Markus H. Kuehn, PhD

Portrait
Professor of Ophthalmology and Visual Sciences

Contact Information

Primary Office
4120F MERF
Iowa City, IA 52242
319-335-9565

Education

MS, Botany, Goethe University (Frankfurt)
PhD, Cell and Molecular Biology, St. Louis University
Postdoctoral Fellow, Ophthalmology, The University of Iowa

Education/Training Program Affiliations

Interdisciplinary Graduate Program in Genetics, Interdisciplinary Graduate Program in Neuroscience

Center, Program and Institute Affiliations

Institute for Vision Research

Research Summary

My laboratory studies the cellular and genetic mechanisms that are involved in glaucoma. Toward this end we have developed a variety of techniques that can be used to evaluate ocular phenotypes in animal models of the disease and have developed extensive expertise in the analysis of genetic data. While the majority of our work is carried out either human samples or rodent models we have developed substantial experience working with both cat and dog models of glaucoma.

Publications

Kuehn, M. H., Mishra, R., Deonovic, B. E., Miller, K. N., McCormack, S. E., Liu, G. T., Kupersmith, M. J. & Wall, M. (In Press). Genetic Survey of Adult-Onset Idiopathic Intracranial Hypertension. Journal of neuro-ophthalmology. PMID: 29608535.

Keller, K. E., Bhattacharya, S. K., Borrás, T., Brunner, T. M., Chansangpetch, S., Clark, A. F., Dismuke, W. M., Du, Y., Elliott, M. H., Ethier, C. R., Faralli, J. A., Freddo, T. F., Fuchshofer, R., Giovingo, M., Gong, H., Gonzalez, P., Huang, A., Johnstone, M. A., Kaufman, P. L., Kelley, M. J., Knepper, P. A., Kopczynski, C. C., Kuchtey, J. G., Kuchtey, R. W., Kuehn, M. H., Lieberman, R. L., Lin, S. C., Liton, P., Liu, Y., Lütjen-Drecoll, E., Mao, W., Masis-Solano, M., McDonnell, F., McDowell, C. M., Overby, D. R., Pattabiraman, P. P., Raghunathan, V. K., Rao, P. V., Rhee, D. J., Chowdhury, U. R., Russell, P., Samples, J. R., Schwartz, D., Stubbs, E. B., Tamm, E. R., Tan, J. C., Toris, C. B., Torrejon, K. Y., Vranka, J. A., Wirtz, M. K., Yorio, T., Zhang, J., Zode, G. S., Fautsch, M. P., Peters, D. M., Acott, T. S. & Stamer, W. D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Experimental eye research, 171, 164-173. PMID: 29526795.

Muenster, S., Lieb, W. S., Fabry, G., Allen, K. N., Kamat, S. S., Guy, A. H., Dordea, A. C., Teixeira, L., Tainsh, R. E., Yu, B., Zhu, W., Ashpole, N. E., Malhotra, R., Brouckaert, P., Bloch, D. B., Scherrer-Crosbie, M., Stamer, W. D., Kuehn, M. H., Pasquale, L. R. & Buys, E. S. (2017). The Ability of Nitric Oxide to Lower Intraocular Pressure Is Dependent on Guanylyl Cyclase. Investigative ophthalmology & visual science, 58(11), 4826-4835. PMID: 28973329.

Zhu, W., Jain, A., Gramlich, O. W., Tucker, B. A., Sheffield, V. C. & Kuehn, M. H. (2017). Restoration of Aqueous Humor Outflow Following Transplantation of iPSC-Derived Trabecular Meshwork Cells in a Transgenic Mouse Model of Glaucoma. Investigative ophthalmology & visual science, 58(4), 2054-2062. PMID: 28384726.

Williams, P. A., Marsh-Armstrong, N. & Howell, G. R. (2017). Neuroinflammation in glaucoma: A new opportunity. Experimental eye research, 157, 20-27. PMID: 28242160.

