Logo for University of Iowa Health Care This logo represents the University of Iowa Health Care

Brandon Schickling


brandon.schickling@duke.edu
Mentor: Francis Miller, M.D.
Lab Room: 2257 CBRB
Lab Phone: 319-335-9521

Novel Molecular Mechanisms of Vascular Smooth Muscle Cell Dedifferentiation Contributing to the Pathogenesis of Vascular Disease

Cardiovascular disease (CVD) is the leading cause of mortality in the United States. Neointimal formation is a fundamental process contributing to vascular diseases including atherosclerosis, restenosis and pulmonary arterial hypertension (PAH). Vascular smooth muscle cell (SMC) dedifferentiation contributes to the development of neointimal formation through increased migration and proliferation of SMCs into the subendothelial space. This SMC phenotypic modulation is inherently complex and dependent on coordinated changes in signal transduction, gene expression and chromatin modifications in response to inflammation or injury.

Our research focuses on the molecular mechanisms that contribute to vascular SMC dedifferentiation and vascular disease development. We are using integrated approaches encompassing high-throughput assays and informatics to determine the spatiotemporal causes of vascular disease.

Thiel WH, Esposito CL, Dickey DD, Dassie JP, Long ME, Adam J, Streeter J, Schickling B, Takapoo M, Flenker KS, Klesney-Tait J, Franciscis Vd, Miller FJ Jr, Giangrande PH. Smooth Muscle Cell-targeted RNA Aptamer Inhibits Neointimal Formation. Molecular therapy : the journal of the American Society of Gene Therapy. 2016; 24(4):779-87. PubMed [journal] PMID: 26732878, PMCID: PMC4886937

Devor EJ, Schickling BM, Reyes HD, Warrier A, Lindsay B, Goodheart MJ, Santillan DA, Leslie KK. Cullin-5, a ubiquitin ligase scaffold protein, is significantly underexpressed in endometrial adenocarcinomas and is a target of miR-182. Oncology reports. 2016; 35(4):2461-5. PubMed [journal] PMID: 26847831, PMCID:PMC4774736

A novel redox-dependent epigenetic dedifferentiation of vascular smooth muscle cells Schickling,Brandon,M, Takapoo,Maysam,, Devor,Eric,J, Miller,Francis,J. Department of Internal Medicine Research Day; 2016 March; Iowa City, IA, United States. c2016. My Bibliography [meeting]

Gimenez M, Schickling BM, Lopes LR, Miller FJ Jr. Nox1 in cardiovascular diseases: regulation and pathophysiology. Clinical science (London, England :1979). 2016; 130(3):151-65. PubMed [journal] PMID: 26678171

Modulation of Smooth Muscle Cell Differentiation. Schickling,Brandon,M, Miller,Francis,J. United States 62/257,594. 2015 November. My Bibliography [patent]

Coordinated MicroRNA Silencing of Nox4 NADPH Oxidase and Myocardin Regulates Smooth Muscle Cell Dedifferentiation in Vascular Disease Schickling,Brandon,M, Miller,Francis,J. Society for Free Radical Biology & Medicine 22nd Annual Conference; 2015 November; Boston, MA, USA. Elsevier; c2015. My Bibliography [meeting]

Redox-Dependent Interaction Between Protein Disulfide Isomerase and P47phox activates Nox1 NADPH Oxidase in Vascular Disease Gimenez,Marcela,, Schickling,Brandon,M, Wittig,I,, Miller,Francis,J, Lopes,Lucia,R. Society for Free Radical Biology & Medicine 22nd Annual Conference; 2015 November; Boston, MA, USA. Elsevier; c2015. My Bibliography [meeting]

Nox4-dependent epigenetic regulation of the dedifferentiation of vascular smooth muscle cells. Schickling,Brandon,M, Miller,Francis,J. Molecular and Cellular Biology Retreat 2015; 2015 October; West Branch, IA, USA. MCB Program; c2015. My Bibliography [meeting]

Redox dependent interaction between protein disulfide isomerase and NADPH oxidase regulatory subunit p47phox increases Nox1 complex assembly and superoxide generation contributing to oxidative stress and vascular disease Lopes,Lucia,R, Gimenez,Marcela,, Schickling,Brandon,M, Miller,Francis,J. 23rd Congress of the International Union for Biochemistry and Molecular Biology, 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology; 2015 August; Foz do Iguaçu, PR, Brazil. c2015. My Bibliography [meeting]

Mir-9 mediates Nox4-dependent regulation of myocardin in human smooth muscle cells. Schickling,Brandon,M, Miller,Francis,J. Oberley Symposium 2015; 2015 June 05; Iowa City, IA, USA. c2015. My Bibliography [meeting]



Honors and Awards

  • American Heart Association Predoctoral Grant 07/2016 to 06/2018