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Yi Luo, MD, MS, PhD

Portrait
Professor of Urology

Contact Information

Office
240D Eckstein Medical Research Building (EMRB)
Iowa City, IA 52242
319-335-9835

Education

MD, Nanjing Medical University
MS, Immunobiochemistry, Nanjing Medical University
PhD, Molecular Biology, University of New Brunswick
Fellow, Roswell Park Cancer Institute, Molecular Immunology
Postdoctoral Fellow, Harvard Medical School, Urology

Research Summary

Dr Luo's research focuses on urinary bladder inflammation and cancer, specifically the interplay between the bladder and the immune system. This field is challenging due to a lack of proper animal models. In the early of the 2000s, Dr. Luo’s team used genetic engineering technology to develop a novel transgenic murine model that expresses the membrane form of the well-defined model antigen ovalbumin (OVA) as a self-antigen on the urothelium. This model can develop autoimmune-based bladder inflammation upon introduction of OVA-specific T and/or B cells. This model system facilitates the immune mechanistic studies pertaining to immune cell tolerance, activation, and autoimmune responses. Using this model system, Dr. Luo and his colleagues have demonstrated the ability of the bladder urothelium to present self-antigen to the immune system. This work has led to a panel of novel animal models with experimental autoimmune cystitis (EAC) that have been used in interstitial cystitis (IC) research over 15 years. The recent work of Dr. Luo’s team focuses on identification of the role of regulatory T cells in controlling autoimmune cystitis, characterization of the functional outcomes of the animal models, and development of new treatments for chronic cystitis pain. In addition, Dr. Luo's projects include the development of recombinant BCGs for bladder cancer immunotherapy, identification of biological pathways and genes responsible for bladder cancer resistance to chemotherapy, and establishment of new animal models for bladder cancer research.

Publications

Akiyama, Y., Yao, J. R., Kreder, K. J., O'Donnell, M. A., Lutgendorf, S. K., Lyu, D., Maeda, D., Kume, H., Homma, Y. & Luo, Y. (2020). Autoimmunity to urothelial antigen causes bladder inflammation, pelvic pain and voiding dysfunction: a novel animal model for Hunner type interstitial cystitis. American journal of physiology. Renal physiology, 320(2), F174-F182. PMID: 33308017.

Smith, N., Saunders, D., Lerner, M., Zalles, M., Mamedova, N., Cheong, D., Mohammadi, E., Yuan, T., Luo, Y., Hurst, R. E., Greenwood-Van Meerveld, B. & Towner, R. A. (2020). In vivo and ex vivo assessment of bladder hyper-permeability and using molecular targeted magnetic resonance imaging to detect claudin-2 in a mouse model for interstitial cystitis. PloS one, 15(10), e0239282. PMID: 33095778.

Akiyama, Y., Luo, Y., Hanno, P. M., Maeda, D. & Homma, Y. (2020). Interstitial cystitis/bladder pain syndrome: The evolving landscape, animal models and future perspectives. International journal of urology : official journal of the Japanese Urological Association, 27(6), 491-503. PMID: 32246572.

Cui, X., Jing, X., Lutgendorf, S. K., Bradley, C. S., Schrepf, A., Erickson, B. A., Magnotta, V. A., Ness, T. J., Kreder, K. J., O'Donnell, M. A. & Luo, Y. (2019). Cystitis-induced bladder pain is Toll-like receptor 4 dependent in a transgenic autoimmune cystitis murine model: a MAPP Research Network animal study. American journal of physiology. Renal physiology, 317(1), F90-F98. PMID: 31091120.

Woodworth, D. C., Dagher, A., Curatolo, A., Sachdev, M., Ashe-McNalley, C., Naliboff, B. D., Labus, J. S., Landis, J. R., Kutch, J. J., Mayer, E. A., Lee, R. S., Moses, M. A., Ellingson, B. M. & MAPP Research Network, (2018). Changes in brain white matter structure are associated with urine proteins in urologic chronic pelvic pain syndrome (UCPPS): A MAPP Network study. PloS one, 13(12), e0206807. PMID: 30517112.

