Toshihiro Kitamoto, PhD

Portrait
Associate Professor of Anesthesia
Associate Professor of Pharmacology
Associate Professor

Contact Information

Primary Office
1-316 BSB
51 Newton Road
Iowa City, IA 52242
319-356-2633

Education

BS, Biochemistry, University of Tokyo
MS, Biochemistry, University of Tokyo
PhD, Biochemistry, University of Tokyo
Postdoctoral Fellow, National Institute of Neuroscience
Fellow, Beckman Research, Institute of the City of Hope
Fellow, Beckman Research, Institute of the City of Hope

Education/Training Program Affiliations

Interdisciplinary Graduate Program in Genetics, Interdisciplinary Graduate Program in Neuroscience, Medical Scientist Training Program

Research Summary

Our lab is interested in understanding the genetic basis of complex behaviors or brain functions, such as learning and memory, sleep and mood. In order to elucidate fundamental mechanisms underlying the control of these behaviors, we take advantage of the power of the Drosophila (fruit fly) genetics, and study the relationship between genes and behavior at the molecular, cellular, systems and whole animal levels. Currently, our main research projects are on 1) the role of steroid hormones in the regulation of sleep and memory, and 2) the neurological pathways that are affected by the mood stabilizing drug lithium that is used for bipolar disorder. By examining the behavior of well-defined Drosophila mutants and transformants, we study the function of particular genes and their genetic interactions in fundamental aspects of behavior. Molecular and anatomical analyses are also being carried out to reveal the mechanisms employed by particular genes in the regulation of behavior. Our recent findings include 1) the insect molting hormone ecdysteroid promotes sleep and regulates long-term memory in adult flies, and 2) the Ca 2+/CaM-dependent phosphatase calcineurin is involved in the lithium's action in the nervous system and a novel SLC6 transporter plays a critical role in resistance to lithium toxicity. Our basic research is expected to provide new insights into the evolutionarily conserved mechanisms underlying higher-order brain functions that control complex behavior. PubMed link to publications: http://www.ncbi.nlm.nih.gov/pubmed?term=(((Kitamoto%20AND%20drosophila))%20OR%20(Kitamoto%20AND%20iowa))%20AND%20(%222004%22%5BDate%20-%20Publication%5D%20%3A%20%223000%22%5BDate%20-%20Publication%5D)

Publications

Mrkvicka III, J., Kasuya, J. & Kitamoto, T. (2018). A forward genetic screen to identify modifiers of neurological phenotypes in Drosophila voltage-gated sodium channel.

Lansdon, P., Kasuya, J. & Kitamoto, T. (2018). Gut microbiota influences the severity of neurological phenotypes in Drosophila voltage-gated sodium channel mutants.

Mrkvicka III, J., Chen, H., Kaas, G. A., Kasuya, J. & Kitamoto, T. (2018). A forward genetic screen to identify modifiers of neurological phenotypes in Drosophila voltage-gated sodium channel.

Lansdon, P., Kasuya, J. & Kitamoto, T. (2018). Gut microbiota influences the severity of neurological phenotypes in Drosophila voltage-gated sodium channel mutants.

Hindle, S. J., Munji, R. N., Dolghih, E., Gaskins, G., Orng, S., Ishimoto, H., Soung, A., DeSalvo, M., Kitamoto, T., Keiser, M. J., Jacobson, M. P., Daneman, R. & Bainton, R. J. (2017). Evolutionarily conserved roles for blood-brain barrier xenobiotic transporters in endogenous steroid partitioning and behavior. Cell Reports, 21(5), 1304-16. PMID: 29091768.

Lansdon, P., Kasuya, J. & Kitamoto, T. (2017). The role of gut microbiota and the antioxidant system in the seizure-like behavior of Shudderer, a Drosophila voltage-gated sodium channel mutant.

Lark, A., Kitamoto, T. & Martin, J. R. (2017). Modulation of neuronal activity in the Drosophila mushroom body by DopEcR, a unique dual receptor for ecdysone and dopamine. Biochimica et biophysica acta, 1864(10), 1578-88. PMID: 28554773.

