Nikolai Artemyev, PhD

Portrait
Professor of Molecular Physiology and Biophysics
Professor of Ophthalmology and Visual Sciences

Contact Information

Office: 5-532 Bowen Science Building
Iowa City, IA 52242
319-335-7864

Lab: 5-533 Bowen Science Building
Iowa City, IA 52242
319-335-7865

Fax:
319-335-7330

Education

BS, Biotechnology, St. Petersburg Institute of Technology
PhD, St. Petersburg Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

Education/Training Program Affiliations

Department of Molecular Physiology and Biophysics PhD, Interdisciplinary Graduate Program in Molecular and Cellular Biology, Interdisciplinary Graduate Program in Translational Biomedicine, Medical Scientist Training Program

Research Summary

"Heterotrimeric GTP-binding proteins (G-proteins) are used by cells to transduce signals from the extracellular environment through surface receptors to intracellular effector proteins. One of the best model systems for studying mechanisms of G protein signaling is the rod photoreceptor visual transduction cascade. Our laboratory focuses on investigation of specific protein-protein interactions between the photoreceptor – rhodopsin, the rod G protein - transducin, and the effector enzyme - cGMP-phosphodiesterase. We study the modulation of transducin by retinal regulators of G protein signaling (RGS) and other accessory proteins. To understand the activation and turn-off mechanisms of transducin and cGMP-phosphodiesterase upon transduction of a visual signal we employ tools and techniques such as transgenic animal models, site-directed mutagenesis, synthetic peptides, site specific antibodies, cross-linking, and fluorescence spectroscopy. The high levels of sequence homology between different types of G proteins, as well as the structural similarities observed in their crystal structures suggest that numerous G protein signaling systems have similar mechanisms of activation. Our ultimate goal is to understand the mechanisms of regulation of G protein signaling at the molecular level." PubMed link to publications: http://www.ncbi.nlm.nih.gov/pubmed?term=artemyev%20n

Publications

Gopalakrishna, K. N., Boyd, K. & Artemyev, N. O. (2017). Mechanisms of mutant PDE6 proteins underlying retinal diseases. Cellular signalling, 37, 74-80. PMID: 28583373.

Yadav, R. P., Gakhar, L., Yu, L. & Artemyev, N. O. (2017). Unique structural features of the AIPL1-FKBP domain that support prenyl lipid binding and underlie protein malfunction in blindness. Proceedings of the National Academy of Sciences of the United States of America, 114(32), E6536-E6545. PMID: 28739921.

Yu, L., Yadav, R. P. & Artemyev, N. O. (2017). NMR resonance assignments of the FKBP domain of human aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) in complex with a farnesyl ligand. Biomolecular NMR assignments, 11(1), 111-115. PMID: 28236226.

Yadav, R. P., Artemyev, N. O. (2017). AIPL1: A specialized chaperone for the phototransduction effector. Cellular signalling, 40, 183-189. PMID: 28939106.

Egbert, J. R., Uliasz, T. F., Shuhaibar, L. C., Geerts, A., Wunder, F., Kleiman, R. J., Humphrey, J. M., Lampe, P. D., Artemyev, N. O., Rybalkin, S. D., Beavo, J. A., Movsesian, M. A. & Jaffe, L. A. (2016). Luteinizing Hormone Causes Phosphorylation and Activation of the cGMP Phosphodiesterase PDE5 in Rat Ovarian Follicles, Contributing, Together with PDE1 Activity, to the Resumption of Meiosis. Biology of reproduction, 94(5), 110. PMID: 27009040.

Gopalakrishna, K. N., Boyd, K., Yadav, R. P. & Artemyev, N. O. (2016). Aryl Hydrocarbon Receptor-interacting Protein-like 1 Is an Obligate Chaperone of Phosphodiesterase 6 and Is Assisted by the ?-Subunit of Its Client. Journal of biological chemistry, 291(31), 16282-16291. PMID: 27268253.

Majumder, A. A., Pahlberg, J. C., Muradov, H. A., Boyd, K. K., Sampath, A. P. & Artemyev, N. (2015). Exchange of cone for rod phosphodiesterase 6 catalytic subunits in rod photoreceptors mimics in part features of light adaptation. Journal of Neuroscience, 35(24), 9225–9235. PMID: 26085644.

Yadav, R. P., Majumder, A., Gakhar, L. & Artemyev, N. (2015). Extended conformation of the proline-rich domain of human aryl hydrocarbon receptor-interacting protein-like 1: Implications for retina disease. Journal of Neurochemistry, 135(1), 165–175. DOI: 10.1111/jnc.13223.

