Mark A. Stamnes, PhD

Portrait
Associate Professor of Molecular Physiology and Biophysics
Associate Professor of Internal Medicine

Contact Information

Primary Office: 6-550 BSB
51 Newton Road
Iowa City, IA 52242
319-335-7858

Lab: 6-555 BSB
51 Newton Road
Iowa City, IA 52242
319-335-7859

Education

BS, Cellular and Molecular Biology, University of Washington
PhD, Biology, University of California, San Diego

Fellow, Memorial Sloan-Kettering Cancer Center

Education/Training Program Affiliations

Department of Molecular Physiology and Biophysics PhD, Interdisciplinary Graduate Program in Molecular and Cellular Biology

Research Summary

A central requirement for eukaryotic cell growth and function is the ability to transport and sort proteins. Defects in protein trafficking can lead to debilitating diseases such as cystic fibrosis and Alzheimer's disease. Much of protein transport in a cell occurs via coated transport vesicles. My laboratory's research interests focus on the molecular events involved in the formation of transport vesicles, the selection of cargo molecules into vesicles, and the cellular regulation of protein transport. We use the mammalian Golgi apparatus as a model system for these studies. Transport vesicles can be generated from Golgi membranes in a cell-free system, allowing a biochemical dissection of this process. Projects in the lab include the identification and characterization of novel proteins and phospholipids that play roles in cargo selection of vesicle formation, and an analysis of the role of the cytoskeleton in vesicular transport. A longer term goal of the laboratory is to understand how, once cellular components are properly transported and sorted, they can be assembled into complex cellular structures such as the axons and dendrites of neurons and the microvilli of epithelial cells.

Publications

MacDonald, C., Stamnes, M. A., Katzmann, D. J. & Piper, R. C. (2015). Tetraspan cargo adaptors usher GPI-anchored proteins into multivesicular bodies. Cell cycle (Georgetown, Tex.), 14(23), 3673-8. PMID: 26505929.

Schultz, M. L., Tecedor, L., Stein, C. S., Stamnes, M. A. & Davidson, B. L. (2014). CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events. PloS one, 9(5), e96647. PMID: 24792215.

Chi, X., Wang, S., Huang, Y., Stamnes, M. & Chen, J. L. (2013). Roles of rho GTPases in intracellular transport and cellular transformation. International journal of molecular sciences, 14(4), 7089-108. PMID: 23538840.

Stamnes, M. A. (2013). Golgi Complex. Elsevier, Oxford,(2).

Peterson, T., Stamnes, M. A. (2011). Analyzing the role of a Golgi transport-vesicle coat protein in PKC-epsilon-dependent signaling. San Diego, CA: ASCB Annual meeting.

Stroter, R., Stamnes, M. A. (2010). Determining if Coatomer Protein Dependent Localization of Cdc42 and PKCe Contribute to Human Cancers. Charlottte, NC: Annual Biomedical Research Conference for Minority Students (ABRCMS).

Stamnes, M., Xu, W. (2010). The reconstitution of actin polymerization on liposomes. Methods in molecular biology (Clifton, N.J.), 606, 95-103. PMID: 20013392.

Stamnes, M. A., Xu, W. (2010). The use of liposomes for studying regulated cytoskeletal dynamics at the membrane. Methods Mol. Biol., 606, 95-103.

Hehnly, H., Xu, W., Chen, J. & Stamnes, M. A. (2010). Cdc42 regulates microtubule-dependent Golgi positioning. (Vols. 11). pp. 1067-1078. Traffic.

Hehnly, H., Longhini, K., Chen, J. L. & Stamnes, M. A. (2009). Retrograde Shiga toxin trafficking is regulated by ARHGAP21 and Cdc42. (Vols. 20). pp. 4303-4312. Mol. Biol. Cell.

Hehnly, H. A., Stamnes, M. A. (2008). Rho GTPases regulat the dynein-dependent motility of Shiga toxin tothe juxtanuclear Golgi apparatus. Pavia, Italy: The Golgi Meeting: Membrane trafficking in global cellular responses.

