Erwin F. Shibata, PhD

Portrait
Teaching Assistant (Physiology and Biophysics)
Associate Professor of Molecular Physiology and Biophysics

Contact Information

Primary Office: 6-450 BSB
51 Newton Road
Iowa City, IA 52242
319-335-7836

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Education

BSEE, Electrical Engineering, University of Washington
MSE, Biomedical Engineering, University of Washington
PhD, Cardiac Electrophysiology, The University of Texas Medical Branch

Education/Training Program Affiliations

Department of Molecular Physiology and Biophysics PhD

Research Summary

The primary focus in my laboratory is to understand the regulatory mechanism(s) of ion channels in excitable cells. Specifically, we are interested in the mechanism by which beta-adrenergic and muscarinic receptor agonists modulate ionic currents. Previous studies have suggested that in addition to the phosphorylation-dependent effects on ion channels, Gsalpha has an additional “direct” effect on the increase of cardiac sodium current that is independent but concurrent with phosphorylation effects. Our results strongly suggest that the number of functional Na+ channels increases in the membrane and that these channels are in caveolae (specifically those with the caveolin-3 isoform) (Yarbrough et al., 2002). Caveolae are dynamic omega-shaped invaginations whose membrane fusion and fission mechanisms are virtually unknown. These channel proteins do not migrate out of the caveolar membrane domain. Na+ channels within caveolae become functional when the caveolae “neck” opens to establish electrical continuity between the extracellular space and the intra-caveolae compartment. Our studies focus on determining the co-localization of Na+ (Nav1.5), Ca2+ (L-type or Cav1.2), and K+ (Kv1.5) channels in caveolae and the role of Gsalpha in the regulation of caveolae neck opening and closing. Our recent findings show that Gsalpha contains a critical histidine residue that plays an obligatory role in the caveolae neck opening. Our animal models include enzymatically dissociated single myocytes from rabbit, rat and human hearts. We use several types of approaches to test our hypothesis. Some of these techniques include Western blot analysis, immunoprecipitation, confocal immunofluorescence, immuno-electron microscopy, and patch-clamping. The patch pipette voltage-clamp technique is used to determine changes in the biophysical properties of sodium, calcium and potassium currents as well as ultra high-resolution capacitance measurements. These studies will provide new information into the mechanism of caveolae in heart and direct pathways for pharmacological interventions and therapeutic modalities of lethal arrhythmias.

Publications

Besse, I. M., Mitchell, C. C., HUND, T. J. & Shibata, E. F. (2011). A Computational Investigation of Cardiac Caveolae as a Source of Persistent Sodium Current. (Vols. 2). pp. 87. Frontiers in Computational Physiology and Medicine.

Besse, I. M., Mitchell, C. C., Shibata, E. F. & HUND, T. J. (2009). A Modified Mitchell-Schaeffer Model of Cardiac Action Potentials: Incorporating Caveolae-Associated Ion Currents.

Besse, I. M., Mitchell, C. C., Shibata, E. F. & HUND, T. J. (2009). Modeling Caveolar Stochasticity: Proposing an alternative source of late, persistent sodium current.

Besse, I. M., Mitchell, C. C., Shibata, E. F. & HUND, T. J. (2009). Modeling Stochastic Caveolae: A potential surge of arrhythmogenic persistent sodium current in cardiomyocytes.

Palygin, O. A., Pettus, J. M. & Shibata, E. F. (2008). Regulation of Caveolar Cardiac Sodium Current by a Single Gsalpha Histidine Residue. (Vols. 294). pp. H1693-H1699. Am. J. of Physiol., Heart & Circ. Physiol.. PMID: 18281377.

Lowe, J. S., Palygin, O., Bhasin, N., HUND, T. J., Boyden, P. A., Shibata, E. F., Anderson, M. E. & MOHLER, P. J. (2008). Voltage-gated Nav channel targeting in heart requires an ankyrin-G-dependent cellular pathway. (Vols. 180). pp. 173-186. The Jouranl of Cell Biology. PMID: 18180363.

Besse, I. M., Mitchell, C. C. & Shibata, E. F. (2008). Modeling Cardiac Action Potential: Incorporating the Caveolae Associated Current.

