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Five-laser Cytek Aurora User Guide
UI Training Slides
- Five Laser Cytek Aurora Fluorochrome Selection
- Cytek fluorescence spectral viewer (specific to Cytek spectral analyzers)
- Youtube Software and Hardware Tutorial Videos
Aurora Quick Reference Guide
- Producing tSNE plots in FlowJo v10
- Setting FlowJo 10 Scale Values for Cytek Aurora Files
Instrument Details
Five Excitation Sources
- 20 mW at 355 nm (UV)
- 100 mW at 405 nm (violet)
- 50 mW at 488 nm (blue)
- 50 mW at 561 nm (yellow-green)
- 80 mW at 640 nm (red)
64 Fluorescence Emission Detection Channels (providing measurement of the full visible spectrum for each fluorochrome)
- Ultra-violet detector module: 16 channels
- Violet detector module: 16 channels
- Blue detector module: 14 channels
- Yellow-Green detector module: 10 channels
- Red detector module: 8 channels
Automatic Micro-Sampling System (AMS)
- Sample cells from a U-bottom, V-bottom, or flat bottom standard-height 96-well plate
Pulse Processing
- Pulse area and height measurement available for all channels
- Pulse width of forward and side scatter
- Pulse width of one fluorescence parameter for each laser
Microparticle Detection
- Violet side scatter detector for thresholding on microparticles
Volumetric Measurement
- Direct volumetric measurement for cell counting