Cheguru, P., Majumder, A., Yadav, R., Gopalakrishna, K. N., Gakhar, L. & Artemyev, N. O. (2015). The solution structure of the transducin-a-uncoordinated 119 protein complex suggests occlusion of the Gß1?1-binding sites. The FEBS journal, 282(3), 550-61. PMID: 25425538.

Cheguru, P., Majumder, A. & Artemyev, N. O. (2015). Distinct patterns of compartmentalization and proteolytic stability of PDE6C mutants linked to achromatopsia. Molecular and cellular neurosciences, 64, 1-8. PMID: 25461672.

Kuburas, A., Thompson, S., Artemyev, N. O., Kardon, R. H. & Russo, A. F. (2014). Photophobia and abnormally sustained pupil responses in a mouse model of bradyopsia. Investigative ophthalmology & visual science, 55(10), 6878-85. PMID: 25257059.

Manes, G., Cheguru, P., Majumder, A., Bocquet, B., Senechal, A., Artemyev, N. O., Hamel, C. P. & Brabet, P. (2014). A Truncated Form of Rod Photoreceptor PDE6 beta-Subunit Causes Autosomal Dominant Congenital Stationary Night Blindness by Interfering with the Inhibitory Activity of the gamma-Subunit. PLoS One, 9(4), e95768. PMID: 24760071.

Artemyev, N. (2014). G protein-effector coupling in the vertebrate phototransduction cascade. In K. A. Martemyanov , A. P. Sampath (Eds.) G protein signaling mechanisms in the retina. pp. 49-66. New York: Springer.

Cheguru, P., Zhang, Z. & Artemyev, N. O. (2014). The GAFa domain of phosphodiesterase-6 contains a rod outer segment localization signal. J Neurochem, 129(2), 256-63. PMID: 24147783.

Liu, X., Kerov, V., Haeseleer, F., Majumder, A., Artemyev, N., Baker, S. A. & Lee, A. (2013). Dysregulation of Ca(v)1.4 channels disrupts the maturation of photoreceptor synaptic ribbons in congenital stationary night blindness type 2. Channels, 7(6), 514-23. PMID: 24064553.

Sinha, S., Majumder, A., Belcastro, M., Sokolov, M. & Artemyev, N. O. (2013). Expression and subcellular distribution of UNC119a, a protein partner of transducin alpha subunit in rod photoreceptors. Cell Signal, 25(1), 341-8. PMID: 23072788.

Majumder, A., Gopalakrishna, K. N., Cheguru, P., Gakhar, L. & Artemyev, N. O. (2013). Interaction of Aryl Hydrocarbon Receptor-interacting Protein-like 1 with the Farnesyl Moiety. J Bio Chem, 288(29), 21320-8. PMID: 23737531.

Majumder, A., Pahlberg, J., Boyd, K. K., Kerov, V., Kolandaivelu, S., Ramamurthy, V., Sampath, A. P. & Artemyev, N. O. (2013). Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells. Proc Natl Acad Sci USA, 110(30), 12468-73. PMID: 23836670.

Gopalakrishna, K. N., Boyd, K. K. & Artemyev, N. (2012). Comparative analysis of cone and rod transducins using chimeric Ga subunits. (Vols. 51). (8), pp. 1617-1624. Biochemistry (mosc). PMID: 22324825.

Muradov, H., Boyd, K. K., Kerov, V. & Artemyev, N. (2012). Atypical retinal degeneration 3 in mice is caused by defective PDE6B pre-mRNA splicing. Vision Res, 57, 1-8. PMID: 22326271.

Gopalakrishna, K. N., Doddapuneni, k., Boyd, K. K., Masuho, I., Martemyanov, K. A. & Artemyev, N. (2011). Interaction of transducin with uncoordinated 119 protein (UNC119): implications for the model of transducin trafficking in rod photoreceptors. (Vols. 286). (33), pp. 28954-28962. J. Biol. Chem.. PMID: 21712387.

Kerov, V., Artemyev, N. (2011). Diffusion and light-dependent compartmentalization of transducin. (Vols. 46). (1), pp. 340-346. Mol. Cell. Neurosci.. PMID: ISI:000286487800033.

Grau, T., Artemyev, N., Rosenburg, T., Dollfus, H., Haugen, O. H., Sener, E. C., Jurklies, B., Andreasson, S., Kernstock, C., Larson, M., Zrenner, E., Wissinger, B. & Kohl, S. (2011). Decreased catalytic activity and altered activation properties of PDE6C mutants associated with autosomal recessive achromatopsia. (Vols. 20). (4), pp. 719-730. Hum Mol Genet.. PMID: ISI:000286469400009.

