Justin L. Grobe, PhD

Portrait
Associate Professor of Pharmacology

Contact Information

Primary Office: 2-307 BSB
51 Newton Rd.
Iowa City, IA 52242
319-353-5789

Lab: 2-300 BSB
51 Newton Rd.
Iowa City, IA 52242
319-353-5803

Education

BA, Chemistry, Hope College, Holland, MI
BS, Biology, Hope College, Holland, MI
PhD, Pharmacodynamics, University of Florida, College of Pharmacy, Gainesville, FL

Post Doctoral Fellow, Physiology and Functional Genomics, University of Florida, College of Medicine, Gainesville, FL
Post Doctoral Fellow, University of Iowa College of Medicine, Internal Medicine, Iowa City, IA

Licensure and Certifications

Fellow of the American Heart Association (FAHA)

Education/Training Program Affiliations

Department of Pharmacology Graduate Program, Interdisciplinary Graduate Program in Molecular and Cellular Biology, Interdisciplinary Graduate Program in Neuroscience, Medical Scientist Training Program

Center, Program and Institute Affiliations

Cardiovascular Research Center, Fraternal Order of Eagles Diabetes Research Center, UI Obesity Research and Education Initiative, UIHC Center for Hypertension Research

Research Summary

My laboratory is generally focused on understanding neural control of cardiovascular and metabolic function. We employ a wide range of pharmacological and physiological methods, along with new genetically-modified animal models of health and disease. Please see our laboratory website to learn about ongoing projects.

Publications

Sapouckey, S. A., Deng, G., Sigmund, C. D. & Grobe, J. L. (2017). Potential mechanisms of hypothalamic renin-angiotensin system activation by leptin and DOCA-salt for the control of resting metabolism. Physiological genomics, 49(12), 722-732. PMID: 28986397.

Riedl, R. A., Atkinson, S. N., Burnett CML,, Grobe, J. L. & Kirby, J. R. (2017). The Gut Microbiome, Energy Homeostasis, and Implications for Hypertension. Current hypertension reports, 19(4), 27. PMID: 28316052.

Agassandian, K., Grobe, J. L., Liu, X., Agassandrian, M., Thompson, A. P., Sigmund, C. D. & Cassell, M. D. (2017). Evidence for intraventricular secretion of angiotensinogen and angiotensin by the subfornical organ using transgenic mice. Am J Physiol Regul Integr Comp Physiol, 312(6), R973-R981. PMID: 28490451.

Srisai, D., Yin, T. C., Lee, A. A., Rouault AAJ,, Pearson, N. A., Grobe, J. L. & Sebag, J. A. (2017). MRAP2 regulates ghrelin receptor signaling and hunger sensing. Nature communications, 8(1), 713. PMID: 28959025.

Grobe, J. L. (2017). Comprehensive Assessments of Energy Balance in Mice. In In: The Renin-Angiotensin-Aldosterone System: Methods and Protocols. pp. 123-146. Methods Molecular Biology Series. Springer.. PMID: 28500600.

Shinohara, K., Nakagawa, P., Gomez, J., Morgan, D. A., Littlejohn, N. K., Folchert, M. D., Weidemann, B. J., Liu, X., Walsh, S. A., Ponto, L. L., Rahmouni, K., Grobe, J. L. & Sigmund, C. D. (2017). Selective Deletion of Renin-b in the Brain Alters Drinking and Metabolism. Hypertension (Dallas, Tex. : 1979), 70(5), 990-997. PMID: 28874461.

Claflin, K. E., Sandgren, J. A., Lambertz, A. M., Weidemann, B. J., Littlejohn, N. K., Burnett, C. M., Pearson, N. A., Morgan, D. A., Gibson-Corley, K. N., Rahmouni, K. & Grobe, J. L. (2017). Angiotensin AT1A receptors on leptin receptor-expressing cells control resting metabolism. The Journal of clinical investigation, 127(4), 1414-1424. PMID: 28263184.

