Madhu Singh, PhD
Research Assistant Professor of Internal Medicine-Endocrinology and Metabolism
Current Positions
- Research Assistant Professor of Internal Medicine-Endocrinology and Metabolism
Education
- PhD, University of Alberta, Edmonton, Canada
- Postdoctoral Fellowship, Division of Dermatology, University of Alberta, Edmonton, Alberta, Canada
Center, Program and Institute Affiliations
Research Interests
- My research goal is to understand how inflammation caused by the innate immune system regulates hypertension and associated vascular tissue damage. The innate immune system detects pathogens and cellular injury through molecular receptors, such as toll-like receptors (TLRs), and initiates several signaling pathways in response. We have found that a branch of the TLR pathways also regulates hypertension in animal models. Mice with mutation in a specific adaptor protein (TRIF) of the TLR do not develop hypertension upon angiotensin II treatment, whereas deletion of another major adaptor protein MyD88 results in enhanced inflammatory gene expression and hypertension. Thus, the MyD88 pathways may attenuate the TRIF-dependent hypertension. Our current research efforts are directed to determine the effective organ and tissues where these events take place. To this end, we are using an integrated approach of whole animal physiology, genetically engineered animals, immunological methods including bone marrow transfer, and molecular biological approaches of gene expression and mass spectrometry.
Selected Publications
- Mani AM, Dhanabalan K, Lamin V, Wong T, Singh MV, Dokun AO. BAG3 Attenuates Ischemia-Induced Skeletal Muscle Necroptosis in Diabetic Experimental Peripheral Artery Disease. Intl J Mol Sci 2022 Sep 14;23(18):10715. PMCID: PMC9502689
- Singh MV, Dhanabalan K, Verry J, Dokun AO. MicroRNA regulation of BAG3. Exp Biol Med 2022 Apr;247(8):617-23. PMCID: PMC9039493
- Abboud FM, Cicha MZ, Ericsson A, Chapleau MW, Singh MV. Altering early life gut microbiota has long-term effect on immune system and hypertension in spontaneously hypertensive rats. Front Physiol 2021 Oct;12:752924. PMCID: PMC8586697
- Alleboina S, Wong T, Singh MV, Dokun AO. Inhibition of protein kinase C beta phosphorylation activates nuclear factor kappa B and improves postischemic recovery in type1 diabetes. Exp Biol Med 2020 May;245(9):785-96. PMCID: PMC7273893
- Singh MV, Cicha MZ, Nunez S, Chapleau MW, Abboud FM. Angiotensin II-induced hypertension and cardiac hypertrophy are differentially mediated by TLR3- and TLR4-dependent pathways. Am J Physiol Heart Circ Physiol 2019 May;316(5):H1027-38. PMCID: PMC6580398
- Wang R, Lu Y, Cicha MZ, Singh MV, Benson CJ, Madden CJ, Chapleau MW, Abboud FM. TMEM16B determines cholecystokinin sensitivity of intestinal vagal afferents of nodose neurons. JCI Insight 2019 Mar;4(5):e122058. PMCID: PMC6483506
- Singh, M. V., Cicha, M. Z., Kumar, S., Meyerholz, D. K., Irani, K., Chapleau, M. W. & Abboud, F. M. (2017). Abnormal CD161+ immune cells and retinoic acid receptor–related orphan receptor γt–mediate enhanced IL-17F expression in the setting of genetic hypertension. Journal of Allergy and Clinical Immunology 140 (3) 809-821.e3. DOI: 10.1016/j.jaci.2016.11.039.
- Abboud, F. M. & Singh, M. V. (2017). Autonomic regulation of the immune system in cardiovascular diseases. Advances in Physiology Education 41 (4) 578-593. DOI: 10.1152/ADVAN.00061.2017.
- Singh, M. V. (2016). Toll-Like Receptors, Hypertension, and an Antimalarial Drug. American Journal of Hypertension. DOI: 10.1093/ajh/hpw128. PMID: 27702749.
- Singh, M. V., Cicha, M. Z., Meyerholz, D. K., Chapleau, M. W. & Abboud, F. M. (2015). Dual Activation of TRIF and MyD88 Adaptor Proteins by Angiotensin II Evokes Opposing Effects on Pressure, Cardiac Hypertrophy, and Inflammatory Gene Expression. Hypertension 66 (3) 647-656. DOI: 10.1161/HYPERTENSIONAHA.115.06011.