Muradov, H., Boyd, K. K. & Artemyev, N. (2010). Rod phosphodiesterase-6 PDE6A and PDE6B subunits are enzymatically equivalent. (Vols. 285). (51), pp. 39828-39834. J. Biol. Chem.. PMID: ISI:000285185500027.

Zhang, Z., Artemyev, N. (2010). Determinants for phosphodiesterase 6 Inhibition by its gamma -subunit. (Vols. 49). (18), pp. 3862-3867. Biochemistry (Mosc). PMID: ISI:000277212400009.

Zhang, Z., Artemyev, N. (2010). Determinants for phosphodiesterase 6 inhibition by its ?-subunit. Biochemistry, 49(18), 3862–3867. PMID: 20397626.

Braun, J., Barren, B., Proft, J., Artemyev, N. & Braun, J. (2010). Association of the neuroprotective J proteins: cysteine string protein and Hsp40 following heat shock. Biochemistry and Cell Bilogy- Biochimie Et Biologie Cellulaire, 88(2), 397. PMID: ISI:000278015800040.

Barren, B., Gakhar, L., Muradov, H., Boyd, K., Ramaswamy, S. b. & Artemyev, N. (2009). Structural basis of phosphodiesterase 6 inhibition by the C-terminal region of the ?-subunit. EMBO Journal, 28(22), 3613–3622. PMID: 19798052.

Gibbs, S. J., Barren, B., Beck, K. E., Proft, J., Zhao, X., Noskova, T., Braun, A. P., Artemyev, N. & Braun, J. E. (2009). Hsp40 couples with the CSP? chaperone complex upon induction of the heat shock response.

Barren, B., Gakhar, L., Muradov, H., Boyd, K., Ramaswamy, S. & Artemyev, N. (2009). Structural basis of phosphodiesterase 6 Inhibition by the ?-subunit. (Vols. 28). pp. 3613-3622. EMBO J..

Gibbs, S. J., Barren, B., Beck, K. E., Proft, J., Zhao, X., Noskova, T., Braun, A. P., Artemyev, N. O. & Braun, J. E. (2009). Hsp40 couples with the CSPalpha chaperone complex upon induction of the heat shock response. PloS one, 4(2), e4595. PMID: 19242542.

Chang, B., Grau, T., Dangel, S., Hurd, R., Jurklies, B., Sener, E. C., Andreasson, S., Dollfus, H., Baumann, B., Bolz, S., Artemyev, N., Kohl, S., Heckenlively, J. & Wissinger, B. (2009). A homologous genetic basis of the murine cpfl1 mutant and human achromatopsia linked to mutations in the PDE6C gene.

Muradov, H., Boyd, K. K., Haeri, M., Kerov, V., Knox, B. E. & Artemyev, N. (2009). Characterization of human cone phosphodiesterase-6 ectopically expressed in Xenopus laevis rods. (Vols. 284). pp. 32662-32669. J. Biol. Chem..

Muradov, K. G., Boyd, K. K., Kerov, V. & Artemyev, N. (2008). Unique transducins expressed in long and short photoreceptors of lamprey Petromyzon marinus.

Song, J., Guo, L. W., Muradov, H., Artemyev, N., Ruoho, A. E. & Markley, J. L. (2008). Intrinsically disordered ?-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure.

Artemyev, N. O. (2008). Phosphodiesterase 6A, cGMP-specific rod beta. In UCSD Nature Molecule Pages. UCSD Signaling Gateway. DOI: 1038/mp.a001755.01.

Song, J., Guo, L., Muradov, H., Artemyev, N., Ruoho, A. & Markley, J. (2008). Intrinsically disordered ?-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure. Proceedings of the National Academy of Sciences of the United States of America, 105(5), 1505–1510. PMID: 18230733.

Artemyev, N. (2008). Light-dependent compartmentalization of transducin in rod photoreceptors.

Ahmad, S. c., Natochin, M., Artemyev, N. & O’Tousa, J. d. (2007). The Drosophila rhodopsin cytoplasmic tail domain is required for maintenance of rhabdomere structure. FASEB Journal, 21(2), 449–455. DOI: 10.1096/fj.06-6530com.

, Rubin, W. W., Natochin, M., Melling, N., Burns, M. E. & Artemyev, N. (2007). N-terminal fatty acylation of transducin profoundly influences its localization and the kinetics of photoresponse in rods. (Vols. 27). pp. 10270-10277. J. Neurosci..

Muradov, K. G., Boyd, K. K., kerov, V. & Artemyev, N. (2007). PDE6 in lamprey Petromyzon marinus: implications for the evolution of the visual effector in vertebrates.

