Grants
2016. C. Andrew Frank, University of Iowa, in collaboration with Stephanie Toering Peters, Wartburg College. NSF "How FGF receptor signaling drives homeostatic neuroplasticity".
2016. JR Kirby, University of Iowa, in collaboration with Mary Shawgo, Graceland University. NSF "Integrative Organismal Systems Grant Application".
2016. Nalin Goonesekere, University of Northern Iowa, in collaboration with the Henry Laboratory. NSF-EPSCoR "Establishing the status of four novel candidate cancer genes as potential biomarkers in pancreatic cancer". $20,000
2016. Nalin Goonesekere, University of Northern Iowa, grants for Summer Undergraduate Research for student participants. $6000
2016. Nalin Goonesekere, University of Northern Iowa, grants for Summer Undergraduate Research for faculty hosts. $15,000
2016. Nalin Goonesekere, University of Northern Iowa, grants for undergraduate research. $1500
2016. Jerry Honts and Heidi Sleister, Drake University. Drake Faculty Development Grant "Tetrins: Ties that Bind". $2500
2016. Jerry Honts, Drake University. NASA-Iowa Space EPSCoR Research Infrastructure Development Grant "3D Printing of Mechanical Devices Using Biopolymers Engineered by Synthetic Biology. $10,000
2016. Jerry Honts and Maria Bohorquez, Drake University. Drake Faculty Development Grant "Morn Repeat Proteins: Elmer's Glue for Cells". $2500
2016. Jerry Honts, Drake University. NASA-Iowa Space EPSCoR Travel Grant. Visit to Ames Research Center, Moffett Field, CA. $6000
2016. Jerry Honts, Drake University. NASA-Iowa Space EPSCoR Research Infrastructure Development Grant "Using Synthetic Biology to Create a Calcium-Activated Contractile Fabric". $15,000
2016. Tina Tootle, Anatomy and Cell Biology, in support of Douglas Brusich, Wartburg College. NIH R01 "Prostaglandins Control Development by Coordinating Actin Cytoskeletal Remodeling".
2016. Ugur Akgun, Coe College, in collaboration with the Wang Laboratory. NIH R01 "Affordable Glass Detectors for Radiation Oncology". Under review.
2018. Randy Christensen, Coe College. NSF S-STEM "The STEMPath Approach: Success for Low Income Students". $650,000
Patent Applications
2016. Ugur Akgun, Coe College, in collaboration with the Wang Laboratory. Provisional patent application. "Apparatus, System, and Method for a Proton Beam Imager Using High-Density Glass Scintillator".
PDB Structures
2015. 4NXP. David Speckhard, Loras College, in collaboration with the Fuentes Laboratory. Crystal Structures of Free T-cell Lymphoma Invasion and Metastasis-1 PDZ Domain Quadruple Mutant (QM). DOI: 10.2210/pdb4nxp/pdb
2015. 4NXR. David Speckhard, Loras College, in collaboration with the Fuentes Laboratory. Crystal Structure of T-cell Lymphoma Invasion and Metastasis-1 PDZ Domain Quadruple Mutant (QM) in Complex with Neurexin-1 Peptide. DOI: 10.2210/pdb4nxr/pdb
2015. 4NXQ. David Speckhard, Loras College, in collaboration with the Fuentes Laboratory. Crystal Structure of T-cell Lymphoma Invasion and Metastasis-1 PDZ Domain Quadruple Mutant (QM) in Complex with Caspr4 Peptide. DOI: 10.2210/pdb4nxq/pdb
2016. 2NCO. Adina Kilpatrick, Drake University, in collaboration with the Shea Laboratory. NMR solution structure for the C-terminal domain of Tetrahymena Tcb2 in the absence of calcium. DOI: 10.2210/pdb2nco/pdb
2016. 2NCP. Adina Kilpatrick, Drake University, in collaboration with the Shea Laboratory. NMR solution structure for the C-terminal domain of Tetrahymena Tcb2 in the presence of calcium. DOI: 10.2210/pdb2ncp/pdb
Publications
http://www.ncbi.nlm.nih.gov/pubmed/?term=20625373
Lab Invest. 2010 Dec; 90(12):1727-36. doi: 10.1038/labinvest.2010.126. Epub 2010 Jul 12. Correlation between microRNA expression levels and clinical parameters associated with chronic hepatitis C viral infection in humans. Marquez RT, Bandyopadhyay S, Wendlandt EB, Keck K, Hoffer BA, Icardi MS, Christensen RN, Schmidt WN, McCaffrey AP.
