Adam Dupuy, PhD

Portrait
Associate Professor of Anatomy and Cell Biology
Associate Professor of Pathology

Contact Information

Primary Office: 3202 MERF
Iowa City, IA 52242
319-335-8090

Education

BA, Biology and Chemistry, Central College
PhD, Molecular, Cellular, Developmental Biology, and Genetics, University of Minnesota

Postdoctoral Fellow, University of Minnesota Cancer Center
Postdoctoral Fellow, Mouse Cancer Genetics, National Cancer Institute

Education/Training Program Affiliations

Department of Cell and Developmental Graduate Program, Interdisciplinary Graduate Program in Genetics, Interdisciplinary Graduate Program in Molecular Medicine, Medical Scientist Training Program

Research Summary

The goal of my current research is to understand the role that somatic mutation plays in all aspects of tumor biology. Much of our work has made use of the Sleeping Beauty (SB) transposon system to engineer mouse cancer models in which somatic mutations are generated by transposon insertions. We and our collaborators have recently described a variety of tumor models using the approach, and these studies have identified many novel candidate cancer genes. The current work in my laboratory focuses on the genetics of hepatocellular carcinoma and T-cell leukemia. In addition, we have ongoing collaborative efforts to develop SB models of ovarian and endometrial cancer. Future work in the lab will focus on using our mouse models to address relevant questions encountered by oncologists in the clinic (e.g. metastasis, drug resistance).

Publications

Ziyad, S., Riordan, J. D., Cavanaugh, A. M., Su, T., Hernandez, G. E., Hilfenhaus, G., Morselli, M., Huynh, K., Wang, K., Chen, J. N., Dupuy, A. J. & Iruela-Arispe, M. L. (2018). A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis. Cell reports, 22(5), 1211-1224. PMID: 29386109.

Suárez-Cabrera, C., Quintana, R. M., Bravo, A., Casanova, M. L., Page, A., Alameda, J. P., Paramio, J. M., Maroto, A., Salamanca, J., Dupuy, A. J., Ramírez, A. & Navarro, M. (2017). A transposon-based analysis reveals RASA1 is involved in triple negative breast cancer. Cancer Research, 77(6), 1357-1368. PMID: 28108518.

Rauckhorst, A. J., Gray, L. R., Sheldon, R. D., Fu, X., Pewa, A. D., Feddersen, C. R., Dupuy, A. J., Gibson-Corley, K. N., Cox, J. E., Burgess, S. C. & Taylor, E. B. (2017). The mitochondrial pyruvate carrier mediates high fat diet-induced increases in hepatic TCA cycle capacity. Molecular Metabolism, 6(11), 1468-1479. PMID: 29107293.

Temiz, N. A., Moriarity, B. S., Wolf, N. K., Riordan, J. D., Dupuy, A. J., Largaespada, D. A. & Sarver, A. L. (2016). RNA sequencing of Sleeping Beauty transposon-induced tumors detects transposon-RNA fusions in forward genetic cancer screens. Genome Research, 26(1), 119-129. PMID: 26553456.

Guimarães-Young, A., Neff, T., Dupuy, A. J. & Goodheart, M. J. (2016). Conditional deletion of Sox17 reveals complex effects on uterine adenogenesis and function. Developmental Biology, 414(2), 219-227. PMID: 27102016.

Thiel, W. H., Thiel, K. W., Flenker, K. S., Bair, T., Dupuy, A. J., McNamara, 2nd, J. O., Miller, F. J. & Giangrande, P. H. (2015). Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells. Methods in molecular biology, 1218, 187-99. PMID: 25319652.

Smith, R. P., Riordan, J. D., Feddersen, C. R. & Dupuy, A. J. (2015). A Hybrid Adenoviral Vector System Achieves Efficient Long-Term Gene Expression in the Liver via piggyBac Transposition. Human gene therapy, 26(6), 377-85. PMID: 25808258.

Gibson-Corley, K. N., Rogers, L. M., Goeken, A., Dupuy, A. J. & Meyerholz, D. K. (2014). Keratoacanthoma Pathobiology in Mouse Models. Diseases, 2(2), 106-119. PMID: 25309748.

Zhang, Z., Kim, K., Li, X., Moreno, M., Sharp, T., Goodheart, M. J., Safe, S., Dupuy, A. J. & Amendt, B. A. (2014). MicroRNA-26b represses colon cancer cell proliferation by inhibiting lymphoid enhancer factor 1 expression. Molecular Cancer Therapeutics(American Association for Cancer Research), 13(7), 1942-1951. PMID: 24785257.