Natochin, M., Muradov, K. G. & Artemyev, N. (2007). Function and regulation of PDE6. In Fliesler , Kisselev (Eds.) Signal Transduction in the Retina. Taylor & Francis/CRC Press.

Barren, B., Artemyev, N. (2007). Mechanisms of dominant negative G-protein ?-subunits. (Vols. 85). pp. 3505-3514. J. Neurosci. Res..

Ahmad, S. T., Natochin, M., Artemyev, N. & O'Tousa, J. E. (2007). Drosophila rhodopsin cytoplasmic tail domain is required for maintenance of rhabdomere structure. (Vols. 21). pp. 449-455. FASEB J..

Barren, B., Artemyev, N. b. (2007). Mechanisms of dominant negative G-protein a subunits. Journal of Neuroscience Research, 85(16), 3505–3514. DOI: 10.1002/jnr.21414.

Muradov, K. G., Boyd, K. K. & Artemyev, N. (2006). Analysis of PDE6 function using chimeric PDE5/6 catalytic domains.

Natochin, M., Barren, B. & Artemyev, N. b. (2006). Dominant negative mutants of transducin-a that block activated receptor. Biochemistry, 45(20), 6488–6494. PMID: 16700559.

Barren, B. & Artemyev, N. (2006). "Mutation R238/E in transducin-? yields a GTPase and effector-deficient, but not dominant-negative G-protein a-subunit".

Natochin, M., Rubin, W. W., Kerov, V., Reh, R., Chen, D., Lem, J., Chen, C. K., Hurley, J. B., Burns, M. E. & Artemyev, N. (2006). Phototransduction in a transgenic mouse model of Nougaret night blindness. (Vols. 26). pp. 6863-6872. J. Neurosci..

Ahmad, S. T., Natochin, M., Barren, B., Artemyev, N. & O'Tousa, J. E. (2006). Heterologous expression of bovine rhodopsin in drosophila photoreceptor cells.

Natochin, M., Barren, B., Ahmad, S. T., O'Tousa, J. E. & Artemyev, N. (2006). Probing rhodopsin-transducin interaction using Drosophila Rh1-bovine rhodopsin chimeras.

Natochin, M., Barren, B. & Artemyev, N. (2006). "Dominant negative mutants of transducin-a that block activated receptor".

Barren, B., Natochin, M. & Artemyev, N. O. (2006). Mutation R238E in transducin-alpha yields a GTPase and effector-deficient, but not dominant-negative, G-protein alpha-subunit. Molecular vision, 12, 492-8. PMID: 16735989.

Guo, L. W., Muradov, H., Hajipour, A. R., Sievert, M. K., Artemyev, N. & Ruoho, A. E. (2006). The inhibitory gamma subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure. (Vols. 281). pp. 15412-15422. J. Biol. Chem..

Guo, L. d., Muradov, H., Hajipour, A. c., Sievert, M., Artemyev, N. & Ruoho, A. (2006). The inhibitory ? subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure. Journal of Biological Chemistry, 281(22), 15412–15422. DOI: 10.1074/jbc.M600595200.

Kerov, V., Natochin, M. & Artemyev, N. (2005). Interaction of transducin-a with LGN, a G-protein modulator expressed in photoreceptor cells. Molecular and Cellular Neuroscience, 28(3), 485–495. DOI: 10.1016/j.mcn.2004.10.010.

Guo, L., Grant, J., Hajipourt, A. c., Muradov, H., Arbabian, M., Artemyev, N. & Ruoho, A. d. (2005). Asymmetric interaction between rod cyclic GMP phosphodiesterase ? subunits and aß subunits. Journal of Biological Chemistry, 280(13), 12585–12592. DOI: 10.1074/jbc.M410380200.

Kero, V., Natochin, M. & Artemyev, N. (2005). Interaction of transducin-? with LGN, a G-protein modulator expressed in photoreceptor cells.

Natochin, M., Campbell, T., Barren, B., Miller, L., Hameed, S., Artemyev, N. & Braun, J. c. (2005). Characterization of the Gas regulator cysteine string protein. Journal of Biological Chemistry, 280(34), 30236–30241. DOI: 10.1074/jbc.M500722200.

Kerov, V., Chen, D., Moussaif, M., Chen, Y. J., Chen, C. K. & Artemyev, N. (2005). Transducin activation state controls its light-dependent translocation in rod photoreceptors. (Vols. 280). pp. 41069-41076. J. Biol. Chem..

Muradov, K. G., Boyd, K. K. & Artemyev, N. O. (2005). Analysis of dimerization determinants of PDE6 catalytic subunits. Methods in molecular biology (Clifton, N.J.), 307, 263–275.

