Sanger Sequencing

The IIHG Genomics Division houses 2 Applied Biosystems Model 3730xlu (96-capillary) DNA Sequencers. Sequencing can be performed on single-stranded, double-stranded or PCR-generated templates.

Users must first set up an on-line profile and account with the IIHG Genomics Division. Once an account has been established, Sanger sequencing requests should be made through the on-line ordering system.

Details regarding sample submission can be found here. The user should print their requests and bring the completed request form along with the samples to the division. Tubes must be labeled with 5-digit order number and sample number.

Users will receive email notification when their DNA sequencing results are ready (usually 24 to 48 hours). The email will contain a reminder to login to your account to access the sequencing results that can be downloaded via a web browser through our website. Access to the data files is password protected. This system is NOT HawkID based. You may create or look at your account using the on-line ordering system:

Links to publicly available DNA Sequencing chromatogram trace viewers can be found here.

Contact Information

116 EMRB
Phone: (319) 335-6736
Fax: (319) 335-6737

Samples for sequencing may be dropped off at 116 EMRB anytime from 8:00 AM to 5:00 PM Monday-Friday.