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Budd A. Tucker, PhD

Portrait
Howard Ruby Chair for Regenerative Ophthalmology
Professor
Professor of Ophthalmology and Visual Sciences

Contact Information

Primary Office
4111 MERF
Iowa City, IA 52242
319-353-4488

Education

BA, Biology, Sir Wilfred Grenfell College, Memorial University of Newfoundland
PhD, Neuroscience, Memorial University of Newfoundland
Fellow, Ophthalmology, Harvard Medical School

Education/Training Program Affiliations

Interdisciplinary Graduate Program in Molecular Medicine, Interdisciplinary Graduate Program in Neuroscience, Interdisciplinary Graduate Program in Translational Biomedicine

Center, Program and Institute Affiliations

Institute for Vision Research

Research Summary

During my career I have had extensive experience and published several manuscripts/scientific abstracts relating to stem cell production, culture, isolation, differentiation and transplantation (via both cell injection and polymer scaffold mediated delivery).

Publications

Shrestha, A., Allen, B. N., Wiley, L. A., Tucker, B. A. & Worthington, K. S. (2020). Development of High-Resolution Three-Dimensional-Printed Extracellular Matrix Scaffolds and Their Compatibility with Pluripotent Stem Cells and Early Retinal Cells. Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 36(1), 42-55. PMID: 31414943.

Voigt, A. P., Mulfaul, K., Mullin, N. K., Flamme-Wiese, M. J., Giacalone, J. C., Stone, E. M., Tucker, B. A., Scheetz, T. E. & Mullins, R. F. (2019). Single-cell transcriptomics of the human retinal pigment epithelium and choroid in health and macular degeneration. Proceedings of the National Academy of Sciences of the United States of America, 116(48), 24100-24107. PMID: 31712411.

Wiley, L. A., Binkley, E. M., DeLuca, A. P., Workalemahu, G., Tatro, N. J., Luse, M. A., Kennedy, E. L., Folk, J. C., Scheetz, T. E., Ballas, Z. K., Tucker, B. A., Mullins, R. F., Han, I. C. & Stone, E. M. (2019). AUTOIMMUNE RETINOPATHY MIMICKING HERITABLE RETINAL DEGENERATION IN A PATIENT WITH COMMON VARIABLE IMMUNE DEFICIENCY. Retinal cases & brief reports. PMID: 31764884.

Han, I. C., Burnight, E. R., Ulferts, M. J., Worthington, K. S., Russell, S. R., Sohn, E. H., Mullins, R. F., Stone, E. M., Tucker, B. A. & Wiley, L. A. (2019). Helper-Dependent Adenovirus Transduces the Human and Rat Retina but Elicits an Inflammatory Reaction When Delivered Subretinally in Rats. Human gene therapy, 30(11), 1371-1384. PMID: 31456426.

Thompson, J. R., Worthington, K. S., Green, B. J., Mullin, N. K., Jiao, C., Kaalberg, E. E., Wiley, L. A., Han, I. C., Russell, S. R., Sohn, E. H., Guymon, C. A., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2019). Two-photon polymerized poly(caprolactone) retinal cell delivery scaffolds and their systemic and retinal biocompatibility. Acta biomaterialia, 94, 204-218. PMID: 31055121.

Scruggs, B. A., Jiao, C., Cranston, C. M., Kaalberg, E., Wang, K., Russell, S. R., Wiley, L. A., Mullins, R. F., Stone, E. M., Tucker, B. A. & Sohn, E. H. (2019). Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell-Based Treatments. Stem cells translational medicine, 8(8), 797-809. PMID: 31004408.

Voigt, A. P., Whitmore, S. S., Flamme-Wiese, M. J., Riker, M. J., Wiley, L. A., Tucker, B. A., Stone, E. M., Mullins, R. F. & Scheetz, T. E. (2019). Molecular characterization of foveal versus peripheral human retina by single-cell RNA sequencing. Experimental eye research, 184, 234-242. PMID: 31075224.

Sohn, E. H., Flamme-Wiese, M. J., Whitmore, S. S., Workalemahu, G., Marneros, A. G., Boese, E. A., Kwon, Y. H., Wang, K., Abramoff, M. D., Tucker, B. A., Stone, E. M. & Mullins, R. F. (2019). Choriocapillaris Degeneration in Geographic Atrophy. The American journal of pathology, 189(7), 1473-1480. PMID: 31051169.

Tyler, S. R., Rotti, P. G., Sun, X., Yi, Y., Xie, W., Winter, M. C., Flamme-Wiese, M. J., Tucker, B. A., Mullins, R. F., Norris, A. W. & Engelhardt, J. F. (2019). PyMINEr Finds Gene and Autocrine-Paracrine Networks from Human Islet scRNA-Seq. Cell reports, 26(7), 1951-1964.e8. PMID: 30759402.

Worthington, K. S., Do, A. V., Smith, R., Tucker, B. A. & Salem, A. K. (2019). Two-Photon Polymerization as a Tool for Studying 3D Printed Topography-Induced Stem Cell Fate. Macromolecular bioscience, 19(2), e1800370. PMID: 30430755.

