Shuttle plasmids are provided free of charge when intended for virus production at the University of Iowa Viral Vector Core. (A $25 FedEx overnight shipping fee will apply if no FedEx account is provided). Shuttles with the pFBAAV backbone can be used for either the triple transfection or baculovirus system. Several of the self-complementary plasmids are only for use in the triple transfection system.
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Non-Self Complementary Shuttle Plasmids
ITR to ITR cassette size is limited to ~4.7kb
G0202 pFBAAVmcsBgHpA
Cat: G0202
pFBAAV backbone, multiple cloning site, Bovine Growth Hormone polyadenylation signal
G0347 pFBAAVCMVmcspBgHpA
Cat: G0347
pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal
G0261 pFBAAVRSVmcsBgHpA
Cat: G0261
pFBAAV backbone, RSV (rocus sarcoma virus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal
G0345 pFBAAVCAGmcsBgHpA
Cat: G0345
pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, bovine growth hormone polyadenylation signal
G0746 pFBAAVmcswtIRESeGFPBgHpA
Cat: G0746
pFBAAV backbone, multiple cloning site, wild type IRES (internal ribosome entry site), eGFP (enhanced green flourescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.
G0692 pFBAAVCMVmcswtIRESeGFPBgHpA
Cat: G0692
pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.
G0747 pFBAAVCAGmcswtIRESeGFPBgHpa
Cat: G0747
pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.
G1066 pFBAAVmcswtIRESmCherryBgHpA
Cat: G1066
pFBAAV backbone, multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.
G1068 pFBAAVCMVmcswtIRES
mCherryBgHpA
Cat: G1068
pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry, bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.
G1067 pFBAAVCAGmcswtIRES
mCherryBgHpA
Cat: G1067
pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry, bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.
G0358 pFBAAVmcsCMVhrGFPSV40pA
Cat: G0358
pFBAAV backbone, multiple cloning site, CMV (cytomegalovirus) promoter driving hrGFP (Humanized Renilla reniformis green fluorescent protein), SV40 polyadenylation signal.
G0619 pFBAAVmcsCMVeGFPSV40A
Cat: G0619
Extended mcs. pFBAAV backbone, multiple cloning site (more available sites), CMV (cytomegalovirus) promoter driving eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal
G0760 pFBAAVmU6mcsCMVeGFPSV40pA
Cat: G0760
pFBAAV backbone, mouse U6 promoter (RNA polymerase III), multiple cloning site designed for RNAi insertion, poly T termination sequence, CMV (cytomegalovirus) promoter driving eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal
G0774 pFBAAVmU6mcsCMVmCherrySV40pA
Cat: G0774
pFBAAV backbone, mouse U6 promoter (RNA polymerase III), multiple cloning site designed for RNAi insertion, poly T termination sequence, CMV (cytomegalovirus) promoter driving mCherry (red fluorescent protein), SV40 polyadenylation signal
G1006 pFBAAVmU6miSafeCMV
mCherrySV40pA
Cat: G1006
Scrambled control for testing use with cat: G0774.
Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.
G0784 pFBAAVmU6miSafeCMVeGFPSV40pA
Cat: G0784
Scrambled control for testing use with cat: G0760.
Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.
G1060 pFBAAVTREmcsBgHpACMVrtTA
wtIRESeGFPSV40pA
Cat: G1060
pFBAAV backbone, TRE (TET response element) promoter, multiple cloning site designed for RNAi insertion, bovine growth hormone polyadenylation signal, CMV (cytomegalovirus) promoter driving tetracycline-controlled transactivator (rtTA-M2), wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal. This plasmid was designed for inducible expression (TET-ON) of the miRNA cassette in the prescence of the antibiotic tetracycline (doxycycline).
G1061 pFBAAVTREmiSafeBgHpACMVrtTA
wtIRESeGFPSV40pA
Cat: G1061
Scrambled control for testing use with cat: G1060.
Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.
G0013 pFBGR
Cat: G0013
pFBAAV backbone, CMV (cytomegalovirus) promoter and P10 insect promoter driving eGFP (enhanced green fluorescent protein) , SV40pA polyadenylation signal.
Self-Complementary Shuttle Plasmids
ITR to ITR cassette size is limited to ~2.2kb.
G0463 pscAAVmcsBgHpa
Cat: G0463
Self-complementary plasmid for use with the triple transfection system only. Multiple cloning site, bovine growth hormone polyadenylation signal. The backbone is based on self-complementary AAV vectors kindly provided by Brian Kasper.
G0605 pscAAVmCMVmcsBgHpA
Cat: G0605
Self-complementary plasmid for use with the triple transfection system only. Modified CMV (cytomegalovirus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal. The backbone is based on self-complementary AAV vectors kindly provided by Brian Kasper.
G0978 pFBscAAVmcsBGHpA
Cat: 0978
pFBAAV backbone with self-complementary ITRs. Can be used with either the triple transfection system or baculovirus system. Multiple cloning site, bovine growth hormone polyadenylation signal.