https://www.ncbi.nlm.nih.gov/pubmed/22673745
Small GTPases. 2012 Jan-Mar; 3(1):15-22. doi: 10.4161/sgtp.19557. Epub 2012 Jan 1. Divergence of Rho residue 43 impacts GEF activity. Sloan CM, Quinn CV, Peters JP, Farley J, Goetzinger C, Wernli M, DeMali KA, Ellerbroek SM.
https://www.cell.com/biophysj/fulltext/S0006-3495(11)03593-4
Biophysical Journal. 2012 Jan 31; 102(3):411a. doi: 10.1016/j.bpj.2011.11.2245. Specificity, Structure and Dynamics of Tiam1 PDZ Domain Ligand-Bound Complexes. Fuentes EJ, Liu X, Shepherd TR, Speckhard DC, Murray AM.
https://www.ncbi.nlm.nih.gov/pubmed/?term=22915611
J Clin Microbiol. 2012 Nov; 50(11):3744-6. doi: 10.1128/JCM.01488-12. Epub 2012 Aug 22. Nostrils of healthy volunteers are independent with regard to Staphylococcus aureus carriage. Kildow BJ, Conradie JP, Robson RL.
http://www.sciencedirect.com/science/article/pii/B9780123786302002516
Nuclear Organization, Chromatin Structure, and Gene Silencing. Vitalini MW, Dialynas G, Wallrath LL, Mackey SR. Encyclopedia of Biological Chemistry (2013) pp. 314-317. Edited by WJ Lennarz and MD Lane.
http://www.ncbi.nlm.nih.gov/pubmed/?term=24282525
PLoS One. 2013 Nov 25; 8(11):e80228. doi: 10.1371/journal.pone.0080228. eCollection 2013. RABL6A, a novel RAB-like protein, controls centrosome amplification and chromosome instability in primary fibroblasts. Zhang X, Hagen J, Muniz VP, Smith T, Coombs GS, Eischen CM, Mackie DI, Roman DL, Van Rheeden R, Darbro B, Tompkins VS, Quelle DE.
https://www.cell.com/biophysj/fulltext/S0006-3495(13)04917-5
Biophysical Journal. 2014 Jan 28; 106(2):661a. doi: 10.1016/j.bpj.2013.11.3658. Structural and Dynamic Features Underlie the Switch of Ligand Binding Specificity in a Tiam 1 PDZ Domain Mutant. Fuentes EJ, Liu X, Speckhard DC, Shepherd TR.
http://www.ncbi.nlm.nih.gov/pubmed/25100706
Biol Reprod. 2014 Sep; 91(3):74. doi: 10.1095/biolreprod. 114.118562. Epub 2014 Aug 6. FSH Receptor (FSHR) Expression in Human Extra-Gonadal Reproductive Tissues and the Developing Placenta, and the Impact of Its Deletion on Pregnancy in Mice. Stilley JA, Christensen DE, Dahlem KB, Guan R, Santillan DA, England SK, Al-Hendy A, Kirby PA, Segaloff DL. PMID: 25100706.
http://www.ncbi.nlm.nih.gov/pubmed/?term=25145833
Biophys Chem. 2014 Sep-Oct; 193-194:35-49. doi: 10.1016/j.bpc.2014.07.004. Epub 2014 Jul 30. Calcium-dependent energetics of calmodulin domain interactions with regulatory regions of the Ryanodine Receptor Type 1 (RyR1). Newman RA, Sorensen BR, Kilpatrick AM, Shea MA.
http://www.ncbi.nlm.nih.gov/pubmed/25358683
Biochem J. 2014 Oct 31. [Epub ahead of print]. Vinculin-dependent actin bundling regulates cell migration and traction forces. Jannie KM, Ellerbroek SM, Zhou DW, Chen S, Crompton DJ, Garcia AJ, DeMali KA. PMID: 25358683.
Traffic. 2015 Sep 16. doi: 10.1111/tra. 12332. [Epub ahead ofr print]. Identification of a VxP targeting signal in the flagellar Na±/K±-ATPase. Laird JG, Pan Y, Modestou M, Yamaguchi DM, Song H, Sokolov M, Baker SA. PMID: 26373354.
Invest Ophthalmol Vis Sci. 2015 Jun; 56(6):3514-21. doi: 10.1167/iovs. 15-16902. An N-Terminal ER Export Signal Facilitates the Plasma Membrane Targeting of HCN1 Channels in Photoreceptors. Pan Y, Laird JG, Yamaguchi DM, Baker SA. PMID: 26030105.
