The Metabolomics Core provides investigators with metabolite profiling and isotope tracing using high-resolution mass spectrometry (MS) following either gas chromatography (GC) or liquid chromatography (LC).  

The semi-targeted high-resolution GC-MS protocol identifies and measures more than 100 metabolites (and counting). These include TCA cycle and glycolytic/gluconeogenic intermediates as well as amino acids, sugars, neurotransmitters, and fatty acids.

The high-resolution LC-MS analysis focuses on compounds not amenable to the high temperatures of gas chromatography such as AMP, ADP, ATP, and other redox metabolites, coenzymes, nucleotides, and complex lipids.

Acknowledgements

We are thankful for the generous support of the Fraternal Order of Eagles Diabetes Research Center (FOEDRC).