Chirco, K. R., Hazlewood, R. J., Miller, K., Workalemahu, G., Jampol, L. M., Lesser, G. R., Mullins, R. F., Kuehn, M. H. & Fingert, J. H. (2016). MMP19 expression in the human optic nerve. Molecular vision, 22, 1429-1436. PMID: 28003733.

Lavik, E., Kuehn, M. H., Shoffstall, A. J., Atkins, K., Dumitrescu, A. V. & Kwon, Y. H. (2016). Sustained Delivery of Timolol Maleate for Over 90 Days by Subconjunctival Injection. Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 32(10), 642-649. PMID: 27835065.

Kuehn, M. H., Lipsett, K. A., Menotti-Raymond, M., Whitmore, S. S., Scheetz, T. E., David, V. A., O'Brien, S. J., Zhao, Z., Jens, J. K., Snella, E. M., Ellinwood, N. M. & McLellan, G. J. (2016). Correction: A Mutation in LTBP2 Causes Congenital Glaucoma in Domestic Cats (Felis catus). PloS one, 11(8), e0161517. PMID: 27537365.

Zhu, W., Gramlich, O. W., Laboissonniere, L., Jain, A., Sheffield, V. C., Trimarchi, J. M., Tucker, B. A. & Kuehn, M. H. (2016). Transplantation of iPSC-derived TM cells rescues glaucoma phenotypes in vivo. Proceedings of the National Academy of Sciences of the United States of America, 113(25), E3492-500. PMID: 27274060.

Gramlich, O. W., Burand, A. J., Brown, A. J., Deutsch, R. J., Kuehn, M. H. & Ankrum, J. A. (2016). Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy. Scientific reports, 6, 26463. PMID: 27212469.

Sohn, E. H., van Dijk, H. W., Jiao, C., Kok, P. H., Jeong, W., Demirkaya, N., Garmager, A., Wit, F., Kucukevcilioglu, M., van Velthoven, M. E., DeVries, J. H., Mullins, R. F., Kuehn, M. H., Schlingemann, R. O., Sonka, M., Verbraak, F. D. & Abramoff, M. D. (2016). Retinal neurodegeneration may precede microvascular changes characteristic of diabetic retinopathy in diabetes mellitus. Proceedings of the National Academy of Sciences USA, 113(19), E2655-E2664. PMID: 27114552.

Kuehn, M. H., Lipsett, K. A., Menotti-Raymond, M., Whitmore, S. S., Scheetz, T. E., David, V. A., O'Brien, S. J., Zhao, Z., Jens, J. K., Snella, E. M., Ellinwood, N. M. & McLellan, G. J. (2016). A Mutation in LTBP2 Causes Congenital Glaucoma in Domestic Cats (Felis catus). PloS one, 11(5), e0154412. PMID: 27149523.

Ahram, D. F., Alward, W. L. & Kuehn, M. H. (2015). The genetic mechanisms of primary angle closure glaucoma. Eye (London, England), 29(10), 1251-1259. PMID: 26206529.

Gramlich, O. W., Ding, Q. J., Zhu, W., Cook, A., Anderson, M. G. & Kuehn, M. H. (2015). Adoptive transfer of immune cells from glaucomatous mice provokes retinal ganglion cell loss in recipients. Acta neuropathologica communications, 3, 56. PMID: 26374513.

Yang, X., Hondur, G., Li, M., Cai, J., Klein, J. B., Kuehn, M. H. & Tezel, G. (2015). Proteomics Analysis of Molecular Risk Factors in the Ocular Hypertensive Human Retina. Investigative ophthalmology & visual science, 56(10), 5816-30. PMID: 26348630.

Zode, G. S., Kuehn, M. H., Nishimura, D. Y., Searby, C. C., Mohan, K., Grozdanic, S. D., Bugge, K., Anderson, M. G., Clark, A. F., Stone, E. M. & Sheffield, V. C. (2015). Reduction of ER stress via a chemical chaperone prevents disease phenotypes in a mouse model of primary open angle glaucoma [erratum]. The Journal of clinical investigation, 125(8), 3303. PMID: 26237042.

