveals substantial discordance in magnitude and kinetics between inbred and outbred hosts. Journal of immunology, 183(12), 7672-81. PMID: 19933864.

Pham, N. L., Badovinac, V. & Harty, J. T. (2009). A default pathway of memory CD8 T cell differentiation after dendritic cell immunization is deflected by encounter with inflammatory cytokines during antigen-driven proliferation. Journal of immunology, 183(4), 2337-48. PMID: 19635915.

Wirth, T. C., Pham, N. L., Harty, J. T. & Badovinac, V. (2009). High initial frequency of TCR-transgenic CD8 T cells alters inflammation and pathogen clearance without affecting memory T cell function. Molecular immunology, 47(1), 71-8. PMID: 19195704.

Wirth, T. C., Badovinac, V., Zhao, L., Dailey, M. O. & Harty, J. T. (2009). Differentiation of central memory CD8 T cells is independent of CD62L-mediated trafficking to lymph nodes. Journal of immunology, 182(10), 6195-206. PMID: 19414773.

Harty, J. T., Badovinac, V. (2008). Shaping and reshaping CD8+ T-cell memory. Nature reviews. Immunology, 8(2), 107-19. PMID: 18219309.

Badovinac, V., Schmidt, N. W., Podyminogin, R. L., Butler, N. S., Badovinac, V. P., Tucker, B. J., Bahjat, K. S., Lauer, P., Reyes-Sandoval, A., Hutchings, C. L., Moore, A. C., Gilbert, S. C., Hill, A. V., Bartholomay, L. C. & Harty, J. T. (2008). Memory CD8 T cell responses exceeding a large but definable threshold provide long-term immunity to malaria. Proceedings of the National Academy of Sciences of the United States of America, 105(37), 14017-22. PMID: 18780790.

Humphreys, T. D., Khanolkar, A., Badovinac, V. & Harty, J. T. (2008). Generation and maintenance of Listeria-specific CD8+ T cell responses in perforin-deficient mice chronically infected with LCMV. Virology, 370(2), 310-22. PMID: 17936870.

Stojakovic, M., Salazar-Fontana, L. I., Tatari-Calderone, Z., Badovinac, V., Santori, F. R., Kovalovsky, D., Sant'Angelo, D., Harty, J. T. & Vukmanovic, S. (2008). Adaptable TCR avidity thresholds for negative selection. Journal of immunology, 181(10), 6770-8. PMID: 18981094.

Khanolkar, A., Badovinac, V. & Harty, J. T. (2007). CD8 T cell memory development: CD4 T cell help is appreciated. Immunologic research, 39(3-Jan), 94-104. PMID: 17917058.

Santori, F. R., Popmihajlov, Z., Badovinac, V., Smith, C., Radoja, S., Harty, J. T. & Vukmanovic, S. (2007). TCR beta chain that forms peptide-independent alloreactive TCR transfers reduced reactivity with irrelevant peptide/MHC complex. Journal of immunology, 178(10), 6109-14. PMID: 17475836.

Messingham, K. A., Badovinac, V., Jabbari, A. & Harty, J. T. (2007). A role for IFN-gamma from antigen-specific CD8+ T cells in protective immunity to Listeria monocytogenes. Journal of immunology (Baltimore, Md. : 1950), 179(4), 2457-66. PMID: 17675507.

Badovinac, V., Haring, J. S. & Harty, J. T. (2007). Initial T cell receptor transgenic cell precursor frequency dictates critical aspects of the CD8(+) T cell response to infection. Immunity, 26(6), 827-41. PMID: 17555991.

Badovinac, V., Harty, J. T. (2007). Manipulating the rate of memory CD8+ T cell generation after acute infection. Journal of immunology, 179(1), 53-63. PMID: 17579021.

Hamilton, S. E., Badovinac, V., Khanolkar, A. & Harty, J. T. (2006). Listeriolysin O-deficient Listeria monocytogenes as a vaccine delivery vehicle: antigen-specific CD8 T cell priming and protective immunity. Journal of immunology, 177(6), 4012-20. PMID: 16951364.

Badovinac, V., Harty, J. T. (2006). Programming, demarcating, and manipulating CD8+ T-cell memory. Immunological reviews, 211, 67-80. PMID: 16824118.

