Yuriy M. Usachev, PhD

Portrait
Professor of Pharmacology
Professor of Anesthesia
John P. Long Professor

Contact Information

Primary Office
2-450 BSB
51 Newton Rd.
Iowa City, IA 52242
319-335-9388

Lab
2-440 BSB
Iowa City, IA 52242
319-353-3887

Education

BS, Physics - Optics and Spectroscopy, Kiev State University, Kiev, Ukraine
MSc, Physics—Optics and Spectroscopy, Kiev State University, Kiev, Ukraine
PhD, Biology, International Center of Molecular Physiology, Bogomoletz Institute of Physiology, Kiev, Ukraine
Fellow, The Physiological Laboratory, University of Cambridge, Cambridge, United Kingdom
Fellow, Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota

Education/Training Program Affiliations

Interdisciplinary Graduate Program in Neuroscience, Medical Scientist Training Program

Research Summary

Chronic pain management remains one of the most serious public health problems. We use an array of molecular biological and genetic techniques, combined with patch-clamp recordings and fluorescent imaging of intracellular Ca2+, Na+ and pH changes in pain-conducting neurons (called nociceptors), as well as behavioral studies to address two broad sets of questions related to chronic pain pathogenesis. The first set of questions focuses on relatively rapid changes to nociceptor excitability and synaptic transmission that are induced by proinflammatory mediators generated by immune and glial cells at the site of injury or inflammation, and mediated via phosphorylation of so-called pain channels, including TRPV1, TRPA1 and voltage-gated Na+ channels Nav1.7, 1.8 and 1.9. We are particularly interested in the role of the complement system factors C3a and C5a in regulating nociceptor excitability and function. The second set of questions examines the long-term changes to the nociceptor molecular composition and function in response to injury or inflammation, and involves alterations in gene expression. We particularly focus on the Ca2+-dependent transcription factor NFAT that regulates expression of a number of proteins implicated in pain sensitization, such as COX-2, BDNF, chemokine receptor CCR2 and several voltage-gated K+ channels. Another line of investigation focuses on the function of mitochondria in regulating neuronal plasticity and survival under conditions of stress or illness, such as hyperglycemia in diabetes or exposure to toxic concentrations of glutamate following ischemic stroke. In addition to generating energy, mitochondria play critical role in neuronal signaling, and particularly, in the regulation of Ca2+ homeostasis and Ca2+-dependent processes. Impaired mitochondrial Ca2+ regulation contributes to neuronal damage in stroke and neurodegenerative disorders. Mitochondria are highly dynamic organelles that can rapidly undergo fission and fusion, and the mitochondrial fission and fusion (MFF) balance can significantly impact dendritic and axonal morphogenesis, synaptic plasticity and neuronal survival. We use a multidisciplinary approach (electrophysiology, confocal microscopy, brain slice recordings, animal models of diabetes and stroke) to better understand regulatory mechanisms and function of mitochondrial Ca2+ transport in neurons, its dependence on the MFF status, and the role of mitochondrial Ca2+ and MFF in protecting neurons from glutamate toxicity as well as hyperglycemia-induced axonal degeneration.

Publications

Warwick, C. A., Usachev, Y. M. (2017). Culture, Transfection, and Immunocytochemical Analysis of Primary Macrophages. Methods Mol Biol, 1554, 161-173. PMID: 28185189.

Shutov, L. P., Warwick, C. A., Shi, X., Gnanasekaran, A., Shepherd, A. J., Mohapatra, D. P., Woodruff, T. M., Clark, J. D. & Usachev, Y. M. (2016). The complement system component C5a produces thermal hyperalgesia via macrophage-to-nociceptor signaling that requires NGF and TRPV1. Journal of Neuroscience, 36(18), 5055-5070. PMID: 27147658.