Cell Mol Life Sci. 2015 Feb; 72(4): 833-43. doi: 10.1007/s00018-014-1705-1. Epub 2014 Aug 21. A di-arginine ER retention signal regulates trafficking of HCN1 channels from the early secretory pathway to the plasma membrane. Pan Y, Laird JG, Yamaguchi DM, Baker SA. PMID: 25142030.
https://www.cell.com/biophysj/fulltext/S0006-3495(15)03932-6
Biophysical Journal. 2016 Feb 16; 110(3):514a. doi: 10.1016/j.bpj.2015.11.2749. Conformational Dynamics and Structure Underlie the Novel Ligand Binding Specificity of a Tiam 1 PDZ Domain Mutant. Fuentes EJ, Liu X, Speckhard DC, Shepherd TR, Hengel SR, Yu L, Fowler CA, Gakhar L.
http://www.ncbi.nlm.nih.gov/pubmed/27155947
Biomolecular NMR Assignments. 2016 Oct; 10(2):281-5. doi: 10.1007/s1104-016-9684-9. Backbone and side-chain shift assignments for the C-terminal domain of Tcb2, a cytoskeletal calcium-binding protein from Tetrahymena thermophila. Kilpatrick AM, Gurrola TE, Sterner RC, Sleister HM, Honts JE, Fowler CA.
Microarrays. 2016 sub. Diagnostic, Prognostic and Predictive Cancer Biomarkers. Goonesekere N.
British Journal of Medicine and Medical Research. 2016 sub. Diagnostic, Prognostic and Predictive Cancer Biomarkers. Goonesekere N.
https://jb.asm.org/content/198/24/3335
Journal of Bacteriology. 2016 Oct 3. pii: JB.00575-16 [Epub ahead of print]. Identification of Functions Affecting Predator-Prey Interactions between Myxococcus and Bacillus subtilis. Mueller S, Strack SN, Ryan SE, Shawgo M, Walling A, Harris S, Chambers C, Boddicker J, Kirby JR.
http://www.ncbi.nlm.nih.gov/pubmed/27488393
Proteins. 2016 Nov; 84(11):1748-1758. doi: 10.1002/prot.25111. Solution NMR structures of the C-domain of Tetrahymena cytoskeletal protein Tcb2 reveal distinct calcium-induced structural rearrangements. Kilpatrick AM, Honts JE, Sleister HE, Fowler CA.
Orthopaedic Journal of Sports Medicine. 2017. High prevalence of non-traumatic shoulder pain in a regional sample of female high school volleyball athletes. Frisch K, Clark J, Hanson C, Fagerness C, Conway A, Hoogendoorn L.
https://doi.org/10.1111/pim.12443
Parasite Immunology. 2017; 39:e12443. doi: 10.1111/pim.12443. Lipid bodies accumulation in Leishmania infantum-infected C57BL/6 macrophages. Rodriguez NE, Lockard RD, Turcotte EA, et al.
Innate Immunity. 2017; 23(3) 307-318. Modification of sample processing for the Limulus amebocyte lysate assay enhances detection of inflammogenic endotoxin in intact bacteria and organic dust. Hoppe Parr KA, Hadina S, Kilburg-Basnyat B, Wang Y, Chavez D, Thorne PS, Weiss JP.
https://www.ncbi.nlm.nih.gov/pubmed/28823028
Biomol NMR Assign. 2017 Oct;11(2):297-303. doi: 10.1007/s12104-017-9767-2. Epub 2017 Aug 19. Backbone resonance assignments of complexes of human voltage-dependent sodium channel NaV1.2 IQ motif peptide bound to apo calmodulin and to the C-domain fragment of apo calmodulin. Mahling R, Kilpatrick AM, Shea MA
https://doi.org/10.1111/pim.12443
Parasite Immunol. 2017;39:e12443. Lipid bodies accumulation in Leishmania infantum-infected C57BL/6 macrophages. Rodríguez NE, Lockard RD, Turcotte EA, et al.
https://link.springer.com/article/10.1007%2Fs00432-017-2558-4Journal of Cancer Research and Clinical Oncology (2018) 144:309-320. Identification of genes highly downregulated in pancreatic cancer through a meta-analysis of microarray datasets: implications for discovery of novel tumor-suppressor genes and therapeutic targets. Goonesekere NC, Andersen W, Wang X.
https://link.springer.com/article/10.1007%2Fs12104-018-9824-5#citeas
Biomol NMR Assign. 2018 May 4. doi: 10.1007/s12104-018-9824-5. [Epub ahead of print] Backbone resonance assignments of complexes of apo human calmodulin bound to IQ motif peptides of voltage-dependent sodium channels NaV1.1, NaV1.4 and NaV1.7. Isbell HM, Kilpatrick AM, Lin Z, Mahling R, Shea MA
Selected Presentations
2013. Jerry Honts, Drake University. Talk at the Iowa Microscopy Society 2013 Fall Symposium, Iowa City, IA. GFP-tagged proteins identify distinct domains within the membrane skeleton of Tetrahymena thermophile.