Besse, I., Mitchell, C. & Shibata, E. F. (2008). A model of cardiac action potential: Incorporating the caveolae-associated sodium current.

Palygin, O. A., Pettus, J. A. & Shibata, E. F. (2007). Protein kinase A-independent and Gsalpha-Mediated Regulation of Caveolar Cardiac Sodium Channels by B-Adrenergic Stimulation.

Lowe, J. S., Palygin, O., Wright, P., Shibata, E. F. & MOHLER, P. J. (2007). An ankyrin-G-dependent pathway controls Nav1.5 membrane localization and expression in heart.

Shibata, E. F., Brown, T. L., Washburn, Z. W., Bai, J., Revak, T. J. & Butters, C. A. (2006). Autonomic Regulation of Voltage-Gated Cardiac Ion Channels. (Vols. 17). pp. S34-S42. J. Cardiovasc. Electrophysiol.. PMID: 16686680.

Palygin, O. A., Shibata, E. F. (2006). Protein Kinase A-Independent and Gsalpha-Mediated Regulation of Caveolar Cardiac Sodium Channels by B-Adrenergic Stimulation.

Yarbrough, T., Lu, T., Lee, H. C. & Shibata, E. F. (2002). Localization of Cardiac Sodium Channels in Caveolin-Rich Membrane Domains: Regulation of Sodium Current Amplitude. (Vols. 90). pp. 443-449. Circ. Res.. PMID: 11884374.

Padanilam, B. J., Lu, T., Hoshi, T., Padanilam, B. A., Shibata, E. F. & Lee, H. C. (2002). Molecular determinants of intracellular pH modulation of human Kv1.4 N-type inactivation. (Vols. 62). pp. 127-134. Molecular Pharmacology. PMID: 12065763.

Yarbrough, T. L., Lu, T., Lee, H. & Shibata, E. F. (2000). Role of Caveolae in the "Direct" Gs-alpha Modulation of Cardiac Sodium Current. (Vols. Suppl. 102). (18), pp. II-262. Circulation.

Padanilam, B. J., Lu, T., Hoshi, T., Padanilam, B. A., Shibata, E. F. & Lee, H. C. (1999). Molecular determinants of pH modulation of human Kv1.4 N-type inactivation. (Vols. 100). pp. I424. Circulation.

Lee, H. C., Lu, T., Weintraub, N. L., Spector, A. A. & Shibata, E. F. (1999). Mechanisms of voltage-dependent sodium channel inhibition by cytochrome P-450 arachidonic acid metabolites in rat ventricular myocytes. (Vols. 22). pp. 856. Pacing and Clinic Electrophysiology.

Padanilam, B. J., Hoshi, T., Padanilam, B. A., Shibata, E. F. & Lee, H. C. (1999). Molecular determinants of pH modulation of human Kv1.4 N-type inactivation. (Vols. 100). (18), pp. I-424. Circulation.

Lu, T., Lee, H. C., Kabat, J. A. & Shibata, E. F. (1999). Modulation of Rat Cardiac Sodium Channel by the Stimulatory G-Protein alpha-Subunit. (Vols. 518.2). pp. 371-384. J. Physiol. (Lond.). PMID: 10381586.

Lee, H. C., Lu, T., Weintraub, N. L., VanRollins, M., Spector, A. A. & Shibata, E. F. (1999). Effects of Epoxyeicosatrienoic Acids on the Cardiac Sodium Channels in Isolated Rat Ventricular Myocytes. (Vols. 519.1). pp. 153-168. J. Physiol. (Lond.). PMID: 10432346.

Lee, H. C., Lu, T., Weintraub, N. L., Spector, A. A. & Shibata, E. F. (1999). Effects of epoxyeicosatrienoic acids on the cardiac sodium channels in isolated rat ventricular myocytes. (Vols. 47). pp. 221A. Journal of Investigative Medicine.

Lee, H. C., Lu, T., Weintraub, N. L., Spector, A. A. & Shibata, E. F. (1999). Inhibition of voltage-dependent Na+ channels by 8,9 -epoxyeicosatrienoic acid in rat ventricular myocytes. (Vols. 76). pp. A80. Biophysical Journal.

