10 Color Flow Cytometry

Scatter Plot

The Immunopathology Laboratory performs several categories of diagnostic testing including autoimmune disease serologies, flow cytometry, immunohistochemistry, and immunofluorescence microscopy.

Autoimmune disease serologies include screening for diseases such as systemic lupus erythematosus (SLE), Sjogren's syndrome, scleroderma, Wegner's granulomatosis and related vasculitides, autoimmune hepatitis, primary biliary cirrhosis, ulcerative colitis, celiac disease and others. Testing methodologies used include enzyme immunoassay direct and indirect immunofluorescence microscopy.

Flow cytometry is done on bone marrow, peripheral blood, fine needle aspirations, miscellaneous body fluids, lymph nodes and solid tumors. Flow cytometry has a wide range of uses from typing lymphomas and leukemias to quantitation of lymphocyte subsets in suspected immunodeficiency diseases.

Ten color Flow Cytometry employs both gating and cluster analysis strategies to maximize the sensitivity and accuracy of the testing.

Immunohistochemistry staining is done primarily on formalin-fixed, paraffin embedded tissues using automated staining equipment. A large number of antibodies are available to immunophenotype and identify the source of tumors and to detect certain infectious agents (particularly viruses) in tissue sections. In-situ hybridization studies are also performed to identify certain types on infections, particularly Epstein-Barr virus.

Immunofluorescence microscopy is done on frozen sections of the skin, mucosae and the kidney to diagnose autoimmune diseases.

How to Send Flow Cytometry Specimens

UIDL Client Services: 
Toll-free: 866-844-2522
Local: 319-384-7212
Fax: 319-384-7213

Hours: Specimens are accepted during our regular business hours.

Reports:  Final result reports may be faxed or provided electronically.

Requisition Form: Please print, fully complete, and send a Comprehensive Hematopathology Requisition with your specimens.

Consultants:
Syrbu
Sergei I. Syrbu, MD, PhD
Director of Flow Cytometry
Office: 6245 Roy Carver Pavilion
Phone: 319-356-7372
Email: sergi-syrbu@uiowa.edu

Bhagavathi Sharathkumar New Faculty Image
Sharathkumar Bhagavathi, MBBS, MD
Office: 6227B Roy Carver Pavilion
Phone: 319-678-8197
Email: sharathkumar-bhagavathi@uiowa.edu

Holman
Carol J. Holman, MD, PhD
Office: 6229 Roy Carver Pavilion
Phone: 319-356-3981
Email: carol-holman@uiowa.edu

Karandikar
Nitin J. Karandikar, MD, PhD
Office: C673 General Hospital
Phone: 319-384-9609
Email: nitin-karandikar@uiowa.edu

Rastogi
Prerna Rastogi, MD, PhD
Office: 5238-F Roy Carver Pavilion
Phone: 319-356-3781
Email: prerna-rastogi@uiowa.edu