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Statistics
Calculation for Comparing Populations (Staining Index)
This calculation can be used for normalizing the relationship between positive and negative populations to compare treated and untreated samples.
SI = (MFIpos – MFIneg ) / (2 x SDneg)
SI = staining index
MFI = median, geometric-mean, or mean fluorescence intensity
SD = standard deviation.
Note: Population average fluorescence intensity values can be skewed depending on the population distribution. Median fluorescence intensity is less skewed than geometric-mean fluorescence intensity and geometric-mean fluorescence intensity is less skewed than mean fluorescence intensity for log-normal populations (populations that look gaussian when plotted on a log scale).
FlowJo v10
FlowJo v10 seminar slides (March 1, 2017) – Graciously provided by Dr. Tim Crawford
- Web based FlowJo tutorials
- Producing tSNE plots in FlowJo v10
- Setting FlowJo 10 Scale Values for Cytek Aurora Files
Fluorescence Spectral Viewers
- Becton Dickinson
- Thermo Fisher / eBiosciences / Invitrogen / Molecular Probes
- BioLegend
- Evrogen (for fluorescent proteins)
- Clontech (for fluorescent proteins)
- Chroma
- Semrock
Fluorochrome Brightness Comparisons
Antibody/Fluorochrome Conjugation Kits
Antibody Search Engines for Difficult to Find Antibodies
Dye and Probe References
Mobile Apps for Flow Cytometry and the Lab
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