Anil K. Chauhan, MTech, PhD

Portrait
Associate Professor of Internal Medicine - Hematology, Oncology and Blood and Marrow Transplantation

Contact Information

Lab: 3120 ML
Iowa City, IA 52242
319-384-4097

Primary Office: 3188 ML
Iowa City, IA 52242
319-335-6525

Education

BSc, Botany, Zoology, Chemistry, University of Delhi
MSc, Biotechnology, Devi Ahilya University
M Tech, Fermentation and Biotechnology, Anna University

Post Doctoral Fellow, Molecular Genetics, International Centre for Genetic Engineering and Biotechnology
Post Doctoral Fellow, Thrombosis and Inflammation, CBR Institute for Biomedical Research
Post Doctoral Fellow, Thrombosis and Inflammation, Immune Disease Institute and Harvard Medical School

Education/Training Program Affiliations

Department of Molecular Physiology and Biophysics PhD, Interdisciplinary Graduate Program in Molecular and Cellular Biology, Medical Scientist Training Program

Center, Program and Institute Affiliations

Holden Comprehensive Cancer Center

Research Summary

Research in laboratory is focused on the role of adhesion molecules in vascular diseases associated with thrombosis and inflammation. By using state-of-the-art technique, intravital imaging, we study the role of adhesion molecules in regulating blood cell-endothelial (platelet and leukocyte) interaction followed by stimulation/ injury in vivo. In addition, research projects uses molecular and cellular techniques, combined with genetically modified mice to investigate the role of novel molecules in the pathophysiology of acute ischemic stroke and atherosclerosis. Using murine models, we hope to understand the pathophysiology of human vascular inflammatory diseases that may lead to development of novel therapies, thus improving human health. Current research project includes: 1) Role of ADAMTS13-VWF axis in acute ischemic stroke and atherosclerosis. 2) Mechanistic role of fibronectin alternative splicing in early and advanced atherosclerosis.

Publications

Prakash, P., Nayak, M. K. & Chauhan, A. K. (2016). P-selectin can promote thrombus propagation independently of both von Willebrand factor and thrombospondin1 in mice. Journal of thrombosis and haemostasis: JTH. PMID: 27943541.

Gu, S. X., Blokhin, I. O., Wilson, K. M., Dhanesha, N., Doddapattar, P., Grumbach, I. M., Chauhan, A. K. & Lentz, S. R. (2016). Protein methionine oxidation augments reperfusion injury in acute ischemic stroke. JCI insight, 1(7). PMID: 27294204.

Niki, M., Nayak, M. K., Jin, H., Bhasin, N., Plow, E. F., Pandolfi, P. P., Rothman, P. B., Chauhan, A. K. & Lentz, S. R. (2016). Dok-1 negatively regulates platelet integrin aIIbß3 outside-in signalling and inhibits thrombosis in mice. Thrombosis and haemostasis, 115(5), 969-78. PMID: 26790499.

Dhanesha, N., Prakash, P., Doddapattar, P., Khanna, I., Pollpeter, M. J., Nayak, M. K., Staber, J. M. & Chauhan, A. K. (2016). Endothelial Cell-Derived von Willebrand Factor Is the Major Determinant That Mediates von Willebrand Factor-Dependent Acute Ischemic Stroke by Promoting Postischemic Thrombo-Inflammation. Arteriosclerosis, thrombosis, and vascular biology, 36(9), 1829-37. PMID: 27444201.

Chauhan, A. (2016). MyNCBI bibliography.

Klutho, P. J., Pennington, S. M., Scott, J. A., Wilson, K. M., Gu, S. X., Doddapattar, P., Xie, L., Venema, A. N., Zhu, L. J., Chauhan, A. K., Lentz, S. R. & Grumbach, I. M. (2015). Deletion of Methionine Sulfoxide Reductase A Does Not Affect Atherothrombosis but Promotes Neointimal Hyperplasia and Extracellular Signal-Regulated Kinase 1/2 Signaling. Arteriosclerosis, Thrombosis, and Vascular Biology, 35(12), 2594–2604. PMID: 26449752.

