LRS Cones, a Source of Viable White Blood Cells

In compliance with regulations, the DeGowin Blood Center routinely uses leukocyte depletion filters, also called Leukocyte Reduction System (LRS) cones, to eliminate the contaminating leukocytes from blood products, such as red blood cells, at the point of collection. These LRS cones are a source of viable human white blood cells that can be used in research applications. Below is a list of links to articles describing the characteristics and use of the cells obtained from the leukocyte reduction filters.

Please contact Barb Smith at (319) 356-2058 with technical questions regarding the LRS cones.

Articles:

Boudreau G, Carli C, Lamarche C, et al.  Leukoreduction system chambers are a reliable cellular source for the manufacturing of T-cell therapeutics. PDF Transfusion. Apr. 2019;59,1300-1311.

Cook MA, Jobson SE, Atkinson DC, et al. Used Leucodepletion Filters as a Source of Large Quantities of DNA Suitable for the Study of Genetic Variations in Human Populations. PDF Transfusion Medicine. 2003; 13:77-81.

Dietz AB, Bulur PA, Emery RL, et al.  A Novel Source of Viable Peripheral Blood Mononuclear Cells from Leukoreduction System Chambers. PDF Transfusion. Dec. 2006; 46:2083-2089.

Ebner S, Neyer S, Hofer S, Nussbaumer W, Romani N, Heufler C. Generation of Large Numbers of Human Dendritic Cells from Whole Blood Passaged Through Leukocyte Removal Filters: An Alternative to Standard Buffy Coats. PDF Journal of Immunological Methods. 2001; 252:93-104.

Ivanovic Z, Duchez P, Morgan D, et al. Whole-blood Leukodepletion Filters as a Source of CD34+ Progenitors Potentially Usable in Cell Therapy. PDF Transfusion. Jan. 2006; 46:118-125. 

Meyer TPH, Zehnter B, Hofmann B, et al. Filter Buffy Coats (FBC): A Source of Peripheral Blood Leukocytes Recovered from Leukocyte Depletion Filters. PDF Journal of Immunological Methods. 2005; 307:150-166. 

Néron S, Thibault L, Dussault N, Côté G, et al. Characterization of Mononuclear Cells Remaining in the Leukoreduction System Chambers of Apheresis Instruments after Routine Platelet Collection: A New Source of Viable Human Blood Cells. PDF Transfusion. June 2007; 47: 1042-1049. 

Néron S, Dussault N, Racine C. Whole-blood Leukoreduction Filters are a Source for Cryopreserved Cells for Phenotypic and Functional Investigations on Peripheral Blood Lymphocytes. PDF Transfusion. Apr. 2006;46:537-544. 

Norden, Ida Karin. Leukoreduction System Chambers Provide a Valuable Source of Functional Monocytes for the Monocyte Activation Test by Comparison with Internationally Validated Methods. PDF Journal of Immunological Methods. 2016; 428:42–49.

Peytour Y, Villacreces A, Chevaleyre J, et al. Obtaining of CD34+ Cells from Healthy Blood Donors: Development of a Rapid and Efficient Procedure using Leukoreduction Filters. PDF Transfusion. Oct. 2010;50:2152-2157.

Peytour Y, Villacreces A, Chevaleyre J, et al. Discarded Leukoreduction Filters: A New Source of Stem Cells for Research, Cell Engineering and Therapy? PDF Stem Cell Research (2013), 11, 736-742.

Strasser, EF. Recovery of White Blood Cells and Platelets from Leukoreduction System Chambers of Trima Accel and COBE Spectra Plateletpheresis Devices. PDF Transfusion. Oct. 2007; 1943-1945.

Teleron AA, Carlson B, Young PP. Blood Donor White Blood Cell Reduction Filters as a Source of Human Peripheral Blood-Derived Endothelial Progenitor Cells. PDF Transfusion. Jan. 2005; 45:21-25. 

Tremblay, Mikaela M, Houtman, Jon, D. C. TCR-mediated Functions are Enhanced in Activated Peripheral Blood T Cells Isolated from Leucocyte Reduction Systems*. PDF Journal of Immunological Methods. 2015; 416: 137–145.
*Note: The UICCOM research utilized the LRS cones from UIHC’s DeGowin Blood Center in this research project.

Weitkamp JH, Crowe JE Jr. Blood Donor Leukocyte Reduction Filters as a Source of Human B Lymphocytes. PDF BioTechniques. Sept. 2001; 31:464,466.