The University of Iowa Viral Vector Core provides shuttle plasmids at no cost when intended for virus production. (A $50 FedEx overnight shipping fee will apply if no FedEx account is provided). Material sent includes 10μl of plasmid. We suggest that you digest the plasmid after any amplification step.
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Please view our ordering information for shuttle plasmids to learn how to register for an account, place orders for shuttle plasmids and make payment through our online system.
Plasmid Backbone
The pFIV3.2 backbone is based on the pVETL Backbone developed by Novartis/Chiron Technologies and modified by Patrick L. Sinn, PhD (University of Iowa) to improve safety and transgene expression:
- The lentiviral central polypurine tract and the central termination sequence were added directly downstream of the gag sequence.
- The Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element, WPRE, was added directly upstream of the rev response element, RRE.
- The major splice donor and the initial gag sequence each have a mutation but the packaging signal was retained.
- The FIV 3’LTR was rendered “self-inactivating” by deleting a portion of the U3 region.
G0654 pFIV3.2mcs
Cat: G0654
pFIV3.2 backbone, multiple cloning site.
G0655 pFIV3.2CMVmcs
Cat: G0655
pFIV3.2 backbone, CMV (Cytomegalovirus) Promoter, multiple cloning site.
G0656 pFIV3.2RSVmcs
Cat: G0656
pFIV3.2 backbone, RSV (Rous sarcoma virus) Promoter, multiple cloning site.
G0667 pFIV3.2CAGmcs
Cat: G0667
pFIV3.2 backbone, CAG (CMV enhancer fused to the chicken beta-actin promoter) Promoter, multiple cloning site.
G0356 pFIV3.2TREmcs
Cat: G0356
pFIV3.2 backbone, TRE tight (TET Response Element) Promoter, multiple cloning site.
G0668 pFIV3.2EF1amcs
Cat: G0668
pFIV3.2 backbone, Human elongation factor-1 alpha (EF1a) Promoter, multiple cloning site..
G0863 pFIV3.2PGKmcs
Cat: G0863
pFIV3.2 backbone, PGK(Phosphoglycerate kinase) Promoter, multiple cloning site.
G0752 pFIV3.2mcswtIRESeGFP
Cat: G0752
pFIV3.2 backbone, multiple cloning site, wild type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein. The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.
G0754 pFIV3.2CMVmcswtIRESeGFP
Cat: G0754
pFIV3.2 backbone, multiple cloning site, wild type IRES (internal ribosome entry site), eGFP (enhanced green fluorescent protein). The IRES-eGFP functions as a non-fusion protein reporter without the addition of a second promoter.
G0411 pFIV3.2mcseGFP
Cat: G0411
pFIV3.2 backbone, multiple cloning site, eGFP (enhanced green fluorescent protein).
G0859 pFIV3.2CMVmcwtIRESZeocin
Cat: G0859
pFIV3.2 backbone, CMV (Cytomegalovirus) Promoter, multiple cloning site, wild type IRES (internal ribosome entry site), Zeocin as a selectable marker.
G0913 pFIV3.2CMVmcwtIRESNeomycin
Cat: G0913
pFIV3.2 backbone, CMV (Cytomegalovirus) Promoter, multiple cloning site, wild type IRES (internal ribosome entry site), Neomycin as a selectable marker.
G0776 pFIV3.2CAGmcswtIRESeGFP
Cat: G0776
pFIV3.2 backbone, CAG (CMV enhancer fused to the chicken beta-actin promoter) Promoter, multiple cloning site, wild type IRES (internal ribosome entry site), eGFP.
G0860 pFIV3.2mcswtIRESZeocin
Cat: G0860
pFIV3.2 backbone, multiple cloning site, wild type IRES (internal ribosome entry site), Zeocin as a selectable marker.
G0432 pFIV3.2mcsCMVeGFP
Cat: G0432
pFIV3.2 backbone backbone, multiple cloning site, CMV (Cytomegalovirus) Promoter, eGFP (enhanced green fluorescent protein). Suitable for RNAi cassettes.
G0973 pFIV3.2mU6shSafeCMVeGFP
Cat: G0973
pFIV3.2 backbone backbone, Mouse U6 Promoter driving a scrambled shRNA control shSafe cassette, CMV (Cytomegalovirus) Promoter, eGFP (enhanced green fluorescent protein).
Please reference: Boudreau, R. L., et al. (2011). "Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease." Mol Ther 19(12): 2169-2177.