Ahram, D. F., Grozdanic, S. D., Kecova, H., Henkes, A., Collin, R. W. & Kuehn, M. H. (2015). Variants in Nebulin (NEB) Are Linked to the Development of Familial Primary Angle Closure Glaucoma in Basset Hounds. PloS one, 10(5), e0126660. PMID: 25938837.

Hazlewood, R. J., Roos, B. R., Solivan-Timpe, F., Honkanen, R. A., Jampol, L. M., Gieser, S. C., Meyer, K. J., Mullins, R. F., Kuehn, M. H., Scheetz, T. E., Kwon, Y. H., Alward, W. L., Stone, E. M. & Fingert, J. H. (2015). Heterozygous triplication of upstream regulatory sequences leads to dysregulation of matrix metalloproteinase 19 in patients with cavitary optic disc anomaly. Human mutation, 36(3), 369-78. PMID: 25581579.

Ding, Q. J., Zhu, W., Cook, A. C., Anfinson, K. R., Tucker, B. A. & Kuehn, M. H. (2014). Induction of trabecular meshwork cells from induced pluripotent stem cells. Investigative ophthalmology & visual science, 55(11), 7065-72. PMID: 25298418.

Kuehn, M. H. (2014). Immune phenomena in glaucoma and conformational disorders: why is the second eye not involved?. Journal of glaucoma, 23(8 Suppl 1), S59-61. PMID: 25275909.

Ahram, D. F., Cook, A. C., Kecova, H., Grozdanic, S. D. & Kuehn, M. H. (2014). Identification of genetic loci associated with primary angle-closure glaucoma in the basset hound. Molecular vision, 20, 497-510. PMID: 24791135.

de Fernandez Castro, J. P., Mullins, R. F., Manea, A. M., Hernandez, J., Wallen, T. & Kuehn, M. H. (2013). Lipofuscin in human glaucomatous optic nerves. Experimental eye research, 111, 61-6. PMID: 23567206.

Scheetz, T. E., Fingert, J. H., Wang, K., Kuehn, M. H., Knudtson, K. L., Alward, W. L., Boldt, H. C., Russell, S. R., Folk, J. C., Casavant, T. L., Braun, T. A., Clark, A. F., Stone, E. M. & Sheffield, V. C. (2013). A genome-wide association study for primary open angle glaucoma and macular degeneration reveals novel Loci. PloS one, 8(3), e58657. PMID: 23536807.

Mullins, R. F., Kuehn, M. H., Radu, R. A., Enriquez, G. S., East, J. S., Schindler, E. I., Travis, G. H. & Stone, E. M. (2012). Autosomal recessive retinitis pigmentosa due to ABCA4 mutations: clinical, pathologic, and molecular characterization. Investigative ophthalmology & visual science, 53(4), 1883-94. PMID: 22395892.

Ding, Q. J., Cook, A. C., Dumitrescu, A. V. & Kuehn, M. H. (2012). Lack of Immunoglobulins Does Not Prevent C1q Binding to RGC and Does not Alter the Progression of Experimental Glaucoma. Invest Ophthalmol Vis Sci, 53(10), 6370-7. PMID: 22918632.

Mullins, R. F., Dewald, A. D., Streb, L. M., Wang, K., Kuehn, M. H. & Stone, E. M. (2011). Elevated membrane attack complex in human choroid with high risk complement factor H genotypes. Experimental eye research, 93(4), 565-7. PMID: 21729696.

Sigle, K. J., Camaño-Garcia, G., Carriquiry, A. L., Betts, D. M., Kuehn, M. H. & McLellan, G. J. (2011). The effect of dorzolamide 2% on circadian intraocular pressure in cats with primary congenital glaucoma. Vet Ophthalmol, 14 Suppl 1, 48-53. PMID: 21923823.

Rutz-Mendicino, M. M., Snella, E. M., Jens, J. K., Gandolfi, B., Carlson, S. A., Kuehn, M. H., McLellan, G. J. & Ellinwood, N. M. (2011). Removal of potentially confounding phenotypes from a Siamese-derived feline glaucoma breeding colony. Comp Med, 61(3), 251-7. PMID: 21819695.