Schrepf, A., Naliboff, B., Williams, D. A., Stephens-Shields, A. J., Landis, J. R., Gupta, A., Mayer, E., Rodriguez, L. V., Lai, H., Luo, Y., Bradley, C., Kreder, K. & Lutgendorf, S. K. (2018). Adverse Childhood Experiences and Symptoms of Urologic Chronic Pelvic Pain Syndrome: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Study. Annals of behavioral medicine : a publication of the Society of Behavioral Medicine, 52(10), 865-877. PMID: 30212850.

Kogan, P., Xu, S., Wang, Y., O'Donnell, M. A., Lutgendorf, S. K., Bradley, C. S., Schrepf, A., Kreder, K. J. & Luo, Y. (2018). Sub-noxious Intravesical Lipopolysaccharide Triggers Bladder Inflammation and Symptom Onset in A Transgenic Autoimmune Cystitis Model: A MAPP Network Animal Study. Scientific reports, 8(1), 6573. PMID: 29700406.

Sweeney, S. K., Luo, Y., O'Donnell, M. A. & Assouline, J. G. (2017). Peptide-Mediated Targeting Mesoporous Silica Nanoparticles: A Novel Tool for Fighting Bladder Cancer. Journal of biomedical nanotechnology, 13(2), 232-42. PMID: 29377654.

Wang, X., Liu, W., O'Donnell, M., Lutgendorf, S., Bradley, C., Schrepf, A., Liu, L., Kreder, K. & Luo, Y. (2016). Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PloS one, 11(12), e0168772. PMID: 28002455.

Stephens-Shields, A. J., Clemens, J. Q., Jemielita, T., Farrar, J., Sutcliffe, S., Hou, X., Landis, J. R. & MAPP Research Network, (2016). Symptom Variability and Early Symptom Regression in the MAPP Study: A Prospective Study of Urological Chronic Pelvic Pain Syndrome. The Journal of urology, 196(5), 1450-1455. PMID: 27131464.

Xu, S., Wang, X., Wang, Y., Lutgendorf, S., Bradley, C., Schrepf, A., Kreder, K., O'Donnell, M. & Luo, Y. (2016). Transgenic Mice Expressing MCP-1 by the Urothelium Demonstrate Bladder Hypersensitivity, Pelvic Pain and Voiding Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PloS one, 11(9), e0163829. PMID: 27684718.

Schrepf, A., O'Donnell, M. A., Luo, Y., Bradley, C. S., Kreder, K. J. & Lutgendorf, S. K. (2016). Inflammation and Symptom Change in Interstitial Cystitis or Bladder Pain Syndrome: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Study. Urology, 90, 56-61. PMID: 26768711.

Sweeney, S. K., Luo, Y., O'Donnell, M. A. & Assouline, J. (2016). Nanotechnology and cancer: improving real-time monitoring and staging of bladder cancer with multimodal mesoporous silica nanoparticles. Cancer nanotechnology, 7, 3. PMID: 27217840.

Schrepf, A., Bradley, C. S., O'Donnell, M., Luo, Y., Harte, S. E., Kreder, K. & Lutgendorf, S. (2015). Toll-like receptor 4 and comorbid pain in Interstitial Cystitis/Bladder Pain Syndrome: a multidisciplinary approach to the study of chronic pelvic pain research network study. Brain, behavior, and immunity, 49, 66-74. PMID: 25771510.

Lai, H., Gereau, 4th, R. W., Luo, Y., O'Donnell, M., Rudick, C. N., Pontari, M., Mullins, C. & Klumpp, D. J. (2015). Animal Models of Urologic Chronic Pelvic Pain Syndromes: Findings From the Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network. Urology, 85(6), 1454-65. PMID: 26099889.

