Luke A. Wiley, PhD

Portrait
Research Assistant Professor of Ophthalmology and Visual Sciences

Contact Information

Lab: 4156 Medical Education & Research Facility
375 Newton Rd.
Iowa City, IA 52242
319-335-7242

Education

BS, Bioengineering, University of Illinois at Urbana-Champaign
PhD, Molecular, Cellular and Systemic Physiology, Southern Illinois University-Carbondale

Post Doctoral Fellow, Ophthalmology & Visual Sciences: Eye development, Washington University
Post Doctoral Fellow, Ophthalmology & Visual Sciences: Retinal immunity, Washington University

Center, Program and Institute Affiliations

Institute for Vision Research

Research Summary

At the Institute for Vision Research at the University of Iowa, I am using my years of experience throughout the eye in cell, molecular biology, developmental and immune biology to use patient-derived iPSCs to model rare inherited eye diseases, including Batten disease, Stargardt disease, Leber congenital amaurosis, enhanced S-cone syndrome, retinitis pigmentosa, and North Carolina Macular Dystrophy. Furthermore, using my background in immune biology, we are testing how various genetic and immune backgrounds affect the transplantation of patient-derived iPSCs. We will use the knowledge gained from these studies as we proceed towards the prime directive of transplanting autologous iPSCs back into diseased patient retinas to restore functional vision. The WIVR now has a fully operational FDA-licensed cGMP facility, the Steven W. Dezii Translational Vision Research Facility. I also serve as the Director of Functional Analysis for DTVRF, assisting in the generation of clinical-grade adeno-associated viral vectors and transplantable human photoreceptor precursor cells that will be used for gene and cell replacement therapies in the very near future for patients with blinding inherited eye disease.

Publications

Sharma, T. P., Wiley, L. A., Whitmore, S. S., Anfinson, K. R., Cranston, C. M., Oppedal, D. J., Daggett, H. T., Mullins, R. F., Tucker, B. A. & Stone, E. M. (2017). Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa. Stem cell research, 21, 58-70. PMID: 28390992.

Wiley, L. A., Anfinson, K. R., Cranston, C. M., Kaalberg, E. E., Collins, M. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2017). Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy. Curr Protoc Stem Cell Biol, 42, 4A.12.1-4A.12.14. PMID: 28806854.

Burnight, E. R., Gupta, M., Wiley, L. A., Anfinson, K. R., Tran, A., Triboulet, R., Hoffmann, J. M., Klaahsen, D. L., Andorf, J. L., Jiao, C., Sohn, E. H., Adur, M. K., Ross, J. W., Mullins, R. F., Daley, G. Q., Schlaeger, T. M., Stone, E. M. & Tucker, B. A. (2017). Using CRISPR-Cas9 to Generate Gene-Corrected Autologous iPSCs for the Treatment of Inherited Retinal Degeneration. Mol Ther, 25(9), 1999-2013. PMID: 28619647.

Worthington, K. S., Wiley, L. A., Kaalberg, E. E., Collins, M. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2017). Two-photon polymerization for production of human iPSC-derived retinal cell grafts. Acta Biomater, 55, 385-395. PMID: 28351682.

Small, K. W., DeLuca, A. P., Whitmore, S. S., Rosenberg, T., Silva-Garcia, R., Udar, N., Puech, B., Garcia, C. A., Rice, T. A., Fishman, G. A., Héon, E., Folk, J. C., Streb, L. M., Haas, C. M., Wiley, L. A., Scheetz, T. E., Fingert, J. H., Mullins, R. F., Tucker, B. A. & Stone, E. M. (2016). North Carolina Macular Dystrophy Is Caused by Dysregulation of the Retinal Transcription Factor PRDM13. Ophthalmology, 123(1), 9-18. PMID: 26507665.

Wiley, L. A., Burnight, E. R., DeLuca, A. P., Anfinson, K. R., Cranston, C. M., Kaalberg, E. E., Penticoff, J. A., Affatigato, L. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness. Scientific reports, 6, 30742. PMID: 27471043.