Inagaki, A., Frank, A. C., Usachev, Y. M., Benveniste, M. & Lee, A. (2014). Pharmacological correction of gating defects in the voltage- gated Cav2.1 Ca2+ channel due to a familial hemiplegic migraine mutation. Neuron, 81, 91-102. PMID: PMID: 24411734 [PubMed - in process].

Wu, J., Prole, D. L., Shen, Y., Lin, Z., Gnanasekaran, A., Liu, Y., Chen, L., Zhou, H., Chen, S. W., Usachev, Y. M., Taylor, C. W. & Campbell, R. E. (2014). Red fluorescent genetically encoded Ca2+ indicators for mitochondria and endoplasmic reticulum. The Biochemical Journal, 464(1), 13-22. PMID: 25164254.

Kim, M. S., Shutov, L. P., Gnanasekaran, A., Lin, Z., Rysted, J. E., Ulrich, J. D. & Usachev, Y. M. (2014). Nerve growth factor (NGF) regulates activity of the transcription factor NFAT in neurons via the phosphatidylinositol 3-kinase (PI3K)-Akt-glycogen synthase kinase 3beta (GSK3beta) pathway. Journal of Biological Chemistry, 289(45), 31349-31360. PMID: 25231981.

Shutov, L. p., Kim, M., Houlihan, P. R., Medvedeva, Y. V. & Usachev, Y. M. (2013). Mitochondria and plasma membrane Ca2+ -ATPase (PMCA) control presynaptic clearance in capsaicin-sensitive rat sensory neurons. Journal of Physiology, 591, 2443-2462.

Sowers, L. P., Loo, L., Wu, Y. M., Campbell, E., Ulrich, J. D., Wu, S., Paemka, L., Wassink, T., Meyer, K., Bing, X., El-Shanti, H., Usachev, Y. M., Ueno, N., manak, R. j., Shepherd, A. J., Ferguson, P. J., Richerson, G. B., Mohapatra, D. P., Wemmie, J. A. & Bassuk, A. G. (2013). PRICKLE2 defects link the non-canonical Wnt pathway to autism. Molecular Psychiatry, 18, 1077-1089.

Ulrich, J. D., Kim, M. S., Houlihan, P. R., Shutov, L. P., Mohapatra, D. P., Strack, S. & Usachev, Y. M. (2012). Distinct Activation Properties of the Nuclear Factor of Activated T-cells (NFAT) Isoforms NFATc3 and NFATc4 in Neurons. (Vols. 287). (45), pp. 37594-37609. J Biol Chem.

Loo, L., Shepherd, A. J., Mickle, A. D., Lorca, R. A., Shutov, L. P., Usachev, Y. M. & Mohapatra, D. P. (2012). The C-Type Natriuretic Peptide Induces Thermal Hyperalgesia through a Noncanonical Gß?-dependent Modulation of TRPV1 Channel. J Neurosci, 32(35), 11942-11955.

Merrill, R. A., Dagda, R. K., Dickey, A. S., Cribbs, J. T., Green, S. H., Usachev, Y. M. & Strack, S. (2011). Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1. PLoS Biol, 9(4), e1000612.

Jenkins, M. A., Christel, C. J., Jiao, Y., Abiria, S., Kim, K. Y., Usachev, Y. M., Obermair, G. J., Colbran, R. J. & Lee, A. (2010). Ca2+-dependent facilitation of Cav1.3 Ca2+ channels by densin and Ca2+/calmodulin-dependent protein kinase II. J Neurosci, 30(15), 5125-5135.

Jang, J. H., Clark, J. D., Li, X., Yorel, M. S., Usachev, Y. M. & Brennan, T. J. (2010). Nociceptive sensitization by complement C5a and C3a in mouse. Pain, 148(2), 343-352.

Kim, M., Usachev, Y. M. (2009). Mitochondrial Ca2+ cycling facilitates activation of the transcription factor NFAT in sensory neurons. J Neurosci, 29(39), 12101-12114.

