Library Prep & Genome Sequencing Fees

NGS Library Prep Fees (per sample)

effective March 1, 2025

Library	Kit	Campus	Off-campus
Illumina mRNA-Seq	Stranded mRNA Prep, Ligation	$137	$149
Illumina Total RNA-seq	Stranded Total RNA Prep, Ligation	$182	$194
KAPA DNA Seq	KAPA HyperPrep (WGS, Other)	$129	$141
Agilent Exome Capture, 1-16 samples	Sure Select, v5 or v6+UTR baits	$355	$408
Agilent Exome Capture, 17+ samples	Sure Select, v5 or v6+UTR baits	$300	$345
Illumina smRNA-seq	TruSeq smRNA	$287	$335
ChIP-Seq	TBD	inquire	inquire
Low coverage WGS (genotyping by sequencing)	TBD	inquire	inquire

Genome Sequencer Fees

effective March 1, 2025

Element AVITI24

Flow Cell	~Reads/Lane	~Yield/Lane	ui cost per lane	ui cost per flow cell*	external cost per lane	external cost per flow cell*
150 cycle (2x75 bp) Cloudbreak FS-High output Cloudbreak FS-Med output Cloudbreak FS-Low output	400-500 M 200-250 M 40-50 M	60-75 GB 30-38 GB 6-7.5 GB	$688 $576 $555	$1,375 $1,151 $1,108	$826 $695 $660	$1,649 $1,388 $1,317

300 cycle (2x150 bp) Cloudbreak FS-High output Cloudbreak FS-Med output Cloudbreak FS-Low output	400-500 M 200-250 M 75-100 M	120-150 GB 60-75 GB 30-38 GB	$1,042 $751 $639	$2,083 $1,501 $1,277	$1,245 $921 $791	$2,488 $1,841 $1,579

600 cycle (2x300 bp) Cloudbreak FS-High output Cloudbreak FS-Med output	250-300 M 75-100 M	150-180 GB 45-60 GB	NA NA	$2,631 $2,013	NA NA	$3,242 $2,540

Flow Cell

~Reads/Lane

~Yield/Lane

ui cost

per lane

ui cost

per flow cell*

external cost

per lane

external cost

per flow cell*

150 cycle (2x75 bp)

Cloudbreak FS-High output

Cloudbreak FS-Med output

Cloudbreak FS-Low output

400-500 M

200-250 M

40-50 M

60-75 GB

30-38 GB

6-7.5 GB

$688

$576

$555

$1,375

$1,151

$1,108

$826

$695

$660

$1,649

$1,388

$1,317

300 cycle (2x150 bp)

Cloudbreak FS-High output

Cloudbreak FS-Med output

Cloudbreak FS-Low output

400-500 M

200-250 M

75-100 M

120-150 GB

60-75 GB

30-38 GB

$1,042

$751

$639

$2,083

$1,501

$1,277

$1,245

$921

$791

$2,488

$1,841

$1,579

600 cycle (2x300 bp)

Cloudbreak FS-High output

Cloudbreak FS-Med output

250-300 M

75-100 M

150-180 GB

45-60 GB

$2,631

$2,013

$3,242

$2,540

*150 and 300 cycle flow cells have two lanes, and the 600 cycle has one.

Illumina NovaSeq 6000

Please inquire about NovaSeq 6000 accessibility and pricing.

Illumina MiSeq

flow cell/run CONFIGURATION	Reads (Clusters)	Yield	Campus Rate	Off-Campus Rate
MiSeq Standard Flow Cell
50 Cycle (50 bp SR / 25 bp PE)	12-15 M	750-850 Mb	$1,254	$1,304
150 Cycle (150 bp SR / 75 bp PE)	22-25M	3.3-3.8 Gb	$1,467	$1,567
300 Cycle (300 bp SR / 150 bp PE)	12-15M	4.5-5.1 Gb	$1,791	$1,891
500 Cycle (250 bp PE)	12-15M	7.5-8.5 Gb	$2,053	$2,253
600 Cycle (300 bp PE)	22-25 M	13.2-15 Gb	$2,547	$2,747
MiSeq Micro Flow Cell
300 Cycle (300 bp SR / 150 bp PE)	4M	1.2 Gb	$689	$739
MiSeq Nano Flow Cell
300 Cycle (300 bp SR / 150 bp PE)	1M	300 Mb	$503	$553
500 Cycle (250 bp PE)	1M	500 Mb	$576	$626

Contact Information:

116 EMRB
Phone: (319) 335-6736
Fax: (319) 335-6737
dna-genomeseq@uiowa.edu

For information about downstream bioinformatics support, contact the IIHG Bioinformatics Division at (319) 335-6717.

Library Prep & Genome Sequencing Fees

NGS Library Prep Fees (per sample)

Library

Kit

Campus

Off-campus

Illumina mRNA-Seq

Stranded mRNA Prep, Ligation

$137

$149

Illumina Total RNA-seq

Stranded Total RNA Prep, Ligation

$182

$194

KAPA DNA Seq

KAPA HyperPrep (WGS, Other)

$129

$141

Agilent Exome Capture, 1-16 samples

Sure Select, v5 or v6+UTR baits

$355

$408

Agilent Exome Capture, 17+ samples

Sure Select, v5 or v6+UTR baits

$300

$345

Illumina smRNA-seq

TruSeq smRNA

$287

$335

ChIP-Seq

TBD

inquire

inquire

Low coverage WGS (genotyping by sequencing)

TBD

inquire

inquire

Genome Sequencer Fees

Element AVITI24

150 cycle (2x75 bp)

300 cycle (2x150 bp)

600 cycle (2x300 bp)

*150 and 300 cycle flow cells have two lanes, and the 600 cycle has one.

Illumina NovaSeq 6000

Illumina MiSeq

Contact Information: