Logo for University of Iowa Health Care This logo represents the University of Iowa Health Care

Tools and Links

University homepage and directory   University of Iowa homepage and directory

HawkID Guide   HawkID Guide

Purdue   Flow Cytometry Suppliers Directory

Purdue   Free Flow Cytometry Software

Purdue   Flow Cytometry Email Bulletin Board.


Calculation for Comparing Populations (Staining Index)

This calculation can be used for normalizing the relationship between positive and negative populations to compare treated and untreated samples.

SI = (MFIpos – MFIneg ) / (2 x SDneg

SI = staining index
MFI = median, geometric-mean, or mean fluorescence intensity
SD = standard deviation.

Note: Population average fluorescence intensity values can be skewed depending on the population distribution. Median fluorescence intensity is less skewed than geometric-mean fluorescence intensity and geometric-mean fluorescence intensity is less skewed than mean fluorescence intensity for log-normal populations (populations that look gaussian when plotted on a log scale).

FlowJo v10

Fluorescence Spectral Viewers

Fluorochrome Brightness Comparisons

Antibody/Fluorochrome Conjugation Kits

Antibody Search Engines for Difficult to Find Antibodies

Dye and Probe References

Mobile Apps for Flow Cytometry and the Lab

Information regarding commercial services and external links on this page are offered as a convenience. The University of Iowa does not endorse the services or products mentioned nor is it in any way responsible for the reliability of the services or products offered.