Ashutosh Mangalam, PhD

Portrait
Assistant Professor of Pathology

Contact Information

Primary Office
1080-A Medical Laboratories
Iowa City, IA 52242
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319-335-8558

Education

BS, Biology, Lucknow University, Lucknow, India
MS, Biotechnology, Devi Ahilya University, Indore, India
PhD, Immunology, Sanjay Gandhi Post Graduate Institute of Medical Sciences
Postdoctoral Fellow, Department of Immunology, Mayo Clinic, Rochester, MN

Education/Training Program Affiliations

Interdisciplinary Graduate Program in Immunology, Interdisciplinary Graduate Program in Molecular Medicine

Center, Program and Institute Affiliations

Center for Immunology and Immune-based Diseases

Research Summary

The major goal of my research is to develop better treatment options to cure multiple sclerosis (MS). To achieve this goal, my research program focuses on three major interconnected themes: i) utilizing transgenic mice expressing MS susceptible and resistant HLA class II molecules (HLA transgenic mice) to understand the mechanisms of disease pathogenesis; ii) to understand the role of gut microbiome and metabolome in the etiopathogenesis of MS; and iii) test therapeutic efficacy of human gut derived bacteria as drug (BRUG) in animal models of MS.

Publications

Marietta, E., Mangalam, A., Murray, J. & Taneja, V. (2018). Reply. (Vols. 70). (2), pp. 321-322. Arthritis & rheumatology (Hoboken, N.J.). PMID: 28950432.

Freedman, S. N., Shahi, S. K. & Mangalam, A. K. (2018). The "Gut Feeling": Breaking Down the Role of Gut Microbiome in Multiple Sclerosis. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 15(1), 109-125. PMID: 29204955.

Mangalam, A., Shahi, S. K., Luckey, D., Karau, M., Marietta, E., Luo, N., Choung, R. S., Ju, J., Sompallae, R., Gibson-Corley, K., Patel, R., Rodriguez, M., David, C., Taneja, V. & Murray, J. (2017). Human Gut-Derived Commensal Bacteria Suppress CNS Inflammatory and Demyelinating Disease. Cell reports, 20(6), 1269-1277. PMID: 28793252.

Zhao, J., Alshukairi, A. N., Baharoon, S. A., Ahmed, W. A., Bokhari, A. A., Nehdi, A. M., Layqah, L. A., Alghamdi, M. G., Al Gethamy, M. M., Dada, A. M., Khalid, I., Boujelal, M., Al Johani, S. M., Vogel, L., Subbarao, K., Mangalam, A., Wu, C., Ten Eyck, P., Perlman, S. & Zhao, J. (2017). Recovery from the Middle East respiratory syndrome is associated with antibody and T-cell responses. Science immunology, 2(14). PMID: 28778905.

Mangalam, A. K., Rattan, R., Suhail, H., Singh, J., Hoda, M. N., Deshpande, M., Fulzele, S., Denic, A., Shridhar, V., Kumar, A., Viollet, B., Rodriguez, M. & Giri, S. (2016). AMP-Activated Protein Kinase Suppresses Autoimmune Central Nervous System Disease by Regulating M1-Type Macrophage-Th17 Axis. Journal of immunology (Baltimore, Md. : 1950), 197(3), 747-60. PMID: 27354217.

Chen, J., Chia, N., Kalari, K. R., Yao, J. Z., Novotna, M., Soldan, M. M., Luckey, D. H., Marietta, E. V., Jeraldo, P. R., Chen, X., Weinshenker, B. G., Rodriguez, M., Kantarci, O. H., Nelson, H., Murray, J. A. & Mangalam, A. K. (2016). Multiple sclerosis patients have a distinct gut microbiota compared to healthy controls. Scientific reports, 6, 28484. PMID: 27346372.

Marietta, E. V., Murray, J. A., Luckey, D. H., Jeraldo, P. R., Lamba, A., Patel, R., Luthra, H. S., Mangalam, A. & Taneja, V. (2016). Human Gut-Derived Prevotella histicola Suppresses Inflammatory Arthritis in Humanized Mice. Arthritis & rheumatology (Hoboken, N.J.). PMID: 27337150.

