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Research letter by Isaac Jensen and Dr. Patrick McGonagill in Dr. Vladimir Badovinac’s laboratory is published in the journal "Immunity"

April 3, 2020

Isaac Jensen and Dr. Patrick McGonagill

The letter, entitled Worry and FRET: ROS Production Leads to Fluorochrome Tandem Degradation and Impairs Interpretation of Flow Cytometric Result, describes how intrinsic sample-to-sample differences in ROS production differentially impact fluorophophore tandem degradation leading to false positive detection of signal from the “parent” fluorophore of the tandem construct. This effect is particularly prevalent in samples obtained from highly inflammatory states (e.g., sepsis or infection) and can lead to erroneous interpretation of data when compared to samples from a substantially lower inflammatory state, such as normal naïve tissue.

This work represents a collaborative effort between the laboratories of Drs. Vladimir Badovinac, John Harty and Thomas Griffith (University of Minnesota).

The article by Jensen et al. is in the most recent issue of Immunity and can be viewed at:  https://www.cell.com/immunity/fulltext/S1074-7613(20)30074-1