Prakash, P., Kulkarni, P. P. & Chauhan, A. K. (2015). Thrombospondin 1 requires von Willebrand factor to modulate arterial thrombosis in mice. Blood, 125(2), 399–406. PMID: 25343959.

Jin, H., Gebska, M. A., Blokhin, I. O., Wilson, K. M., Ketsawatsomkron, P., Chauhan, A. K., Keen, H. L., Sigmund, C. D. & Lentz, S. R. (2015). Endothelial PPAR-? protects against vascular thrombosis by downregulating P-selectin expression. Arteriosclerosis, Thrombosis, and Vascular Biology, 35(4), 838–844. PMID: 25675995.

Prakash, P., Kulkarni, P. P., Lentz, S. R. & Chauhan, A. K. (2015). Cellular fibronectin containing extra domain A promotes arterial thrombosis in mice through platelet Toll-like receptor 4. Blood, 125(20), 3164–3172. PMID: 25700433.

Doddapattar, P., Gandhi, C., Prakash, P., Dhanesha, N., Grumbach, I. M., Dailey, M. E., Lentz, S. R. & Chauhan, A. K. (2015). Fibronectin Splicing Variants Containing Extra Domain A Promote Atherosclerosis in Mice Through Toll-Like Receptor 4. Arteriosclerosis, Thrombosis, and Vascular Biology, 35(11), 2391–2400. PMID: 26427793.

Dhanesha, N., Ahmad, A., Prakash, P., Doddapattar, P., Lentz, S. R. & Chauhan, A. K. (2015). Genetic Ablation of Extra Domain A of Fibronectin in Hypercholesterolemic Mice Improves Stroke Outcome by Reducing Thrombo-Inflammation. Circulation, 132(23), 2237-47. PMID: 26508731.

Gandhi, C., Ahmad, A., Wilson, K. M. & Chauhan, A. K. (2014). ADAMTS13 modulates atherosclerotic plaque progression in mice via a VWF-dependent mechanism. Journal of thrombosis and haemostasis: JTH, 12(2), 255–260. PMID: 24261607.

Chauhan, A. K. (2014). Degradation of platelet-von Willebrand factor complexes by plasmin: an alternative/backup mechanism to ADAMTS13. Circulation, 129(12), 1273–1275. PMID: 24449820.

Barr, J. D., Chauhan, A. K., Schaeffer, G. V., Hansen, J. K. & Motto, D. G. (2013). Red blood cells mediate the onset of thrombosis in the ferric chloride murine model. Blood, 121(18), 3733–3741. PMID: 23343833.

Barr, J. D., Chauhan, A. K., Schaeffer, G. V., Hensen, J. K. & Motto, D. G. (2013). Red blood cells mediate the onset of thrombosis in ferric-chloride murine model. Blood, 121(18), 3733-41. PMID: 23343833.

Dayal, S., Wilson, K., Motto, D. G., Miller, F. J., Chauhan, A. K. & Lentz, S. (2013). Hydrogen peroxide promotes aging-related platelet hyperactivation and thrombosis. Circulation, 127(12), 1308-16. PMID: 23426106.

Khan, M. M., Motto, D. G., Lentz, S. & Chauhan, A. K. (2012). ADAMTS13 reduces VWF-mediated acute inflammation following focal cerebral ischemia in mice. Journal of Thrombosis and Haemostasis, 10(8), 1665-71. PMID: 22712744.

Khan, M. M., Gandhi, C., Chauhan, N., Stevens, J. W., Motto, D. G., Lentz, S. & Chauhan, A. K. (2012). Alternatively-spliced extra domain A of fibronectin promotes acute inflammation and brain injury after cerebral ischemia in mice. Stroke, 43(5), 1376-82. PMID: 22363055.

Dayal, S., Chauhan, A. K., Jensen, M., Leo, L., Lynch, C. M., Faraci, F. M., Kruger, W. D. & Lentz, S. (2012). Paradoxical absence of a prothrombotic phenotype in a mouse model of severe hyperhomocysteinemia. Blood, 119(13), 3176-83. PMID: 22186991.