Lu, T., Lee, H. & Shibata, E. F. (1998). Enhancement of rat cardiac sodium currents by G-protein alpha subunit. (Vols. 74). (2), pp. A328. Biophysical J..

Genous, L., Yarbrough, T. L., Padanilam, B. J., Lee, H. & Shibata, E. F. (1997). Effects of nifedipine on the cardiac transient outward K+ channel expressed in Xenopus oocytes. (Vols. 72). (2), pp. A263. Biophysical J..

Avdonin, V., Shibata, E. F. & Hoshi, T. (1997). Dihydropyridine Action on Voltage-Dependent Potassium Channels Expressed in Xenopus Oocytes. (Vols. 109). pp. 169-180. J. Gen. Physiol.. PMID: 9041446.

Padanilam, B. J., Shibata, E. F., Hoshi, T. & Lee, H. (1997). Cytoplasmic factors regulating the inactivation kinetics of the human Kv 1.4 channel. (Vols. 72). (2), pp. A263. Biophysical J..

Lee, H. C., Cai, J. J., Arnar, D. O., Shibata, E. F. & Martins, J. B. (1996). Mechanism of Alpha-2 Adrenergic Modulation of Canine Cardiac Action Potential. (Vols. 278). pp. 278-606. J. Pharm. and Expt. Therap.. PMID: 8768709.

Avdonin, V., Shibata, E. F. & Hoshi, T. (1996). Dihydropyridine action on Shaker potassium channels. (Vols. 70). (2), pp. A399. Biophysical J..

Murray, J., Shibata, E. F., Buresh, T. L., Picken, H., O'Meara, B. W. & Conklin, J. L. (1995). Nitric Oxide Modulates a Calcium-Activated Potassium Current in Muscle Cells from Opossum Esophagus. (Vols. 269). (4), pp. G606-G612. Am. J. Physiol.. PMID: 7485514.

George, M. J., Shibata, E. F. (1995). Regulation of Calcium-Activated Potassium Channels by S-Nitrosothiol Compounds and Cyclic Guanosine Monophosphate in Rabbit Coronary Artery Myocytes. (Vols. 43). (5), pp. 451-158. J. Investig. Med.. PMID: 8528756.

Kamath, A. S., Larson, K., Shibata, E. F. & Hoshi, T. (1995). Mechanism of dihydropyridnine block of Shaker potassium channels. (Vols. 21). pp. 1831. Society for Neuroscience.

Samson, R. A., Cai, J. J., Shibata, E. F., Martins, J. B. & Lee, H. C. (1995). Electrophysiological Effects of alpha2-Adrenergic Stimulation in Canine Cardiac Purkinje Fibers. (Vols. 268). (5), pp. H2024-H2035. Am. J. Physiol.. PMID: 7771552.

Lamb, F. S., Volk, K. A. & Shibata, E. F. (1994). A Calcium-Activated Chloride Current in Rabbit Coronary Artery Myocytes. (Vols. 75). pp. 742-750. Circ. Res.. PMID: 7923619.

Matsuda, J. J., Lee, H. C. & Shibata, E. F. (1993). Acetylcholien Reversal of Isoproterenol-Stimulated Sodium Currents in Rabbit Ventricular Myocytes. (Vols. 72). pp. 517-525. Circ. Res.. PMID: 8431982.

Lee, H., Matsuda, J. J., Reynertson, S., Martins, J. B. & Shibata, E. F. (1993). Reversal of Lidocaine Effects on Sodium Currents by Isoproterenol in Rabbit Hearts and Heart Cells. (Vols. 91). pp. 693-701. J. Clin. Invest.. PMID: 8381826.

Volk, K. A., Shibata, E. F. (1993). Single Delayed Rectifier Potassium Channels from Rabbit Coronary Artery Myocytes. (Vols. 264). (4), pp. H1146-H1153. Am. J. Physiol.. PMID: 8476092.

Samson, R. A., Shibata, E. F. & Lee, H. C. (1993). Alpha-2 Adrenergic-Mediated Electrophysiologic Effects in Isolated Canine Purkinje Fibers. (Vols. 64). pp. A208. Biophys. J..