Han, I. C., Whitmore, S. S., Critser, D. B., Lee, S. Y., DeLuca, A. P., Daggett, H. T., Affatigato, L. M., Mullins, R. F., Tucker, B. A., Drack, A. V. & Stone, E. M. (2019). Wide-Field Swept-Source OCT and Angiography in X-Linked Retinoschisis. Ophthalmology. Retina, 3(2), 178-185. PMID: 31014769.

Zhang, Q., Giacalone, J. C., Searby, C., Stone, E. M., Tucker, B. A. & Sheffield, V. C. (2019). Disruption of RPGR protein interaction network is the common feature of RPGR missense variations that cause XLRP. Proceedings of the National Academy of Sciences of the United States of America, 116(4), 1353-1360. PMID: 30622176.

Wiley, L. A., Kaalberg, E. E., Jiao, C., Mullins, R. F., Stone, E. M., Tucker, B. & Han, I. (2019). Characterizing the immune response to helper-dependent adenovirus in the rat retina. (Vols. 60). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Mullins, R. F., Sohn, E. H., Flamme-Wiese, M. J., Luse, M. A., Reutzel, A. J., Tucker, B. & Stone, E. M. (2019). Choroidal vascular changes in early and advanced AMD: relationship with ARMS2 genotype. (Vols. 60). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Mulfaul, K., Giacalone, J. C., Chirco, K. R., Zeng, S., Songstad, A. E., Ochoa, D., Andorf, J. L., Daggett, H. T., Stone, E. M., Tucker, B. & Mullins, R. F. (2019). Choroidal Endothelial cells synthesize CFH locally to protect against complement mediated injury. (Vols. 60). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Bohrer, L. R., Cooke, J. A., Shrestha, A., Burnight, E. R., Anfinson, K. R., Collins, M. M., Mullins, R. F., Stone, E. M., Worthington, K. S., Wiley, L. A. & Tucker, B. (2019). Generation of rod- vs. cone-dominant patient-derived 3D retinal grafts for the treatment of retinal degenerative blindness. (Vols. 60). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Bohrer, L. R., Wiley, L. A., Burnight, E. R., Cooke, J. A., Giacalone, J. C., Anfinson, K. R., Andorf, J. L., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2019). Correction of NR2E3 Associated Enhanced S-cone Syndrome Patient-specific iPSCs using CRISPR-Cas9. GENES, 10(4). DOI: 10.3390/genes10040278.

Cooke, J. A., Cranston, C. M., Kennedy, E. L., Deluca, A., Whitmore, S. S., Scheetz, T. E., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2019). Variation in retinal differentiation capacity between patient derived hiPSC lines. Variation in retinal differentiation capacity between patient derived hiPSC lines. (Vols. 60). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Fingert, J. H., van der Heide, Carly,, Cooke, J. A., Roos, B. R., Jain, A., Rodine, K., Mullins, R. F. & Tucker, B. (2019). Clinical, molecular genetic, and functional analysis of a pedigree with a novel dominant optic atrophy mutation. (Vols. 60). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Giacalone, J. C., Andorf, J. L., Zhang, Q., Burnight, E. R., Ochoa, D., Reutzel, A. J., Collins, M. M., Sheffield, V. C., Mullins, R. F., Han, I. C., Stone, E. M. & Tucker, B. A. (2019). Development of a Molecularly Stable Gene Therapy Vector for the Treatment of RPGR-Associated X-Linked Retinitis Pigmentosa. HUMAN GENE THERAPY, 30(8), 967-974. DOI: 10.1089/hum.2018.244.

Giacalone, J. C., Miller, M. J., Workalemahu, G., Reutzel, A. J., Ochoa, D., Whitmore, S. S., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2019). Generation of an immortalized human choroid endothelial cell line (iChEC-1) using an endothelial cell specific promoter. MICROVASCULAR RESEARCH, 123, 50-57. DOI: 10.1016/j.mvr.2018.12.002.

Gibson-Corley, K. N., Cranston, C., Anfinson, K., Kaalberg, E., Han, I., Slusarski, D. C., Goeken, A., Businga, T., Brown, H., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2019). Development of gene augmentation strategy for the treatment of MAK associated Retinitis Pigmentosa. (Vols. 33). FASEB JOURNAL.

Miller, M., Fick, J., Hoffmann, J. M., Penticoff, J. A., Mullins, R. F., Stone, E. M. & Tucker, B. (2019). North Carolina Macular Dystrophy: Causes and Consequences of Gene Dysregulation. (Vols. 60). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Do, A. V., Worthington, K. S., Tucker, B. A. & Salem, A. K. (2018). Controlled drug delivery from 3D printed two-photon polymerized poly(ethylene glycol) dimethacrylate devices. International journal of pharmaceutics, 552(1-2), 217-224. PMID: 30268853.

Cheng, J., Sohn, E. H., Jiao, C., Adler, K. L., Kaalberg, E. E., Russell, S. R., Mullins, R. F., Stone, E. M., Tucker, B. A. & Han, I. C. (2018). Correlation of Optical Coherence Tomography and Retinal Histology in Normal and Pro23His Retinal Degeneration Pig. Translational vision science & technology, 7(6), 18. PMID: 30519502.