Hehnly, H., Stamnes, M. A. (2007). Regulating cytoskeleton-based vesicle motility. FEBS lett., 581, 2112-2118.

Hehnly, H. A., Stamnes, M. A. (2006). Shiga Toxin: A Model for Studying the Regulation of Dynein based Motility. San Diego, CA: ASCB Annual meeting.

Xu, W. D., Stamnes, M. A. (2006). The ADFH and charged/helical domains of drebrin and mAbp1 direct membrane binding and localization via distinct interactions with actin. (Vols. 281). pp. 11826-11833. J. Biol. Chem..

Xu, W., Stamnes, M. (2006). The actin-depolymerizing factor homology and charged/helical domains of drebrin and mAbp1 direct membrane binding and localization via distinct interactions with actin. The Journal of biological chemistry, 281(17), 11826-33. PMID: 16452483.

Xu, W., Stamnes, M. A. (2006). Identification of effector proteins that mediate signaling between Golgi vesicles and the cytoskeleton. San Diego, CA: ASCB Annual meeting.

Hehnly, H., Sheff, D. & Stamnes, M. A. (2006). Shiga toxin facilitates its retrograde transport by modifying microtubule dynamics. (Vols. 17). pp. 4379-4389. Mol. Biol. Cell.

Pelish, H. E., Peterson, J. R., Salvarezza, S. B., Rodriguez-Boulan, E., Chen, J., Stamnes, M. A., Macia, E., Feng, Y., Shair, M. D. & Kirchhausen, T. (2006). Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. (Vols. 2). pp. 39-46. Nat. Chem. Biol..

Cao, H., Weller, S., Orth, J., Chen, J., Huang, B., Chen, J. L., Stamnes, M. A. & McNiven, M. A. (2005). The actin-binding protein cortactin recruits dynamin to the trans-Golgi network and is required for post-Golgi transport. (Vols. 7). pp. 483-492. Nat. Cell. Biol..

Chen, J., Xu, W. & Stamnes, M. A. (2005). In vitro reconstitution of ARF-regulated cytoskeletal dynamics on Golgi membranes. Methods Enzymol, 404, 345-358.

Cao, H., Weller, S., Orth, J. D., Chen, J., Huang, B., Chen, J. L., Stamnes, M. & McNiven, M. A. (2005). Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport. Nature cell biology, 7(5), 483-92. PMID: 15821732.

Chen, J. L., Fucini, R. V., Lacomis, L., Erdjument-Bromage, H., Tempst, P. & Stamnes, M. A. (2005). Coatomer-bound Cdc42 regulates dynein recruitment to COPI vesicles. (Vols. 169). pp. 383-389. J. Cell Biol..

Chen, J. J., Fucini, R. V., Erdjument-Bromage, H., Tempst, P. & Stamnes, M. A. (2004). The regulated recruitment of dynein to COPI-coated vesicles. Breckenridge, CO: Keystone Symposium on Golgi apparatus and secretory pathway of eukaryotic cells.

Chen, J. L., Lacomis, L., Erdjument-Bromage, H., Tempst, P. & Stamnes, M. A. (2004). Cytosol-derived proteins are sufficient for Arp2/3 recruitment and ARF/coatomer-dependent actin polymerization on Golgi membranes. (Vols. 566). pp. 281-286. FEBS lett..

Cao, H., Weller, S., Orth, J., Chen, J. J., Huang, B., Chen, J. J., Stamnes, M. A. & McNiven, M. A. (2004). Secretory vesicle formation from the TGN is mediated by the F-actin, dynamin-binding protein cortactin.

Stamnes, M. A. (2004). Golgi Complex. Elsevier, Oxford, 2, 312-315.

Chen, J. J., Stamnes, M. A. (2003). The regulation of vesicular transport and cytoskeletal dynamics at the Golgi apparatus via a coatomer/Cdc42 complex. In ASCB Annual Meeting. (Vols. 14). pp. 106a. Mol. Biol. Cell.