Haring, J. S., Badovinac, V. & Harty, J. T. (2006). Inflaming the CD8+ T cell response. Immunity, 25(1), 19-29. PMID: 16860754.

Badovinac, V., Messingham, K. A., Griffith, T. S. & Harty, J. T. (2006). TRAIL deficiency delays, but does not prevent, erosion in the quality of "helpless" memory CD8 T cells. Journal of immunology, 177(2), 999-1006. PMID: 16818756.

Badovinac, V., Messingham, K. A., Jabbari, A., Haring, J. S. & Harty, J. T. (2005). Accelerated CD8+ T-cell memory and prime-boost response after dendritic-cell vaccination. Nature medicine, 11(7), 748-56. PMID: 15951824.

Haring, J. S., Badovinac, V., Olson, M. R., Varga, S. M. & Harty, J. T. (2005). In vivo generation of pathogen-specific Th1 cells in the absence of the IFN-gamma receptor. Journal of immunology, 175(5), 3117-22. PMID: 16116201.

Hamilton, S. E., Porter, B. B., Messingham, K. A., Badovinac, V. & Harty, J. T. (2004). MHC class Ia-restricted memory T cells inhibit expansion of a nonprotective MHC class Ib (H2-M3)-restricted memory response. Nature immunology, 5(2), 159-68. PMID: 14745446.

Badovinac, V., Porter, B. B. & Harty, J. T. (2004). CD8+ T cell contraction is controlled by early inflammation. Nature immunology, 5(8), 809-17. PMID: 15247915.

Messingham, K. A., Badovinac, V. & Harty, J. T. (2003). Deficient anti-listerial immunity in the absence of perforin can be restored by increasing memory CD8+ T cell numbers. Journal of immunology, 171(8), 4254-62. PMID: 14530349.

Badovinac, V., Hamilton, S. E. & Harty, J. T. (2003). Viral infection results in massive CD8+ T cell expansion and mortality in vaccinated perforin-deficient mice. Immunity, 18(4), 463-74. PMID: 12705850.

Badovinac, V., Harty, J. T. (2003). Memory lanes. Nature immunology, 4(3), 212-3. PMID: 12605228.

Badovinac, V., Messingham, K. A., Hamilton, S. E. & Harty, J. T. (2003). Regulation of CD8+ T cells undergoing primary and secondary responses to infection in the same host. Journal of immunology, 170(10), 4933-42. PMID: 12734336.

Badovinac, V., Porter, B. B. & Harty, J. T. (2002). Programmed contraction of CD8(+) T cells after infection. Nature immunology, 3(7), 619-26. PMID: 12055624.

Badovinac, V., Harty, J. T. (2002). CD8(+) T-cell homeostasis after infection: setting the 'curve'. Microbes and infection, 4(4), 441-7. PMID: 11932195.

Harty, J. T., Badovinac, V. (2002). Influence of effector molecules on the CD8(+) T cell response to infection. Current opinion in immunology, 14(3), 360-5. PMID: 11973135.

Badovinac, V., Harty, J. T. (2001). Detection and analysis of antigen-specific CD8+ T cells. Immunologic research, 24(3), 325-32. PMID: 11817329.

Stosic-Grujicic, S., Maksimovic, D., Badovinac, V., Samardzic, T., Trajkovic, V., Lukic, M. & Mostarica Stojkovic, M. (2001). Antidiabetogenic effect of pentoxifylline is associated with systemic and target tissue modulation of cytokines and nitric oxide production. Journal of autoimmunity, 16(1), 47-58. PMID: 11221996.

Badovinac, V., Tvinnereim, A. R. & Harty, J. T. (2000). Regulation of antigen-specific CD8+ T cell homeostasis by perforin and interferon-gamma. Science, 290(5495), 1354-8. PMID: 11082062.

White, D. W., Badovinac, V., Fan, X. & Harty, J. T. (2000). Adaptive immunity against Listeria monocytogenes in the absence of type I tumor necrosis factor receptor p55. Infection and immunity, 68(8), 4470-6. PMID: 10899844.

Badovinac, V., Harty, J. T. (2000). Intracellular staining for TNF and IFN-gamma detects different frequencies of antigen-specific CD8(+) T cells. Journal of immunological methods, 238(2-Jan), 107-17. PMID: 10758241.