Chirco, K. R., Flamme-Wiese, M. J., Wiley, J. S., Potempa, L. A., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2018). Evaluation of serum and ocular levels of membrane attack complex and C-reactive protein in CFH-genotyped human donors. Eye (London, England), 32(11), 1740-1742. PMID: 30013157.

Green, B. J., Worthington, K. S., Thompson, J. R., Bunn, S. J., Rethwisch, M., Kaalberg, E. E., Jiao, C., Wiley, L. A., Mullins, R. F., Stone, E. M., Sohn, E. H., Tucker, B. A. & Guymon, C. A. (2018). Effect of Molecular Weight and Functionality on Acrylated Poly(caprolactone) for Stereolithography and Biomedical Applications. Biomacromolecules, 19(9), 3682-3692. PMID: 30044915.

Zeng, S., Wen, K. K., Workalemahu, G., Sohn, E. H., Wu, M., Chirco, K. R., Flamme-Wiese, M. J., Liu, X., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2018). Imidazole Compounds for Protecting Choroidal Endothelial Cells from Complement Injury. Scientific reports, 8(1), 13387. PMID: 30190604.

Wagoner, M. D., Bohrer, L. R., Aldrich, B. T., Greiner, M. A., Mullins, R. F., Worthington, K. S., Tucker, B. A. & Wiley, L. A. (2018). Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biology open, 7(5). PMID: 29685994.

Wiley, L. A., Burnight, E. R., Kaalberg, E. E., Jiao, C., Riker, M. J., Halder, J. A., Luse, M. A., Han, I. C., Russell, S. R., Sohn, E. H., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2018). Assessment of Adeno-Associated Virus Serotype Tropism in Human Retinal Explants. Human gene therapy, 29(4), 424-436. PMID: 29160116.

Burnight, E. R., Giacalone, J. C., Cooke, J. A., Thompson, J. R., Bohrer, L. R., Chirco, K. R., Drack, A. V., Fingert, J. H., Worthington, K. S., Wiley, L. A., Mullins, R. F., Stone, E. M. & Tucker, B. A. (In Press). CRISPR-Cas9 genome engineering: Treating inherited retinal degeneration. Progress in retinal and eye research. PMID: 29578069.

Giacalone, J. C., Sharma, T. P., Burnight, E. R., Fingert, J. F., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2018). CRISPR-Cas9-Based Genome Editing of Human Induced Pluripotent Stem Cells. Current protocols in stem cell biology, 44, 5B.7.1-5B.7.22. PMID: 29512106.

Burnight, E. R., Bohrer, L. R., Giacalone, J. C., Klaahsen, D. L., Daggett, H. T., East, J. S., Madumba, R. A., Worthington, K. S., Mullins, R. F., Stone, E. M., Tucker, B. A. & Wiley, L. A. (2018). CRISPR-Cas9-Mediated Correction of the 1.02?kb Common Deletion in CLN3 in Induced Pluripotent Stem Cells from Patients with Batten Disease. The CRISPR journal, 1, 75-87. PMID: 31021193.

Chirco, K. R., Lewis, C. J., Scheetz, T. E., Johnston, R. M., Tucker, B. A., Stone, E. M., Fingert, J. H. & Mullins, R. F. (2018). Evaluation of sFLT1 protein levels in human eyes with the FLT1 rs9943922 polymorphism. Ophthalmic genetics, 39(1), 68-72. PMID: 28949775.

Han, I. C., Jiao, C., Kaalberg, E. E., Bhattarai, S., Burnight, E. R., Tucker, B. A., Mullins, R. F. & Stone, E. M. (2018). Assessment of transduction efficiency and tropism of different adeno-associated virus (AAV) serotypes based on route of delivery in rats. (Vols. 59). (9) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.

Burnight, E. R., Gupta, M., Wiley, L. A., Anfinson, K. R., Tran, A., Triboulet, R., Hoffmann, J. M., Klaahsen, D. L., Andorf, J. L., Jiao, C., Sohn, E. H., Adur, M. K., Ross, J. W., Mullins, R. F., Daley, G. Q., Schlaeger, T. M., Stone, E. M. & Tucker, B. A. (2017). Using CRISPR-Cas9 to Generate Gene-Corrected Autologous iPSCs for the Treatment of Inherited Retinal Degeneration. Molecular therapy, 25(9), 1999-2013. PMID: 28619647.

Stone, E. M., Andorf, J. L., Whitmore, S. S., DeLuca, A. P., Giacalone, J. C., Streb, L. M., Braun, T. A., Mullins, R. F., Scheetz, T. E., Sheffield, V. C. & Tucker, B. A. (2017). Clinically Focused Molecular Investigation of 1000 Consecutive Families with Inherited Retinal Disease. Ophthalmology, 124(9), 1314-1331. PMID: 28559085.

Wiley, L. A., Anfinson, K. R., Cranston, C. M., Kaalberg, E. E., Collins, M. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2017). Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy. Current protocols in stem cell biology, 42, 4A.12.1 - 4A.12.14. PMID: 28806854.

