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Roman Rodionov


roman-rodionov@uiowa.edu
Mentor: Steven Lentz, M.D./Ph.D.
Lab Phone: 335-8857

Role of asymmetric dimethyl arginine in endothelial dysfunction

My career goal is to be a physician-researcher, spending some time with patients and some time in the lab. My area of clinical interest is cardiology. My area of research is vascular biology. The major focus of research in Dr. Lentz's laboratory is endothelial function in vascular diseases and tissue injury, with particular emphasis on structure, function, and regulation of hemostatic proteins. Currently I'm working on elucidating of the role of homocysteine in endothelial dysfunction. A major mechanism for endothelial dysfunction is impaired bioavailability of nitric oxide (NO). We hypothesize that decreased NO bioavailability is mediated partly by asymmetric dimethyl arginine (ADMA). ADMA is an endogenous inhibitor of endothelial nitric oxide synthase (eNOS). Elevated plasma levels of ADMA have been found in association with several risk factors for cardiovascular disease, including hypercholesterolemia, hypertension and diabetes mellitus. In preliminary work we have demonstrated that plasma levels of ADMA are elevated in hyperhomocysteinemia. Currently we are using genetic approaches in mice and adenoviral vectors to determine mechanisms of ADMA level elevation in cardiovascular diseases.

Lentz SR, Rodionov RN, Dayal S. Hyperhomocysteinemia, endothelial dysfunction, and cardiovascular risk: the potential role of ADMA. Atheroscler Suppl. 2003;4:61-65.

Dayou H Rodionov RN, Lynch C, Cooke JP, Arning E, Bottiglieri T, Lentz SR, Faraci FM. Overexpression of dimethylarginine dimethylaminohydrolase inhibits asymmetric dimethylarginine-induced endothelial dysfunction in the cerebral circulation. Stroke. 2008 Jan;39(1):180-4.

Rodionv, RN, Lentz SR. The Homocysteine Paradox. Arterioscler Thromb Vasc Biol. 2008 Jun;28(6):1031-3.

Dayl S, Rodionov RN, Arning E, Bottiglieri T, Kimoto M, Murry DJ, Cooke JP, Faraci FM, Lentz SR. Tissue-specific downregulation of dimethylarginine dimethylaminohydrolase in hyperhomocysteinemia. Am J Physiol Heart Circ Physiol. 2008 Aug;295(2):H816-25.

Rodionv RN, Lentz SR. Many potential explanations for the homocysteine paradox. Arterioscler Thromb Vasc Biol. 2008 Oct; 28(10): e161.

Rodionv RN, Lentz S.R. Hyperhomocysteinemia as a cardiovascular risk factor. Current concepts. Arterialnaya Gipertoniya 2008;14(2):110-115 (article in Russian).

Rodionv RN, Blokhin IO, Galagudza MM, Shlyakhto EV, Lentz SR. Asymmetric dimethylarginine and its role in pathogenesis of cardiovascular diseases. Arterialnaya Gipertoniya 2008;14(4):306-314. (article in Russian).

Rodionov RN, Dayoub H, Lynch C, Wilson KM, Murry DJ, Kimoto M, Arning E, Bottiglieri T, Cooke JP, Faraci FM, Lentz SR. Role of ADMA in Mediating Vascular Dysfunction in Hyperhomocysteinemia: Effects of Overexpression of Dimethylarginine Dimethylaminohydrolase. Circ Res. 2010 Feb 19;106(3):551-8.

Rodionv RN, Murry DJ, Lentz SR. Human alanine:glyoxylate aminotransferase 2 protects from ADMA-mediated impairment in nitric oxide production. J Biol Chem. 2010 Feb 19;285(8):5385-91.