Medvedeva, Y. V., Kim, M., Schnizler, K. & Usachev, Y. M. (2009). Functional tetrodotoxin-resistant Na(+) channels are expressed presynaptically in rat dorsal root ganglia neurons. Neuroscience, 159(2), 559-569. PMID: PMID: 19162133.

Dagda, R. K., Merrill, R. A., Cribbs, J. T., Chen, Y., Hell, J. W., Usachev, Y. M. & Strack, S. (2008). The spinocerebellar ataxia 12 gene product and protein phosphatase 2A regulatory subunit Bbeta2 antagonizes neuronal survival by promoting mitochondrial fission. J Biol Chem, 283(52), 36241-36248.

Medvedeva, Y. V., Kim, M. S. & Usachev, Y. M. (2008). Mechanisms of prolonged presynaptic Ca2+ signaling and glutamate release induced by TRPV1 activation in rat sensory neurons. J Neurosci, 28(20), 5295-5311.

Schnizler, K., Shutov, L. P., Van Kanegan, M. J., Merrill, M. A., Nichols, B., McKnight, G. S., Strack, S., Hell, J. W. & Usachev, Y. M. (2008). Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse sensory neurons. J Neurosci, 28(19), 4904-4917.

Sugiyama, D., Kang, S., Arpey, N., Arunakul, P., Usachev, Y. M. & Brennan, T. J. (2017). Hydrogen Peroxide Induces Muscle Nociception via Transient Receptor Potential Ankyrin 1 Receptors. Anesthesiology, 127(4), 695-708. PMID: 28640016.

Wu, J., Prole, D. L., Shen, Y., Lin, Z., Gnanasekaran, A., Liu, Y., Chen, L., Zhou, H., Chen, S. R., Usachev, Y. M., Taylor, C. W. & Campbell, R. E. (2014). Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum. The Biochemical journal, 464(1), 13-22. PMID: 25164254.

Kim, M. S., Shutov, L. P., Gnanasekaran, A., Lin, Z., Rysted, J. E., Ulrich, J. D. & Usachev, Y. M. (2014). Nerve growth factor (NGF) regulates activity of nuclear factor of activated T-cells (NFAT) in neurons via the phosphatidylinositol 3-kinase (PI3K)-Akt-glycogen synthase kinase 3ß (GSK3ß) pathway. The Journal of biological chemistry, 289(45), 31349-60. PMID: 25231981.

Inagaki, A., Frank, C. A., Usachev, Y. M., Benveniste, M. & Lee, A. (2014). Pharmacological correction of gating defects in the voltage-gated Ca(v)2.1 Ca²? channel due to a familial hemiplegic migraine mutation. Neuron, 81(1), 91-102. PMID: 24411734.

Shutov, L. P., Warwick, C., Gupte, R., Shepherd, A., Mohapatra, D. P. & Usachev, Y. M. (2014). The complement fragment C5a produces thermal hyperalgesia via TRPV1 and macrophage-dependent release of NGF.

Gong, W., Kolker, S. J., Usachev, Y. M., Walder, R. Y., Boyle, D., Firestein, G. S. & Sluka, K. A. (2014). Acid-Sensing Ion Channel 3 decreases phosphorylation of ERK and induces synoviocyte cell death by increasing intracellular calcium.

Kim, M., Shutov, L. P., Ulrich, J. D., Lin, Z., Rysted, J. & Usachev, Y. M. (2014). NGF facilitates depolarization-induced activation of Ca2+ /calcineurin-dependent transcription NFAT in sensory neurons via the PI3K-Akt-GSK3? signaling pathway.

Shutov, L. P., Warwick, C. A., Gupte, R. P., Shepherd, A. J., Brennan, T. J., Clark, J. D., Mohapatra, D. P. & Usachev, Y. M. (2013). Complement C5a produces nociceptive sensitization via TRPV1 in mouse. pp. Program No. 642.16. Abstract Viewer/Itinerary Planner. San Diego, CA: Society for Neuroscience (Poster).