Zhao, J., Zhao, J., Mangalam, A. K., Channappanavar, R., Fett, C., Meyerholz, D. K., Agnihothram, S., Baric, R. S., David, C. S. & Perlman, S. (2016). Airway Memory CD4(+) T Cells Mediate Protective Immunity against Emerging Respiratory Coronaviruses. Immunity, 44(6), 1379-91. PMID: 27287409.

Poisson, L. M., Suhail, H., Singh, J., Datta, I., Denic, A., Labuzek, K., Hoda, M. N., Shankar, A., Kumar, A., Cerghet, M., Elias, S., Mohney, R. P., Rodriguez, M., Rattan, R., Mangalam, A. K. & Giri, S. (2015). Untargeted Plasma Metabolomics Identifies Endogenous Metabolite with Drug-like Properties in Chronic Animal Model of Multiple Sclerosis. The Journal of biological chemistry, 290(52), 30697-712. PMID: 26546682.

Blanco, D. B., Luckey, D., Smart, M., Pittelkow, M., Kumar, R., David, C. S. & Mangalam, A. K. (2014). Modulation of Immune response by Ultra-violet light in HLA class-II transgenic mice. Jacobs journal of allergy and immunology, 1(1). PMID: 26473171.

Mangalam, A. K., Luo, N., Luckey, D., Papke, L., Hubbard, A., Wussow, A., Smart, M., Giri, S., Rodriguez, M. & David, C. (2014). Absence of IFN-? increases brain pathology in experimental autoimmune encephalomyelitis-susceptible DRB1*0301.DQ8 HLA transgenic mice through secretion of proinflammatory cytokine IL-17 and induction of pathogenic monocytes/microglia into the central nervous system. Journal of immunology, 193(10), 4859-70. PMID: 25339670.

Jeraldo, P., Kalari, K., Chen, X., Bhavsar, J., Mangalam, A., White, B., Nelson, H., Kocher, J. P. & Chia, N. (2014). IM-TORNADO: a tool for comparison of 16S reads from paired-end libraries. PloS one, 9(12), e114804. PMID: 25506826.

Mangalam, A. K., Taneja, V. & David, C. S. (2013). HLA class II molecules influence susceptibility versus protection in inflammatory diseases by determining the cytokine profile. Journal of immunology, 190(2), 513-8. PMID: 23293357.

Mangalam, A., Poisson, L., Nemutlu, E., Datta, I., Denic, A., Dzeja, P., Rodriguez, M., Rattan, R. & Giri, S. (2013). Profile of Circulatory Metabolites in a Relapsing-remitting Animal Model of Multiple Sclerosis using Global Metabolomics. Journal of clinical & cellular immunology, 4. PMID: 24273690.

Piotrowski, J. T., Gomez, T. S., Schoon, R. A., Mangalam, A. K. & Billadeau, D. D. (2013). WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function. Molecular and cellular biology, 33(5), 958-73. PMID: 23275443.

Mangalam, A. K., Luckey, D., Giri, S., Smart, M., Pease, L. R., Rodriguez, M. & David, C. S. (2012). Two discreet subsets of CD8 T cells modulate PLP(91-110) induced experimental autoimmune encephalomyelitis in HLA-DR3 transgenic mice. Journal of autoimmunity, 38(4), 344-53. PMID: 22459490.

Luckey, D., Bastakoty, D. & Mangalam, A. K. (2011). Role of HLA class II genes in susceptibility and resistance to multiple sclerosis: studies using HLA transgenic mice. Journal of autoimmunity, 37(2), 122-8. PMID: 21632210.

Mangalam, A., Luckey, D., Basal, E., Jackson, M., Smart, M., Rodriguez, M. & David, C. (2009). HLA-DQ8 (DQB1*0302)-restricted Th17 cells exacerbate experimental autoimmune encephalomyelitis in HLA-DR3-transgenic mice. Journal of immunology, 182(8), 5131-9. PMID: 19342694.

Nath, N., Khan, M., Rattan, R., Mangalam, A., Makkar, R. S., de Meester, C., Bertrand, L., Singh, I., Chen, Y., Viollet, B. & Giri, S. (2009). Loss of AMPK exacerbates experimental autoimmune encephalomyelitis disease severity. Biochemical and biophysical research communications, 386(1), 16-20. PMID: 19486896.

