Designing a multicolor immunofluorescent antibody panel that optmizes fluorochromes with antigen density can be challenging given the abundance of antibody vendors, fluorochrome choices, and instrument configurations. The links on this page take you to tools that help streamline this process. Each of the panel design tools is tied to multiple antibody-vendor databases, making it simpler to find the right combination of antibodies and fluorochromes that match the instrument used for your experiment. Once the panel is designed, each allows you to save and print the panel for purchasing the antibodies.
Facility staff are happy to help in the design of your panel and encourage you to let us review your panel before ordering antibodies. By reviewing your antibody panel, we may be able to help you choose the best fluorochromes for a given instrument configuration. Switching one or two colors can have dramatic affects on the separation of positive from negative cell populations.
- The information at the following links show the facility's current multicolor instrument configurations. This information is necessary in order to successfully use some of the panel designer tools.
FACS Aria II (cell sorter) laser, optical filter, and fluorochrome configurations- FACS Fusion (cell sorter) laser, optical filter, and fluorochrome configurations
- Violet LSR (analyzer) laser, optical filter, and fluorochrome configurations
- UV LSR (analyzer) laser, optical filter, and fluorochrome configurations
- Accuri (analyzer) laser, optical filter and fluorochrome configurations
- Includes all of the instruments in the main lab of the UI Flow Cytometry Facility.
- Requires an account to be established before saving instrument and panel configurations.
- Video showing Chromocyte's panel designer
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Includes all of the instruments in the main lab of the UI Flow Cytometry Facility.