Yorek, M. S., Houlihan, P. R., Slupe, A. & Usachev, Y. M. (2011). Mechanisms of TRPM8-mediated [Ca2+]i and electrical oscillations in sensory neurons. IBRO 8th World Congress of Neuroscience (Poster), Florence, Italy.

Shutov, L. P., Kim, M. & Usachev, Y. M. (2011). Mitochondria and plasma membrane Ca2+-ATPase (PMCA) control presynaptic [Ca2+]i clearance in capsaicin-sensitive sensory neurons. pp. Program No. 381.11. Abstract Viewer/Itinerary Planner. Washington DC: Society for Neuroscience (Poster).

Houlihan, P. R., Dickey, A., Merrill, R., Strack, S. & Usachev, Y. M. (2011). Mitochondria-targeted protein kinase A stabilizes Ca2+ homeostasis in hippocampal neurons exposed to toxic concentrations of glutamate. pp. Program No. 465.10. Abstract Viewer/Itinerary Planner. Washington DC: Society for Neuroscience (Poster).

Loo, L., Shepherd, A. J., Lorca, R. A., Mickle, A. D., Usachev, Y. M. & Mohapatra, D. P. (2011). Natriuretic peptides induce nociceptor sensitization by upregulating TRPV1 activity/expression. pp. Program No. 380.22. Abstract Viewer/Itinerary Planner. Washington DC: Society for Neuroscience (Poster).

Kolker, S. J., Walder, R. Y., Usachev, Y. M., Hillman, J., Boyle, D. L., Firestein, G. & Sluka, K. (2010). ASIC3 expressed in Type B synoviocytes and chondrocytes modulates hyaluronan expression and release. Annals of the Rheumatic Diseases, 69(5), 903-909.

Houlihan, P. R., Dickey, A., Merrill, R. A., Strack, S. & Usachev, Y. M. (2010). Phosphoregulation of mitochondrial architecture and excitotoxicity in hippocampal neurons. pp. Program No. 873.9. Abstract Viewer/Itinerary Planner. San Diego, CA: Society for Neuroscience (Poster).

Ulrich, J. D., Kim, M. & Usachev, Y. M. (2010). Distinct regulation of NFATc3 and NFAC4 in central and peripheral neurons. pp. Program No. 167.4. Abstract Viewer/Itinerary Planner. San Diego, CA: Society for Neuroscience (Poster).

Yorek, M. S., R, H. P., Slupe, A. & Usachev, Y. M. (2010). Mechanisms of TRPM8-mediated [Ca2+]i oscillations in sensory neurons. pp. Program No. 281.7. Abstract Viewer/Itinerary Planner. San Diego, CA: Society for Neuroscience (Poster).

Kim, M., Usachev, Y. M. (2009). NGF activates NFAT-dependent transcription via the PI3K-Akt-GSK3b pathway in rat sensory neurons. (Vols. 23). (1), pp. 884.1. FASEB J.

Kim, M., Usachev, Y. M. (2009). NGF facilitates depolarization-induced NFAT activation via the PI3K-Akt-GSK3b pathway in DRG sensory neurons. pp. Program No. 711.12. Abstract Viewer/Itinerary Planner. Chicago, IL: Society for Neuroscience (Poster)..

Merrill, R. A., Dagda, R. K., Cribbs, J. T., Green, S. H., Usachev, Y. M. & Strack, S. (2009). PKA/AKAP1 inhibits fission via Drp1 to promote neuronal survival. pp. Program No. 634.9. Abstract Viewer/Itinerary Planner. Chicago, IL: Society for Neuroscience (Poster)..

Houlihan, P. R., Slupe, A. M., Yorek, M. S. & Usachev, Y. M. (2009). Mechanisms of Ca2+ and electrical oscillations induced by minimal activation of TRPM8 in a subset of DRG neurons. pp. Program No. 761.12. Abstract Viewer/Itinerary Planner. Chicago, IL: Society for Neuroscience (Poster)..

