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Analysis vs. Cell Sorting—What’s the Difference?

Analyzing – Cytek Aurora and Becton Dickinson LSR II

Analyzing involves using a flow cytometer to interrogate cells stained with fluorescent markers and classifying them into groups. Cells pass through the instrument once and go straight to a waste container. There is no cell recovery.

Cell Sorting – Becton Dickinson FACS Aria II, FACS Fusion

Cell sorting involves using a flow cytometer with cell sorting capability to interrogate cells stained with fluorescent markers, classifying them into groups, and then physically separate the groups into test tubes or multi-well plates. The fraction of the starting population defined by the gating strategy is recovered, the remainder of cells go to a waste container.