The following commonly used primers are provided free of charge by the IIHG Genomics Division for use in automated sequencing. If you would like us to provide one of these primers, please indicate this when you complete your on-line request form by selecting a primer with the “CORE” designation and we will then add the primer requested to the DNA sample.

Please verify that the primer sequence of the selected primer coincides with that listed for your specific vector. We have found at least one instance where primers with the same name have entirely different sequences.

Universal Primers

• M13 (-20) FORWARD (BEN) 5'-TGT AAA ACG ACG GCC AGT-3'
• M13 FORWARD (RON) 5'-GTT TTC CCA GTC ACG ACG-3'
• M13 REVERSE (MARCY) 5'-CAG GAA ACA GCT ATG ACC-3'
• M13 REVERSE (NANCY) 5'-GTG TGG AAT TGT GAG CGG -3'
• SK 5'-TCT AGA ACT AGT GGA TC-3'
• KS 5'-CGA GGT CGA CGG TAT CG-3'
• T3 (EXT) 5'-G CGC AAT TAA CCC TCA CTA AAG-3'
• T7 5'-TAA TAC GAC TCA CTA TAG GG-3'
• SP6 5'-CGA TTT AGG TGA CAC TAT AG-3'

Vector Core Primers

• CMVp Forward (CMV promoter at base 544) 5’-GTG GGA GGT CTA TAT AAG CAG AGC TCG-3’
• PolyA Reverse (SV40 polyA at base 193) 5’-TTA AAA AAC CTC CCA CAC CTC CCC-C-3’
• Ad5 Forward 298 (Ad5 0-1 m.u. at base 298) 5’-ACT GAA TAA GAG GAA GTG AAA-3’
• RSV Forward (RSV promoter at base 215) 5’-CAT GCC GAT TGG TGG AAG TAA-G-3’
• mCMVp Forward (Modified CMV promoter at base 493) 5’-ATG TCG TAA CAA CTC CGC C-3’
• IRES Reverse (IRES at base 114) 5’- CTC ACA TTG CCA AAA GAC G-3’
• pFBAAV 5ITRrev 5'- GGC TTG CTG TTC TTC TAC GG -3'
• pFBAAV 3ITRrev 5'- GCC AAG TGG GCA GTT TAC-3'
• BgHpAF 5'- TGA GGA AAT TGC ATC GCA TTG TCT -3'
• BgHpAR 5'- AGG AAA GGA CAG TGG GAG TG -3'

Contact Information

116 EMRB
Phone: (319) 335-6736
Fax: (319) 335-6737
dna-sequencing@uiowa.edu

Samples for sequencing may be dropped off at 116 EMRB anytime from 8:00 AM to 5:00 PM Monday-Friday.