Ute Müh

Assistant Research Scientist, Ellermeier LaboratoryUte Müh
Address: 200 EMRB
Phone: (319) 335-7622
Email: ute-muh@uiowa.edu

Education/Training

BS in Biology, Technical University of Munich, Germany
MS in Biology, University of Konstanz, Germany
PhD in Biological Chemistry, University of Michigan
Postdoctoral Fellow, Microbiology, Phillipps University Marburg
Postdoctoral Fellow, Chemistry, MIT

Research

After 17 years of antibacterial discovery research in the pharmaceutical industry, I am now studying the other side of the battle: how do bacteria respond to stress such as antibacterial assault? Bacteria of interest in the Ellermeier lab are Bacillus subtilis, Bacillus thuringiensis and Clostridium difficile. Stressors include beta-lactam antibiotics as well as lysozyme, an important component of the host innate immune response.

Publications

1. Müh, U., V. Massey, and C.H. Williams, Jr., Lactate monooxygenase. I. Expression of the mycobacterial gene in Escherichia coli and site-directed mutagenesis of lysine 266. J Biol Chem., 1994. 269(11): p. 7982-8.

2. Müh, U., C.H. Williams, Jr., and V. Massey, Lactate monooxygenase. III. Additive contributions of active site residues to catalytic efficiency and stabilization of an anionic transition state. J Biol Chem., 1994. 269(11): p. 7994-8000.

3. Müh, U., C.H. Williams, Jr., and V. Massey, Lactate monooxygenase. II. Site-directed mutagenesis of the postulated active site base histidine 290. J Biol Chem., 1994. 269(11): p. 7989-93.

4. Müh, U., et al., 4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction. Biochemistry., 1996. 35(36): p. 11710-8.

5. Müh, U., W. Buckel, and E. Bill, Mössbauer study of 4-hydroxybutyryl-CoA dehydratase--probing the role of an iron-sulfur cluster in an overall non-redox reaction. Eur J Biochem., 1997. 248(2): p. 380-4.

6. Müh, U., et al., PHA synthase from Chromatium vinosum: cysteine 149 is involved in covalent catalysis. Biochemistry, 1999. 38(2): p. 826-37.

7. Yuan, W., et al., Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies. Arch Biochem Biophys., 2001. 394(1): p. 87-98.

8. Mani, N., et al., In vitro characterization of the antibacterial spectrum of novel bacterial type II topoisomerase inhibitors of the aminobenzimidazole class. Antimicrob Agents Chemother., 2006. 50(4): p. 1228-37.

9. Müh, U., et al., A structurally unrelated mimic of a Pseudomonas aeruginosa acylhomoserine lactone quorum-sensing signal. Proc Natl Acad Sci U S A., 2006. 103(45): p. 16948-52. Epub 2006 Oct 30.

10. Müh, U., et al., Novel Pseudomonas aeruginosa quorum-sensing inhibitors identified in an ultra-high-throughput screen. Antimicrob Agents Chemother., 2006. 50(11): p. 3674-9. Epub 2006 Sep 11.

11. Sarrazin, C., et al., Dynamic hepatitis C virus genotypic and phenotypic changes in patients treated with the protease inhibitor telaprevir. Gastroenterology., 2007. 132(5): p.1767-77. Epub 2007 Feb 21.

12. Zhou, Y., et al., Phenotypic and structural analyses of hepatitis C virus NS3 protease Arg155 variants: sensitivity to telaprevir (VX-950) and interferon alpha. J Biol Chem., 2007. 282(31): p. 22619-28. Epub 2007 Jun 6.

13. Zhou, Y., et al., Phenotypic Characterization of Resistant Val36 Variants of Hepatitis C Virus NS3-4A Serine Protease. Antimicrob Agents Chemother., 2008. 52(1): p. 110-20.Epub 2007 Oct 15.

14. Jiang, M., et al., In vitro phenotypic characterization of hepatitis C virus NS3 protease variants observed in clinical studies of telaprevir. Antimicrob Agents Chemother., 2013. 57(12): p. 6236-45. Epub 2013 Oct 7.