Sandgren, J. A., Santillan, M. K. & Grobe, J. L. (2016). Breaking a Mother's Heart: Circulating Antiangiogenic Factors and Hypertension During Pregnancy Correlate With Specific Cardiac Dysfunctions. Hypertension (Dallas, Tex. : 1979), 67(6), 1119-20. PMID: 27113050.

Muntner, P., Becker, R. C., Calhoun, D., Chen, D., Cowley, Jr, A. W., Flynn, J. T., Grobe, J. L., Kidambi, S., Kotchen, T. A., Lackland, D. T., Leslie, K. K., Li, Y., Liang, M., Lloyd, A., Mattson, D. L., Mendizabal, B., Mitsnefes, M., Nair, A., Pierce, G. L., Pollock, J. S., Safford, M. M., Santillan, M. K., Sigmund, C. D., Thomas, S. J. & Urbina, E. M. (2016). Introduction to the American Heart Association's Hypertension Strategically Focused Research Network. Hypertension (Dallas, Tex. : 1979), 67(4), 674-80. PMID: 26902490.

Guo, D. F., Cui, H., Zhang, Q., Morgan, D. A., Thedens, D. R., Nishimura, D., Grobe, J. L., Sheffield, V. C. & Rahmouni, K. (2016). The BBSome Controls Energy Homeostasis by Mediating the Transport of the Leptin Receptor to the Plasma Membrane. PLoS genetics, 12(2), e1005890. PMID: 26926121.

Ketsawatsomkron, P., Keen, H. L., Davis, D. R., Lu, K. T., Stump, M., De Silva, T. M., Hilzendeger, A. M., Grobe, J. L., Faraci, F. M. & Sigmund, C. D. (2016). Protective Role for Tissue Inhibitor of Metalloproteinase-4, a Novel Peroxisome Proliferator-Activated Receptor-? Target Gene, in Smooth Muscle in Deoxycorticosterone Acetate-Salt Hypertension. Hypertension (Dallas, Tex. : 1979), 67(1), 214-22. PMID: 26597823.

Boi, S. K., Buchta, C. M., Pearson, N. A., Francis, M. B., Meyerholz, D. K., Grobe, J. L. & Norian, L. A. (2016). Obesity alters immune and metabolic profiles: New insight from obese-resistant mice on high-fat diet. Obesity (Silver Spring, Md.), 24(10), 2140-9. PMID: 27515998.

Muta, K., Morgan, D. A., Grobe, J. L., Sigmund, C. D. & Rahmouni, K. (2016). mTORC1 Signaling Contributes to Drinking But Not Blood Pressure Responses to Brain Angiotensin II. Endocrinology, 157(8), 3140-8. PMID: 27254006.

Littlejohn, N. K., Keen, H. L., Weidemann, B. J., Claflin, K. E., Tobin, K. V., Markan, K. R., Park, S., Naber, M. C., Gourronc, F. A., Pearson, N. A., Liu, X., Morgan, D. A., Klingelhutz, A. J., Potthoff, M. J., Rahmouni, K., Sigmund, C. D. & Grobe, J. L. (2016). Suppression of Resting Metabolism by the Angiotensin AT2 Receptor. Cell reports, 16(6), 1548-60. PMID: 27477281.

Shinohara, K., Liu, X., Morgan, D. A., Davis, D. R., Sequeira-Lopez, M. L., Cassell, M. D., Grobe, J. L., Rahmouni, K. & Sigmund, C. D. (2016). Selective Deletion of the Brain-Specific Isoform of Renin Causes Neurogenic Hypertension. Hypertension (Dallas, Tex. : 1979), 68(6), 1385-1392. PMID: 27754863.

Sandgren, J. A., Scroggins, S. M., Santillan, D. A., Devor, E. J., Gibson-Corley, K. N., Pierce, G. L., Sigmund, C. D., Santillan, M. K. & Grobe, J. L. (2015). Vasopressin: The Missing Link for Preeclampsia?. American journal of physiology. Regulatory, integrative and comparative physiology, ajpregu.00073.2015. PMID: 25810383.

