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Dominique H. Limoli, PhD

Assistant Professor of Microbiology and Immunology

Contact Information

Primary Office
203 Eckstein Medical Research Building (EMRB)
431 Newton Rd
Iowa City, IA 52242

200J Eckstein Medical Research Building (EMRB)
431 Newton Rd
Iowa City, IA 52242


BS, Biological Sciences, Ohio University
PhD, Biomedical Research, Emphasis in Microbial Pathogenesis, Ohio State University
Postdoctoral Fellow, Emory University
Postdoctoral Fellow, Geisel School of Medicine at Dartmouth

Education/Training Program Affiliations

Department of Microbiology Graduate Program, Medical Scientist Training Program

Research Summary

Our research focuses on understanding interactions between microbial species during airway infection in individuals with cystic fibrosis. Data from our lab and others reveal interspecies interactions can alter microbial metabolism, motility, antibiotic tolerance, and production of virulence factors. We seek to understand the cellular mechanisms driving these interactions in order to modulate polymicrobial community behavior and improve patient outcomes. We utilize live-cell imaging, bacterial genetics, and molecular biology approaches to interrogate polymicrobial interactions at the population and single-cell level, in both in vitro models of infection and in ex vivo patient samples.


Fischer, A. J., Singh, S. B., LaMarche, M. M., Maakestad, L. J., Kienenberger, Z. E., Peña, T. A., Stoltz, D. A. & Limoli, D. H. (2021). Sustained Coinfections with Staphylococcus aureus and Pseudomonas aeruginosa in Cystic Fibrosis. American journal of respiratory and critical care medicine, 203(3), 328-338. PMID: 32750253.

Yarrington, K. D., Sánchez Peña, A. & Limoli, D. H. (2020). Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions. Journal of visualized experiments : JoVE,(162). PMID: 32831301.

Price, C. E., Brown, D. G., Limoli, D. H., Phelan, V. V. & O'Toole, G. A. (2020). Exogenous alginate protects Staphylococcus aureus from killing by Pseudomonas aeruginosa. Journal of bacteriology, 202(8), e00559-19. PMID: 31792010.

Limoli, D. H. (2020). mSphere of Influence: A community to study communities. mSphere, 5(1), e00047-20. PMID: 32024707.

Fischer, A. J., Kilgore, S. H., Singh, S. B., Allen, P. D., Hansen, A. R., Limoli, D. H. & Schlievert, P. M. (2019). High prevalence of Staphylococcus aureus enterotoxin gene cluster superantigens in cystic fibrosis clinical isolates. Genes (Basel), 10(12), E1036. PMID: 31842331.

Limoli, D. H., Warren, E. A., Yarrington, K. D., Donegan, N. P., Cheung, A. L. & O'Toole, G. A. (2019). Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa. eLife, 8, e47365. PMID: 31713513.

Limoli, D. H., Hoffman, L. R. (2019). Help, hinder, hide and harm: what can we learn from the interactions between Pseudomonas aeruginosa and Staphylococcus aureus during respiratory infections?. Thorax, 74(7), 684-692. PMID: 30777898.

Forrest, O. A., Ingersoll, S. A., Preininger, M. K., Laval, J., Limoli, D. H., Brown, M. R., Lee, F. E., Bedi, B., Sadikot, R. T., Goldberg, J. B., Tangpricha, V., Gaggar, A. & Tirouvanziam, R. (2018). Frontline Science: Pathological conditioning of human neutrophils recruited to the airway milieu in cystic fibrosis. Journal of leukocyte biology, 104(4), 665-675. PMID: 29741792.

Malhotra, S., Limoli, D. H., English, A. E., Parsek, M. R. & Wozniak, D. J. (2018). Mixed communities of mucoid and nonmucoid Pseudomonas aeruginosa exhibit enhanced resistance to host antimicrobials. mBio, 9(2), e00275-18. PMID: 29588399.

Limoli, D. H., Whitfield, G. B., Kitao, T., Ivey, M. L., Davis, Jr, M. R., Grahl, N., Hogan, D. A., Rahme, L. G., Howell, P. L., O'Toole, G. A. & Goldberg, J. B. (2017). Pseudomonas aeruginosa alginate overproduction promotes coexistence with Staphylococcus aureus in a model of cystic fibrosis respiratory infection. mBio, 8(2), e00186-17. PMID: 28325763.

Limoli, D. H., Yang, J., Khansaheb, M. K., Helfman, B., Peng, L., Stecenko, A. A. & Goldberg, J. B. (2016). Staphylococcus aureus and Pseudomonas aeruginosa co-infection is associated with cystic fibrosis-related diabetes and poor clinical outcomes. European journal of clinical microbiology & infectious diseases, 35(6), 947-53. PMID: 26993289.

Limoli, D. H., Jones, C. J. & Wozniak, D. J. (2015). Bacterial extracellular polysaccharides in biofilm formation and function. Microbiology spectrum, 3(3). PMID: 26185074.

Limoli, D. H., Wozniak, D. J. (2014). Mutagenesis by host antimicrobial peptides: insights into microbial evolution during chronic infections. Microbial cell, 1(7), 247-249. PMID: 28357249.

Limoli, D. H., Rockel, A. B., Host, K. M., Jha, A., Kopp, B. T., Hollis, T. & Wozniak, D. J. (2014). Cationic antimicrobial peptides promote microbial mutagenesis and pathoadaptation in chronic infections. PLoS pathogens, 10(4), e1004083. PMID: 24763694.

Jones, C. J., Newsom, D., Kelly, B., Irie, Y., Jennings, L. K., Xu, B., Limoli, D. H., Harrison, J. J., Parsek, M. R., White, P. & Wozniak, D. J. (2014). ChIP-Seq and RNA-Seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa. PLoS pathogens, 10(3), e1003984. PMID: 24603766.

Limoli, D. H., Sladek, J. A., Fuller, L. A., Singh, A. K. & King, S. J. (2011). BgaA acts as an adhesin to mediate attachment of some pneumococcal strains to human epithelial cells. Microbiology, 157(Pt 8), 2369-81. PMID: 21602213.

Marion, C., Limoli, D. H., Bobulsky, G. S., Abraham, J. L., Burnaugh, A. M. & King, S. J. (2009). Identification of a pneumococcal glycosidase that modifies O-linked glycans. Infection and immunity, 77(4), 1389-96. PMID: 19139197.