Chirco, K. R., Worthington, K. S., Flamme-Wiese, M. J., Riker, M. J., Andrade, J. D., Ueberheide, B. M., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2017). Preparation and evaluation of human choroid extracellular matrix scaffolds for the study of cell replacement strategies. Acta biomaterialia, 57, 293-303. PMID: 28483697.

Songstad, A. E., Worthington, K. S., Chirco, K. R., Giacalone, J. C., Whitmore, S. S., Anfinson, K. R., Ochoa, D., Cranston, C. M., Riker, M. J., Neiman, M., Stone, E. M., Mullins, R. F. & Tucker, B. A. (2017). Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell-Derived Choroidal Endothelium. Stem cells translational medicine, 6(6), 1533-1546. PMID: 28474838.

Worthington, K. S., Wiley, L. A., Kaalberg, E. E., Collins, M. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2017). Two-photon polymerization for production of human iPSC-derived retinal cell grafts. Acta biomaterialia, 55, 385-395. PMID: 28351682.

Zhu, W., Jain, A., Gramlich, O. W., Tucker, B. A., Sheffield, V. C. & Kuehn, M. H. (2017). Restoration of Aqueous Humor Outflow Following Transplantation of iPSC-Derived Trabecular Meshwork Cells in a Transgenic Mouse Model of Glaucoma. Investigative ophthalmology & visual science, 58(4), 2054-2062. PMID: 28384726.

Sharma, T. P., Wiley, L. A., Whitmore, S. S., Anfinson, K. R., Cranston, C. M., Oppedal, D. J., Daggett, H. T., Mullins, R. F., Tucker, B. A. & Stone, E. M. (2017). Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa. Stem cell research, 21, 58-70. PMID: 28390992.

Chirco, K. R., Sohn, E. H., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2017). Structural and molecular changes in the aging choroid: implications for age-related macular degeneration. Eye (London, England), 31(1), 10-25. PMID: 27716746.

Worthington, K. S., Wiley, L. A., Mullins, R. F., Tucker, B. A. & Nuxoll, E. (2016). Prevascularized silicon membranes for the enhancement of transport to implanted medical devices. Journal of biomedical materials research. Part B, Applied biomaterials, 104(8), 1602-1609. PMID: 26316050.

Chirco, K. R., Whitmore, S. S., Wang, K., Potempa, L. A., Halder, J. A., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2016). Monomeric C-reactive protein and inflammation in age-related macular degeneration. The Journal of pathology, 240(2), 173-83. PMID: 27376713.

Wiley, L. A., Burnight, E. R., DeLuca, A. P., Anfinson, K. R., Cranston, C. M., Kaalberg, E. E., Penticoff, J. A., Affatigato, L. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness. Scientific reports, 6, 30742. PMID: 27471043.

Wiley, L. A., Burnight, E. R., Drack, A. V., Banach, B. B., Ochoa, D., Cranston, C. M., Madumba, R. A., East, J. S., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). Using Patient-Specific Induced Pluripotent Stem Cells and Wild-Type Mice to Develop a Gene Augmentation-Based Strategy to Treat CLN3-Associated Retinal Degeneration. Human gene therapy. PMID: 27400765.

Zhu, W., Gramlich, O. W., Laboissonniere, L., Jain, A., Sheffield, V. C., Trimarchi, J. M., Tucker, B. A. & Kuehn, M. H. (2016). Transplantation of iPSC-derived TM cells rescues glaucoma phenotypes in vivo. Proceedings of the National Academy of Sciences of the United States of America, 113(25), E3492-500. PMID: 27274060.

Worthington, K. S., Wiley, L. A., Guymon, C. A., Salem, A. K. & Tucker, B. A. (2016). Differentiation of Induced Pluripotent Stem Cells to Neural Retinal Precursor Cells on Porous Poly-Lactic-co-Glycolic Acid Scaffolds. Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 32(5), 310-6. PMID: 26692377.

Tucker, B. A. (2016). Using Stem Cells to Rebuild the Outer Neural Retina. Investigative ophthalmology & visual science, 57(7), 3521. PMID: 27367587.

Wiley, L. A., Beebe, D. C., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). A Method for Sectioning and Immunohistochemical Analysis of Stem Cell-Derived 3-D Organoids. Current protocols in stem cell biology, 37, 1C.19.1-1C.19.11. PMID: 27171793.

Worthington, K. S., Green, B. J., Rethwisch, M., Wiley, L. A., Tucker, B. A., Guymon, C. A. & Salem, A. K. (2016). Neuronal Differentiation of Induced Pluripotent Stem Cells on Surfactant Templated Chitosan Hydrogels. Biomacromolecules, 17(5), 1684-95. PMID: 27008004.

Chirco, K. R., Tucker, B. A., Stone, E. M. & Mullins, R. F. (2016). Selective accumulation of the complement membrane attack complex in aging choriocapillaris. Experimental eye research, 146, 393-7. PMID: 26368849.

Giacalone, J. C., Wiley, L. A., Burnight, E. R., Songstad, A. E., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). Concise Review: Patient-Specific Stem Cells to Interrogate Inherited Eye Disease. Stem cells translational medicine, 5(2), 132-40. PMID: 26683869.