Santillan, M. K., Pelham, C. J., Ketsawatsomkron, P., Santillan, D. A., Davis, D. R., Devor, E. J., Gibson-Corley, K. N., Scroggins, S. M., Grobe, J. L., Yang, B., Hunter, S. K. & Sigmund, C. D. (2015). Pregnant mice lacking indoleamine 2,3-dioxygenase exhibit preeclampsia phenotypes. Physiological reports, 3(1), e12257. PMID: 25602015.

Coble, J. L., Grobe, J. L., Johnson, A. K. & Sigmund, C. D. (2015). Mechanisms of brain renin angiotensin system-induced drinking and blood pressure: importance of the subfornical organ. Am J Physiol Regul Integr Comp Physiol, 308(4), R238-R249. PMID: 25519738.

Claflin, K. E., Grobe, J. L. (2015). Control of energy balance by the brain renin-angiotensin system. Current hypertension reports, 17(5), 38. PMID: 25833461.

Littlejohn, N. K., Grobe, J. L. (2015). Opposing tissue-specific roles of angiotensin in the pathogenesis of obesity, and implications for obesity-related hypertension. American journal of physiology. Regulatory, integrative and comparative physiology, 309(12), R1463-73. PMID: 26491099.

Jo, F., Jo, H., Hilzendeger, A. M., Thompson, A. P., Cassell, M. D., Rutkowski, D. T., Davisson, R. L., Grobe, J. L. & Sigmund, C. D. (2015). Brain endoplasmic reticulum stress mechanistically distinguishes the saline-intake and hypertensive response to deoxycorticosterone acetate-salt. Hypertension (Dallas, Tex. : 1979), 65(6), 1341-8. PMID: 25895586.

Barney, C. C., Schanhals, E. M., Grobe, J. L., Andresen, B. T. & Traver, M. (2015). Heat acclimation and thirst in rats. Physiological reports, 3(12), e12642. PMID: 26702076.

Bahra, S. M., Weidemann, B. J., Castro, A. N., Walsh, J. W., deLeon, O., Burnett, C. M., Pearson, N. A., Murry, D. J., Grobe, J. L. & Kirby, J. R. (2015). Risperidone-induced weight gain is mediated through shifts in the gut microbiome and suppression of energy expenditure. EBioMedicine, 2(11), 1725-34. PMID: 26870798.

Weidemann, B. J., Voong, S., Morales-Santiago, F. I., Kahn, M. Z., Ni, J., Littlejohn, N. K., Claflin, K. E., Burnett, C. M., Pearson, N. A., Lutter, M. L. & Grobe, J. L. (2015). Dietary Sodium Suppresses Digestive Efficiency via the Renin-Angiotensin System. Scientific Reports, 5, 11123. PMID: 26068176.

Coble, J. P., Johnson, R. F., Cassell, M. D., Johnson, A. K., Grobe, J. L. & Sigmund, C. D. (2014). Activity of protein kinase C-a within the subfornical organ is necessary for fluid intake in response to brain angiotensin. Hypertension, 64(1), 141-148. PMID: 24777977.

Santillan, M. K., Santillan, D. A., Scroggins, S. M., Min, J. Y., Sandgren, J. A., Pearson, N. A., Leslie, K. K., Hunter, S. K., Zamba, G. K., Gibson-Corley, K. N. & Grobe, J. L. (2014). Vasopressin in Preeclampsia: A Novel Very-Early Human Pregnancy Biomarker and Clinically-Relevant Mouse Model. Hypertension, 64(4), 852-859. PMID: 25001273.

Grobe, J. L., Sigmund, C. D. (2014). Another reason to eat your greens: cardiopulmonary protection by dietary delivery of angiotensin-converting enzyme-2 and angiotensin-(1-7) made in plants. Hypertension, 64(6), 1182-1183. PMID: 25225201.