Martins, J. B., Matsuda, J. J., Lee, H. & Shibata, E. F. (1993). Effects of Phenylephrine on /Na in Isolated Rabbit Ventricular Myocytes. (Vols. 16). (Part II), pp. 870A. PACE.

Lamb, F. S., Shibata, E. F. (1993). A Calcium-Activated Chloride Channel in Rabbit Coronary Artery Smooth Muscle Cells. (Vols. 72). (5), pp. 498. Am. J. Cardiology.

George, M. J., Volk, K. A. & Shibata, E. F. (1993). A Voltage-Dependent, Calcium-Activated Potassium Current in Rabbit Coronary Artery Vascular Smooth Muscle Cells. (Vols. 64). pp. A200. Biophys. J..

Lamb, F. S., Matsuda, J. J., Volk, K. A. & Shibata, E. F. (1993). A Calcium-Activated Chloride Current in Rabbit Coronary Artery Myocytes. (Vols. 64). pp. A96. Biophys. J..

Matsuda, J. J., Lee, H. C., Reynertson, S. I., Martins, J. B. & Shibata, E. F. (1992). Reversal of Lidocaine Effects on Sodium Currents by Isoproterenol in Rabbit Hearts and Heart Cells. (Vols. 86). (4 (Supplement I)), pp. I-7. Circulation.

Matsuda, J. J., Lee, H., Reynertson, S. & Shibata, E. F. (1992). Isoproterenol Reverses Lidocaine Inhibition of Sodium Currents in Rabbit Cardiac Ventricular Myocytes. (Vols. 61). pp. A305. Biophysical J..

Shibata, E. F., Matsuda, J. J., Volk, K. A., Collison, K. A. & Segaloff, D. l. (1992). Evidence That the FSH Receptor Itself is Not a Calcium Channel. (Vols. 131). (2), pp. 979-981. Endocrinology. PMID: 1379170.

Matsuda, J. J., Lee, H. C. & Shibata, E. F. (1992). Enhancement of Rabbit Cardiac Sodium Channels by B-Adrenergic Stimulation. (Vols. 70). pp. 199-207. Circ. Res.. PMID: 1309315.

Gotoh, Y., Imaizumi, Y., Watanabe, M., Shibata, E. F., Clark, R. B. & Giles, W. R. (1991). Inhibition of a Transient Outward K+ Current by DHP Ca2+ Antagonists and Agonists in Rabbit Cardiac Myocytes. (Vols. 260). (5), pp. H1737-H1742. Am J. Physiol.. PMID: 1709794.

Volk, K. A., Matsuda, J. J. & Shibata, E. F. (1991). A Voltage-Dependent Potassium Current in Rabbit Coronary Artery Smooth Muscle Cells. (Vols. 439). pp. 751-768. J. Physiol. (Lond). PMID: 1910087.

Matsuda, J. J., Lee, H. & Shibata, E. F. (1991). The Effect of Acetylcholine on Sodium Currents in Rabbit Ventricular Myocytes. (Vols. 84). (Supplement II), pp. 175. Circulation.

Volk, K. A., Matsuda, J. J. & Shibata, E. F. (1991). Single Potassium Channels Currents from Vascular Smooth Muscle Cells of Rabbit Coronary Artery.

Matsuda, J. J., Lee, H. & Shibata, E. F. (1991). Modulation of Cardiac Sodium Currents by G-Proteins and cAMP-Dependent Phosphorylation.

Matsuda, J. J., Lee, H. C. & Shibata, E. F. (1990). ß-Adrenergic Modulation of Sodium Currents from Rabbit Cardiac Myocytes. (Vols. 57). pp. 140a. Biophysical J..

Lee, H. C., Matsuda, J. J., Lemmer, J. H. & Shibata, E. F. (1990). Regulation of Sodium Currents by ß-Adrenergic Stimulation in Isolated Cardiac Myocytes from Rabbit and Human. (Vols. 22). (Supplement I), pp. S.14. J. Mol. Cell. Cardiol..

Matsuda, J. J., Lee, H. C. & Shibata, E. F. (1990). Sodium Current Modulation of Autonomic Transmitters in Isolated Rabbit Cardiac Myocytes. (Vols. 15). pp. 144A. J. Am. Coll. Cardiol..