Mangalam, A. K., Rajagopalan, G., Taneja, V. & David, C. S. (2008). HLA class II transgenic mice mimic human inflammatory diseases. Advances in immunology, 97, 65-147. PMID: 18501769.

Mangalam, A., Luckey, D., Basal, E., Behrens, M., Rodriguez, M. & David, C. (2008). HLA-DQ6 (DQB1*0601)-restricted T cells protect against experimental autoimmune encephalomyelitis in HLA-DR3.DQ6 double-transgenic mice by generating anti-inflammatory IFN-gamma. Journal of immunology, 180(11), 7747-56. PMID: 18490779.

Mangalam, A., Rodriguez, M. & David, C. (2007). A new humanized HLA transgenic mouse model of multiple sclerosis expressing class II on mouse CD4 T cells. Annals of the New York Academy of Sciences, 1103, 112-7. PMID: 17376829.

Rajagopalan, G., Mangalam, A. K., Sen, M. M., Cheng, S., Kudva, Y. C. & David, C. S. (2007). Autoimmunity in HLA-DQ8 transgenic mice expressing granulocyte/macrophage-colony stimulating factor in the beta cells of islets of Langerhans. Autoimmunity, 40(3), 169-79. PMID: 17453715.

Rajagopalan, G., Mangalam, A. K., Sen, M. M., Kudva, Y. C. & David, C. S. (2007). Distinct local immunogenic stimuli dictate differential requirements for CD4+ and CD8+ T cell subsets in the pathogenesis of spontaneous autoimmune diabetes. Autoimmunity, 40(7), 489-96. PMID: 17966038.

Taneja, V., Behrens, M., Mangalam, A., Griffiths, M. M., Luthra, H. S. & David, C. S. (2007). New humanized HLA-DR4-transgenic mice that mimic the sex bias of rheumatoid arthritis. Arthritis and rheumatism, 56(1), 69-78. PMID: 17195209.

Mangalam, A. K., Rodriguez, M. & David, C. S. (2006). HLA class-II transgenic mice expressing class-II on subset of CD4+ T cells as a model of human MS. 8th International Congress of Neuroimmunology, 101-105.

Mangalam, A., Rodriguez, M. & David, C. (2006). Role of MHC class II expressing CD4+ T cells in proteolipid protein(91-110)-induced EAE in HLA-DR3 transgenic mice. European journal of immunology, 36(12), 3356-70. PMID: 17125142.

Mangalam, A. K., Khare, M., Krco, C. J., Rodriguez, M. & David, C. S. (2005). Delineation of the minimal encephalitogenic epitope of proteolipid protein peptide(91-110) and critical residues required for induction of EAE in HLA-DR3 transgenic mice. Journal of neuroimmunology, 161(1-2), 40-8. PMID: 15748942.

Khare, M., Mangalam, A., Rodriguez, M. & David, C. S. (2005). HLA DR and DQ interaction in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis in HLA class II transgenic mice. Journal of neuroimmunology, 169(1-2), 1-12. PMID: 16194572.

Mangalam, A. K., Khare, M., Krco, C., Rodriguez, M. & David, C. (2004). Identification of T cell epitopes on human proteolipid protein and induction of experimental autoimmune encephalomyelitis in HLA class II-transgenic mice. European journal of immunology, 34(1), 280-90. PMID: 14971054.

Mangalam, A. K., Aggarwal, A. & Naik, S. (2002). Gold sodium thiomalate (GSTM) inhibits lipopolysaccharide stimulated tumor necrosis factor-alpha through ceramide pathway. Cellular immunology, 219(1), 1-10. PMID: 12473262.

Mangalam, A. K., Aggarwal, A. & Naik, S. (2001). Mechanism of action of disease modifying anti-rheumatic agent, gold sodium thiomalate (GSTM). International immunopharmacology, 1(6), 1165-72. PMID: 11407310.

Singh, V. K., Mangalam, A. K., Kumar, A. & Naik, S. (2001). Universal primers can amplify tumor necrosis factor gene across species. Molecular biology today, 2(1), 11-12.

Singh, V. K., Mangalam, A. K., Dwivedi, S. & Naik, S. (1998). Primer premier: program for design of degenerate primers from a protein sequence. BioTechniques, 24(2), 318-9. PMID: 9494736.