Logo for University of Iowa Health Care This logo represents the University of Iowa Health Care

Rebecca Marquez


rmarquez2@kumc.edu
Mentor: Anton McCaffrey, Ph.D.
Lab Phone: 335-7870

The Role of MicroRNAs in Gene Expression

MicroRNAs (miRNAs) were recently discovered as key regulators of gene expression. miRNAs are thought to regulate about one third of all human genes by binding to the 3’ untranslated regions (UTRs)of target mRNAs . miRNAs have been shown to be involved in development, tissue differentiation, cell proliferation, apoptosis, oncogenesis and viral infection. We are interested in determining whether miRNAs are involved in viral pathogenesis.

Bandyopadhyay S, Friedman RC, Marquez RT, Keck K, Kong B, Icardi MS, Brown KE, Burge CB, Schmidt WN, Wang Y, McCaffrey AP. Hepatitis C virus infection and hepatic stellate cell activation downregulate miR-29: miR-29 overexpression reduces hepatitis C viral abundance in culture. J Infect Dis. 2011 Jun 15;203(12):1753-62. PubMed PMID: 21606534; PubMed Central PMCID: PMC3143452.

Marquez RT, Bandyopadhyay S, Wendlandt EB, Keck K, Hoffer BA, Icardi MS, Christensen RN, Schmidt WN, and McCaffrey AP., Correlation between microRNA expression levels and clinical parameters associated with chronic hepatitis C viral infection in humans, Lab Invest., PMID: 20625373 [Epub ahead of print], 2010.

Marquez RT, Wendlandt E, Galle CS, Keck K, and McCaffrey AP., MicroRNA-21 is upregulated during the proliferative phase of liver regeneration, targets Pellino-1, and inhibits NF-kappaB signaling, Am J Physiol Gastrointest Liver Physiol. 298(4):G535-41, PMID: 20167875, 2010.

Feng W, Marquez RT, Lu Z, Liu J, Lu KH, Issa JP, Fishman DM, Yu Y, and Bast RC Jr., Imprinted tumor suppressor genes ARHI and PEG3 are the most frequently down-regulated in human ovarian cancers by loss of heterozygosity and promoter methylation, Cancer, 112(7):1489-502, PMID: 18286529, 2008.

Marquez RT and McCaffrey AP, Advances in microRNAs: implications for gene therapists, Hum Gene Ther., 19(1):27-38. Review, PMID: 18092919, 2008.

Marquez RT, Baggerly KA, Patterson AP, Liu J, Broaddus R, Frumovitz M, Atkinson EN, Smith DI, Hartmann L, Fishman D, Berchuck A, Whitaker R, Gershenson DM, Mills GB, Bast RC Jr, and Lu KH., Patterns of gene expression in different histotypes of epithelial ovarian cancer correlate with those in normal fallopian tube, endometrium, and colon. Clin Cancer Res., 11(17):6116-26, PMID: 16144910, 2005.

Lu KH, Patterson AP, Wang L, Marquez RT, Atkinson EN, Baggerly KA, Ramoth LR, Rosen DG, Liu J, Hellstrom I, Smith D, Hartmann L, Fishman D, Berchuck A, Schmandt R, Whitaker R, Gershenson DM, Mills GB, and Bast RC Jr., Selection of potential markers for epithelial ovarian cancer with gene expression arrays and recursive descent partition analysis. Clin Cancer Res., 10(10):3291-300, PMID: 15161682, 2004.

Liu J, Yang G, Thompson-Lanza JA, Glassman A, Hayes K, Patterson A, Marquez RT, Auersperg N, Yu Y, Hahn WC, Mills GB, and Bast RC Jr., A genetically defined model for human ovarian cancer. Cancer Res., 64(5):1655-63, PMID: 14996724, 2004.

Liu Y, Ali SM, Boge TC, Georg GI, Victory S, Zygmunt J, Marquez RT, and Himes RH., A systematic SAR study of C10 modified paclitaxel analogues using a combinatorial approach. Comb Chem High Throughput Screen. 5(1):39-48, PMID: 11860338, 2002.

Gupta ML Jr, Bode CJ, Dougherty CA, Marquez RT, and Himes RH., Mutagenesis of beta-tubulin cysteine residues in Saccharomyces cerevisiae: mutation of cysteine 354 results in cold-stable microtubules. Cell Motil Cytoskeleton., 49(2):67-77, PMID: 11443737, 2001.



Honors and Awards

  • 2006 Molecular & Cellular Biology Retreat - $250 travel award for best poster.