2013. Jackie Brittingham and undergraduate researchers, Simpson College. Poster presentation at the Society for Developmental Biology Meeting, Washington University, St. Louis, MO. Wnt Pathway Disruptors Duplication of Axis in Nematostella vectensis. Besack M, Greaves J, Brttingham JW.
2013. Jackie Brittingham and undergraduate researchers, Simpson College. Talk at the Iowa Academy of Science Meeting, Simpson College, Indianola, IA. Characterization of Wnt Disruptors in Nematostella vectensis Development. Greaves J, Besack M, Brittingham JW.
2014. Stephanie Toering-Peters, Wartburg College. Talk at the Iowa Microscopy Society 2014 Fall Symposium, Iowa City, IA. Loss of Dgk impairs synapse structure and function.
2014. Ugur Akgun, Coe College. Talk at the 2014 Iowa Biochemistry Workshop "Computational Biophysics", Iowa City, IA. Binding Interactions between P-Glycoprotein and Cardiovascular Drugs.
2014. Adam Moser, Loras College. Talk at the 2014 Iowa Biochemistry Workshop "Computational Biophysics", Iowa City, IA. Computational Biophysics with Undergraduates.
2015. Heriberto Hernandez, Grinell College. Talk at the 2015 Iowa Biochemistry Workshop "Diversity in Biophysics", Iowa City, IA. Computational Studies of Condensed Phase Systems.
2015. Adina Kilpatrick, Drake University. Talk at the 2015 Iowa Biochemistry Workshop "Diversity in Biophysics", Iowa City, IA. Thermodynamic and Structural Analysis of Calmodulin Interaction with the Skeletal Muscle Ryanodine Receptor.
2015. Abebe Mengesha, Drake University. Talk at the Iowa Microscopy Society 2015 Fall Symposium, Iowa City, IA. Triggerable lipid-based local delivery of chemotherapy: Smart medical system.
2015. Adina M. Kilpatrick, Drake University, in collaboration with the Shea Laboratory. Talk at the 59th Annual Biophysical Society Meeting, Baltimore, MD. Thermodynamic and Structural Analysis of Calmodulin Interaction with the Skeletal Muscle Ryanodine Receptor. Biophysical Journal 108(2):178a.
2015. Adina M. Kilpatrick, Drake University, in collaboration with the Shea Laboratory. Talk at the 29th Annual Gibbs Conference on Biothermodynamics, Carbondale, IL. Thermodynamic and structural analysis of calmodulin interaction with the skeletal muscle ryanodine receptor.
2015. Gary Coombs, Waldorf College, in collaboration with the Wallrath Laboratory. Poster presentation at the 6th Annual Biochemistry Retreat, Iowa City, IA. Manipulating metabolic pathways to suppress muscular dystrophy.
2015. Nalin Goonesekere, University of Northern Iowa, in collaboration with the Henry Laboratory. Poster presentation at the National Meeting of the American Chemical Society.
2015. Nalin Goonesekere, University of Northern Iowa, in collaboration with the Henry Laboratory. Poster presentation at the 30th National Conference on Undergraduate Research.
2016. Julia Rumley and Alitza Shutt, student researchers from Grinnell College, presenting at the ACS National Meeting. March 13-17, 2016.
2016. John R Kirby, in collaboration with Mary Shawgo, Graceland University. Talk at the American Society for Microbiology (ASM) Conference on Biofilms. BLAST XIII: Bacterial Locomotion and Signal Transduction.
2016. John R Kirby, in collaboration with Mary Shawgo, Graceland University. Talk at the American Society for Microbiology (ASM) Microbe (Session Chair). Emergent Behaviors from Bacterial Interactions".
2016. Susanne Mueller, in collaboration with Mary Shawgo, Graceland University. Talk at the American Society for Microbiology (ASM) Conference on Biofilms. Mechanisms of Interbacterial Cooperation and Competition.
2016. Susanne Mueller, in collaboration with Mary Shawgo, Graceland University. Invited speaker at the American Society for Microbiology (ASM) Microbe.
2016. John R Kirby, in collaboration with Mary Shawgo, Graceland University. Talk at University of North Carolina.
2016. John R Kirby, in collaboration with Mary Shawgo, Graceland University. Talk at Duke University.
2016. John R Kirby, in collaboration with Mary Shawgo, Graceland University. Talk at Texas A&M University.
2016. Abebe Mengesha, Drake University. Iowa Microscopy Society meeting, Iowa City.