Gandhi, C., Motto, D. G., Jensen, M., Lentz, S. & Chauhan, A. K. (2012). ADAMTS13 deficiency exacerbates VWF-dependent acute myocardial ischemia/reperfusion injury in mice. Blood, 120(26), 5224-30. PMID: 22983446.

Gandhi, C., Khan, M. M., Lentz, S. & Chauhan, A. K. (2012). ADAMTS13 reduces vascular inflammation and the development of early atherosclerosis in mice. Blood, 119(10), 2385-91. PMID: 22123843.

Chauhan, A. K. (2011). A role of erythrocytes in a murine model of thrombosis. D. G. Motto , J. Barr , A. K. Chauhan (Eds.) ATVB.

Chauhan, A. K. (2011). Regulation of vascular thrombosis and P-selectin expression by endothelial PPARy. H. Jin , M. A. Gebska , A. K. Chauhan , H. L. Keen , C. D. Sigmund , S. R. Lentz (Eds.) ATVB.

Brill, A., Fuchs, T. A., Chauhan, A. K., Yang, J. J., DeMeyer, S. F., Kollenberger, M., Wakefield, T. W., Lammle, B., Massberg, S. & Wagner, D. D. (2011). von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models. Blood, 117(4), 1400-7. PMID: 20959603.

Flick, M. J., Chauhan, A. K., Frederick, M., Talmage, K. E., Kombrinck, K. W., Miler, W., Mullins, E. S., Palumbo, J. S., Zheng, X., Esmon, N. L., Esmon, C. T., Thornton, S., Becker, A., Pelc, l. A., Cera, E. D., Wagner, D. D. & Degen, J. L. (2011). The development of inflammatory joint disease is attenuated in mice expressing the anticoagulant prothrombin mutant W215A/E217A. Blood, 117(23), 6326-37. PMID: 21436072.

Chauhan, A. K. (2011). Extra domain A of fibronectin aggravates ischemia/reperfusion brain injury by enhancing thrombosis and inflammation. A. K. Chauhan , M. M. Khan , N. Gandhi , N. Chauhan , D. G. Motto , L. Dr (Eds.) ATVB.

Chauhan, A. K. (2010). EDA containing FN aggravates ischemic brain injury in mice. A. K. Chaukan , M. M. Khan , N. Gandhi , N. Chauhan , D. G. Motto , S. R. Lentz (Eds.) Blood.

Banno, F., Chauhan, A. K. & Miyata, T. (2010). The function of ADAMTS13 in thrombogenesis in vivo: insights from mutant mice. International Journal of Hematology, 91(1), 30-5. PMID: 20047094.

Chauhan, A. K. (2009). Prolonged survival following microbial infection in mice expressing the thrombin anticoagulant mutant W215/E217A. M. J. Flick , A. K. Chauhan , E. S. Mullins , J. S. Palumbo , S. Thornton , X. Zheng , N. L. Esmon , C. T. Esmon , D. D. Wagner , J. L. Degen (Eds.) (Vols. 7). (S2), pp. OC-TH-063. Journal of Thrombosis and Hemostasis.

Kisucka, J., Chauhan, A. K., Zhao, B., Patten, I. S., Yesilaltay, A., Krieger, M. & Wagner, D. D. (2009). Elevated levels of soluble P-selectin in mice alter blood-brain barrier function, exacerbate stroke, and promote atherosclerosis. Blood, 113(23), 6015-22. PMID: 19349621.

Brill, A., Chauhan, A. K., Cannault, M., Walsh, M. T., Bergmeier, W. & Wagner, D. D. (2009). Oxidative stress activates ADAM17/TACE and induces its target receptor shedding in platelets in a p38-dependent fashion. Cardiovascular Research, 84(1), 137-44. PMID: 19482949.

Zhao, B., Chauhan, A. K., Cannault, M., Patten, I. S., Yang, J. J., Dockal, M., Scheiflinger, F. & Wagner, D. D. (2009). von Willebrand factor-cleaving protease ADAMTS13 reduces ischemic brain injury in experimental stroke. Blood, 114(15), 3329-34. PMID: 19687510.