Abstracts:

Dayou H, Rodionov R, Cooke JP, Lentz SR, Faraci FM. Human DDAH-1 transgenic mice exhibit increased basal vascular NO and are protected against ADMA-induced endothelial dysfunction. FASEB J. (2005) 19:A1238

Dayoub H, Lynch C, Rodionov R, Cooke JP, Lentz SR, Faraci FM. Overexpression of dimethylarginine dimethylaminohydrolase (DDAH-1) in mice inhibits effects of ADMA on endothelial function in the cerebral circulation. FASEB J. (2006) 20: Abstract #466.4

Rodioov RN , Dayoub H, Wilson KM, Cooke JP, Faraci FM, Lentz SR Transgenic overexpression of DDAH1 protects from ADMA-mediated endothelial dysfunction but not from hyperhomocysteinemia-induced endothelial dysfunction or thrombosis. The 3rd International Meeting on ADMA, London (2006), oral presentation

Dayal,S Rodionov RN, Arning E, Bottiglieri T, Kimoto M, Cooke JP, Lentz SR. Tissue-specific downregulation of DDAH expression in hyperhomocysteinemic mice. The 3rd International Meeting on ADMA, London (2006)

Dayoub H, Lynch C, Rodionov RN, Cooke JP, Lentz SR, Faraci FM. Overexpression of dimethylarginine dimethylaminohydrolase (DDAH-1) in mice inhibits effects of ADMA on endothelial function in the cerebral circulation. The 3rd International Meeting on ADMA, London (2006)

Rodionv RN, Dayoub H, Lynch C, Wilson KM, Kimoto M, Arning E, Bottiglieri T, Cooke JP, Faraci FM, Lentz SR. Overexpression of Dimethylarginine Dimethylaminohydrolase Lowers ADMA and Protects from Vascular Dysfunction in Hyperhomocysteinemic Mice. ASCI/AAP Joint Meeting, Chicago (2007)

Rodionv RN, Dayoub H, Lynch C, Wilson KM, Kimoto M, Arning E, Bottiglieri T, Cooke JP, Faraci FM, Lentz SR. Overexpression of dimethylarginine dimethylaminohydrolase lowers ADMA and protects from vascular dysfunction in hyperhomocysteinemic mice. Clin. Chem. Lab. Med. (2007) 45:A38, oral presentation

Rodionv RN, Murry DJ, Lentz SR. Ectopic Overexpression of Human Alanine:glyoxylate aminotransferase 2 Lowers ADMA and Protects from ADMA-induced Impairment in Nitric Oxide Production. 4th International Symposium on ADMA, Bregenz (2008), oral presentation

Rodioov RN, Dayoub H, Arning E, Bottiglieri T, Cooke JP, Baumbach GL, Faraci FM, Lentz SR. Overexpression of Dimethylarginine Dimethylaminohydrolase Protects Against Vascular Dysfunction and Decreases Medial Thickness in Cerebral Arterioles of Hyperhomocysteinemic Mice. 4th International Symposium on ADMA, Bregenz (2008) oral presentation.

Rodionv RN, Murry DJ, Lentz SR. Human Alanine:glyoxylate Aminotransferase 2 Lowers ADMA and Protects from ADMA-induced Impairment in Nitric Oxide Production. American Heart Association Scientific Sessions, Orlando (2009), oral presentation. Circulation. 2009;120:S1134.

Honors and Awards

  • 2010 Schwarz-Monheim Award from German Society of Vascular Medicine for the manuscript Rodionov R.N. et al. Circ Res. 2010 Feb 19;106(3):551-8. (10,000 €)
  • 2008 1st prize for best oral presentation, 4th International Conference on ADMA, Bregenz, Austria (300 €)
  • 2005-2007 American Heart Association Predoctoral Fellowship Award, “Role of Asymmetric Dimethylarginine in the Endothelial Dysfunction of Hyperhomocysteinemia” ($50,000 over two years)
  • 2006 Travel Award, 3rd International Meeting on ADMA, London, UK
  • 2006 Travel Award for best presentation, Molecular & Cellular Biology Retreat, University of Iowa
  • 2003 Travel Award for best poster, Molecular Biology Retreat, University of Iowa