The Next Generation Sequencing Interest Group Meeting is open to everyone. Investigators that are currently performing or are planning to start studies using the Next Gen Sequencing platforms are encouraged to attend. Please contact Kevin Knudtson, PhD (kevin-knudtson@uiowa.edu) if you have questions about the NGS Interest Group.
Sample preparation for single cell analysis: Basics and beyond
Tuesday, August 30, 2022
Time: 10:00am-2:00pm
Location: Kelch Conference Room, 1289 CBRB
Single cell analysis is a powerful technique to characterize complex tissue types, identify rare cell populations, uncover regulatory relationships between genes, and track cell trajectories. From sample handling to cell dissociation, there are a number of technical considerations that influence cell viability and data quality of single cell genomics, single cell epigenomics, and single cell gene expression experiments. Join us for this seminar, which covers critical sample prep decisions and technical considerations for single cell assay design and optimization.
Agenda:
10:00am | Opening Remarks | Kevin Knudtson, PhD Director, Genomics Division Iowa Institute of Human Genetics |
10:05am | Single Cell Workflow and Sample Tech Talk | Kara Pivarski Field Application Specialist 10x Genomics |
11:00am | Multimodal Single-Cell Analysis of Non-Random Mitochondrial Heteroplasmy Distribution in the Retina |
Nate Mullin MD/PhD Student University of Iowa |
11:30am | Break | |
12:00pm | Spatial Transcriptomics Workflow Tech Talk | Egon Ranghini Senior Science and Technology Advisor 10x Genomics |
1:00pm | Spatial Transcriptomics Analysis of Sex Differences in Gene Expression in Mouse Cerebellar Purkinje cells | Wen Wen, PhD Assistant Research Scientist University of Iowa |
1:20pm | Visium Spatial Gene Expression data analysis using Partek workflow | Yann Vanrobaeys, PhD Student Neuroscience and Pharmacology Department University of Iowa |
1:40pm | Bioinformatics Services at University of Iowa | Michael Chimenti, PhD Director of IIHG Bioinformatics Division University of Iowa |