Rasmusson, R., Clark, J. W., Giles, W. R., Shibata, E. F. & Campbell, D. N. (1990). A Mathematical Model of a Bullfrog Cardiac Pacemaker Cell. (Vols. 259). pp. H352-H369. Am. J. Physiol.. PMID: 2167020.

Matsuda, J. J., Lee, H. C. & Shibata, E. F. (1990). Mechanism of Cardiac Sodium Currents Stimulation by Isoproterenol. (Vols. 82). (Supplement III), pp. 521. Circulation.

Shibata, E. F., Matsuda, J. J. & Volk, K. A. (1990). Calcium Currents in Rabbit Coronary Artery Smooth Muscle Cells. (Vols. 57). pp. 527a. Biophysical J..

Rasmusson, R., Clark, J. W., Giles, W. R., Robinson, K., Campbell, D. N., Clark, R. B. & Shibata, E. F. (1990). A Mathematical Model of Electrical Activity in the Bullfrog Atrial Cell. (Vols. 259). pp. H370-H389. Am. J. Physiol.. PMID: 2386218.

Volk, K. A., Matsuda, J. J. & Shibata, E. F. (1990). A Voltage-Dependent Potassium Current from Rabbit Coronary Artery Vascular Smooth Muscle Cells. (Vols. 57). pp. 512a. Biophysical J..

Matsuda, J. J., Volk, K. A. & Shibata, E. F. (1990). Calcium Currents in Isolated Rabbit Coronary Arterial Smooth Muscle Myocytes. (Vols. 427). pp. 657-680. J. Physiol. (Lond). PMID: 1698983.

Giles, W. R., Nakajima, T., Ono, K. & Shibata, E. F. (1989). Modulation of the Delayed Rectifier K+ Current by Isoprenaline in Bullfrog Atrial Myocytes. (Vols. 415). pp. 233-249. J. Physiol. (Lond). PMID: 2640462.

Nakajima, T., Ono, K., Shibata, E. F. & Giles, W. (1989). Modulation of K+ and Ca2+ Current by Isoproterenol in Bullfrog Atrial Myocytes. (Vols. 55). pp. 294a. Biophysical J..

Shibata, E. F., Drury, T., Refsum, H., Aldrete, V. & Giles, W. (1989). Contribution of a Transient Outward Current to Repolarization in Human Atrium. (Vols. 257). pp. H1773-H1781. Am. J. Physiol.. PMID: 2557769.

Campbell, D. N., Giles, W. & Shibata, E. F. (1988). Ion Transfer Characteristics of the Calcium Current in Bullfrog Myocytes. (Vols. 403). pp. 239-266. J. Physiol. (Lond). PMID: 2855341.

Campbell, D. N., Giles, W. R., Hume, J. R. & Shibata, E. F. (1988). Inactivation of Calcium Current of Bullfrog Atrial Myocytes. (Vols. 403). pp. 287-315. J. Physiol. (Lond). PMID: 2855343.

Campbell, D. N., Giles, W. R., Robinson, K. & Shibata, E. F. (1988). Studies of the Sodium-Calcium Exchanger in Bullfrog Atrial Myocytes. (Vols. 403). pp. 317-340. J. Physiol. (Lond). PMID: 2855344.

Giles, W., Imaizumi, Y., Clark, R. B., Shibata, E. F., van Ginneken, A. & West, G. A. (1988). Pacemaker Activity and Action Potentials in Mammalian Heart Cells. (Vols. 18.1). pp. 17. Engineering in Medicine and Biology, ACEMB 40th Annual Meeting of I.E.E.E..

Kavanagh, K. M., Giles, W. R., Rahmberg, M., Shibata, E. F., Robinson, K., Ilnicki, T., Aldrete, V. & Duff, H. J. (1988). Antiarrhythmic and electrophysiologic effects of barium inward rectifying potassium current blockade. (Vols. 11). (2A), pp. 59a. J. Am. Coll. Cardiol..

Campbell, D. N., Giles, W. R., Hume, J. R., Noble, D. & Shibata, E. F. (1988). Reversal Potential of the Calcium Current in Bullfrog Atrial Myocytes. (Vols. 403). pp. 267-286. J. Physiol. (Lond). PMID: 2855342.