Badovinac, V., Corbin, G. A. & Harty, J. T. (2000). Cutting edge: OFF cycling of TNF production by antigen-specific CD8+ T cells is antigen independent. Journal of immunology, 165(10), 5387-91. PMID: 11067887.

Trajkovic, V., Stepanovic, S., Samardzic, T., Jankovic, V., Badovinac, V. & Mostarica Stojkovic, M. (2000). Cryptococcus neoformans neutralizes macrophage and astrocyte derived nitric oxide without interfering with inducible nitric oxide synthase induction or catalytic activity - possible involvement of nitric oxide consumption. Scandinavian journal of immunology, 51(4), 384-91. PMID: 10736111.

Badovinac, V., Harty, J. T. (2000). Adaptive immunity and enhanced CD8+ T cell response to Listeria monocytogenes in the absence of perforin and IFN-gamma. Journal of immunology, 164(12), 6444-52. PMID: 10843700.

White, D. W., Badovinac, V., Kollias, G. & Harty, J. T. (2000). Cutting edge: antilisterial activity of CD8+ T cells derived from TNF-deficient and TNF/perforin double-deficient mice. Journal of immunology, 165(1), 5-9. PMID: 10861027.

Badovinac, V., Trajkovic, V. & Mostarica-Stojkovic, M. (2000). Nitric oxide promotes growth and major histocompatibility complex-unrestricted cytotoxicity of interleukin-2-activated rat lymphocytesCutting edge: antilisterial act. Scandinavian journal of immunology, 52(1), 62-70. PMID: 10886785.

Trajkovic, V., Badovinac, V., Jankovic, V. & Mostarica Stojkovic, M. (1999). Cyclosporin A inhibits activation of inducible nitric oxide synthase in C6 glioma cell line. Brain research, 816(1), 92-8. PMID: 9878697.

Trajkovic, V., Badovinac, V., Jankovic, V., Samardzic, T., Maksimovic, D. & Popadic, D. (1999). Cyclosporin A suppresses the induction of nitric oxide synthesis in interferon-gamma-treated L929 fibroblasts. Scandinavian journal of immunology, 49(2), 126-30. PMID: 10075015.

Badovinac, V., Mostarica-Stojkovic, M., Dinarello, C. A. & Stosic-Grujicic, S. (1998). Interleukin-1 receptor antagonist suppresses experimental autoimmune encephalomyelitis (EAE) in rats by influencing the activation and proliferation of encephalitogenic cells. Journal of neuroimmunology, 85(1), 87-95. PMID: 9627001.

Stosic-Grujicic, S., Trajkovic, V., Badovinac, V. & Mostarica Stojkovic, M. (1998). Pentoxifylline potentiates nitric oxide production and growth suppression in interferon-gamma-treated L929 fibroblasts. Cellular immunology, 184(2), 105-11. PMID: 9630836.

Badovinac, V., Boggiano, C., Trajkovic, V., Frey, A. B., Vujanovic, N. L., Gold, D. P., Mostarica-Stojkovic, M. & Vukmanovic, S. (1998). Rat NKR-P1+ CD3+ T cells: selective proliferation in interleukin-2, diverse T-cell-receptor-Vbeta repertoire and polarized interferon-gamma expression. Immunology, 95(1), 117-25. PMID: 9767466.

Trajkovic, V., Badovinac, V., Popadic, D., Hadzic, O. & Stojkovic, M. M. (1997). Cell-specific effects of pentoxifylline on nitric oxide production and inducible nitric oxide synthase mRNA expression. Immunology, 92(3), 402-6. PMID: 9486115.

Badovinac, V., Pravica, V., Trajkovic, V., Kraguljac, N., Ejdus, L. & Mostarica-Stojkovic, M. (1996). Correlation between number of NK (NKR-P1+) cells and production of cytokines in inbred AO and DA rats. Mikrobiologija, 33, 27-33.

Arsov, I., Pravica, V., Ejdus, L., Badovinac, V., Mostarica, M. & Lukic, M. L. (1995). Selection for susceptibility to experimental allergic encephalomyelitis also selects for high IFN-gamma production. Transplantation proceedings, 27(2), 1537-8. PMID: 7725403.