Worthington, K. S., Wiley, L. A., Mullins, R. F., Tucker, B. A. & Nuxoll, E. (2016). Prevascularized silicon membranes for the enhancement of transport to implanted medical devices. Journal of biomedical materials research. Part B, Applied biomaterials, 104(8), 1602-1609. PMID: 26316050.

Wiley, L. A., Beebe, D. C., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). A Method for Sectioning and Immunohistochemical Analysis of Stem Cell-Derived 3-D Organoids. Current protocols in stem cell biology, 37, 1C.19.1-1C.19.11. PMID: 27171793.

Santeford, A., Wiley, L. A., Park, S., Bamba, S., Nakamura, R., Gdoura, A., Ferguson, T. A., Rao, P. K., Guan, J. L., Saitoh, T., Akira, S., Xavier, R., Virgin, 4th, H. W. & Apte, R. S. (2016). Impaired autophagy in macrophages promotes inflammatory eye disease. Autophagy, 12(10), 1876-1885. PMID: 27463423.

Giacalone, J. C., Wiley, L. A., Burnight, E. R., Songstad, A. E., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). Concise Review: Patient-Specific Stem Cells to Interrogate Inherited Eye Disease. Stem cells translational medicine, 5(2), 132-40. PMID: 26683869.

DeLuca, A. P., Whitmore, S. S., Barnes, J., Sharma, T. P., Westfall, T. A., Scott, C. A., Weed, M. C., Wiley, J. S., Wiley, L. A., Johnston, R. M., Schnieders, M. J., Lentz, S. R., Tucker, B. A., Mullins, R. F., Scheetz, T. E., Stone, E. M. & Slusarski, D. C. (2016). Hypomorphic mutations in TRNT1 cause retinitis pigmentosa with erythrocytic microcytosis. Human molecular genetics, 25(1), 44-56. PMID: 26494905.

Zeng, S., Whitmore, S. S., Sohn, E. H., Riker, M. J., Wiley, L. A., Scheetz, T. E., Stone, E. M., Tucker, B. A. & Mullins, R. F. (2016). Molecular response of chorioretinal endothelial cells to complement injury: implications for macular degeneration. The Journal of pathology, 238(3), 446-56. PMID: 26564985.

Worthington, K. S., Green, B. J., Rethwisch, M., Wiley, L. A., Tucker, B. A., Guymon, C. A. & Salem, A. K. (2016). Neuronal Differentiation of Induced Pluripotent Stem Cells on Surfactant Templated Chitosan Hydrogels. Biomacromolecules, 17(5), 1684-95. PMID: 27008004.

Worthington, K. S., Wiley, L. A., Guymon, C. A., Salem, A. K. & Tucker, B. A. (2016). Differentiation of Induced Pluripotent Stem Cells to Neural Retinal Precursor Cells on Porous Poly-Lactic-co-Glycolic Acid Scaffolds. Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 32(5), 310-6. PMID: 26692377.

Wiley, L. A., Kaalberg, E. E., Penticoff, J. A., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). Expression of the retina-specific flippase, ABCA4, in epidermal keratinocytes. F1000Research, 5, article number 193. DOI: 10.12688/f1000research.8089.1.

Wiley, L. A., Burnight, E. R., Drack, A. V., Banach, B. B., Ochoa, D., Cranston, C. M., Madumba, R. A., East, J. S., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2016). Using Patient-Specific Induced Pluripotent Stem Cells and Wild-Type Mice to Develop a Gene Augmentation-Based Strategy to Treat CLN3-Associated Retinal Degeneration. Hum Gene Ther, 27(10), 835-846. PMID: 27400765.

Wiley, L. A., Burnight, E. R., Songstad, A. E., Drack, A. V., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2015). Patient-specific induced pluripotent stem cells (iPSCs) for the study and treatment of retinal degenerative diseases. Progress in retinal and eye research, 44, 15-35. PMID: 25448922.

Songstad, A. E., Wiley, L. A., Duong, K., Kaalberg, E., Flamme-Wiese, M. J., Cranston, C. M., Riker, M. J., Levasseur, D., Stone, E. M., Mullins, R. F. & Tucker, B. A. (2015). Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration. Investigative ophthalmology & visual science, 56(13), 8258-67. PMID: 26720480.

