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Gerene Denning, Ph.D.

Gerene DenningGerene Denning, Ph.D.

Adjunct Professor of Emergency Medicine

Contact Information

Office: 200 Hawkins Drive, 1008 RCP
Iowa City, IA 52242
Phone: 319-384-8123

Email: gerene-denning@uiowa.edu

Education

BA, Chemistry/Biochemistry,  Univ. of Iowa, Iowa City, IA

Biochemistry, Univ. Calif. at San Diego, La Jolla, CA

Ph.D., Biochemistry, University of Iowa, Iowa City, IA

Postdoctoral Associate,  Howard Hughes Medical Institute, Iowa City

Publications

  1. Spector, AA, Kiser, RE, Denning, GM, Koh, S-WM, and DeBault, LE.  Modification of the Fatty Acid Composition of Cultured Human Fibroblasts.  J. Lipid Res., 20:563-547, 1979.
  2. Spector, AA, Hoak, JC, Fry, GL, Denning, GM, Stoll, LL, and Smith, JB.  Effect of Fatty Acid Composition on Prostaglandin Production by Cultured Human Endothelial Cells. J. Clin. Inv., 65:1003-1012, 1980.
  3. Spector, AA, Denning, GM, and Stoll, LL.  Retention of Human Skin Fibroblast Fatty Acid Modifications During Maintenance Culture. In Vitro, 16:932-940,1980.
  4. Denning, GM, Figard, P, and Spector, AA.  Effect of Fatty Acid Modification on Prostaglandin Production by Cultured 3T3 Cells. J. Lipid Res., 23:584-596, 1982.
  5. Yoo, T-J, Kuo, C-Y, Spector, AA, Denning, GM, Floyd, R, Whiteaker, S, Kim, H, Kim, J, Abbas, M, and Budd, TW. Effect of Fatty Acid Modification of Cultured Hepatoma Cells on Susceptability to Natural Killer Cells. Cancer Res., 42:3596-3600, 1982.
  6. Denning, GM, Figard, PH, Kaduce, TL, and Spector, AA.  Role of Triglycerides in Endothelial Cell Arachidonic Acid Metabolism. J. Lipid Res., 24:993-1001, 1983.
  7. Denning, GM, and Fulton, AB.  A Simple Trypsin Resistance Assay for Muscle and Other Cell Fusion. J. Histochem. Cytochem., 34:959-962, 1986.
  8. Denning, GM, Kim, IS, and Fulton, AB.  Shedding of Cytoplasmic Actins by Developing Muscle Cells. J. Cell Sci., 89:273-289, 1988.
  9. Denning, GM, and Fulton, AB.   Purification and Characterization of Clathrin-Coated Vesicles from Chlamydomonas. J. Protozool., 36:334-340, 1989.
  10. Denning, GM, and Fulton, AB.  Electron Microscopy of a Contractile-Vacuole Mutant of Chlamydomonas moewusii (Chlorophyta) defective in the late stages of Diastole. J. Phycol., 25:667-672, 1989.
  11. DeLisle, S, Krause, K-H, Denning, G, and Welsh, MJ. Effect of Inositol Trisphosphate and Calcium on Oscillating Elevations of Intracellular Calcium in Xenopus Oocytes. J. Biol. Chem., 265:11726-11730, 1990.
  12. Denning, GM, and Welsh, MJ. Polarized Distribution of Bradykinin Receptors on Airway Epithelial Cells and Independent Coupling to Second Messenger Pathways. J. Biol. Chem., 266:12932-12938, 1991.
  13. Denning, GM, Ostedgaard, LS, Cheng, SH, Smith, AE, and Welsh, MJ.  Localization of Cystic Fibrosis Transmembrane Conductance Regulator in Chloride Secretory Epithelia. J. Clin. Inv., 89:339-349, 1992. 
  14. Denning, GM, Ostedgaard, LS, and Welsh, MJ.  Abnormal Localization of Cystic Fibrosis Transmembrane Conductance Regulator in Primary Cultures of Cystic Fibrosis Airway Epithelia. J. Cell Biol., 118 :551-559, 1992.
  15. Denning, GM, Anderson, MP, Amara, JF, Marshall, J, Smith, AE, and Welsh, MJ: Processing of Mutant Cystic Fibrosis Transmembrane Conductance Regulator is Temperature-Sensitive. Nature, 358:761-764, 1992.
