One of the services of the Proteomics Facility is tryptic digestion of proteins in gel, in PVDF, and in solution. This is a critical step in protein identification analyses and we understand that some researchers would prefer to do this step themselves. We have developed procedures and practices that have been successful for us and are presenting them here. We have utilized a variety of sources to develop these procedures.
- Procedure for In Gel Tryptic Digest for Coomassie-stained Gels
- Procedure for Tryptic Digest for Silver Stained Gels
- Procedure for Reducution and Alkylation