Muradov, K. G., Boyd, K. K. & Artemyev, N. (2005). Analysis of dimerization determinants of the PDE6 catalytic subunits.

Artemyev, N. (2005). Interactions between catalytic and inhibitory subunits of PDE6.

Muradov, H., Boyd, K. & Artemyev, N. (2004). Structural determinants of the PDE6 GAF a domain for binding the inhibitory ?-subunit and noncatalytic cGMP. Vision Research, 44(21), 2437–2444. DOI: 10.1016/j.visres.2004.05.013.

Artemyev, N., Kerov, v. & Natochin, M. (2004). LGN, a transducin-? binding protein, is present in the inner segments and synaptic terminals of photoreceptor cells.

Muradov, K. G., Boyd, K. K., Martinez, S. E., Beavo, J. A. & Artemyev, N. (2003). The GAFa domains of rod cGMP-phosphodiesterase 6 determine the selectivity of the enzyme dimerization. (Vols. 278). pp. 10594-10601. J. Biol. Chem..

Natochin, M., Gasimov, K., Moussaif, M. & Artemyev, N. (2003). Rhodopsin determinants for transducin activation: A gain-of-function approach. (Vols. 278). pp. 37574-37581. J. Biol. Chem..

Natochin, M., Artemyev, N. (2003). A point mutation uncouples transducin-a from the photoreceptor RGS and effector proteins. Journal of Neurochemistry, 87(5), 1262–1271. PMID: 14622106.

Muradov, K., Granovsky, A. & Artemyev, N. (2003). Mutation in rod PDE6 linked to congenital stationary night blindness impairs the enzyme inhibition by its ?-subunit. Biochemistry, 42(11), 3305–3310. DOI: 10.1021/bi027095x.

Natochin, M., Artemyev, N. (2003). A point mutation uncouples transducin-? from the photoreceptor RGS and effector proteins. (Vols. 87). pp. 1262-1271. J. Neurochem..

Natochin, M., Gasimov, K. & Artemyev, N. (2002). A GPR-protein interaction surface of Gia: Implications for the mechanism of GDP-release inhibition. Biochemistry, 41(1), 258–265. PMID: 11772024.

Granovsky, A. E., Artemyev, N. (2002). Assays of G protein – cGMP phosphodiesterase interactions.

Muradov, K., Granovsky, A., Schey, K. & Artemyev, N. (2002). Direct interaction of the inhibitory ?-subunit of rod cGMP phosphodiesterase (PDE6) with the PDE6 GAFa domains. Biochemistry, 41(12), 3884–3890. PMID: 11900530.

Granovsky, A. E., Artemyev, N. (2001). Partial reconstitution of photoreceptor cGMP phosphodiesterase characteristics in cGMP phosphodiesterase-5. (Vols. 276). pp. 21698-21703. J. Biol. Chem..

Granovsky, A., Artemyev, N. (2001). A conformational switch in the inhibitory ?-subunit of PDE6 upon enzyme activation by transducin. Biochemistry, 40(44), 13209–13215. PMID: 11683629.

Natochin, M., Gasimov, K. & Artemyev, N. (2001). Inhibition of GDP/GTP exchange on Ga subunits by proteins containing G-protein regulatory motifs. Biochemistry, 40(17), 5322–5328. PMID: 11318657.

Granovsky, A. E., Artemyev, N. O. (2001). A conformational switch in the inhibitory gamma-subunit of PDE6 upon enzyme activation by transducin. Biochemistry, 40(44), 13209-15. PMID: 11683629.

Natochin, M., Moussaif, M. & Artemyev, N. (2001). Probing the mechanism of rhodopsin-catalyzed transducin activation. (Vols. 77). pp. 202-210. J. Neurochem..

Granovsky, A. E., Artemyev, N. (2000). Identification of the ?-subunit interacting residues on photoreceptor cGMP phosphodiesterase, PDE6?’.

Granovsky, A. E., Muradov, K. G. & Artemyev, N. (2000). Inhibition of photoreceptor cGMP phoshodiesterase by its ?-subunit.

Granovsky, A., Artemyev, N. (2000). Identification of the ? subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6a'. Journal of Biological Chemistry, 275(52), 41258–41262. DOI: 10.1074/jbc.M008094200.

Granovsky, A., Muradov, K. & Artemyev, N. (2000). Inhibition of photoreceptor cGMP phosphodiesterase by its ? subunit. Methods in Enzymology, 315, 635–646.

Muradov, K., Artemyev, N. (2000). Loss of the effector function in a transducin-a mutant associated with Nougaret night blindness. Journal of Biological Chemistry, 275(10), 6969–6974. DOI: 10.1074/jbc.275.10.6969.

