Cytek Aurora with 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm lasers

Producing tSNE plots in FlowJo v10

Setting FlowJo 10 Scale Values for Cytek Aurora Files

Instrument Details

Five Excitation Sources

20 mW at 355 nm (UV)
100 mW at 405 nm (violet)
50 mW at 488 nm (blue)
50 mW at 561 nm (yellow-green)
80 mW at 640 nm (red)

64 Fluorescence Emission Detection Channels (providing measurement of the full visible spectrum for each fluorochrome)

Ultra-violet detector module: 16 channels
Violet detector module: 16 channels
Blue detector module: 14 channels
Yellow-Green detector module: 10 channels
Red detector module: 8 channels

Automatic Micro-Sampling System (AMS)

Sample cells from a U-bottom, V-bottom, or flat bottom standard-height 96-well plate

Pulse Processing

Pulse area and height measurement available for all channels
Pulse width of forward and side scatter
Pulse width of one fluorescence parameter for each laser

Microparticle Detection

Violet side scatter detector for thresholding on microparticles

Volumetric Measurement

Direct volumetric measurement for cell counting