Xu, W., Stamnes, S. & Stamnes, M. A. (2003). Characterization of protein motifs important for the localization of the ARF-1 sensitive actin-binding proteins mAbp1 and drebrin. In ASCB Annual meeting. (Vols. 14). pp. 106a. Mol. Biol. Cell.

Chen, J. J., Fucini, R. V., Erdjument-Bromage, H., Tempst, P. & Stamnes, M. A. (2002). A Regulatory Link between Actin Dynamics and Dynein Recruitment to the Golgi. In ASCB Annual meeting. pp. 13. Mol. Biol. Cell.

Ahluwalia, J. P., O'Neill, M. S. & Stamnes, M. A. (2002). Cell-free Characterization of Calcium-Sensitive Component(s) Affecting COPI Vesicle Assembly. In ASCB Annual meeting. (Vols. 13). pp. 267A. Mol. Biol. Cell.

Ahluwalia, J. P., O'Neill, M. & Stamnes, M. A. (2002). The role of calcium in transport vesicle coat assembly: identification of calcium-sensitive components. In The Liver Meeting. (Vols. 36). pp. 239A. Hepatology.

Kanzaki, M., Watson, R. T., Stamnes, M. A., Saltiel, A. R. & Pessin, J. E. (2002). The small GTP binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes. (Vols. 13). pp. 2334-2346. Mol. Biol. Cell.

Cao, H., Weller, S., Orth, J., Chen, J. J., Huang, B., Chen, J. J., Stamnes, M. A. & McNiven, M. A. (2002). The F-actin, Dynamin, Binding Protein Cortactin Associates with the Trans-Golgi Network to Mediate Secretory Vesicle Formation. In ASCB Annual meeting. (Vols. 13). Mol. Biol. Cell.

Chen, J. L., Ahluwalia, J. & Stamnes, M. A. (2002). Selective effects of calcium chelators on anterograde and retrograde protein transport in the cell. (Vols. 277). pp. 35682-356847. J. Biol. Chem..

Fucini, R. V., Chen, J. J., Sharma, C., Kessels, M. & Stamnes, M. A. (2002). Golgi vesicle proteins are linked to the assembly of an actin complex defined by mAbp1. (Vols. 13). pp. 621-631. Mol. Biol. Cell.

Stamnes, M. A. (2002). Regulating the actin cytoskeleton during vesicular transport. Curr. Opin. Cell Biol, 14, 428-433.

Fucini, R. V., Sharma, C. & Stamnes, M. A. (2001). Regulation of the actin cytoskeleton during transport vesicle assembly. Airlie, VA: Keith R. Symposium on Cytoplasmic Organization and Membrane Traffic.

Ahluwalia, J., Topp, J., Weirather, K., Zimmerman, M. & Stamnes, M. A. (2001). A role for calcium in maintaining the integrity of vesicle coats. (Vols. 276). pp. 34148-34155. J. Biol. Chem..

Fucini, R. V., Sharma, C. & Stamnes, M. A. (2000). Regulation of the actin cytoskeleton during vesicle formation on the Golgi. In ASCB Annual meeting. (Vols. 11 (Supplement)). pp. 280a. Mol. Biol. Cell.

Fucini, R. V., Sharma, C. & Stamnes, M. A. (2000). Regulation of the actin cytoskeleton during vesicle formation on the Golgi. Madison, WI: Steenbock Symposium on Intracellular Protein and Lipid Traffic.

Fucini, R. V., Navarrete, A., Vadakkan, C., Lacomis, L., Erdjument-Bromage, H., Tempst, P. & Stamnes, M. A. (2000). Activated ADP-ribosylation factor assembles distinct pools of actin on Golgi membrans. (Vols. 275). pp. 18824-18829. J. Biol. Chem..

Ahluwalia, J., Topp, J., Weirather, K., Zimmerman, M. & Stamnes, M. A. (2000). A role for calcium in regulating coat stability on the Golgi. In ASCB Annual meeting. (Vols. 11 (Supplement)). pp. 278a. Mol. Biol. Cell.

Stamnes, M. A., Vadakkan, C., Navarrete, A., Topp, J. & Fucini, R. V. (1999). ARF regulation of Golgi-Bound cytoskeletal proteins. In ASCB Annual Meeting. (Vols. 10 (Supplement)). pp. 116a. Mol. Biol. Cell.