Natochin, M., Artemyev, N. (2000). Mutational analysis of functional interfaces of transducin.

Artemyev, N., Muradov, K. G. (2000). Loss of the effector function in a transducin-? mutant associated with Nougaret night blindness.

Muradov, K., Artemyev, N. (2000). Coupling between the N- and C-terminal domains influences transducin-a intrinsic GDP/GTP exchange. Biochemistry, 39(14), 3937–3942. PMID: 10747781.

Natochin, M., Lester, B., Peterson, Y., Bernard, M., Lanier, S. & Artemyev, N. (2000). AGS3 inhibits GDP dissociation from Ga subunits of the Gi family and rhodopsin-dependent activation of transducin. Journal of Biological Chemistry, 275(52), 40981–40985. DOI: 10.1074/jbc.M006478200.

Kanzaki, M., Watson, R., Artemyev, N. & Pessin, J. (2000). The trimeric GTP-binding protein (G(q)/G11) a subunit is required for insulin-stimulated GLUT4 translocation in 3T3L1 adipocytes. Journal of Biological Chemistry, 275(10), 7167–7175. DOI: 10.1074/jbc.275.10.7167.

Natochin, M., Lester, B. R., Peterson, Y. K., Bernard, M. L., Lanier, S. M. & Artemyev, N. (2000). AGS3 inhibits GDP dissociation from G? subunits of Gi family and rhodopsin-dependent activation of transducin.

Natochin, M., Muradov, K., McEntaffer, R. & Artemyev, N. (2000). Rhodopsin recognition by mutant G(s)a containing C-terminal residues of transducin. Journal of Biological Chemistry, 275(4), 2669–2675. DOI: 10.1074/jbc.275.4.2669.

Natochin, M., Granovsky, A., Muradov, K. & Artemyev, N. (1999). Roles of the transducin a-subunit a4-helix/a4-ß6 loop in the receptor and effector interactions. Journal of Biological Chemistry, 274(12), 7865–7869. DOI: 10.1074/jbc.274.12.7865.

D’Amours, M. R., Granovsky, A. E., Artemyev, N. & Cote, R. H. (1999). The potency and mechanism of action of E4021, a PDE5-selective inhibitor, on photoreceptor phosphodoesterase depend on the state of activation of the enzyme.

McEntaffer, R., Natochin, M. & Artemyev, N. (1999). Modulation of transducin GTPase activity by chimeric RGS16 and RGS9 regulators of G protein signaling and the effector molecule.

D’Amours, M., Granovsky, A., Artemyev, N. & Cote, R. c. (1999). Potency and mechanism of action of E4021, a type 5 phosphodiesterase isozyme-selective inhibitor, on the photoreceptor phosphodiesterase depend on the state of activation of the enzyme. Molecular Pharmacology, 55(3), 508–514.

Natochin, M., Granovsky, A. E., Muradov, K. G. & Artemyev, N. (1999). . Roles of the transducin ?4-helix/?4-?6 loop in the receptor and effector interactions.

Artemyev, N., Natochin, M. (1998). Substitution of transducin Ser202 by Asp abolishes G-protein/RGS interaction.

Granovsky, A., Natochin, M., McEntaffer, R., Haik, T., Francis, S., Corbin, J. & Artemyev, N. (1998). Probing domain functions of chimeric PDE6a’/PDE5 cGMP- phosphodiesterase. Journal of Biological Chemistry, 273(38), 24485–24490. DOI: 10.1074/jbc.273.38.24485.

Artemyev, N., Arshavsky, V. & Cote, R. (1998). Photoreceptor phosphodiesterase: Interaction of inhibitory ? subunit and cyclic GMP with specific binding sites on catalytic subunits. Methods: A Companion to Methods in Enzymology, 14(1), 93–104. DOI: 10.1006/meth.1997.0568.

Granovsky, A. E., McEntaffer, R. & Artemyev, N. O. (1998). Probing functional interfaces of rod PDE gamma-subunit using scanning fluorescent labeling. Cell biochemistry and biophysics, 28(2-3), 115-33. PMID: 9515163.

Natochin, M., McEntaffer, R. & Artemyev, N. (1998). Mutational analysis of the Asn residue essential for RGS protein binding to G-proteins.

Natochin, M., Granovsky, A. E. & Artemyev, N. O. (1998). Identification of Effector Residues on Photoreceptor G Protein, Transducin. Journal of Biological Chemistry, 273(34), 21808–21815. DOI: 10.1074/jbc.273.34.21808.

Natochin, M., Artemyev, N. (1998). A single mutation Asp229 ? Ser confers upon G(s)a the ability to interact with regulators of G protein signaling. Biochemistry, 37(39), 13776–13780. DOI: 10.1021/bi981155a.