  16. McCray, PB, Bettencourt, JD, Bastacky, J, Denning, G, and Welsh, MJ.  Expression of CFTR and a cAMP-Stimulated Chloride Secretory Pathway in Cultured Human Fetal Alveolar Epithelial Cells. Am. J. Resp. Cell Mol. Biol., 9:578-585, 1993.
  17. Welsh, M.J., Denning, G.M., Ostedgaard, L.S., and Anderson, M.P.  Dysfunction of CFTR Bearing the ∆F508 Mutation.  J. Cell Sci., 17:235-239, 1993.
  18. Sheppard, DN, Carson, MR, Ostedgaard, LS, Denning, GM, and Welsh, MJ. Expression of Cystic Fibrosis Transmembrane Conductance Regulator in a Model Epithelium. Am. J. Physiol., 266:L405-L413, 1994.
  19. Githens, S, Schexnayder, JA, Moses, RL, Denning, GM, Smith, JJ, and Frazier, ML. Mouse Pancreatic Acinar/Ductular Tissue Gives Rise to Epithelial Cultures that are Morphologically, Biochemically, and Functionally Indistinguishable from Interlobular Duct Cell Cultures.  In Vitro Cell. Devel. Biol., 30A:622-635, 1994.
  20. Stoll, LL, Denning, GM, Kasner, NA, and Hunninghake, GW. Platelet-Activating Factor may Stimulate Both Receptor-Dependent, and Receptor-Independent Increases in [Ca2+] in Human Airway Epithelial Cells. J. Biol. Chem., 269:4254-4259, 1994.
  21. Welsh, M.J., Anderson, M.P., Rich, D.P., Berger, H.A., Denning, G.M., Ostedgaard, L.S., and Sheppard, D.N. Abnormalities of Airway Epithelial Chloride Transport in Cystic Fibrosis.  In Airway Secretion. Tamotsu Takishima and Sanae Shimura (eds). Marcel Dekker, Inc., New York, 1994.
  22. Denning, GM and Welsh, MJ. Cyclic-AMP and Inositol 1,4,5-Trisphosphate Increase Calcium in HT29 Cells by Activating Different Ca2+ Influx Pathways. Am. J. Physiol.,267:C776-C783, 1994.
  23. Denning, GM.  Interleukin-4 and Interferon-g Synergistically Increase Total Polymeric IgA Receptor Levels in Human Intestinal Epithelial Cells: Role of Protein Tyrosine Kinases. J. Immunol., 156:4807-4814, 1996.
  24. Denning, GM, Leidal, KG, Holst, VA, Iyer, SS, Pearson, DW, Clark, JR, Nauseef, WM, and Clark, RA. Calreticulin Biosynthesis and Processing in Human Myeloid Cells: Demonstration of Signal Peptide Cleavage and N-glycosylation. Blood 90:372-381, 1997.
  25. Denning, GM, Railsback, MA, Rasmussen, GT, Cox, CD, and Britigan, BE Pseudomonas Pyocyanine Alters Calcium Signaling in Human Airway Epithelial Cells. Am. J. Physiol. 274:L893-L900, 1998
  26. Denning, GM, Wollenweber, LA, Railsback, MA, Cox, CD, Stoll, LL, and Britigan, BE Pseudomonas Pyocyanin Increases Interleukin-8 Release by Human Airway Epithelial Cells. Infect. Immun. 66:5777-5784, 1998.
  27. Ackermann, LA, Wollenweber, LA and Denning, GM. IL-4 and IFN-g Increase Steady State Levels of pIgR in Human Airway and Intestinal Epithelial Cells. J. Immunol. 162:5112-5118, 1999.
  28. Malik, ZA, Denning, GM, and Kusner, DJ. Inhibition of Ca2+-signaling by Mycobacterium tuberculosis is associated with reduced phagosome-lysosome fusion and increased survival within macrophages. J. Exp. Med., 191:287-302, 2000.
  29. McCormick, ML, Denning, GM, Reszka, KJ, Bilski, P, Buettner, GR, Rasmussen, GT, Railsback, MA., and Britigan, BE. Biological effects of menadione photochemistry: Effects of menadione on biological systems may not involve classical oxidant production. Biochem. J. 350: 797-804, 2000.
  30. Leidal, KG, Munson, K, Johnson, M, and Denning, GM. Small molecular weight factors from Pseudomonas aeruginosa have opposite effects on IL-8 and RANTES release by human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 25:186-195, 2001.