Stamnes, M. A., Schiavo, G., Stenbeck, G., Sollner, T. H. & Rothman, J. E. (1998). ADP-ribosylation factor and phosphatidic acid levels in Golgie membranes during budding of coatomer-coated vesicles. (Vols. 95). pp. 13676-13680. Proc. Natl. Acad. Sci. USA.

Orci, L., Stamnes, M. A., Ravazzola, M., Amherdt, M., Perrelet, A., Sollner, T. H. & Rothman, J. E. (1997). Bidirectional transport by distinct populations of COPI-coated vesicles. (Vols. 90). pp. 335-349. Cell.

Fiedler, K., Veit, M., Stamnes, M. A. & Rothman, J. E. (1996). Bimodal interaction of coatomer with a family of putative cargo and vesicle coat protein receptors, the p24 proteins. (Vols. 273). pp. 1396-1399. Science.

Stamnes, M. A., Craighead, M. W., Hoe, M. H., Lampen, N., Geromanos, S., Tempst, P. & Rothman, J. E. (1995). An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding. (Vols. 92). pp. 8011-8015. Proc. Natl. Acad. Sci. USA.

Videen, J. S., Stamnes, M. A., Hsu, V. L. & Goodman, M. (1994). Thermodynamics of cyclophilin catalyzed peptidyl-prolyl isomerization by NMR spectroscopy. (Vols. 34). pp. 171-175. Biopolymers.

Stamnes, M. A., Rothman, J. E. (1993). The binding of AP-1 clathrin adaptor particles to Golgie membranes requires ADP-ribosylation factor, a small GTP-binding protein. (Vols. 73). pp. 999-1005. Cell.

Stamnes, M. A., Rutherford, S. L. & Zuker, C. S. (1992). Cyclophilins: a new family of proteins involved in intracellular folding. Trends in Cell Biology, 2, 272-276.

Ondek, B., Hardy, R., Baker, E., Stamnes, M. A., Shieh, B. & Zuker, C. S. (1992). Genetic dissectiono f cyclophilin function: saturation mutagenesis of the Drosophila cyclophilin homolog ninA. (Vols. 267). pp. 16460-16466. J. Biol. Chem.

Colley, N. J., Baker, E. K., Stamnes, M. A. & Zuker, C. S. (1991). The cyclophilin homolog, ninaA, is required in the secretory pathway. (Vols. 67). pp. 255-263. Cell.

Stamnes, M. A., Shieh, B., Chuman, L., Harris, G. L. & Zuker, C. S. (1991). The cyclophilin homolog ninaA is a tissue-specific integral membrane protein required for the proper synthesis of a subset of Drosophila rhodosins. (Vols. 65). pp. 219-227. Cell.

Smith, D. P., Stamnes, M. A. & Zuker, C. S. (1991). Signal transduction in the visual system of Drosophilia. Ann. Rev. Cell Biol, 7, 161-190.

Stamnes, M. A., Zuker, C. S. (1990). Peptidyl-prolylcis-transisomerases, cyclophilin, FK506 binding protein, and ninaA: four of a kind. Curr. Opin. Cell Biol., 2, 1104-1107.

Shieh, B., Stamnes, M. A., Seavello, S., Harris, G. L. & Zuker, C. S. (1989). ninaA, a gene required for visual transduction in Drosophila encodes a homolog of the cyclosporin A binding protein. (Vols. 338). pp. 67-70. Nature.

Hopkins, R. S., Stamnes, M. A., Simon, M. I. & Hurley, J. B. (1988). Cholera toxin and pertussin toxin substrates and endogenous ADP-ribosyltransferase activity in Drosophila melanogaster. (Vols. 970). pp. 355-362. Biochem. Biophys. Acta.

Voight, T., LeVay, S. & Stamnes, M. A. (1988). Morphological and immunocytochemical observations on the visual callosal projections in the cat. (Vols. 272). pp. 450-460. J. Comp. Neurol..