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Ryan L. Boudreau, PhD

Associate Professor of Internal Medicine-Cardiovascular Medicine

Current Positions

  • Associate Professor of Internal Medicine-Cardiovascular Medicine

Education

  • PhD, Molecular Physiology & Biophysics, University of Iowa
  • Postdoctoral Fellow, The University of Iowa, Iowa City, Iowa
  • PhD, Molecular Physiology & Biophysics, The University of Iowa, Iowa City, Iowa

Graduate Program Affiliations

Center, Program and Institute Affiliations

Research Interests

  • My laboratory studies post-transcriptional responses in diseased and developing heart and brain using viral vectors, mouse models, human tissues, and cell culture systems. Our goal is to identify disease-related RNA-binding proteins (RBPs) and microRNAs (miRNAs) and determine their regulatory targets using computational and high-throughput biochemical means (HITS-CLIP, CLIP-seq). RBPs and miRNAs are key mediators of post-transcriptional responses, and modulation of their activities provides attractive means to manipulate gene expression for therapeutic benefit.
  • Another major aspect of my laboratory's research involves determining how human genetic variations contribute to disease by altering post-transcriptional regulatory mechanisms. This work aims to generate valuable resources (e.g. miRNA target interactomes in human tissues) to accelerate our understanding of miRNA and RBP functions in heart and brain and facilitate the translation of available and ongoing genetic studies towards novel or refined disease mechanisms and therapies.
  • In addition to this, we are also investigating the functions of several novel genes, including long non-coding RNAs, in heart and brain. These projects incorporate a breadth of techniques, including viral-based (AAV) overexpression and inhibition (RNAi) of genes in vivo, generation and characterization of CRISPR-derived knockout mice, and gene transfer and functional assays in human iPS-derived cells.
  • Overall, my research program is balanced in basic and translational studies, wet-lab and computational methods, and resource- and hypothesis-driven research. This framework promotes multi-disciplinary and collaborative science, offering an excellent environment to foster the growth of current and future trainees.

Selected Publications

  • Wang, Y., Chen, B., Huang, C. K., Guo, A., Wu, J., Zhang, X., Chen, R., Chen, C., Kutschke, W., Weiss, R. M., Boudreau, R. L., Margulies, K. B., Hong, J. & Song, L. S. (2018). Targeting Calpain for Heart Failure Therapy: Implications From Multiple Murine Models. JACC. Basic to translational science 3 (4) 503-517. PMCID: PMC6115647.
  • Matkovich, S. J. & Boudreau, R. L. (2018). When Knowing "Enough" May Still Not Be Enough. Circulation research 123 (4) 412-414. PMID: 30355252.
  • Stein, C. S., Jadiya, P., Zhang, X., McLendon, J. M., Abouassaly, G. M., Witmer, N. H., Anderson, E. J., Elrod, J. W. & Boudreau, R. L. (2018). Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency. Cell reports 23 (13) 3710-3720.e8. PMCID: PMC6091870.
  • Zhang, X., Yoon, J. Y., Morley, M., McLendon, J. M., Mapuskar, K. A., Gutmann, R., Mehdi, H., Bloom, H. L., Dudley, S. C., Ellinor, P. T., Shalaby, A. A., Weiss, R., Tang WHW, Moravec, C. S., Singh, M., Taylor, A. L., Yancy, C. W., Feldman, A. M., McNamara, D. M., Irani, K., Spitz, D. R., Breheny, P., Margulies, K. B., London, B. & Boudreau, R. L. (2018). A common variant alters SCN5A-miR-24 interaction and associates with heart failure mortality. The Journal of clinical investigation 128 (3) 1154-1163. PMCID: PMC5824852.
  • Boudreau, R. L. (2017). The Next Best Thing in Cardiovascular Research: Highlights From the Basic Cardiovascular Sciences Scientific Sessions. Journal of the American Heart Association 6 (9). PMCID: PMC5634317.
  • Liu, W., Deng, J., Ding, W., Wang, G., Shen, Y., Zheng, J., Zhang, X., Luo, Y., Lv, C., Wang, Y., Chen, L., Yan, D., Boudreau, R. L., Song, L. S. & Liu, J. (2017). Decreased KCNE2 Expression Participates in the Development of Cardiac Hypertrophy by Regulation of Calcineurin-NFAT (Nuclear Factor of Activated T Cells) and Mitogen-Activated Protein Kinase Pathways. Circulation. Heart failure 10 (6). PMID: 28611128.
  • Vikram, A., Lewarchik, C. M., Yoon, J. Y., Naqvi, A., Kumar, S., Morgan, G. M., Jacobs, J. S., Li, Q., Kim, Y. R., Kassan, M., Liu, J., Gabani, M., Kumar, A., Mehdi, H., Zhu, X., Guan, X., Kutschke, W., Zhang, X., Boudreau, R. L., Dai, S., Matasic, D. S., Jung, S. B., Margulies, K. B., Kumar, V., Bachschmid, M. M., London, B. & Irani, K. (2017). Sirtuin 1 regulates cardiac electrical activity by deacetylating the cardiac sodium channel. Nature medicine 23 (3) 361-367. PMCID: PMC6218171.
  • Kim, Y. C., Miller, A., Lins, L. C., Han, S. W., Keiser, M. S., Boudreau, R. L., Davidson, B. L. & Narayanan, N. S. (2017). RNA Interference of Human α-Synuclein in Mouse. Frontiers in neurology 8 13. PMCID: PMC5281542.
  • Spengler, R. M., Zhang, X., Cheng, C., McLendon, J. M., Skeie, J. M., Johnson, F. L., Davidson, B. L. & Boudreau, R. L. (2016). Elucidation of transcriptome-wide microRNA binding sites in human cardiac tissues by Ago2 HITS-CLIP. Nucleic acids research 44 (15) 7120-31. PMID: 27418678.
  • Rasmussen, T. P., Wu, Y., Joiner, M. L., Koval, O. M., Wilson, N. R., Luczak, E. D., Wang, Q., Chen, B., Gao, Z., Zhu, Z., Wagner, B. A., Soto, J., McCormick, M. L., Kutschke, W., Weiss, R. M., Yu, L., Boudreau, R. L., Abel, E. D., Zhan, F., Spitz, D. R., Buettner, G. R., Song, L. S., Zingman, L. V. & Anderson, M. E. (2015). Inhibition of MCU forces extramitochondrial adaptations governing physiological and pathological stress responses in heart. Proceedings of the National Academy of Sciences of the United States of America 112 (29) 9129-34. PMID: 26153425.