Harper, M. M., Grozdanic, S. D., Blits, B., Kuehn, M. H., Zamzow, D., Buss, J. E., Kardon, R. H. & Sakaguchi, D. S. (2011). Transplantation of BDNF-secreting mesenchymal stem cells provides neuroprotection in chronically hypertensive rat eyes. Invest Ophthalmol Vis Sci, 52(7), 4506-15. PMID: 21498611.

Lavik, E., Kuehn, M. H. & Kwon, Y. H. (2011). Novel drug delivery systems for glaucoma. Eye (Lond), 25(5), 578-86. PMID: 21475311.

Kuehn, M. H., Wang, K., Roos, B., Stone, E. M., Kwon, Y. H., Alward, W. L., Mullins, R. F. & Fingert, J. H. (2011). Chromosome 7q31 POAG locus: ocular expression of caveolins and lack of association with POAG in a US cohort. Molecular vision, 17, 430-5. PMID: 21321670.

Trantow, C. M., Cuffy, T. L., Fingert, J. H., Kuehn, M. H. & Anderson, M. G. (2011). Microarray analysis of iris gene expression in mice with mutations influencing pigmentation. Investigative ophthalmology & visual science, 52(1), 237-48. PMID: 20739468.

Yang, X., Luo, C., Cai, J., Powell, D., Yu, D., Kuehn, M. H. & Tezel, G. (2011). Neurodegenerative and Inflammatory Pathway Components Linked to TNF-a/TNFR1 Signaling in the Glaucomatous Human Retina. Invest Ophthalmol Vis Sci, 52(11), 8442-54. PMID: 21917936.

Zode, G. S., Kuehn, M. H., Nishimura, D. Y., Searby, C. C., Mohan, K., Grozdanic, S. D., Bugge, K., Anderson, M. G., Clark, A. F., Stone, E. M. & Sheffield, V. C. (2011). Reduction of ER stress via a chemical chaperone prevents disease phenotypes in a mouse model of primary open angle glaucoma. The Journal of clinical investigation, 121(9), 3542-53. PMID: 21821918.

Luo, C., Yang, X., Kain, A. D., Powell, D. W., Kuehn, M. H. & Tezel, G. (2010). Glaucomatous tissue stress and the regulation of immune response through glial Toll-like receptor signaling. Invest Ophthalmol Vis Sci, 51(11), 5697-707. PMID: 20538986.

Tezel, G., Yang, X., Luo, C., Kain, A. D., Powell, D. W., Kuehn, M. H. & Kaplan, H. J. (2010). Oxidative stress and the regulation of complement activation in human glaucoma. Invest Ophthalmol Vis Sci, 51(10), 5071-82. PMID: 20484586.

Grozdanic, S. D., Lazic, T., Kuehn, M. H., Harper, M. M., Kardon, R. H., Kwon, Y. H., Lavik, E. B. & Sakaguchi, D. S. (2010). Exogenous modulation of intrinsic optic nerve neuroprotective activity. Graefes Arch Clin Exp Ophthalmol, 248(8), 1105-16. PMID: 20229104.

Tezel, G., Yang, X., Luo, C., Cai, J., Kain, A. D., Powell, D. W., Kuehn, M. H. & Pierce, W. M. (2010). Hemoglobin expression and regulation in glaucoma: insights into retinal ganglion cell oxygenation. Invest Ophthalmol Vis Sci, 51(2), 907-19. PMID: 19741249.

Jiang, B., Harper, M. M., Kecova, H., Adamus, G., Kardon, R. H., Grozdanic, S. D. & Kuehn, M. H. (2010). Elevated Expression of Immunomodulatory Genes in Advanced Canine Glaucoma. Mol. Vis, 16, 2092-2108.

Grozdanic, S. D., Kecova, H., Harper, M. M., Nilaweera, W., Kuehn, M. H. & Kardon, R. H. (2010). Functional and structural changes in a canine model of hereditary primary angle-closure glaucoma. Invest Ophthalmol Vis Sci, 51(1), 255-63. PMID: 19661222.