Banno, F., Chauhan, A. K., Kokame, K., Yang, J., Miyata, S., Wagner, D. D. & Miyata, T. (2009). The distal carboxyl-terminal domains of ADAMTS13 are required for regulation of in vivo thrombus formation. Blood, 113(21), 5323-9. PMID: 19109562.

Chauhan, A. K. (2009). . Image analysis of thrombus formation in ADAMTS13-mutant mice. F. Banno , A. K. Chauhan , K. Kokame , J. Yang , S. Miyata , D. D. Wagner (Eds.) (Vols. 7). (S2), pp. PP-MO-093. Journal of Thrombosis and Hemostasis.

Chauhan, A. K. (2009). Oxidative stress activates ADAM17 (TACE) in platelets and induces GPIb-alpha sheding in a p38 kinase-dependent fashion. A. Brill , A. K. Chauhan , M. Canault , M. T. Walsh , W. Bergmeier , D. D. Wagner (Eds.) (Vols. 7). (S2), pp. OC-TU-045. Journal of Thrombosis and Hemostasis.

Chauhan, A. K. (2009). Recombinant ADAMTS13 improves neurological outcome in experimental stroke in mice. A. K. Chauhan , B. Q. Zhao , M. Canault , I. S. Patten , J. J. Yang , M. Dockal , F. Scheiflinger , D. D. Wagner (Eds.) Blood.

Chauhan, A. K. (2008). VWF-cleaving protease ADAMTS13 reduces brain injury following ischemic stroke in mice: Essential role for VWF in stroke. B. Q. Zhao , A. K. Chauhan , I. S. Patten , M. Dockal , F. Scheiflinger , D. D. Wagner (Eds.) (Vols. 112). (11), pp. 259. Blood.

Bergmeier, W., Chauhan, A. K. & Wagner, D. D. (2008). Glycoprotein Ibalpha and von Willebrand factor in primary platelet adhesion and thrombus formation: lessons from mutant mice. Thrombosis and Haemostasis, 99(2), 264–270. PMID: 18278173.

Chauhan, A. K. (2008). factor in primary platelet adhesion and thrombus formation: lessons from mutant mice. Thromb & Haemost, 99(2), 264-70.

Chauhan, A. K., Walsh, M. T., Zhu, G., Ginsburg, D., Wagner, D. D. & Motto, D. G. (2008). The combined roles of ADAMTS13 and VWF in murine models of TTP, endotoxemia, and thrombosis. Blood, 111(7), 3452–3457. PMID: 18083848.

Lu, F., Chauhan, A. K., Fernandes, S. M., Walsh, M. T., Wagner, D. D. & Davis, A. E. (2008). The effect of C1 inhibitor on intestinal ischemia and reperfusion injury. American Journal of Physiology. Gastrointestinal and Liver Physiology, 295(5), G1042–1049. PMID: 18787060.

Chauhan, A. K., Kisucka, J., Brill, A., Walsh, M. T., Scheiflinger, F. & Wagner, D. D. (2008). ADAMTS13: a new link between thrombosis and inflammation. The Journal of Experimental Medicine, 205(9), 2065–2074. PMID: 18695007.

Kisucka, J., Chauhan, A. K., Patten, I. S., Yesilaltay, A., Neumann, C., Van Etten, R. A., Krieger, M. & Wagner, D. D. (2008). Peroxiredoxin1 prevents excessive endothelial activation and early atherosclerosis. Circulation Research, 103(6), 598–605. PMID: 18689572.

Chauhan, A. K. (2007). Peroxiredoxin, PrdX1 plays an important role in inflammation and early atherosclerosis. J. Kisucka , A. K. Chauhan , I. S. Patten , C. Neumann , R. A. Van Etten , D. D. Wagner (Eds.) (Vols. 5). (S2) Journal of Thrombosis and Hemostasis.