Ye, Y., Sun, Z., Guo, A., Song, L. S., Grobe, J. L. & Chen, S. (2014). Ablation of the GNB3 gene in mice does not affect body weight, metabolism or blood pressure, but causes bradycardia. Cell Signal, 26(11), 2514-2520. PMID: 25093805.

Coble, J. P., Cassell, M. D., Davis, D. R., Grobe, J. L. & Sigmund, C. D. (2014). Activation of the renin-angiotensin system, specifically in the subfornical organ is sufficient to induce fluid intake. Am J Physiol Regul Integr Comp Physiol, 307(4), R376-R386. PMID: 24965793.

Fink, B. D., Herlein, J. A., Guo, D. F., Kulkarni, C., Weidemann, B. J., Yu, L., Grobe, J. L., Rahmouni, K., Kerns, R. J. & Sigmund, C. D. (2014). A mitochondrial-targeted coenzyme q analog prevents weight gain and ameliorates hepatic dysfunction in high-fat-fed mice. J Pharmacol Exp Ther, 351(3), 699-708. PMID: 25301169.

Shi, P., Grobe, J. L., Desland, F. A., Zhou, G., Shen, X. Z., Shan, Z., Liu, M., Raizada, M. K. & Sumners, C. (2014). Direct pro-inflammatory effects of prorenin on microglia. PLoS One, 9(10), e92937. PMID: 25302502.

Carrillo-Supulveda, M. A., Keen, H. L., Davis, D. R., Grobe, J. L. & Sigmund, C. D. (2014). Role of vascular smooth muscle PPAR? in regulating AT1 receptor signaling and angiotensin II-dependent hypertension. PLoS One, 9(8), e103786. PMID: 25122005.

Li, W., Peng, H., Mehaffey, E. p., Kimball, C. D., Grobe, J. L., van Gool, J. M., Sullivan, M. N., Earley, S., Danser, A. H., Ichihara, A. & Feng, Y. (2013). Neuron-Specific (Pro)renin Receptor Knockout Prevents the Development of Salt-Sensitive Hypertension. Hypertension, 63(2), 316-323. PMID: 24246383.

Grobe, J. L., Rahmouni, K., Liu, X. & Sigmund, C. D. (2013). Metabolic rate regulation by the renin-angiotensin system: brain vs. body. Pflugers Arch - European J of Physiology, 465(1), 167-175. PMID: 22491893.

Hilzendeger, A. M., Cassell, M. D., Davis, D. R., Stauss, H. M., Mark, A. L., Grobe, J. L. & Sigmund, C. D. (2013). Angiotensin Type 1a Receptors in the Subfornical Organ Are Required for Deoxycorticosterone Acetate-Salt Hypertension. Hypertension, 61(3), 716-722. PMID: 23266541.

Grobe, J. L., Rahmouni, K. (2012). Editoral Focus: A fat contribution to RAS activation and blood pressure control: evidence from angiotensinogen conditional null mice. Focus on: "Adipocyte-specific deficiency of angiotensinogen decreases plasma angiotensinogen concentration and systolic blood pressure in mice. Am J Physiol: Regul Integr Comp Physiol, 302(2), R242-R243. PMID: 22071163.

Grobe, J. L., Rahmouni, K. (2012). The adipose/circulating renin-angiotensin system cross-talk enters a new dimension. Hypertension, 60(6), 1389-1390. PMID: 23108659.

Pelham, C. J., Ketsawatsomkron, P., Groh, S., Grobe, J. L., de Lange, W. J., Ibeawuchi, S. C., Keen, H. L., Weatherford, E. T., Faraci, F. F. & Sigmund, C. D. (2012). Cullin-3 Regulates Vascular Smooth Muscle Function and Arterial Blood Pressure via PPAR? and RhoA/Rho-Kinase. Cell Metab, 16(4), 462-472. PMID: 23040068.