Wiley, L. A., Burnight, E. R., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2014). Stem cells as tools for studying the genetics of inherited retinal degenerations. Cold Spring Harbor perspectives in medicine, 5(5), a017160. PMID: 25502747.

Burnight, E. R., Wiley, L. A., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2014). Gene therapy using stem cells. Cold Spring Harbor perspectives in medicine, 5(4). PMID: 25395375.

Worthington, K. S., Wiley, L. A., Bartlett, A. M., Stone, E. M., Mullins, R. F., Salem, A. K., Guymon, C. A. & Tucker, B. A. (2014). Mechanical properties of murine and porcine ocular tissues in compression. Experimental eye research, 121, 194-9. PMID: 24613781.

Burnight, E. R., Wiley, L. A., Drack, A. V., Braun, T. A., Anfinson, K. R., Kaalberg, E. E., Halder, J. A., Affatigato, L. M., Mullins, R. F., Stone, E. M. & Tucker, B. A. (2014). CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype. Gene therapy, 21(7), 662-72. PMID: 24807808.

Oladipupo, S. S., Smith, C., Santeford, A., Park, C., Sene, A., Wiley, L. A., Osei-Owusu, P., Hsu, J., Zapata, N., Liu, F., Nakamura, R., Lavine, K. J., Blumer, K. J., Choi, K., Apte, R. S. & Ornitz, D. M. (2014). Endothelial cell FGF signaling is required for injury response but not for vascular homeostasis. Proceedings of the National Academy of Sciences of the United States of America, 111(37), 13379-84. PMID: 25139991.

Tucker, B. A., Solivan-Timpe, F., Roos, B. R., Anfinson, K. R., Robin, A. L., Wiley, L. A., Mullins, R. F. & Fingert, J. H. (2014). Duplication of TBK1 Stimulates Autophagy in iPSC-derived Retinal Cells from a Patient with Normal Tension Glaucoma. Journal of stem cell research & therapy, 3(5), 161. PMID: 24883232.

Fu, M., Landreville, S., Agapova, O. A., Wiley, L. A., Shoykhet, M., Harbour, J. W. & Heuckeroth, R. O. (2013). Retinoblastoma protein prevents enteric nervous system defects and intestinal pseudo-obstruction. The Journal of clinical investigation, 123(12), 5152-64. PMID: 24177421.

Wiley, L., Bridge, D. R., Wiley, L. A., Odom, J. V., Elliott, T. & Olson, J. C. (2012). Bacterial biofilm diversity in contact lens-related disease: emerging role of Achromobacter, Stenotrophomonas, and Delftia. Investigative ophthalmology & visual science, 53(7), 3896-905. PMID: 22589441.

Wiley, L. A., Rajagopal, R., Dattilo, L. K. & Beebe, D. C. (2011). The tumor suppressor gene Trp53 protects the mouse lens against posterior subcapsular cataracts and the BMP receptor Acvr1 acts as a tumor suppressor in the lens. Disease models & mechanisms, 4(4), 484-95. PMID: 21504908.

Wiley, L. A., Dattilo, L. K., Kang, K. B., Giovannini, M. & Beebe, D. C. (2010). The tumor suppressor merlin is required for cell cycle exit, terminal differentiation, and cell polarity in the developing murine lens. Investigative ophthalmology & visual science, 51(7), 3611-8. PMID: 20181838.

Wiley, L. A., Shui, Y. B. & Beebe, D. C. (2010). Visualizing lens epithelial cell proliferation in whole lenses. Molecular vision, 16, 1253-9. PMID: 20664699.

Wiley, L. A., Berkowitz, B. A. & Steinle, J. J. (2006). Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina. Investigative ophthalmology & visual science, 47(1), 439-43. PMID: 16384994.

Wiley, L. A., Rupp, G. R. & Steinle, J. J. (2005). Sympathetic innervation regulates basement membrane thickening and pericyte number in rat retina. Investigative ophthalmology & visual science, 46(2), 744-8. PMID: 15671308.