The Next Generation Sequencing Interest Group Meeting is open to everyone. Investigators that are currently performing or are planning to start studies using the Next Gen Sequencing platforms are encouraged to attend. Please contact Kevin Knudtson, PhD (kevin-knudtson@uiowa.edu) if you have questions about the NGS Interest Group. 

Sample preparation for single cell analysis: Basics and beyond

Tuesday, August 30, 2022

Time: 10:00am-2:00pm
Location: Kelch Conference Room, 1289 CBRB

Single cell analysis is a powerful technique to characterize complex tissue types, identify rare cell populations, uncover regulatory relationships between genes, and track cell trajectories. From sample handling to cell dissociation, there are a number of technical considerations that influence cell viability and data quality of single cell genomics, single cell epigenomics, and single cell gene expression experiments. Join us for this seminar, which covers critical sample prep decisions and technical considerations for single cell assay design and optimization.

Agenda:

10:00am	Opening Remarks	Kevin Knudtson, PhD Director, Genomics Division Iowa Institute of Human Genetics
10:05am	Single Cell Workflow and Sample Tech Talk	Kara Pivarski Field Application Specialist 10x Genomics
11:00am	Multimodal Single-Cell Analysis of Non-Random Mitochondrial Heteroplasmy Distribution in the Retina	Nate Mullin MD/PhD Student University of Iowa
11:30am	Break
12:00pm	Spatial Transcriptomics Workflow Tech Talk	Egon Ranghini Senior Science and Technology Advisor 10x Genomics
1:00pm	Spatial Transcriptomics Analysis of Sex Differences in Gene Expression in Mouse Cerebellar Purkinje cells	Wen Wen, PhD Assistant Research Scientist University of Iowa
1:20pm	Visium Spatial Gene Expression data analysis using Partek workflow	Yann Vanrobaeys, PhD Student Neuroscience and Pharmacology Department University of Iowa
1:40pm	Bioinformatics Services at University of Iowa	Michael Chimenti, PhD Director of IIHG Bioinformatics Division University of Iowa