Hilzendeger, A. M., Morgan, D. A., Brooks, L., Dellsperger, D., Liu, X., Grobe, J. L., Rahmouni, K., Sigmund, C. D. & Mark, A. L. (2012). A brain leptin-renin angiotensin system interaction in the regulation of sympathetic nerve activity. Am J Physiol Heart Circ Physiol, 303(2), H197-H206. PMID: 22610169.

Ketsawatsomkron, P., Lorca, R. A., Keen, H. L., Weatherford, E. T., Liu, X., Pelham, C. j., Grobe, J. L., Faraci, F. M., England, S. K. & Sigmund, C. D. (2012). PPAR? regulates resistance vessel tone through a mechanism involving RGS5-mediated control of protein kinase C and BKCa channel activity. Circ Res, 111(11), 1446-1458. PMID: 22962432.

Grobe, J. L., Buehrer, B. A., Hilzendeger, A. M., Liu, X., Davis, D. R., Xu, D. & Sigmund, C. D. (2011). Angiotensinergic signaling in the brain mediates metabolic effects of deoxycorticosterone (DOCA)-salt in C57 mice. Hypertension, 57[Part 2}(3), 600-607. PMID: 21263123.

Li, H., Weatherford, E. T., Davis, D. R., Keen, H. L., Grobe, J. L., Daugherty, A., Cassis, L. A., Allen, A. M. & Sigmund, C. D. (2011). Renal proximal tubule angiotensin AT1A receptors regulate blood pressure. Am J Physiol Regul Integr Comp Physiol, 301(4), R1067-R1077. PMID: 21753145.

Xu, D., Borges, G. R., Davis, D. R., Agassandian, K., Sequeira Lopez, M. L., Gomez, R. A., Cassell, M. D., Grobe, J. L. & Sigmund, C. D. (2011). Neuron- or glial-specific ablation of secreted renin does not affect renal renin, baseline arterial pressure, or metabolism. Physiol Genomics, 43(6), 286-294. PMID: 21189370.

Grobe, J. L., Grobe, C. L., Beltz, T. G., Westphal, S. G., Morgan, D. A., Xu, D., de Lange, W. J., Li, H., Sakai, K., Thedens, D. R., Cassis, L. A., Rahmouni, K., Mark, A. L., Johnson, A. K. & Sigmund, C. D. (2010). The brain Renin-angiotensin system controls divergent efferent mechanisms to regulate fluid and energy balance. Cell Metabolism, 12(5), 431-442. PMID: 21035755.

Grobe, J. L., Dickson, M. E., Park, S., Davis, D. R., Born, E. J. & Sigmund, C. D. (2010). Cardiovascular consequences of genetic variation at -6/235 in human angiotensinogen using "humanized" gene-targeted mice. Hypertension, 56(5), 981-987. PMID: 20823378.

Shenov, V., Grobe, J. L., Qi, Y., Ferreira, A. J., Fraga-Silva, R. A., Collamat, G., Bruce, E. & Katovich, M. J. (2009). 17beta-Estradiol modulates local cardiac renin-angiotensin system to prevent cardiac remodeling in the DOCA-salt model of hypertension in rats. Peptides, 30(12), 2309-2315. PMID: 19747516.

Xu, D., Borges, G. R., Grobe, J. L., Pelham, C. J., Yang, B. & Sigmund, C. D. (2009). Preservation of intracellular renin expression is insufficient to compensate for genetic loss of secreted renin. Hypertension, 54(6), 1240-1247. PMID: 19822797.

Grobe, J. L., Venegas-Pont, M., Sigmund, C. D. & Ryan, M. J. (2009). PPARgamma differentially regulates energy substrate handling in brown vs. white adipose: focus on "The PPARgamma agonist rosiglitazone enhances rat brown adipose tissue lipogenesis from glucose without altering glucose uptake". Am J Physiol Regul Integr Comp Physiol, 296(5), R1325-R1326.

Grobe, J. L., Xu, D. & Sigmund, C. D. (2008). An intracellular renin-angiotensin system in neurons: fact, hypothesis, or fantasy. Physiology (Bethesda), 23, 187-193. PMID: 18697992.