Granovsky, A. E., Natochin, M. & Artemyev, N. O. (1997). The gamma Subunit of Rod cGMP-Phosphodiesterase Blocks the Enzyme Catalytic Site. Journal of Biological Chemistry, 272(18), 11686?11689. DOI: 10.1074/jbc.272.18.11686.

Natochin, M., Granovsky, A. E. & Artemyev, N. (1997). Regulation of transducin GTPase activity by human retinal RGS.

Artemyev, N. (1997). Binding of transducin to light-activated rhodopsin prevents transducin interaction with the rod cGMP phosphodiesterase ?-subunit. Biochemistry, 36(14), 4188–4193. DOI: 10.1021/bi963002y.

Natochin, M., Lipkin, V. & Artemyev, N. (1997). Interaction of human retinal RGS with G-protein a-subunits. FEBS Letters, 411(2-3), 179–182. PMID: 9271201.

Artemyev, N. (1997). Binding of transducin to light-activated rhodopsin prevents transducin interaction with the rod cGMP-phosphodiesterase ?-subunit.

Mazzoni, M. R., Artemyev, N. & Hamm, H. E. (1996). Proteolytic fragmentation for epitope mapping.

Natochin, M., Artemyev, N. (1996). An interface of interaction between photoreceptor cGMP phosphodiesterase catalytic subunits and inhibitory ? subunits. Journal of Biological Chemistry, 271(33), 19964–19969. DOI: 10.1074/jbc.271.33.19964.

Artemyev, N. c., Natochin, M., Busman, M., Schey, K. & Hamm, H. (1996). Mechanism of photoreceptor cGMP phosphodiesterase inhibition by its ?-subunits. Proceedings of the National Academy of Sciences of the United States of America, 93(11), 5407–5412.

Artemyev, N. O., Surendran, R., Lee, J. C. & Hamm, H. E. (1996). Subunit Structure of Rod cGMP-Phosphodiesterase. Journal of Biological Chemistry, 271(41), 25382–25388. DOI: 10.1074/jbc.271.41.25382.

Artemyev, N., Natochin, m., Busman, M., Schey, K. L. & Hamm, H. E. (1996). Mechanism of photoreceptor PDE inhibition by its ?-subunits.

Artemyev, N. O., Natochin, M., Busman, M., Schey, K. L. & Hamm, H. E. (1996). Mechanism of photoreceptor cGMP phosphodiesterase inhibition by its gamma-subunits. Proceedings of the National Academy of Sciences of the United States of America, 93(11), 5407-12. PMID: 8643588.

Artemyev, N. (1996). An interface of interaction between photoreceptor cGMP phosphodiesterase catalytic subunits and inhibitory ?-subunits.

Skiba, N., Artemyev, N. & Hamm, H. (1995). The carboxyl terminus of the ?-subunit of rod cGMP phosphodiesterase contains distinct sites of interaction with the enzyme catalytic subunits and the a-subunit of transducin. Journal of Biological Chemistry, 270(22), 13210–13215.

Slepak, V., Artemyev, N., Zhu, Y., Dumke, C., Sabacan, L., Sondek, J., Hamm, H., Bownds, M. & Arshavsky, V. (1995). An effector site that stimulates G-protein GTPase in photoreceptors. Journal of Biological Chemistry, 270(24), 14319–14324. DOI: 10.1074/jbc.270.24.14319.

Arshavsky, V. Y., Dumke, C. L., Artemyev, N., Skiba, N. P., Hamm, H. E. & Bownds, M. D. (1994). Transducin GTPase activity in bovine rod outer segments is accelerated by the ? subunit of cGMP phosphodiesterase.

Artemyev, N. O., Hamm, H. E. (1994). Probing G-protein function. (Vols. 1). (11), pp. 752-4. Nature structural biology. PMID: 7634081.

Rarick, H. M., Artemyev, N., Mills, J. S., Skiba, N. P. & Hamm, H. E. (1994). Specific peptide probes for G-protein interaction with effectors.

Arshavsky, V. d., Dumke, C., Zhu, Y., Artemyev, N., Skiba, N., Hamm, H. & Bownds, M. b. (1994). Regulation of transducin GTPase activity in bovine rod outer segments. Journal of Biological Chemistry, 269(31), 19882–19887. PMID: 8051070.

Artemyev, N., Mills, J., Thornburg, K., Knapp, D., Schey, K. & Hamm, H. c. (1993). A site on transducin alpha-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhibitory subunit. Journal of Biological Chemistry, 268(31), 23611?23615. PMID: 8226888.