Chauhan, A. K. (2007). ten Cate H. Characterization of the role of platelet adhesion molecules in a murine model of photosensitizer-induced stroke. K. Frederix , A. K. Chauhan , J. Kisucka , S. HMH. , D. D. Wagner (Eds.) (Vols. 5). (S2), pp. P-W-455. Journal of Thrombosis and Hemostasis.

Chauhan, A. K. (2007). Prothrombotic effects of fibronectin isoforms containing the EDA domain., A. K. Chauhan , J. Kisucka , M. T. Walsh , F. A. Moretti , F. E. Baralle , A. F. Muro , D. D. Wagner (Eds.) (Vols. 5). (S2), pp. P-M-265. Journal of Thrombosis and Hemostasis.

Chauhan, A. K. (2007). Deficiency of the VWF-cleaving protease ADAMTs13 result sin increased leukocyte rolling and adhesion in mice. A. K. Chauhan , J. Kisucka , A. Brill , M. T. Walsh , D. D. Wagner (Eds.) (Vols. 110). pp. 290. Blood.

Chauhan, A. K., Goerge, T., Schneider, S. W. & Wagner, D. D. (2007). Formation of platelet strings and microthrombi in the presence of ADAMTS-13 inhibitor does not require P-selectin or beta3 integrin. Journal of thrombosis and haemostasis: JTH, 5(3), 583–589. PMID: 17166247.

Moretti, F. A., Chauhan, A. K., Iaconcig, A., Porro, F., Baralle, F. E. & Muro, A. F. (2007). A major fraction of fibronectin present in the extracellular matrix of tissues is plasma-derived. The Journal of Biological Chemistry, 282(38), 28057–28062. PMID: 17644525.

Chauhan, A. K. (2007). Absence of ADAMTS13 on a VWF-deficient background is no longer prethrombotic in a murine model of arterial thrombosis. A. K. Chauhan , M. T. Walsh , D. G. Motto , D. Ginsburg , D. D. Wagner (Eds.) (Vols. 5). (S2), pp. P-S-278. Journal of Thombosis and Hemostasis.

Frederix, K., Chauhan, A. K., Kisucka, J., Zhao, B., Hoff, E. I., Spronk, H. M., Ten Cate, H. & Wagner, D. D. (2007). Platelet adhesion receptors do not modulate infarct volume after a photochemically induced stroke in mice. Brain Research, 1185, 239–245. PMID: 17996853.

Chauhan, A. K. (2007). . Mice engineered to express a form of thrombin favoring protein C are resistant to S. aureus-induces sepsis. M. J. Flick , A. K. Chauhan , S. Welch , M. A. Shaw , K. E. Talmage , E. S. Mullins , J. S. Palumbo , S. Thornton , D. D. Wagner , J. L. Degen (Eds.) (Vols. 110). pp. 267. Blood.

Chauhan, A. K. (2006). Pro-thrombotic effects of EDA containing isoform of fibronectin. A. K. Chauhan , J. Kisucka , F. A. Moretti , M. Walsh , F. E. Baralle , A. F. Muro , D. D. Wagner (Eds.) Gordon Research Conference on Fibronectin, Integrins and Related Molecules,” Il Ciocco, Lucca, Italy.

Chauhan, A. K. (2006). von Willebrand Factor and Factor VIII are independently required to form stable occlusive thrombi in injured veins. A. K. Chauhan , J. Kisucka , C. B. Lamb , W. Bergmeier , D. D. Wagner (Eds.) (Vols. 108). (11), pp. 1789. Blood.

Kamath, A. F., Chauhan, A. K., Kisucka, J., Dole, V. S., Loscalzo, J., Handy, D. E. & Wagner, D. D. (2006). Elevated levels of homocysteine compromise blood-brain barrier integrity in mice. Blood, 107(2), 591–593. PMID: 16189268.

Yang, D., Zhang, Y., Nguyen, H. G., Koupenova, M., Chauhan, A. K., Makitalo, M., Jones, M. R., St Hilaire, C., Seldin, D. C., Toselli, P., Lamperti, E., Schreiber, B. M., Gavras, H., Wagner, D. D. & Ravid, K. (2006). The A2B adenosine receptor protects against inflammation and excessive vascular adhesion. The Journal of Clinical Investigation, 116(7), 1913–1923. PMID: 16823489.

