Patrick Sinn, Ph.D.

Associate Research Professor
Research Associate Professor of Pediatrics - Pulmonary Medicine, Allergy and Immunology

Contact Information

6318 PBDB
169 Newton Road
Iowa City, IA 52242


BA, Biology, University of Minnesota
PhD, Physiology and Biophysics, University of Iowa
Postdoctoral Fellow, Program in Gene Therapy, University of Iowa

Research Summary

The cornerstone of Dr. Sinn's research is to develop integrating vector systems for the treatment of inherited diseases such as Cystic Fibrosis (CF). Current main areas of focus include: 1) maximizing transduction efficiency in epithelial cells of the airway; 2) assessing host immune responses to transduction by lentiviral vectors; and 3) creating hybrid viral/DNA transposon vectors. To improve the transduction efficiency of lentiviral vectors, he has performed extensive screening of viral envelopes to identify those compatible with the vector that confer improved delivery to the respiratory tract. In addition, he has identified vector formulations that increase the efficiency of vector delivery to the airways, such as viscoelastic gels. The group can obtain long-term gene expression in the respiratory tract of mice following a single application of the vector. Very little is known about the immunologic consequences of the administration of single or multiple doses of the lentiviral vector to the respiratory tract. Dr. Sinn is investigating the potential for enhancing gene expression through more than one application of the vector and evaluating host humoral and cellular responses. A potential problem with all integrating vector systems is the disruption of the function of an endogenous gene and perturbing host cell function. Ideally this could be avoided if the vector integration complex could be guided to a "safe site" in the genome. Dr. Sinn is also pursuing strategies to modify the integration properties of the lentiviral vector system to address this important vector safety issue.

Publications from PubMed[au]