Krieger, J. N., Stephens, A. J., Landis, J. R., Clemens, J. Q., Kreder, K., Lai, H. H., Afari, N., Rodríguez, L., Schaeffer, A., Mackey, S., Andriole, G. L., Williams, D. A. & MAPP Research Network, (2015). Relationship between chronic nonurological associated somatic syndromes and symptom severity in urological chronic pelvic pain syndromes: baseline evaluation of the MAPP study. The Journal of urology, 193(4), 1254-62. PMID: 25444992.

Schrepf, A., O'Donnell, M., Luo, Y., Bradley, C. S., Kreder, K. & Lutgendorf, S. (2014). Inflammation and inflammatory control in interstitial cystitis/bladder pain syndrome: Associations with painful symptoms. Pain, 155(9), 1755-61. PMID: 24907404.

Clemens, J. Q., Mullins, C., Kusek, J. W., Kirkali, Z., Mayer, E. A., Rodríguez, L. V., Klumpp, D. J., Schaeffer, A. J., Kreder, K. J., Buchwald, D., Andriole, G. L., Lucia, M. S., Landis, J. R., Clauw, D. J. & MAPP Research Network, (2014). The MAPP research network: a novel study of urologic chronic pelvic pain syndromes. (Vols. 14). pp. 57. BMC urology. PMID: 25085007.

Landis, J. R., Williams, D. A., Lucia, M. S., Clauw, D. J., Naliboff, B. D., Robinson, N. A., van Bokhoven, A., Sutcliffe, S., Schaeffer, A. J., Rodriguez, L. V., Mayer, E. A., Lai, H. H., Krieger, J. N., Kreder, K. J., Afari, N., Andriole, G. L., Bradley, C. S., Griffith, J. W., Klumpp, D. J., Hong, B. A., Lutgendorf, S. K., Buchwald, D., Yang, C. C., Mackey, S., Pontari, M. A., Hanno, P., Kusek, J. W., Mullins, C., Clemens, J. Q. & MAPP Research Network, (2014). The MAPP research network: design, patient characterization and operations. BMC urology, 14, 58. PMID: 25085119.

Newton, M. R., Askeland, E. J., Andresen, E. D., Chehval, V. A., Wang, X., Askeland, R. W., O'Donnell, M. A. & Luo, Y. (2014). Anti-interleukin-10R1 monoclonal antibody in combination with bacillus Calmette--Guérin is protective against bladder cancer metastasis in a murine orthotopic tumour model and demonstrates systemic specific anti-tumour immunity. Clinical and experimental immunology, 177(1), 261-8. PMID: 24593764.

Luo, Y., Askeland, E. J., Newton, M. R., Henning, J. R. & O’Donnell, M. A. (2013). Immunotherapy of Urinary Bladder Carcinoma: BCG and Beyond. In L. Rangel (Eds.) Cancer Treatment - Conventional and Innovative Approaches. pp. 319-361. InTech Open Access Publisher.

Luo, Y. (2012). Blocking IL-10 enhances bacillus Calmette-Guérin induced T helper Type 1 immune responses and anti-bladder cancer immunity. Oncoimmunology, 1(7), 1183-1185. PMID: 23170273.

Bockholt, N. A., Knudson, M. J., Henning, J. R., Maymí, J. L., Weady, P., Smith, 3rd, G. J., Eisenbraun, M. D., Fraser, J. D., O'Donnell, M. A. & Luo, Y. (2012). Anti-interleukin-10R1 monoclonal antibody enhances bacillus Calmette-Guérin induced T-helper type 1 immune responses and antitumor immunity in a mouse orthotopic model of bladder cancer. The Journal of urology, 187(6), 2228-35. PMID: 22503050.

Askeland, E. J., Newton, M. R., O'Donnell, M. A. & Luo, Y. (2012). Bladder Cancer Immunotherapy: BCG and Beyond. Advances in urology, 2012, 181987. PMID: 22778725.

Koc, G., Wang, X. & Luo, Y. (2011). Pazopanib: an orally administered multi-targeted tyrosine kinase inhibitor for locally advanced or metastatic renal cell carcinoma. The Canadian journal of urology, 18(6), 5991-7. PMID: 22166325.