Lively, G. D., Jiang, B., Hedberg-Buenz, A., Chang, B., Petersen, G. E., Wang, K., Kuehn, M. H. & Anderson, M. G. (2010). Genetic dependence of central corneal thickness among inbred strains of mice. Investigative ophthalmology & visual science, 51(1), 160-71. PMID: 19710407.

Jiang, B., Harper, M. M., Kecova, H., Adamus, G., Kardon, R. H., Grozdanic, S. D. & Kuehn, M. H. (2010). Neuroinflammation in advanced canine glaucoma. Molecular vision, 16, 2092-108. PMID: 21042562.

Kwon, Y. H., Fingert, J. H., Kuehn, M. H. & Alward, W. L. (2009). Primary open-angle glaucoma. The New England journal of medicine, 360(11), 1113-24. PMID: 19279343.

Kwon, Y. H., Fingert, J. H., Kuehn, M. H., Wallace, L. M. & Alward, W. L. (2009). Mechanisms of Disease: Primary Open-Angle Glaucoma. N. Engl. J. Med, 360(11), 1113-1124.

Tezel, G. (2009). The role of glia, mitochondria, and the immune system in glaucoma. Invest Ophthalmol Vis Sci, 50(3), 1001-1012.

Ostojic, J., Grozdanic, S. D., Syed, N. A., Hargrove, M. S., Trent, J. T., Kuehn, M. H., Kwon, Y. H., Kardon, R. H. & Sakaguchi, D. S. (2008). Patterns of distribution of oxygen-binding globins, neuroglobin and cytoglobin in human retina. Arch Ophthalmol, 126(11), 1530-6. PMID: 19001220.

Ostojic, J., Grozdanic, S., Syed, N. A., Hargrove, M. S., Trent, J. T., Kuehn, M. H., Kardon, R. H., Kwon, Y. H. & Sakaguchi, D. S. (2008). Neuroglobin and cytoglobin distribution in the anterior eye segment: a comparative immunohistochemical study. J Histochem Cytochem, 56(9), 863-72. PMID: 18574250.

Kuehn, M. H., Kim, C. Y., Jiang, B., Dumitrescu, A. V. & Kwon, Y. H. (2008). Disruption of the complement cascade delays retinal ganglion cell death following retinal ischemia-reperfusion. Exp Eye Res, 87(2), 89-95. PMID: 18572163.

Kim, C. Y., Kuehn, M. H., Anderson, M. G. & Kwon, Y. H. (2007). Intraocular pressure measurement in mice: a comparison between Goldmann and rebound tonometry. Eye (London, England), 21(9), 1202-9. PMID: 16946746.

Mullins, R. F., Kuehn, M. H., Faidley, E. A., Syed, N. A. & Stone, E. M. (2007). Differential macular and peripheral expression of bestrophin in human eyes and its implication for best disease. Investigative ophthalmology & visual science, 48(7), 3372-80. PMID: 17591911.

Kuehn, M. H., Kim, C. Y., Ostojic, J., Bellin, M., Alward, W. L., Stone, E. M., Sakaguchi, D. S., Grozdanic, S. D. & Kwon, Y. H. (2006). Retinal synthesis and deposition of complement components induced by ocular hypertension. Experimental eye research, 83(3), 620-8. PMID: 16677633.

Ostojic, J., Sakaguchi, D. S., de Lathouder, Y., Hargrove, M. S., Trent, J. T., Kwon, Y. H., Kardon, R. H., Kuehn, M. H., Betts, D. M. & Grozdanic, S. (2006). Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons. Invest Ophthalmol Vis Sci, 47(3), 1016-23. PMID: 16505036.

Kim, C. Y., Kuehn, M. H., Clark, A. F. & Kwon, Y. H. (2006). Gene expression profile of the adult human retinal ganglion cell layer. Molecular vision, 12, 1640-8. PMID: 17200664.