  31. Clark, RA, Li SL, Pearson, DW, Leidal, KG, Clark, JR, Denning, GM, Reddick, R, Krause, K-H, and Valente, AJ. Regulation of calreticulin expression during induction of differentiation in human myeloid cells:  Evidence for remodeling of the endoplasmic reticulum. J. Biol. Chem. 277:32369-32378, 2002.
  32. Rooney, CP, Denning, GM, Davis, BP, Flaherty, DM, Chiorini, JA, and Zabner, J. Bronchoalveolar fluid is not a major hindrance to viral mediated gene therapy in cystic fibrosis. J. Virol.. 76(20):10437-10443, 2002.
  33. O’Malley, Y.Q., Abdalla, M.Y., McCormick, M.L., Reszka, K.J., Denning G.M. and Britigan, B.E. Subcellular Localization of Pseudomonas Pyocyanin Cytotoxicity in Human Lung Epithelial Cells. Am. J. Physiol. 284:L420-L430, 2003.
  34. Leidal, KG, Munson, KL, Johnson, M, and Denning, GM. Metalloproteases from Pseudomonas aeruginosa-degrade human RANTES, MCP-1 and ENA-78. J. Interfer. Cyt. Res. 23:307-318, 2003.
  35. Denning, GM, Iyer, SS, Reszka, KJ, O’Malley, Y, Rasmussen, GT, and Britigan, BE. Phenazine-1-carboxylic acid, a secondary metabolite of Pseudomonas aeruginosa, alters expression of immunomodulatory proteins by human airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Biol. 285:L584-L592, 2003.
  36. O’Malley, YQ, Reszka, KJ, Rasmussen, GT, Abdalla, MY, Denning, GM, and Britigan, BE. The Pseudomonas secretory product pyocyanin inhibits catalase activity in human lung epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol.285:L1077-L1086, 2003.
  37. Rice, JB, Stoll, LL, Li, W-G, Denning, GM, Weydert, J, Charipar, E, Richenbacher, WE, Miller Jr., FJ, and Weintraub, NL. Low level endotoxin induces potent inflammatory activation of human blood vessels: Inhibition by statins. Arterioscler. Thromb. Vasc. Biol. 23:1576-1582, 2003.
  38. Ackermann, LA, Munson, KL, and Denning, GM. NF-kB contributes to both IL-4- and IFN-dependent expression of the polymeric immunoglobulin receptor. Immunology 111:75-85, 2004.
  39. Harrelson, A, Denning, GM, Romig, SA, Weintraub, NL, and Stoll, LL. Functional expression of membrane-bound CD14 (mCD14) in human coronary artery smooth muscle cells is down regulated by rosaglitazone. FASEB J., 18:A192, 2004.
  40. Reszka, KJ, O’Malley, Y, McCormick, ML, Denning, GM, and Britigan, BE. Oxidation of pyocyanin, a cytotoxic product from Pseudomonas aeruginosa, by microperoxidase 11 and hydrogen peroxide. Free Rad. Biol. Med. 36(11): 1448-1459, 2004.
  41. Stoll, LL, McCormick, ML, Denning, GM, and Weintraub, NL. Antioxidant effects of statins. Drugs of Today. 40(12): 975-90, 2004.
  42. Stoll, LL, Denning, GM, Weintraub, NL. Potential role of endotoxin as a proinflammatory mediator of atherosclerosis. Arterioscler Thronb Vasc Biol, 24:2227-2236, 2004.
  43. Stoll, LL, Denning, GM, Li, W-G, Rice, JB, Jones, AD, Gunnlaugsson, ST, Miller Jr., FJ, Hohl, RJ, and Weintraub, NL. Regulation of endotoxin-induced pro-inflammatory activation in human coronary artery endothelial and smooth muscle cells. J. Immunol., 173:1336-1343, 2004.
  44. O’Malley, YQ, Reszka, KJ, Spitz, DR, Denning, GM, and Britigan, BE. Pseudomonas aeruginosa pyocyanin directly oxidizes glutathione and decreases its levels in airway epithelial cells. Am. J Physiol 287:L94-L103, 2004.
  45. Look, DC, Stoll, LL, Romig, SA, Humlicek, A, Britigan, BE and Denning, GM. Pyocyanin and its precursor phenazine-1-carboxylic acid increase IL-8 and intercellular adhesion molecule-1 expression in human airway epithelial cells by oxidant-dependent mechanisms. J. Immunol. 1775(6):4017-4023, 2005.
