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Next Generation Sequencing Interest Group

The Next Generation Sequencing Interest Group Meeting is open to everyone. Investigators that are currently performing or are planning to start studies using the Next Gen Sequencing platforms are encouraged to attend. Please contact Kevin Knudtson, PhD (kevin-knudtson@uiowa.edu) if you have questions about the NGS Interest Group. 

Sample preparation for single cell analysis: Basics and beyond

Tuesday, August 30, 2022

Time: 10:00am-2:00pm
Location: Kelch Conference Room, 1289 CBRB

Single cell analysis is a powerful technique to characterize complex tissue types, identify rare cell populations, uncover regulatory relationships between genes, and track cell trajectories. From sample handling to cell dissociation, there are a number of technical considerations that influence cell viability and data quality of single cell genomics, single cell epigenomics, and single cell gene expression experiments. Join us for this seminar, which covers critical sample prep decisions and technical considerations for single cell assay design and optimization.


10:00am Opening Remarks Kevin Knudtson, PhD
Director, Genomics Division
Iowa Institute of Human Genetics
10:05am Single Cell Workflow and Sample Tech Talk Kara Pivarski
Field Application Specialist
10x Genomics
11:00am Multimodal Single-Cell Analysis of Non-Random Mitochondrial Heteroplasmy Distribution in the
Nate Mullin
MD/PhD Student
University of Iowa
11:30am Break  
12:00pm Spatial Transcriptomics Workflow Tech Talk Egon Ranghini
Senior Science and Technology Advisor
10x Genomics
1:00pm Spatial Transcriptomics Analysis of Sex Differences in Gene Expression in Mouse Cerebellar Purkinje cells Wen Wen, PhD
Assistant Research Scientist
University of Iowa
1:20pm Visium Spatial Gene Expression data analysis using Partek workflow Yann Vanrobaeys, PhD Student
Neuroscience and Pharmacology Department University of Iowa
1:40pm Bioinformatics Services at University of Iowa Michael Chimenti, PhD
Director of IIHG Bioinformatics Division
University of Iowa