Mullins, R. F., Skeie, J. M., Malone, E. A. & Kuehn, M. H. (2006). Macular and peripheral distribution of ICAM-1 in the human choriocapillaris and retina. Molecular vision, 12, 224-35. PMID: 16604055.

Kuehn, M. H., Fingert, J. H. & Kwon, Y. H. (2005). Retinal ganglion cell death in glaucoma: Mechanisms and neuroprotective strategies.

Kuehn, M. H., Fingert, J. H. & Kwon, Y. H. (2005). Retinal Ganglion Cell Death in Glaucoma: Mechanisms and Neuroprotective Strategies.

Stone, E. M., Braun, T. A., Russell, S. R., Kuehn, M. H., Lotery, A. J., Moore, P. A., Eastman, C. G., Casavant, T. L. & Sheffield, V. C. (2004). Missense variations in the fibulin 5 gene and age-related macular degeneration. The New England journal of medicine, 351(4), 346-53. PMID: 15269314.

Kuehn, M. H., Stone, E. M. & Hageman, G. S. (2001). Organization of the human IMPG2 gene and its evaluation as a candidate gene in age-related macular degeneration and other retinal degenerative disorders. Investigative ophthalmology & visual science, 42(13), 3123-9. PMID: 11726612.

Alagramam, K. N., Yuan, H., Kuehn, M. H., Murcia, C. L., Wayne, S., Srisailpathy, C. R., Lowry, R. B., Knaus, R., Van Laer, L., Bernier, F. P., Schwartz, S., Lee, C., Morton, C. C., Mullins, R. F., Ramesh, A., Van Camp, G., Hageman, G. S., Woychik, R. P., Smith, R. J. & Hagemen, G. S. (2001). Mutations in the novel protocadherin PCDH15 cause Usher syndrome type 1F. Human molecular genetics, 10(16), 1709-18. PMID: 11487575.

Ruiz, A., Kuehn, M. H., Stone, E. S., Hageman, G. S. & Bok, D. (2001). Characterization of the lecithin retinol acyltransferase (LRAT) gene structure and evaluation of it’s involvement in ocular disease. Invest. Ophthalmol. Vis. Sci, 1, 31-37.

Ruiz, A., Kuehn, M. H., Andorf, J. L., Stone, E., Hageman, G. S. & Bok, D. (2001). Genomic organization and mutation analysis of the gene encoding lecithin retinol acyltransferase in human retinal pigment epithelium. Investigative ophthalmology & visual science, 42(1), 31-7. PMID: 11133845.

Kuehn, M. H., Wietecki, D. T. & Hageman, G. S. (2000). Molecular characterization of the murine orthologue of the human retinal proteoglycan IPM 150. Molecular vision, 6, 148-56. PMID: 10958699.

Kuehn, M. H. (2000). Molecular Characterization of a Novel Family of Extracellular Proteoglycans and Glycoproteins. Dissertation presented to the faculty of the graduate school of Saint Louis University.

Gorin, M. B., Breitner, J. C., De Jong, P. T., Hageman, G. S., Klaver, C. C., Kuehn, M. H. & Seddon, J. M. (1999). The genetics of age-related macular degeneration. Molecular vision, 5, 29. PMID: 10562653.

Kuehn, M. H., Hageman, G. S. (1999). Expression and characterization of the IPM 150 gene (IMPG1) product, a novel human photoreceptor cell-associated chondroitin-sulfate proteoglycan. Matrix biology : journal of the International Society for Matrix Biology, 18(5), 509-18. PMID: 10601738.

Kuehn, M. H., Hageman, G. S. (1999). Molecular characterization and genomic mapping of human IPM 200, a second member of a novel family of proteoglycans. Molecular cell biology research communications : MCBRC, 2(2), 103-10. PMID: 10542133.

Hageman, G. S., Kuehn, M. H. (1998). Biology of the retina-IPM-RPE interface. In M. Marmor , T. Wolfensberger (Eds.) The Pigmented Retinal Epithelium: Current Aspects of Function and Disease. pp. 417-454. Oxford University Press, New York.