Zeng, S., Whitmore, S. S., Sohn, E. H., Riker, M. J., Wiley, L. A., Scheetz, T. E., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2016). Molecular response of chorioretinal endothelial cells to complement injury: implications for macular degeneration. The Journal of pathology, 238(3), 446-56. PMID: 26564985.

DeLuca, A. P., Whitmore, S. S., Barnes, J., Sharma, T. P., Westfall, T. A., Scott, C. A., Weed, M. C., Wiley, J. S., Wiley, L. A., Johnston, R. M., Schnieders, M. J., Lentz, S. R., Tucker, B. A., Mullins, R. F., Scheetz, T. E., Stone, E. M. & Slusarski, D. C. (2016). Hypomorphic mutations in TRNT1 cause retinitis pigmentosa with erythrocytic microcytosis. Human molecular genetics, 25(1), 44-56. PMID: 26494905.

Small, K. W., DeLuca, A. P., Whitmore, S. S., Rosenberg, T., Silva-Garcia, R., Udar, N., Puech, B., Garcia, C. A., Rice, T. A., Fishman, G. A., Héon, E., Folk, J. C., Streb, L. M., Haas, C. M., Wiley, L. A., Scheetz, T. E., Fingert, J. H., Mullins, R. F., Tucker, B. A. & Stone, E. M. (2016). North Carolina Macular Dystrophy Is Caused by Dysregulation of the Retinal Transcription Factor PRDM13. Ophthalmology, 123(1), 9-18. PMID: 26507665.

Songstad, A. E., Wiley, L. A., Duong, K., Kaalberg, E., Flamme-Wiese, M. J., Cranston, C. M., Riker, M. J., Levasseur, D., Stone, E. M., Mullins, R. F. & Tucker, B. A. (2015). Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration. Investigative ophthalmology & visual science, 56(13), 8258-67. PMID: 26720480.

Tucker, B. A., Cranston, C. M., Anfinson, K. A., Shrestha, S., Streb, L. M., Leon, A., Mullins, R. F. & Stone, E. M. (2015). Using patient-specific induced pluripotent stem cells to interrogate the pathogenicity of a novel retinal pigment epithelium-specific 65 kDa cryptic splice site mutation and confirm eligibility for enrollment into a clinical gene augmentation trial. Translational research : the journal of laboratory and clinical medicine, 166(6), 740-749.e1. PMID: 26364624.

Sohn, E. H., Jiao, C., Kaalberg, E., Cranston, C., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2015). Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Scientific reports, 5, 11791. PMID: 26138532.

Drivas, T. G., Wojno, A. P., Tucker, B. A., Stone, E. M. & Bennett, J. (2015). Basal exon skipping and genetic pleiotropy: A predictive model of disease pathogenesis. Science translational medicine, 7(291), 291ra97. PMID: 26062849.

Tucker, B. A., Park, I. H., Qi, S. D., Klassen, H. J., Jiang, C., Yao, J., Redenti, S., Daley, G. Q. & Young, M. J. (2015). Correction: Transplantation of Adult Mouse iPS Cell-Derived Photoreceptor Precursors Restores Retinal Structure and Function in Degenerative Mice. (Vols. 10). (5), pp. e0125947. PloS one. PMID: 25950705.

Yao, J., Ko, C. W., Baranov, P. Y., Regatieri, C. V., Redenti, S., Tucker, B. A., Mighty, J., Tao, S. L. & Young, M. J. (2015). Enhanced differentiation and delivery of mouse retinal progenitor cells using a micropatterned biodegradable thin-film polycaprolactone scaffold. Tissue engineering. Part A, 21(7-8), 1247-60. PMID: 25517296.

Whitmore, S. S., Sohn, E. H., Chirco, K. R., Drack, A. V., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2015). Complement activation and choriocapillaris loss in early AMD: implications for pathophysiology and therapy. Progress in retinal and eye research, 45, 1-29. PMID: 25486088.

Wiley, L. A., Burnight, E. R., Songstad, A. E., Drack, A. V., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2015). Patient-specific induced pluripotent stem cells (iPSCs) for the study and treatment of retinal degenerative diseases. Progress in retinal and eye research, 44, 15-35. PMID: 25448922.

Gamm, D. M., Wong, R. (2015). Report on the National Eye Institute Audacious Goals Initiative: Photoreceptor Regeneration and Integration Workshop. TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, 4(6). DOI: 10.1167/tvst.4.6.2.

Wiley, L. A., Burnight, E. R., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2014). Stem Cells as Tools for Studying the Genetics of Inherited Retinal Degenerations. Cold Spring Harbor perspectives in medicine. PMID: 25502747.

Whitmore, S. S., Wagner, A. H., DeLuca, A. P., Drack, A. V., Stone, E. M., Tucker, B. A., Zeng, S., Braun, T. A., Mullins, R. F. & Scheetz, T. E. (2014). Transcriptomic analysis across nasal, temporal, and macular regions of human neural retina and RPE/choroid by RNA-Seq. Experimental eye research, 129, 93-106. PMID: 25446321.