  46. Beharka, A.A., Crowther, J.E., McCormack, F.X., Denning, G.M., Lees, J., Tibesar, E., Schlesinger, L.S. Pulmonary surfactant protein A activates a phosphatidylinositol 3-kinase/calcium signal transduction pathway in human macrophages: participation in the up-regulation of mannose receptor activity. J. Immunol. 175:2227-2236, 2005.
  47. Stoll, LL, McCormick, ML, Denning, GM, Weintraub, NL. Antioxidant effects of statins. Timely Top Med Cardiovasc Dis. 9:E1, 2005.
  48. Reszka, KJ, Denning, GM and Britigan, BE. Photosensitized oxidation and inactivation of pyocyanin, a virulence factor of Pseudomonas aeruginosa. Photochemistry and Photobiology, 82:466-473, 2006.
  49. Denning, GM and Stoll, LL. Peroxisome proliferator-activated receptors: Potential therapeutic targets in lung disease (Review). Pediatric Pulmonology, 41:23-34, 2006.
  50. Dickson, EW, Ludwig, PS, Ackermann, LW, Buresh, CT and Denning, GM. Met-enkephalin-Arg-Phe (MEAP): A cardioprotective hormonal opioid. Acad. Emerg. Med. 18:813-819, 2006.
  51. Stoll, LL, Denning, GM and Weintraub, NL. Endotoxin, TLR4 signaling and vascular inflammation: Potential therapeutic targets in cardiovascular disease (Review). Current Pharmaceutical Design, 12(32):4229-4245, 2006.
  52. Dickson, EW, Hogrefe, CP, Ludwig, PS, Ackermann, LW, Stoll, LL, Denning, GM. Exercise Enhances Myocardial Ischemic Tolerance via an Opioid Receptor-Dependent Mechanism, Am J Physiol Heart Circ Physiol. 294:H402-H408, 2008.
  53. Denning, GM, Ackermann, LW, Barna, TJ, Armstrong, JG, Stoll, LL, Weintraub, NL, and Dickson, EW. Proenkephalin expression and enkephalin release are widely observed in non-neuronal tissues. Peptides 29:83-92, 2008.
  54. Chatterjee, T. K. Stoll, L. L., Denning, G. M., Harrelson, A., Blomkalns, A. L., Idelman, G., Rothenberg, F. G., Neltner, B., Romig-Martin, S. A., Dickson, E. W., Rudich, S., Weintraub, N. L. Proinflammatory phenotype of perivascular adipocytes: influence of high-fat feeding. Circ Res, 104(4):541-549, 2009.
  55. Cheluvappa, R, Denning, GM, Lau, GW, Grimm, MC, Hilmer, SN, Le Couteur, DG. Pseudomonas aeruginosa toxins and pathogenesis of the hyperlipidaemia of sepsis. Pathology. 41(7):615-621, 2009.
  56. Cheluvappa, R., Denning, G.M., Lau, G.W., Grimm, M.C., Hilmer, S.N., and D.G Le Couteur. Pathogenesis of the hyperlipidemia of Gram-negative bacterial sepsis may involve pathomorphological changes in liver sinusoidal endothelial cells. Int J Infect Dis 14(10):e857-e867, 2010.
  57. Vaughn, L.K., Denning, G., Stuhr, K.L., de Wit, H., Hill, M.N., and Hillard, C.J. Endocannabinoid signaling: has it got rhythm? Themed Issue: Cannabinoids. British Journal of Pharmacology. 160:530-543, 2010.
  58. Otis, JP, Ackermann, LW, Denning, GM, and Carey, HV. Identification of qRT-PCR reference genes for analysis of opioid gene expression in a hibernator. Journal of Comparative Physiology B, 180(4):619-629, 2010.
  59. Blomkalns, AL, Gavrila, D, Thomas, N, Nelter, BS, McCormick, ML, Stoll, LL, Denning, GM, Collins, SP, Qin, Z, Daugherty, A, Cassis, LA, Thompson, RW, Weiss, RM, Lindower, PD, Pinney, SM, Chaterjee, T, and Weintraub, NL. Absence of CD14 attenuates abdominal aortic aneurysm formation: Relevance to human aneurismal disease. J Inflammation 2011.