Adeno-Associated Virus Shuttle Plasmids

Adeno-Associated VirusShuttle plasmids are provided free of charge when intended for virus production at the University of Iowa Viral Vector Core. (A $25 FedEx overnight shipping fee will apply if no FedEx account is provided). Shuttles with the pFBAAV backbone can be used for either the triple transfection or baculovirus system. Several of the self-complementary plasmids are only for use in the triple transfection system.  

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Please view our ordering information for shuttle plasmids to learn how to register for an account, place orders for in-stock vectors and make payment through our online system.

Non-Self Complementary Shuttle Plasmids

ITR to ITR cassette size is limited to ~4.7kb

G0202 pFBAAVmcsBgHpA

Cat: G0202

pFBAAV backbone, multiple cloning site, Bovine Growth Hormone polyadenylation signal


G0347 pFBAAVCMVmcspBgHpA

Cat: G0347

pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal


G0261 pFBAAVRSVmcsBgHpA

Cat: G0261

pFBAAV backbone, RSV (rocus sarcoma virus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal


G0345 pFBAAVCAGmcsBgHpA

Cat: G0345

pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, bovine growth hormone polyadenylation signal


G0746 pFBAAVmcswtIRESeGFPBgHpA

Cat: G0746

pFBAAV backbone, multiple cloning site, wild type IRES (internal ribosome entry site), eGFP (enhanced green flourescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.


G0692 pFBAAVCMVmcswtIRESeGFPBgHpA

Cat: G0692

pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.


G0747 pFBAAVCAGmcswtIRESeGFPBgHpa

Cat: G0747

pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.


G1066 pFBAAVmcswtIRESmCherryBgHpA

Cat: G1066

pFBAAV backbone, multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry (enhanced green fluorescent protein), bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.


G1068 pFBAAVCMVmcswtIRES
mCherryBgHpA

Cat: G1068

pFBAAV backbone, CMV (cytomegalovirus) promoter, multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry, bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.


G1067 pFBAAVCAGmcswtIRES
mCherryBgHpA

Cat: G1067

pFBAAV backbone, CAG promoter (CMV enhancer fused to the chicken beta-actin promoter), multiple cloning site, wild-type IRES (internal ribosome entry site), mCherry, bovine growth hormone polyadenylation signal. The IRES-mCherry functions as a non-fusion protein reporter without the addition of a second promoter.


G0358 pFBAAVmcsCMVhrGFPSV40pA

Cat: G0358

pFBAAV backbone, multiple cloning site, CMV (cytomegalovirus) promoter driving hrGFP (Humanized Renilla reniformis green fluorescent protein), SV40 polyadenylation signal.


G0619 pFBAAVmcsCMVeGFPSV40A

Cat: G0619

Extended mcs. pFBAAV backbone, multiple cloning site (more available sites), CMV (cytomegalovirus) promoter driving eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal


G0760 pFBAAVmU6mcsCMVeGFPSV40pA

Cat: G0760

pFBAAV backbone, mouse U6 promoter (RNA polymerase III), multiple cloning site designed for RNAi insertion, poly T termination sequence, CMV (cytomegalovirus) promoter driving eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal


G0774 pFBAAVmU6mcsCMVmCherrySV40pA

Cat: G0774

pFBAAV backbone, mouse U6 promoter (RNA polymerase III), multiple cloning site designed for RNAi insertion, poly T termination sequence, CMV (cytomegalovirus) promoter driving mCherry (red fluorescent protein), SV40 polyadenylation signal


G1006 pFBAAVmU6miSafeCMV
mCherrySV40pA

Cat: G1006

Scrambled control for testing use with cat: G0774.

Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.


G0784 pFBAAVmU6miSafeCMVeGFPSV40pA

Cat: G0784

Scrambled control for testing use with cat: G0760.

Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.


G1060 pFBAAVTREmcsBgHpACMVrtTA
wtIRESeGFPSV40pA

Cat: G1060

pFBAAV backbone, TRE (TET response element) promoter, multiple cloning site designed for RNAi insertion, bovine growth hormone polyadenylation signal, CMV (cytomegalovirus) promoter driving tetracycline-controlled transactivator (rtTA-M2), wild-type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein), SV40 polyadenylation signal. This plasmid was designed for inducible expression (TET-ON) of the miRNA cassette in the prescence of the antibiotic tetracycline (doxycycline).


G1061 pFBAAVTREmiSafeBgHpACMVrtTA
wtIRESeGFPSV40pA

Cat: G1061

Scrambled control for testing use with cat: G1060.

Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.


G0013 pFBGR

Cat: G0013

pFBAAV backbone, CMV (cytomegalovirus) promoter and P10 insect promoter driving eGFP (enhanced green fluorescent protein) , SV40pA polyadenylation signal.

 

 

Self-Complementary Shuttle Plasmids

ITR to ITR cassette size is limited to ~2.2kb.

G0463 pscAAVmcsBgHpa

Cat: G0463

Self-complementary plasmid for use with the triple transfection system only. Multiple cloning site, bovine growth hormone polyadenylation signal. The backbone is based on self-complementary AAV vectors kindly provided by Brian Kasper.


G0605 pscAAVmCMVmcsBgHpA

Cat: G0605

Self-complementary plasmid for use with the triple transfection system only. Modified CMV (cytomegalovirus) promoter, multiple cloning site, bovine growth hormone polyadenylation signal. The backbone is based on self-complementary AAV vectors kindly provided by Brian Kasper.


G0978 pFBscAAVmcsBGHpA

Cat: 0978

pFBAAV backbone with self-complementary ITRs. Can be used with either the triple transfection system or baculovirus system. Multiple cloning site, bovine growth hormone polyadenylation signal.