Burnight, E. R., Wiley, L. A., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2014). Gene Therapy Using Stem Cells. [review]. Cold Spring Harbor perspectives in medicine, 5(4). PMID: 25395375.

Mullins, R. F., Schoo, D. P., Sohn, E. H., Flamme-Wiese, M. J., Workamelahu, G., Johnston, R. M., Wang, K., Tucker, B. A. & Stone, E. M. (2014). The membrane attack complex in aging human choriocapillaris: relationship to macular degeneration and choroidal thinning. The American journal of pathology, 184(11), 3142-53. PMID: 25204844.

Ding, Q. J., Zhu, W., Cook, A. C., Anfinson, K. R., Tucker, B. A. & Kuehn, M. H. (2014). Induction of trabecular meshwork cells from induced pluripotent stem cells. Investigative ophthalmology & visual science, 55(11), 7065-72. PMID: 25298418.

Tucker, B. A., Mullins, R. F. & Stone, E. M. (2014). Stem cells for investigation and treatment of inherited retinal disease. Human molecular genetics, 23(R1), R9-R16. PMID: 24647603.

Ksander, B. R., Kolovou, P. E., Wilson, B. J., Saab, K. R., Guo, Q., Ma, J., McGuire, S. P., Gregory, M. S., Vincent, W. J., Perez, V. L., Cruz-Guilloty, F., Kao, W. W., Call, M. K., Tucker, B. A., Zhan, Q., Murphy, G. F., Lathrop, K. L., Alt, C., Mortensen, L. J., Lin, C. P., Zieske, J. D., Frank, M. H. & Frank, N. Y. (2014). ABCB5 is a limbal stem cell gene required for corneal development and repair. Nature, 511(7509), 353-7. PMID: 25030174.

Tucker, B. A., Solivan-Timpe, F., Roos, B. R., Anfinson, K. R., Robin, A. L., Wiley, L. A., Mullins, R. F. & Fingert, J. H. (2014). Duplication of TBK1 Stimulates Autophagy in iPSC-derived Retinal Cells from a Patient with Normal Tension Glaucoma. Journal of stem cell research & therapy, 3(5), 161. PMID: 24883232.

Burnight, E. R., Wiley, L. A., Drack, A. V., Braun, T. A., Anfinson, K. R., Kaalberg, E. E., Halder, J. A., Affatigato, L. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2014). CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype. Gene Ther, 21(7), 662-72. PMID: 24807808.

Mullins, R. F., Khanna, A., Schoo, D. P., Tucker, B. A., Sohn, E. H., Drack, A. V. & Stone, E. M. (2014). Is age-related macular degeneration a microvascular disease?. Adv Med Biol, 801, 283-9. PMID: 24664709.

Sohn, E. H., Flamme-Wiese, M. J., Whitmore, S. S., Wang, K., Tucker, B. A. & Mullins, R. F. (2014). Loss of CD34 Expression in Aging Human Choriocapillaris Endothelial Cells. PLoS One, 9(1), e86538. PMID: 24466138.

Baranov, P. Y., Tucker, B. A. & Young, M. J. (2014). Low-oxygen culture conditions extend the multipotent properties of human retinal progenitor cells. Tissue engineering Part A, 20(9-10), 1465-75. PMID: 24320879.

Worthington, K. S., Wiley, L. A., Bartlett, A. M., Stone, E. M., Mullins, R. F., Salem, A. K., Guymon, C. A. & Tucker, B. A. (2014). Mechanical properties of murine and porcine ocular tissues in compression. Exp Eye Res, 121, 194-9. PMID: 24613781.

Boye, S. E., Huang, W. C., Roman, A. J., Sumaroka, A., Boye, S. L., Ryals, R. C., Olivares, M. B., Ruan, Q., Tucker, B. A., Stone, E. M., Swaroop, A., Cideciyan, A. V., Hauswirth, W. W. & Jacobson, S. G. (2014). Natural History of Cone Disease in the Murine Model of Leber Congenital Amaurosis Due to CEP290 Mutation: Determining the Timing and Expectation of Therapy. PLoS One, 9(3), e92928. PMID: 24671090.

Thompson, S., Blodi, F. R., Lee, S., Welder, C. R., Mullins, R. F., Tucker, B. A., Stasheff, S. F. & Stone, E. M. (2014). Photoreceptor cells with profound structural deficits can support useful vision in mice. Invest Ophthalmol Vis Sci, 55(3), 1859-66. PMID: 24569582.

Sohn, E. H., Khanna, A., Tucker, B. A., Abramoff, M. D., Stone, E. M. & Mullins, R. F. (2014). Structural and biochemical analyses of choroidal thickness in human donor eyes. Invest Ophthalmol Vis Sci, 55(3), 1352-60. PMID: 24519422.

Braun, T. A., Mullins, R. F., Wagner, A. H., Andorf, J. L., Johnston, R. M., Bakall, B. B., Deluca, A. P., Fishman, G. A., Lam, B. L., Weleber, R. G., Cideciyan, A. V., Jacobson, S. G., Sheffield, V. C., Tucker, B. A. & Stone, E. M. (2013). Non-exomic and synonymous variants in ABCA4 are an importnant cause of Stargardt disease. Hum Mol Genet, 22(25), 5136-45. PMID: 23918662.