Matsuda, J. J., Volk, K. A., Sharma, R. J., Bhalla, R. & Shibata, E. F. (1988). Calcium Currents from Isolated Bovine Carotid Artery Smooth Muscle Cells. (Vols. 53). pp. 554a. Biophysical J..

Bhalla, R., Bendhack, L. M., Sharma, R. V., Noel, M., Matsuda, J. J. & Shibata, E. F. (1988). Effect of Forskolin and Phorbol Esters on Ca2+ Currents, [Ca2+]i and Contraction of Enzymatically Dispersed VSM Cells. (Vols. 2). pp. A1188. FASEB J..

Giles, W., Shibata, E. F., Clark, J., Rasmusson, R., Murphey, R. & Momose, Y. (1988). Cardiac Pacemaker Activity in Single Cells from Bullfrog Sinus Venosus. In U.S.-Japan Seminar on Mechanisms Regulating Initiation and Conduction of the Cardiac Impulse. Okazaki National Institute for Physiological Sciences.

Shibata, E. F., Northup, J. K., Momose, Y. & Giles, W. (1987). Is Diacylgylcerol a Second Messenger for the Muscarinic Guanine Nucleotide Mediated Inhibition of Calcium Current in Bullfrog Atrium. (Vols. 51). pp. 413a. Biophysical J..

Shibata, E. F., Northup, J. K., Momose, Y. & Giles, W. (1986). Muscarinic Receptor and Guanine Nucleotide Mediated Inhibition of ICa in Single Cells from Bullfrog Atrium. (Vols. 49). pp. 349a. Biophysical J..

Hume, J. R., Giles, W., Robinson, K., Shibata, E. F., Nathan, R., Kanai, K. & Rasmusson, R. (1986). Time- and Voltage-Dependent Potassium Current in Single Cardiac Cells Isolated From Bullfrog Atrium. (Vols. 88). pp. 777-798. J. Gen. Physiol.. PMID: 2432158.

Shibata, E. F., Refsum, H., Aldrete, V., Ilnicki, T. & Giles, W. (1986). A Transient Outward Current in Single Cells from Human Atrial Muscle. (Vols. 74 (Supplement II):). pp. II-254. Circulation.

Moore, L. E., Clark, R. B., Shibata, E. F. & Giles, W. R. (1986). Comparison of the Steady-State Electrophysiological Properties of Isolated Cells From Bullfrog Atrium and Sinus Venosus. (Vols. 89). pp. 131-138. J. Memb. Biol.. PMID: 3486295.

Giles, W., van Ginneken, A. & Shibata, E. F. (1986). Ionic Currents Underlying Cardiac Pacemaker Activity: A Summary of Voltage Clamp Data From Single Cells. In R. D. Nathan (Eds.) Cardiac Muscle: Regulation of Excitation and Contraction. Academic Press, Orlando.

Campbell, D. N., Shibata, E. F., Robinson, K. & Giles, W. R. (1985). Experimental Theoretical Studies of the Electrogenic Na+/Ca2+ Exchanger in Bullfrog Atrial Myocytes. (Vols. 45). pp. 4601. Biophysical J..

Shibata, E. F., Giles, W. & Pollack, G. H. (1985). Theshold Effects of Acetylcholine on Primary Pacemaker Cells of the Rabbit Sino-Atrial Node. (Vols. 223). pp. 355-378. Proc. Royal Society (B). PMID: 2858102.

Shibata, E. F., Giles, W. (1985). Ionic Currents Which Generate the Spontaneous Diastolic Depolarization in Individual Cardiac Pacemaker Cells. (Vols. 82). pp. 7796-7800. Proc. Natl. Acad. Sci.. PMID: 3877934.

Moore, L. E., Shibata, E. F., Clark, R. B. & Giles, W. R. (1985). Steady State Properties of Isolated Frog Atrial and Sinus Venosus Cells. (Vols. 47). pp. 499a. Biophysical J..

Shibata, E. F., Giles, W. & Pollack, G. H. (1985). Threshold effects of acetylcholine on primary pacemaker cells of the rabbit sino-atrial node. Proceedings of the Royal Society of London. Series B, Biological sciences, 223(1232), 355-78. PMID: 2858102.