Matuskova, J., Chauhan, A. K., Cambien, B., Astrof, S., Dole, V. S., Piffath, C. L., Hynes, R. O. & Wagner, D. D. (2006). Decreased plasma fibronectin leads to delayed thrombus growth in injured arterioles. Arteriosclerosis, Thrombosis, and Vascular Biology, 26(6), 1391–1396. PMID: 16528004.

Chauhan, A. K. (2006). Formation of platelet strings and microthrombi in the presence of ADAMTS13 inhibitor does not require P-selectin or ß3 integrin. A. K. Chauhan , D. D. Wagner (Eds.) (Vols. 108). (11), pp. 1782. Blood.

Chauhan, A. K., Motto, D. G., Lamb, C. B., Bergmeier, W., Dockal, M., Plaimauer, B., Scheiflinger, F., Ginsburg, D. & Wagner, D. D. (2006). Systemic antithrombotic effects of ADAMTS13. The Journal of Experimental Medicine, 203(3), 767–776. PMID: 16533881.

Chauhan, A. K. (2005). The metalloprotease ADAMTS13 is a natural anti-thrombotic. A. K. Chauhan , D. G. Motto , C. B. Lamb , M. Dockal , F. Scheiflinger , D. Ginsburg , D. D. Wagner (Eds.) (Vols. 106). (11), pp. 409. Blood.

Chauhan, A. K. (2005). Shigatoxin triggers Thrombotic Thrombocytopenic Purpura in genetically susceptible ADAMTS13-deficient mice. D. G. Motto , A. K. Chauhan , G. Zhu , J. Homeister , C. B. Lamb , K. C. Desch , H. M. Tsai , D. D. Wagner , D. Ginsburg (Eds.) (Vols. 106). pp. 214a. Blood.

Chauhan, A. K. (2005). Elevated levels of homocysteine compromise blood-brain barrier integrity in mice. A. K. Chauhan , A. K. Kamath , J. Kisucka , V. S. Dole , J. Loscalzo , D. E. Handy , D. D. Wagner (Eds.) (Vols. 106). (11), pp. 3856. Blood.

Chauhan, A. K., Moretti, F. A., Iaconcig, A., Baralle, F. E. & Muro, A. F. (2005). Impaired motor coordination in mice lacking the EDA exon of the fibronectin gene. Behavioural Brain Research, 161(1), 31–38. PMID: 15904707.

Chauhan, A. K. (2005). . Formation of platelet strings and microthrombi in the presence of ADAMTS13 inhibitor does not require P-selectin or ß3. A. K. Chauhan , D. D. Wagner (Eds.) integrinsGordon Research Conference on Hemostasis 2005, Waterville, ME.

Chauhan, A. K., Iaconcig, A., Baralle, F. E. & Muro, A. F. (2004). Alternative splicing of fibronectin: a mouse model demonstrates the identity of in vitro and in vivo systems and the processing autonomy of regulated exons in adult mice. Gene, 324, 55–63. PMID: 14693371.

Muro, A. F., Chauhan, A. K., Gajovic, S., Iaconcig, A., Porro, F., Stanta, G. & Baralle, F. E. (2003). Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan. The Journal of Cell Biology, 162(1), 149–160. PMID: 12847088.

Chauhan, A. K. (2003). Sustained platelet adhesion and VWF string froomation in stimulated venules of Adamts13-/- mice. A. K. Chauhan , D. G. Motto , D. Lamb , D. Ginsburg , D. D. Wagner (Eds.) (Vols. 3). (S1) Journal of Thrombosis and Hemostasis.

Chauhan, A. K. (2002). Regulated splicing of the fibronectin EDA exon (FN) is essential for proper skin wound healing and normal life span. A. K. Chauhan , A. Muro , S. Gajovic , A. Iaconcig , F. Porro , G. Stanta , F. E. Baralle (Eds.) (Vols. 8). (supp S) Mol. Biol. Cell.