Lu, Y., Zhang, M., Lim, I. A., Hall, D. D., Allen, M., Medvedeva, Y., McKnight, G. S., Usachev, Y. M. & Hell, J. W. (2008). AKAP150-anchored PKA activity is important for LTD during its induction phase. J Physiol, 586(Pt 17), 4155-4164.

Medvedeva, Y. V., Kim, M., Schnizler, K. & Usachev, Y. M. (2008). Functional tetrodotoxin-resistant Na+ channels are expressed presynaptically in rat DRG neurons. Neuroscience, 159(2), 559-569. PMID: 19162133.

Usacheva, M. N., Teichert, M. C., Usachev, Y. M., Sievert, C. E. & Biel, M. A. (2008). Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas Aeruginosa cells. Lasers in Surgery and Medicine, 40(1), 55-61. PMID: 18220267.

Lu, Y., Allen, M., Halt, A. R., Weisenhaus, M., Dallapiazza, R. F., Hall, D. D., Usachev, Y. M., McKnight, S. G. & Hell, J. W. (2007). Age-dependent requirement of AKAP150-anchored PKA and GluR2-lacking AMPA receptors in LTP. EMBO J, 26(23), 4879-4890.

Jackson, J. G., Usachev, Y. M. & Thayer, S. A. (2007). Bradykinin-induced NFAT-dependent transcription in rat dorsal root ganglion neurons. Mol. Pharmacology, 72(2), 303-310. PMID: 17485565.

Schnizler, K., Van Kanegan, M., Merrill, M., McKnight, S., Strack, S., Hell, J. & Usachev, Y. M. (2007). Anchoring of PKA via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse DRG neurons. pp. Program No. 400.21. 2007 Abstract Viewer/Itinerary Planner. San Diego, CA: Society for Neuroscience (Poster).

Strack, S., Dagda, R. K., cribbs, J. T., Chen, Y., Hell, J. W., Green, S. H. & Usachev, Y. M. (2006). Outer Mitochondrial PP2A and PKA Control Neuronal Survival Through Mitochondrial Restructuring. pp. Presentation No. 19.6. Abstract Viewer/Itinerary Planner. Atlanta, GA: Society for Neuroscience (Slide Session).

Usachev, Y. M., Marsh, A. j., Johanns, T. M., Lemke, M. M. & Thayer, S. A. (2006). Activation of protein kinase C in sensory neurons accelerates Ca2+ uptake into the endoplasmic reticulum. J of Neurosci, 26(1), 311-318.

Medvedeva, Y. V., Usachev, Y. M. (2006). Functional Expression of Presynaptic Tetrodotoxin-Resistant Sodium Channels in Rat Sensory Neurons. pp. Program No. 245.9. Abstract Viewer/Itinerary Planner. Atlanta, GA: Society for Neuroscience (Poster).

Kim, M., Thayer, S. A. & Usachev, Y. M. (2006). Mitochondrial Regulate Activation of the Transcription Factor NFAT in DRG Neurons. pp. Program No. 321.9. Abstract Viewer/Itinerary Planner. Atlanta, GA: Society for Neuroscience (Poster).

Dagda, R. K., Usachev, Y. M. & Strack, S. (2005). Dynamic regulation of mitochondrial morphology, calcium buffering, and neuronal survival by a neuron-specific protein phosphatase 2A holoenzyme. pp. Program No. 785.14. Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience (Poster).

Kim, M., Thayer, S. A., Smirnov, D. & Usachev, Y. M. (2005). Mitochondrial Ca2+ cycling facilitates activation of the transcription factor NFAT in sensory neurons. pp. Program No. 484.3. Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience.

Medvedeva, Y. V., Usachev, Y. M. (2005). Vanilloid-induced presynaptic Ca2+ signaling and glutamate release in rat sensory neurons: The role of mitochondria. pp. Program No. 961.5. Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience.