Tucker, B. A., Mullins, R. F., Streb, L. M., Anfinson, K., Eyestone, M. E., Kaalberg, E., Riker, M. J., Drack, A. V., Braun, T. A. & Stone, E. M. (2013). Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa. eLife, 2, e00824. PMID: 23991284.

Tucker, B. A., Anfinson, K. R., Mullins, R. F., Stone, E. M. & Young, M. J. (2013). Use of a Synthetic Xeno-Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation. Stem Cells Transl Med, 2(1), 16-24. PMID: 23283489.

Checa-Casalengua, P., Jiang, C., Bravo-Osuna, I., Tucker, B. A., Young, M. J. & Herrero-Vanrell, R. (2012). Preservation of Biological Activity of Glial Cell Line Derived Neurotrophic Factor (GDNF) After Microencapsulation adn Sterilization by Gamma Irradiation. Int J Pharm, 436(1-2), 545-54. PMID: 22828071.

Velez, G., Weingarden, A. R., Tucker, B. A., Lei, H., Kazlauskas, A. & Young, M. J. (2012). Retinal Pigment Epithelium and Muller Progenitor Cell Interaction Increase Muller Progenitor Cell Expression of PDGFRa and Ability to Induce Proliferative Vitreoretinopathy in a Rabbit Model. Stem Cell Int, 2012, 106486. PMID: 22966235.

Klassen, H., Tucker, B. A., Liew, C. G., La Cour, M. & Wang, H. C. (2012). Stem Cells in Large Animal Models of Retinal and Neurological Disease. Stem Cells Int, 2012, 460504. PMID: 22666270.

Tucker, B. A., Sheetz, T. E., Mullins, R. F., Deluca, A. P., Hoffmann, J. M., Johnston, R. M., Jacobson, S. G., Sheffield, V. C. & Stone, E. M. (2011). Exome Sequencing and Analysis of Induced Pluripotent Stem Cells Identify the Cilia-Related Gene Male Germ Cell-Associated Kinase (MAK) as a Cause of Retinitis Pigmentosa. Proc Natl Acad Sci USA, 108(34), E569-76. PMID: 21825139.

Ma, J., Kabiel, M., Tucker, B. A., Ge, J. & Young, M. J. (2011). Combining Chondroitinase ABC and Growth Factors Promotes the Integration of Murine Retinal Progenitor Cells Transplanted into Rho (-/-) Mice. Molecular Vision,(17), 1759-70. PMID: 21750603.

Checa-Casalengua, P., Jiang, C., Bravo-Osuna, I., Tucker, B. A., Molina-Martinez, I. T., Young, M. J. & Herrero-Vanrell, R. (2011). Retinal Ganglion Cells Survival in Glaucoma Model by GDNF/Vit E PLGA Microsphere Prepared According to a Novel Microencapsulation Procedure. J Control Release, 156(1), 92-100. PMID: 21704662.

Yao, J., Tucker, B. A., Zhang, X., Checa-Casalengua, P., Herrero-Vanrell, R. & Young, M. J. (2011). Robust Cell Integration From Co-Transplantation of Biodegradable MMP2-PLGA Microspheres with Retinal Progenitor Cells. Biomaterials, 32(4), 1041-50. PMID: 21030072.

Tucker, B. A., Park, I. H., Klassen, H. J., Redenti, S., Jiang, C., Qi, S. D., Daley, G. Q. & Young, M. J. (2011). Transplantation of Adult Mouse iPS Cell-Derived Photoreceptor Precursors Restore Retinal Structure and Function in Retinal Degenerative Mice. (Vols. 6). (4), pp. e18992. Plos One. PMID: 21559507.

Ejstrup, R., Kiilgaard, J. F., Tucker, B. A., Klassen, H. J., Young, M. J. & La Cour, M. (2010). Pharmacokinetics of intravitreal glial cell line-derived neurotrophic factor: experimental studies in pigs. Experimental eye research, 91(6), 890-5. PMID: 20883692.

Tucker, B. A., Karamsadkar, S. S., Khan, W. S. & Pastides, P. (2010). The role of bone marrow derived mesenchymal stem cells in sports injuries. Journal of stem cells, 5(4), 155-66. PMID: 22314864.

Aftab, U., Jiang, C., Tucker, B. A., Kim, J. Y., Klassen, H. J., Miljan, E., Sinden, J. & Young, M. J. (2009). Growth Kinetics and Transplantation of Human Retinal Progenitor Cells. Experimental Eye Research, 89(3), 301-10.

Tackenberg, M. A., Tucker, B. A., Swift, J. S., Jiang, C., Redenti, S., Greenberg, K. P., Flannery, J. G., Reichenbach, A. & Young, M. J. (2009). Müller cell activation, proliferation and migration following laser injury. Molecular vision, 15, 1886-96. PMID: 19768129.

Tucker, B. A., Young, M. J. & Klassen, H. J. (2009). Stem Cells and Retinal Regeneration. In Encyclopedia of the Eye. Dartt, D., Dana, R., & Bok, D..