Artemyev, N., Skiba, N., Mills, J. & Hamm, H. (1993). Rod cGMP-Phosphodiesterase ?-Subunit: Structure-Function Relationships. Methods, 5(3), 220–228. DOI: 10.1006/meth.1993.1027.

Artemyev, N., Hamm, H. E., Mills, J. S., Skiba, N. P., Rarick, H. M., Lambert, C. & Dratz, E. (1992). Sites and mechanisms of interaction of rod G protein with rhodopsin and cGMP phosphodiesterase.

Rarick, H., Artemyev, N. & Hamm, H. (1992). A site on rod G protein a subunit that mediates effector activation. Science, 256(5059), 1031–1033.

Artemyev, N., Rarick, H. M. & Hamm, H. E. (1992). A site on rod G-protein ? subunit that mediates effector activation.

Artemyev, N. O., Rarick, H. M., Mills, J. S., Skiba, N. P. & Hamm, H. E. (1992). Sites of interaction between rod G-protein alpha-subunit and cGMP-phosphodiesterase gamma-subunit. Implications for the phosphodiesterase activation mechanism. The Journal of biological chemistry, 267(35), 25067-72. PMID: 1334079.

Artemyev, N., Hamm, H. (1992). Two-site high-affinity interaction between inhibitory and catalytic subunits of rod cyclic GMP phosphodiesterase. Biochemical Journal, 283(1), 273–279.

Artemyev, N., Garnovskaya, M. N., Ostapenko, I. A. & Ritchkova, M. P. (1991). A native model for structure-and-function studies of photoreceptor cGMP phosphodiesterase subunits.

Artemyev, N., Garnovskaya, M. N., Dumler, I. L. & Etingof, R. N. (1990). Is the ?-subunit of cGMP phosphodiesterase of rod outer segments unique? A new protein with similar properties.

Artemyev, N., Dumler, I. L., Chernova, T. D. & Etingof, R. N. (1988). Different functional sites of the cyclic GMP phosphodiesterase ?-subunit molecule: estimation using monoclonal antibodies.

Artemyev, N., Dumler, I. L., Muradov, K. G., Lipkin, V. M. & Etingof, R. N. (1988). Localization of functional regions in the ?-subunit of 3’,5’-cGMP phosphodiesterase from bovine retina photoreceptor membranes.

Artemyev, N., Shelkovnikov, S. A., Starshinova, L. A., Garnovskaya, M. N., Etingof, R. N. & Dumler, I. L. (1988). The peptide-activator of cyclic nucleotide phosphodiesterase: effect on muscle contractile activity.

Artemyev, N., Dumler, I. L., Muradov, K. G., Lipkin, V. M. & Etingof, R. N. (1988). The role of functional domains of the cGMP phosphodiesterase ?-subunit in the visual transduction mechanism.

Artemyev, N., Garnovskaya, M. & Dumler, I. (1988). Cyclic nucleotide phosphodiesterase from uterine tissue: Purification and some properties. Biokhimiya, 53(8), 1304–1309.

Lipkin, V., Dumler, I., Muradov, K., Artemyev, N. & Etingof, R. (1988). Active sites of the cyclic GMP phosphodiesterase ?-subunit of retinal rod outer segments. FEBS Letters, 234(2), 287–290. PMID: 2455657.

Artemyev, N., Lipkin, V. M., Dumler, I. L., Muradov, K. G. & Etingof, P. N. (1988). Active sites of the cyclic GMP phosphodiesterase ?-subunit of retinal rod outer segments.

Artemyev, N., Garnovskaya, M. N. & Dumler, I. L. (1988). Cyclic nucleotide phosphodiesterase from uterine tissue: purification and properties.

Artemyev, N., Etingof, R. (1987). On 3’,5’-cGMP phosphodiesterase methylation in retinal photoreceptor membranes. Biokhimiya, 52(1), 154–159.

Artemyev, N., Garnovskaya, M. N., Dumler, I. L. & Etingof, R. N. (1987). Detection of the cyclic nucleotide phosphodiesterase activator peptide in uterine tissue.

Artemyev, N., Etingof, R. N. (1987). 3’,5’-cGMP-phosphodiesterase methylation in retinal photoreceptor membranes.

Dumler, I., Garnovskaya, M., Artemyev, N. & Etingof, R. (1984). Comparative immunochemical analysis of various phosphodiesterases of cyclic nucleotides. Biokhimiya, 49(10), 1727–1732.

Artemyev, N., Dumler, I. L., Garnovskaya, M. N. & Etingof, R. N. (1984). Comparative immunochemical analysis of cyclic nucleotides phosphodiesterases.