Liu, W., Chen, X., Evanoff, D. P. & Luo, Y. (2011). Urothelial antigen-specific CD4+ T cells function as direct effector cells and induce bladder autoimmune inflammation independent of CD8+ T cells. Mucosal immunology, 4(4), 428-37. PMID: 21270773.

Luo, Y., Henning, J. & O'Donnell, M. (2011). Th1 cytokine-secreting recombinant bacillus Calmette-Guerin: Prospective use in immunotherapy of bladder cancer. In Ö. Özdemir (Eds.) Current Cancer Treatment \textendash Novel Beyond Conventional Approaches. pp. 63-90. InTech Open Access Publisher.

Kim, R., Liu, W., Chen, X., Kreder, K. J. & Luo, Y. (2011). Intravesical dimethyl sulfoxide inhibits acute and chronic bladder inflammation in transgenic experimental autoimmune cystitis models. Journal of biomedicine & biotechnology, 2011, 937061. PMID: 21113298.

Luo, Y., Henning, J. & O'Donnell, M. A. (2011). Th1 cytokine-secreting recombinant Mycobacterium bovis bacillus Calmette-Guérin and prospective use in immunotherapy of bladder cancer. Clinical & developmental immunology, 2011, 728930. PMID: 21941579.

Luo, Y., Han, R., Evanoff, D. P. & Chen, X. (2010). Interleukin-10 inhibits Mycobacterium bovis bacillus Calmette-Guérin (BCG)-induced macrophage cytotoxicity against bladder cancer cells. Clinical and experimental immunology, 160(3), 359-68. PMID: 20148913.

Luo, Y., Knudson, M. J. (2010). Mycobacterium bovis bacillus Calmette-Guérin-induced macrophage cytotoxicity against bladder cancer cells. Clinical & developmental immunology, 2010, 357591. PMID: 20862387.

Liu, W., O'Donnell, M. A., Chen, X., Han, R. & Luo, Y. (2009). Recombinant bacillus Calmette-Guérin (BCG) expressing interferon-alpha 2B enhances human mononuclear cell cytotoxicity against bladder cancer cell lines in vitro. Cancer immunology, immunotherapy : CII, 58(10), 1647-55. PMID: 19214503.

Liu, W., Deyoung, B. R., Chen, X., Evanoff, D. P. & Luo, Y. (2008). RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis model. Journal of autoimmunity, 30(4), 257-65. PMID: 18162370.

Chen, X., O'DONNELL, M. A. & Luo, Y. (2007). Dose-dependent synergy of Th1-stimulating cytokines on bacille Calmette-Guérin-induced interferon-gamma production by human mononuclear cells. Clinical and experimental immunology, 149(1), 178-85. PMID: 17517055.

Luo, Y., Chen, X. & O'Donnell, M. A. (2007). Mycobacterium bovis bacillus Calmette-Guérin (BCG) induces human CC- and CXC-chemokines in vitro and in vivo. Clinical and experimental immunology, 147(2), 370-8. PMID: 17223980.

Liu, W., Evanoff, D. P., Chen, X. & Luo, Y. (2007). Urinary bladder epithelium antigen induces CD8+ T cell tolerance, activation, and autoimmune response. Journal of immunology (Baltimore, Md. : 1950), 178(1), 539-46. PMID: 17182594.

Luo, Y., Yamada, H., Evanoff, D. P. & Chen, X. (2006). Role of Th1-stimulating cytokines in bacillus Calmette-Guérin (BCG)-induced macrophage cytotoxicity against mouse bladder cancer MBT-2 cells. Clinical and experimental immunology, 146(1), 181-8. PMID: 16968412.