Tucker, B. A., Redenti, S., Jiang, C., Swift, J. S., Klassen, H. J., Smith, M. E., Wnek, G. E. & Young, M. J. (2009). The Use of Progenitor Cell/Biodegradable MMP2-PLGA Polymer Constructs to Enhance Cellular Integration and Retinal Repopulation. Biomaterials, 31(1), 9-19. PMID: 19775744.

Tucker, B. A., Mearow, K. M. (2008). Peripheral sensory axon growth: from receptor binding to cellular signaling. The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques, 35(5), 551-66. PMID: 19235438.

Tucker, B. A., Klassen, H., Yang, Y., Chen, D. F. & Young, M. J. (2008). Elevated MMP Expression in the MRL Mouse Retina Creates a Permissive Environment for Retinal Regeneration. Investigative Ophthalmoology & Visual Science,(49), 1686-1695. PMID: 18385092.

Saint-Geniez, M., Maharaj, A. S., Walshe, T. E., Tucker, B. A., Sekiyama, E., Kurihara, T., Darland, D. C., Young, M. J. & D'Amore, P. A. (2008). Endogenous VEGF is required for visual function: evidence for a survival role on mÕ_ller cells and photoreceptors. PloS one, 3(11), e3554. PMID: 18978936.

Tucker, B. A., Klassen, H. J. & Young, M. J. (2008). Repopulation of the Retina.

Tucker, B. A., Rahimtula, M. & Mearow, K. M. (2008). Src and Fak are Key Early Signaling Intermediates Required for Neurite Growth in NGF-Responsive Adult DRG Neurons. Cellular Signaling, 20(1), 241-257. PMID: 17999938.

Ploughman, M., Granter-Button, S., Chernenko, G., Attwood, Z., Tucker, B. A., Mearow, K. M. & Corbett, D. (2007). Exercise intensity influences the temporal profile of growth factors involved in neuronal plasticity following focal ischemia. Brain research, 1150, 207-16. PMID: 17382914.

Young, M. J., Tucker, B. A. & Klassen, H. J. (2007). Stemming the Tide of Retinal Degeneration. Retinal Physician.

Zhang, Y., Klassen, H. J., Tucker, B. A., Perez, M. T. & Young, M. J. (2007). CNS Progenitor Cells Promote a Permissive Environment for Neurite Outgrowth via a Matrix Metalloproteinase-2-Dependent Mechanism. Journal of Neuroscience, 27(17), 4499-506.

ploughman, M., Granter-Button, S., Chernenko, G., Attwood, Z., Tucker, B. A., Mearow, K. M. & Corbett, D. (2007). Exercise intensity influences the temporal profile of growth factors involved in neuronal plasticity following focal ischemia. Brain Research,(1150), 207-16. PMID: 17382914.

Young, M. J., Tucker, B. A. & Klassen, H. J. (2007). Stemming the tide of retinal degeneration.

Tucker, B. A., Rahimtula, M. & Mearow, K. M. (2006). Laminin and growth factor receptor activation stimulates differential growth responses in subpopulations of adult DRG neurons. The European journal of neuroscience, 24(3), 676-90. PMID: 16930399.

Tucker, B. A. (2006). Cooperation Between Integrin and Growth Factor Receptor Signalling in the Regulation of Neurite Growth From Rat Sensory Neurons.

Tucker, B. A., Rahimtula, M. & Mearow, K. M. (2006). Laminin and growth factor receptor activation stimulates differential growth responses in subpopulations of adult DRG neurons: Differential growth responses in subpopulations of DRG neurons. European Journal of Neuroscience, 24(3), 676-90.

Tucker, B. A., Rahimtula, M. & Mearow, K. M. (2005). A procedure for selecting and culturing subpopulations of neurons from rat dorsal root ganglia using magnetic beads. Brain Research. Brain Research Protocols, 16(42738), 50-57. PMID: 16309950.

Ploughman, M., Granter-Button, S., Chernenko, G., Tucker, B. A., Mearow, K. M. & Corbett, D. (2005). Endurance Exercise Regimens Induce Differential Effects on BDNF, Synapsin-I and IGF-I After Focal Ischemia. Neuroscience, 136(4), 991-1001.

Tucker, B. A., Rahimtula, M. & Mearow, K. M. (2005). Integrin Activation and neurotrophin Signaling Cooperate to Enhance Neurite Outgrowth in Sensor Neurons. Journal of Comparative Neurology, 486(3), 267-280. PMID: 15844170.

Jones, D. M., Tucker, B. A., Rahimtula, M. & Mearow, K. M. (2003). The Synergistic Effects of NGF and IGF-1 on Neurite Growth in Adult Sensory neurons: Convergence on the PI 3-Kinase Signaling Pathway. Journal of Neurochemistry, 86(3), 1116-1128.

Tucker, B. A. (2001). Textual Recall in Undergraduate University Students.

Tucker, B. A., Legge, R., Walbourne, S. & Wilson, B. P. (1999). Western Health Care Corporation Patient, Staff, and Physician Satisfaction.

Tucker, B. A., Legge, R., Walbourne, S. & Wilson, B. P. (1998). Western Health Care Corporation Patient, Staff, and Physician Satisfaction.