Riordan, J. D., Drury L, L. J., Smith, R. P., Brett, B. T., Rogers, L. M., Scheetz, T. E. & Dupuy, A. J. (2014). Sequencing methods and datasets to improve functional interpretation of sleeping beauty mutagenesis screens. BMC Geonmics, 15, 1150. DOI: 10.1186/1471-2164-15-1150.

Rogers, L. M., Olivier, A. K., Meyerholz, D. K. & Dupuy, A. J. (2013). Adaptive immunity does not strongly suppress spontaneous tumors in a Sleeping Beauty model of cancer. Journal of immunology (Baltimore, Md. : 1950), 190(8), 4393-9. PMID: 23475219.

Riordan, J. D., Keng, V. W., Tschida, B. R., Scheetz, T. E., Bell, J. B., Podetz-Pedersen, K. M., Moser, C. D., Copeland, N. G., Jenkins, N. A., Roberts, L. R., Largaespada, D. A. & Dupuy, A. J. (2013). Identification of rtl1, a retrotransposon-derived imprinted gene, as a novel driver of hepatocarcinogenesis. PLoS genetics, 9(4), e1003441. PMID: 23593033.

Rogers, L. M., Riordan, J. D., Swick, B. L., Meyerholz, D. K. & Dupuy, A. J. (2013). Ectopic Expression of Zmiz1 Induces Cutaneous Squamous Cell Malignancies in a Mouse Model of Cancer. The Journal of investigative dermatology. PMID: 23426136.

Berquam-Vrieze, K. E., Swing, D. A., Tessarollo, L. & Dupuy, A. J. (2012). Characterization of transgenic mice expressing cancer-associated variants of human NOTCH1. Genesis (New York, N.Y. : 2000), 50(2), 112-8. PMID: 21898766.

Brett, B. T., Berquam-Vrieze, K. E., Nannapaneni, K., Huang, J., Scheetz, T. E. & Dupuy, A. J. (2011). Novel molecular and computational methods improve the accuracy of insertion site analysis in Sleeping Beauty-induced tumors. PloS one, 6(9), e24668. PMID: 21931803.

McGrail, M., Hatler, J. M., Kuang, X., Liao, H. K., Nannapaneni, K., Watt, K. E., Uhl, J. D., Largaespada, D. A., Vollbrecht, E., Scheetz, T. E., Dupuy, A. J., Hostetter, J. M. & Essner, J. J. (2011). Somatic mutagenesis with a Sleeping Beauty transposon system leads to solid tumor formation in zebrafish. PloS one, 6(4), e18826. PMID: 21533036.

Thiel, W. H., Bair, T., Wyatt Thiel, K., Dassie, J. P., Rockey, W. M., Howell, C. A., Liu, X. Y., Dupuy, A. J., Huang, L., Owczarzy, R., Behlke, M. A., McNamara, J. O. & Giangrande, P. H. (2011). Nucleotide bias observed with a short SELEX RNA aptamer library. Nucleic acid therapeutics, 21(4), 253-63. PMID: 21793789.

Berquam-Vrieze, K. E., Nannapaneni, K., Brett, B. T., Holmfeldt, L., Ma, J., Zagorodna, O., Jenkins, N. A., Copeland, N. G., Meyerholz, D. K., Knudson, C. M., Mullighan, C. G., Scheetz, T. E. & Dupuy, A. J. (2011). Cell of origin strongly influences genetic selection in a mouse model of T-ALL. Blood, 118(17), 4646-56. PMID: 21828136.

Dupuy, A. J. (2010). Current applications of transposons in mouse genetics. Methods in enzymology, 477, 53-70. PMID: 20699136.

Dupuy, A. J. (2010). Transposon-based screens for cancer gene discovery in mouse models. Seminars in cancer biology, 20(4), 261-8. PMID: 20478384.

Dupuy, A. J., Rogers, L. M., Kim, J., Nannapaneni, K., Starr, T. K., Liu, P., Largaespada, D. A., Scheetz, T. E., Jenkins, N. A. & Copeland, N. G. (2009). A modified sleeping beauty transposon system that can be used to model a wide variety of human cancers in mice. Cancer research, 69(20), 8150-6. PMID: 19808965.