Yamada, H., Odonnell, M. A., Matsumoto, T. & Luo, Y. (2005). Interferon-gamma up-regulates toll-like receptor 4 and cooperates with lipopolysaccharide to produce macrophage-derived chemokine and interferon-gamma inducible protein-10 in human bladder cancer cell line RT4. The Journal of urology, 174(3), 1119-23. PMID: 16094077.

Yamada, H., Luo, Y., Matsumoto, T. & O'Donnell, M. A. (2005). A novel expression of macrophage derived chemokine in human bladder cancer. The Journal of urology, 173(3), 990-5. PMID: 15711363.

Luo, Y. (2005). A potential modality for interstitial cystitis: Intravesical use of anti-inflammatory peptide RDP58. Journal of Urology, 173(2), 340. PMID: 15643170.

Luo, Y., Chen, X. & O'donnell, M. A. (2004). Use of prostate specific antigen to measure bladder tumor growth in a mouse orthotopic model. The Journal of urology, 172(6 Pt 1), 2414-20. PMID: 15538283.

Luo, Y., Yamada, H., Chen, X., Ryan, A. A., Evanoff, D. P., Triccas, J. A. & O'Donnell, M. A. (2004). Recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) expressing mouse IL-18 augments Th1 immunity and macrophage cytotoxicity. Clinical and experimental immunology, 137(1), 24-34. PMID: 15196240.

Ludwig, A. T., Moore, J. M., Luo, Y., Chen, X., Saltsgaver, N. A., O'Donnell, M. A. & Griffith, T. S. (2004). Tumor necrosis factor-related apoptosis-inducing ligand: a novel mechanism for Bacillus Calmette-Guérin-induced antitumor activity. Cancer research, 64(10), 3386-90. PMID: 15150089.

O'Donnell, M. A., Luo, Y., Hunter, S. E., Chen, X., Hayes, L. L. & Clinton, S. K. (2004). Interleukin-12 immunotherapy of murine transitional cell carcinoma of the bladder: dose dependent tumor eradication and generation of protective immunity. The Journal of urology, 171(3), 1330-5. PMID: 14767343.

O'Donnell, M. A., Luo, Y., Hunter, S. E., Chen, X., Hayes, L. L. & Clinton, S. K. (2004). The essential role of interferon-gamma during interleukin-12 therapy for murine transitional cell carcinoma of the bladder. The Journal of urology, 171(3), 1336-42. PMID: 14767344.

Chung, M. A., Luo, Y., O'Donnell, M., Rodriguez, C., Heber, W., Sharma, S. & Chang, H. R. (2003). Development and preclinical evaluation of a Bacillus Calmette-Guérin-MUC1-based novel breast cancer vaccine. Cancer research, 63(6), 1280-7. PMID: 12649188.

Nadler, R., Luo, Y., Zhao, W., Ritchey, J. K., Austin, J. C., Cohen, M. B., O'Donnell, M. A. & Ratliff, T. L. (2003). Interleukin 10 induced augmentation of delayed-type hypersensitivity (DTH) enhances Mycobacterium bovis bacillus Calmette-Guérin (BCG) mediated antitumour activity. Clinical and experimental immunology, 131(2), 206-16. PMID: 12562379.

Luo, Y., Chen, X. & O'Donnell, M. A. (2003). Role of Th1 and Th2 cytokines in BCG-induced IFN-gamma production: cytokine promotion and simulation of BCG effect. Cytokine, 21(1), 17-26. PMID: 12668155.

Young, S., O'Donnell, M., Lockhart, E., Buddle, B., Slobbe, L., Luo, Y., De Lisle, G. & Buchan, G. (2002). Manipulation of immune responses to Mycobacterium bovis by vaccination with IL-2- and IL-18-secreting recombinant bacillus Calmette Guerin. Immunology and cell biology, 80(3), 209-15. PMID: 12067407.

Luo, Y., Chen, X., Han, R. & O'Donnell, M. A. (2001). Recombinant bacille Calmette-Guérin (BCG) expressing human interferon-alpha 2B demonstrates enhanced immunogenicity. Clinical and experimental immunology, 123(2), 264-70. PMID: 11207657.