Giles, W., Shibata, E. F. (1985). Voltage Clamp of Isolated Cardiac Pacemaker Cells from Bullfrog Sinus Venosus: A Quantitative Analysis of Potassium Currents (Ph.D. Thesis). (Vols. 368). pp. 265-292. J. Physiol (Lond). PMID: 2416914.

Giles, W., Robinson, K., Shibata, E. F. & Hume, J. R. (1984). Repolarization in Cardiac Muscle: An Experimental and Theoretical Study Using Single Cells from Bullfrog Atrium. (Vols. 45). pp. 278a. Biophysical J..

Shibata, E. F., Momose, Y. & Giles, W. (1984). Nitrendipine Actions on Atrial Tissue in the Heart. In A. Scriabine , S. Vanov & K. Deck (Eds.) Nitrendipine. Urban and Schwarzenberg Ltd., Baltimore.

Shibata, E. F., Giles, W. (1984). Cardiac Pacemaker Cells from Bullfrog Sinus Venosus Lack an Inwardly Rectifying Background K+ Current. (Vols. 45). pp. 136a. Biophysical J..

Shibata, E. F., Momose, Y. & Giles, W. (1984). An Electrogenic Na+/K+ Pump Current in Individual Bullfrog Atrial Myocytes. (Vols. 45). pp. 136a. Biophysical J..

Giles, W., Shibata, E. F. & Smith, G. (1983). A TTX-Insensitive Transient Inward Current in Isolated Myocytes From Rabbit Atrium. (Vols. 345). pp. 151P. J. Physiol..

Shibata, E. F., Giles, W. (1983). Ionic Currents in Isolated Cardiac Pacemaker Cells in Bullfrog Sinus Venosus. (Vols. XV). pp. 167.07. Proc. Int. Union Physiol..

Shibata, E. F., Giles, W. (1983). Transmembrane Ionic Currents in Single Cardiac Pacemaker Cells. (Vols. 41). pp. 310a. Biophysical J..

Shibata, E. F., Momose, Y. & Giles, W. (1983). Measurement of Electrogenic Na+/K+ Pump Current in Individual Bullfrog Atrial Myocytes. (Vols. XV). pp. 138.17. Proc. Int. Union Physiol..

Giles, W., Hume, J. R. & Shibata, E. F. (1983). Presynaptic and Postsynaptic Actions of Cadmium in Cardiac Muscle. (Vols. 42). pp. 1994-1997. Fed Proc.. PMID: 6311634.

Giles, W., Momose, Y. & Shibata, E. F. (1983). Nitrendipine Actions on Transmembrane Ca++ and K+ Currents in Isolated Atrial Cells. (Vols. 26). pp. A-90. The Physiologist.

Giles, W., Shibata, E. F. (1981). Autonomic Transmitter Actions on Cardiac Pacemaker Tissue: A Brief Review. (Vols. 40). pp. 2618-2624. Fed. Proc.. PMID: 6115774.

Shibata, E. F., Giles, W. (1981). Effects of Ni2+ and Muscarine on Primary Pacemaker Cells in Rabbit Sino-Atrial Node. (Vols. 33). pp. 34a. Biophysical J..

Shibata, E. F., Giles, W. (1980). Threshold Effects of Autonomic Transmitters on Primary Pacemaker Cells in Rabbit Sino-Atrial Node. (Vols. 39). pp. 2483. Fed. proc..

Pollack, G. H., Iwazumi, T., ter Keurs, H. D. & Shibata, E. F. (1977). New Evidence Against the Cross-Bridge Theory. (Vols. 9). pp. 45. J. Mol. Cell. Cardiol..

Pollack, G. H., Iwazumi, T., ter Keurs, H. E. & Shibata, E. F. (1977). Sarcomere Shortening in Striated Muscle Occurs in Stepwise Fashion. (Vols. 268). pp. 757-759. Nature. PMID: 895878.

Pollack, G. H., Iwazumi, T., ter Keurs, H. E. & Shibata, E. F. (1977). Does Sarcomere Shortening Occur in Stepwise Fashion?. (Vols. 17). pp. 199a. Biophysical J..