Der Sarkissian, S., Grobe, J. L., Yuan, L., Narielwala, D. R., Walter, G. A., Katovich, M. J. & Raizada, M. K. (2008). Cardiac overexpression of angiotensin converting enzyme 2 protects the heart from ischemia-induced pathophysiology. Hypertension, 51(3), 712-718. PMID: 18250366.

Katovich, M. J., Grobe, J. L. & Raizada, M. K. (2008). Angiotensin-(1-7) as an antihypertensive, antifibrotic target. Curr Hypertens Rep, 10(3), 227-232. PMID: 18765095.

Li, H., Gao, Y., Grobe, J. L., Raizada, M. K., Katovich, M. J. & Sumners, C. (2007). Potentiation of the antihypertensive action of losartan by peripheral overexpression of the ANG II type 2 receptor. Am J Physiol Heart Circ Physiol, 2992(2), H727-H735. PMID: 17085538.

Grobe, J. L., Mecca, A. P., Lingis, M., Shenoy, V., Bolton, T. A., Machado, J. M., Speth, R. C., Raizada, M. K. & Katovich, M. J. (2007). Prevention of angiotensin II-induced cardiac remodeling by angiotensin-(1-7). Am J Physiol Heart Circ Physiol, 292(2), H736-H742. PMID: 17098828.

Grobe, J. L., Der Sarkissian, S., Stewart, J. M., Meszaros, J. G., Raizada, M. K. & Katovich, M. J. (2007). ACE2 overexpression inhibits hypoxia-induced collagen production by cardiac fibroblasts. Clin Sci (Lond), 113(8), 357-364. PMID: 17600530.

Grobe, J. L., Katovich, M. J. (2006). Alterations in aortic vascular reactivity to angiotensin 1-7 in 17-beta-estradiol-treated female SD rats. Regul Pept, 133(1-3), 62-67. PMID: 16219374.

Grobe, J. L., Mecca, A. P., Mao, H. & Katovich, M. J. (2006). Chronic angiotensin-(1-7) prevents cardiac fibrosis in DOCA-salt model of hypertension. Am J Physiol Heart Circ Physiol., 290(6), H2417-H2423. PMID: 16415071.

Katovich, M. J., Grobe, J. L., Huentelman, M. & Raizada, M. K. (2005). Angiotensin-converting enzyme 2 as a novel target for gene therapy for hypertension. Exp Physiol, 90(3), 299-305. PMID: 15640278.

Katovich, M. J., Grobe, J. L. & Raizada, M. K. (2005). Gene therapy for hypertension: current targets and consideration of novel targets. In In: Cardiovascular Genomics. pp. 213-246. Humana Press.

Huentelman, M. J., Grobe, J. L., Vazquez, J., Stewart, J. M., Mecca, A. P., Katovich, M. J., Ferrario, C. M. & Raizada, M. K. (2005). Protection from angiotensin II-induced cardiac hypertrophy and fibrosis by systemic lentiviral delivery of ACE2 in rats. Exp Physiol, 90(5), 783-790. PMID: 16049057.

Grobe, J. L., Katovich, M. & Rowland, N. (2004). Role of angiotensin II in the subfornical organ in the pharmacological actions of ethanol. Alcohol and Alcoholism, 39(5), 410-417. PMID: 15289207.

Barney, C. C., Kurylo, D. M. & Grobe, J. L. (2003). Thermal dehydration-induced thirst in lithium-treated rats. Pharmacol Biochem Behav, 75(2), 341-347. PMID: 12873625.

Burnatowska-Hledin, M., Zeneberg, A., roulo, A., Grobe, J. L., Zhao, P., Lelkes, P. I., Clare, P. & Barney, C. (2001). Expression of VACM-1 protein in cultured rat adrenal endothelial cells is linked to the cell cycle. Endothelium, 8(1), 49-63. PMID: 11409851.