0.02mg/ml DNase I (type IIS) to help eliminate clumping (Sigma-Aldrich Cat. No. D4513-VL).

Add 0.02mg/ml DNase I (type IIS) to all cell preparation steps, including wash steps, to eliminate free DNA from broken cells that leads to aggregation. Cations must be available to the DNase in order to work properly, i.e., avoid using EDTA.

Accumax, commercial product for keeping cells dissociated.

Pluronic F-68 to help eliminate clumping.

The following suggestion is from Steven Z. Merlin, Columbia University.

"For cell anti-aggregation reagents, try Pluronic F-68 non-toxic, non-ionic surfactant. It is commonly used in bio-fermenters and I have found it to keep various concentrated samples of primary cells and cell lines from forming aggregates during very long sorts. Also works very well for hepatocytes, IEL's, CHO, fibroblasts, and many other cell types having a propensity to form aggregates. It also works well for analyzing and sorting mixed phytoplankton strains from sea water."

" Pluronic is sold by ThermoFisher (catalog number 24040032) as a 10% stock solution in a 100mL bottle. Add to your sample to make a final concentration of 1% vol/vol."