Luo, Y., Chen, X., Szilvasi, A. & O'Donnell, M. A. (2000). Co-expression of interleukin-2 and green fluorescent protein reporter in mycobacteria: in vivo application for monitoring antimycobacterial immunity. Molecular immunology, 37(9), 527-36. PMID: 11163402.

O'Donnell, M. A., Luo, Y., Chen, X., Szilvasi, A., Hunter, S. E. & Clinton, S. K. (1999). Role of IL-12 in the induction and potentiation of IFN-gamma in response to bacillus Calmette-Guérin. Journal of immunology (Baltimore, Md. : 1950), 163(8), 4246-52. PMID: 10510362.

Luo, Y., Chen, X., Han, R., Chorev, M., Dewolf, W. C. & O'Donnell, M. A. (1999). Mutated ras p21 as a target for cancer therapy in mouse transitional cell carcinoma. The Journal of urology, 162(4), 1519-26. PMID: 10492248.

Luo, Y., Chen, X., Downs, T. M., DeWolf, W. C. & O'Donnell, M. A. (1999). IFN-alpha 2B enhances Th1 cytokine responses in bladder cancer patients receiving Mycobacterium bovis bacillus Calmette-Guérin immunotherapy. Journal of immunology (Baltimore, Md. : 1950), 162(4), 2399-405. PMID: 9973521.

Luo, Y., Kurz, J., MacAfee, N. & Krause, M. O. (1997). C-myc deregulation during transformation induction: involvement of 7SK RNA. Journal of cellular biochemistry, 64(2), 313-27. PMID: 9027590.

Luo, Y., Szilvasi, A., Chen, X., DeWolf, W. C. & O'Donnell, M. A. (1996). A novel method for monitoring Mycobacterium bovis BCG trafficking with recombinant BCG expressing green fluorescent protein. Clinical and diagnostic laboratory immunology, 3(6), 761-8. PMID: 8914772.

Luo, Y., Krause, M. O. (1994). Changes in promoter utilization in human and mouse c-myc genes upon transformation induction in temperature-sensitive cell lines. Journal of cellular physiology, 160(2), 303-15. PMID: 8040189.

Krause, M., Luo, Y. (1994). Early transformation-specific events in the control of c-myc expression in mammalian cells. In Advances in Structural Biology. pp. 1-16. Jai Press, Inc.

Okazaki, M., Luo, Y., Han, T., Yoshida, M. & Seon, B. K. (1993). Three new monoclonal antibodies that define a unique antigen associated with prolymphocytic leukemia/non-Hodgkin's lymphoma and are effectively internalized after binding to the cell surface antigen. Blood, 81(1), 84-94. PMID: 8417805.

Luo, Y., Seon, B. K. (1990). Marked difference in the in vivo antitumor efficacy between two immunotoxins targeted to different epitopes of common acute lymphoblastic leukemia antigen (CD10). Mechanisms involved in the differential activities of immunotoxins. Journal of immunology (Baltimore, Md. : 1950), 145(6), 1974-82. PMID: 1697314.

Yokota, S., Hara, H., Luo, Y. & Seon, B. K. (1990). Synergistic potentiation of in vivo antitumor activity of anti-human T-leukemia immunotoxins by recombinant alpha-interferon and daunorubicin. Cancer research, 50(1), 32-7. PMID: 2293557.

Luo, Y., Hara, H., Haruta, Y. & Seon, B. K. (1989). Establishment of ascitic tumor of human pre-B acute lymphoblastic leukemia in nonconditioned nude mice. Cancer research, 49(3), 706-10. PMID: 2783384.

Hara, H., Luo, Y., Haruta, Y. & Seon, B. K. (1988). Efficient transplantation of human non-T-leukemia cells into nude mice and induction of complete regression of the transplanted distinct tumors by ricin A-chain conjugates of monoclonal antibodies SN5 and SN6. Cancer research, 48(16), 4673-80. PMID: 2969282.