Usachev, Y. M., Johanns, T. M., Lemke, M. M. & Thayer, S. A. (2004). Activation of protein kinase C in sensory neurons accelerates calcium uptake into the endoplasmic reticulum. pp. Program No. 165.17. Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience.

Medvedeva, Y. V., Usachev, Y. M. (2004). Mitochondrial calcium cycling in sensory axons controls long-lasting synaptic activity induced by vanilloids. pp. Program No. 860.7. Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience.

Usachev, Y. M., DeMarco, S. J., Campbell, C., Strehler, E. E. & Thayer, S. A. (2002). Bradykinin and ATP accelerate Ca2+ efflux from rat sensory neurons via protein kinase C and the plasma membrane Ca2+ pump isoform 4. Neuron, 33(1), 113-122. PMID: 11779484.

Thayer, S. A., Usachev, Y. M. & Pottorf, W. J. (2002). Modulating Ca2+ clearance from neurons (invited review). Frontiers in Bioscience, 7, 1255-1279.

Garcia, M. L., Usachev, Y. M., Thayer, S. A., Strehler, E. E. & Windenbank, A. J. (2001). The plasma membrane ATPase plays a role in reducing Ca2+ mediated cytotoxicity in PC12 cells. J Neuroscience Research, 64(6), 661-669. PMID: 11398191.

Usachev, Y. M., Toutenhoofd, S. L., Goellner, G. M., Strehler, E. E. & Thayer, S. A. (2001). Differentiation induces upregulation of plasma membrane Ca2+ -ATPase and concomitant increase in Ca2+ efflux in human neuroblastoma cell line IMR-32. J Neurochemistry, 76(6), 1756-1765. PMID: 11259493.

Usachev, Y. M., DeMarco, S. J., Campbell, C., Strehler, E. E. & Thayer, S. A. (2001). Bradykinin and ATP accelerate Ca2+ efflux from sensory neurons: the role of protein kinase C and the plasma membrane Ca2+ pump isoform 4. (Vols. 27). pp. Program No. 44.5. Society for Neuroscience Abstracts.

Usachev, Y. M., Khammanivong, A., Campbell, C. & Thayer, S. A. (2000). Particle-mediated gene transfer to rat neurons in primary culture. Pflugers Archiv, 439(6), 730-738. PMID: 10784347.

Usachev, Y. M., Thayer, S. A. (1999). Ca2+ influx in resting rat sensory neurons that regulates and is regulated by ryanodine-sensitive Ca2+ stores. J of Physiology, 519(pt. 1), 115-130.

Usachev, Y. M., Thayer, S. A. (1999). Controlling the urge for a Ca2+ surge: all-or-none calcium release in neurons (invited review). BioEssays, 21, 743-750.

Usachev, Y., Khammanivong, A., Strehler, E. & Thayer, S. (1999). Modulation of Ca2+ efflux from cultured rat dorsal root ganglion (DRG) neurons by protein kinase C. (Vols. 25). pp. 1191. Society for Neuroscience Abstracts.

Usacheva, M., Usachev, Y., Teichert, M. & Biel, M. (1999). The role of methylene blue and toluidine blue monomers and dimers in inactivation bacteria. (Vols. 69). pp. 565. Photochemistry and Photobiology.

Usachev, Y., Thayer, S. (1998). Co-transfection of primary neurons using particle-mediated gene delivery. (Vols. 24). pp. 72. Society for Neuroscience Abstracts.

Thayer, S., Usachev, Y., Werth, J. & Baron, K. (1998). Modulation of Ca2+ signaling in cultured rat dorsal root ganglion neurones. pp. 506P, 10S. J. of Physiology.

Usachev, Y. M., Thayer, S. A. (1997). All-or-none Ca2+ release from intracellular stores triggered by Ca2+ influx through voltage-gated Ca2+ channels in rat sensory neurons. J Neuroscience, 17(19), 7404-7414. PMID: 9295386.

Usachev, Y., Thayer, S. (1997). The mechanism of refilling ryanodine-sensitive Ca2+ stores in rat sensory neurons. (Vols. 23). pp. 1192. Society for Neuroscience Abstracts.

Usachev, Y., Thayer, S. (1996). All-or-none Ca2+ release from intracellular Ca2+ stores in rat DRG neurons. (Vols. 22). pp. 340. Society for Neuroscience Abstracts.

Werth, J. l., Usachev, Y. M. & Thayer, S. A. (1996). Modulation of Ca2+ efflux from cultured rat DRG neurons. J Neuroscience, 16(3), 1008-1015. PMID: 8558228.

Usachev, Y. M., Marchenko, S. M. & Sage, S. O. (1995). Cytosolic calcium concentration in resting and stimulated endothelium of excised intact rat aorta. J Physiology, 489(pt. 2), 309-317.

Usachev, Y. M., Kostyuk, P. & Verkhratsky, A. (1995). 3-Isobutyl-1-methylxanthine (IBMX) affects potassium permeability in rat sensory neurones via pathways that are sensitive and insensitive to [Ca2+]in. Pflugers Archiv, 430(3), 420-428. PMID: 7491267.

Usachev, Y. M., Verkhratsky, A. (1995). IBMX induces calcium release from intracellular stores in rat sensory neurons. Cell Calcium, 17(3), 197-206. PMID: 7542569.

Usachev, Y., Marchenko, S. & Sage, S. (1995). Agonist-evoked changes in cytosolic calcium concentration in the endothelium of excised intact rat aorta. pp. 483P, 137P. J. of Physiology.

Usachev, Y. M., Verkhratsky, A. (1995). IBMX induces Ca2+ release from internal stores and affects K+ channels in isolated rat sensory neurones. pp. 483P, 67P. J. of Physiology.

Shmigol, A., Usachev, Y. M., Kirischuk, S., Kostyuk, P. & Verkhratsky, A. (1994). Properties of the caffeine-sensitive Ca2+ stores in mammalian neurons. Neurophysiology, 26, 16-24.

Usachev, Y. M., Shmigol, A., Pronchuk, N. & Verkhratsky, A. (1994). The role of caffeine-sensitive calcium stores in modulation of calcium signals in rat sensory neurons. Proceedings of Russian Academy of Sciences, 334, 381-384.

Usachev, Y. M., Shmigol, A., Pronchuk, N., Kostyuk, P. & Verkhratsky, A. (1993). Caffeine-induced calcium release from internal stores in cultured rat sensory neurons. Neuroscience, 57(3), 845-859. PMID: 8309540.

Mironov, S., Usachev, Y. M. & Lux, H. (1993). Spatial and temporal control of intracellular Ca dynamics in chick DRG neurons. Pflugers Archiv, 424(2), 183-191. PMID: 8414905.

Mironov, S., Usachev, Y. M. (1991). Caffeine affects Ca uptake and Ca release from intracellular stores: fura-2 measurements in isolated snail neurons. Neuroscience Letters, 123, 200-202.

Usachev, Y. M., Mironov, S. (1991). Effect of caffeine on intracellular Ca regulation processes in isolated snail neurons. Neurophysiology, 23, 66-73.

Mironov, S., Usachev, Y. M. (1990). Sr and Ba transients in isolated snail neurons studied with fura-2. The recovery from depolarization induced load and modulation of Ca release from intracellular stores. Neuroscience Letters, 112, 184-189.

Usachev, Y. M., Mironov, S. (1989). Effect of Sr and Ba ions on Ca binding and transport in nerve cells. Neurophysiology, 21, 820-825.

Shutov, L. P., Usachev, Y. M. Mitochondria and PMCA regulate presynaptic [Ca2+]i in sensory neurons. IASP 14th World Congress on Pain (Poster), Milan, Italy.