Mrkvicka III, J., Chen, H. L., Kasuya, J. & Kitamoto, T. (2017). Identification of seizure suppressors through an unbiased forward genetic screen in a Drosophila model of epilepsy.

Lansdon, P., Chen, H. L., Kasuya, J. & Kitamoto, T. (2017). Gut microbiota and the antioxidant system are involved in the seizure-like behavior of Shudderer, a Drosophila voltage-gated sodium channel mutant.

Shimada, N., Inami, S., Sato, S., Kitamoto, T. & Sakai, T. (2016). Modulation of light-driven arousal by LIM-homeodomain transcription factor Apterous in large PDF-positive lateral neurons of the Drosophila brain. Scientific reports, 6, 37255. PMID: 27853240.

Kaas, G. A., Kasuya, J., Lansdon, P., Ueda, A., Iyengar, A., Wu, C. & Kitamoto, T. (2016). Lithium-Responsive Seizure-Like Hyperexcitability Is Caused by a Mutation in the "Drosophila" Voltage-Gated Sodium Channel Gene "paralytic". eNeuro, 3(5). PMID: 27844061.

Iyengar, A., Ueda, A., Kasuya, J., Gratz, S., O'Connor-Giles, K., Kitamoto, T. & Wu, C. (2016). Neuronal hyperexcitability and seizure-like motor pattern generation arising from the shaker wings-down mutation in the Drosophila sodium channel gene paralytic.

Ehaideb, S. N., Wignall, E. A., Kasuya, J., Evans, W. H., Iyengar, A., Koerselman, H. L., Lilienthal, A. J., Bassuk, A. G., Kitamoto, T. & Manak, J. R. (2016). Mutation of orthologous prickle genes causes a similar epilepsy syndrome in flies and humans. Annals of clinical and translational neurology, 3(9), 695-707. PMID: 27648459.

Petruccelli, E., Li, Q., Rao, Y. & Kitamoto, T. (2016). The unique dopamine/ecdysteroid receptor DopEcR modulates ethanol-induced sedation in Drosophila. The journal of neuroscience, 36(16), 4647-57. PMID: 27098705.

Lark, A. R., Kitamoto, T. & Martin, J. R. (2016). In vivo functional brain imaging approach based on bioluminescent calcium indicator GFP-Aequorin. Journal of visualized experiments,(107). PMID: 26779599.

Lark, A., Kitamoto, T. & Martin, J. (2016). Analysis of regulatory elements in the 5’ flanking region of the Drosophila choline acetyltransferase gene.

Petruccelli, E., Lansdon, P. & Kitamoto, T. (2015). Exaggerated nighttime sleep and defective sleep homeostasis in a Drosophila knock-in model of human epilepsy. PloS one, 10(9), e0137758. PMID: 26361221.

Petruccelli, E., Lansdon, P. & Kitamoto, T. (2015). Sleep architecture and seizure susceptibility of Drosophila voltage-gated sodium channel mutants that mimic human epilepsy syndrome.

Inami, S., Shimada, N., Asano, T., Kitamoto, T. & Sakai, T. (2015). Drosophila LIM homeodomain transcription factor Apterous is expressed in adult mushroom body and PDF neurons to play differential roles in processing long-term courtship memory.

Lark, A., Kitamoto, T. & Martin, J. (2015). Characterization of DopEcR, a unique dual receptor for ecdysone and dopamine, by in vivo functional brain imaging.

Lark, A., Kitamoto, T. & Martin, J. (2015). Characterization of DopEcR, a unique dual receptor for ecdysone and dopamine, by in vivo functional brain imaging.

Petruccelli, E., Lansdon, P. & Kitamoto, T. (2015). Sleep abnormalities in a Drosophila knock-in model of human generalized epilepsy with febrile seizure-plus (GEFS+).

Chen, H. L., Lansdon, P., Kasuya, J. & Kitamoto, T. (2015). Suppression of Gadd45 and stress-activated protein kinase pathways reduces severity of neurological phenotypes displayed by a voltage-gated sodium channel Drosophila mutant, Shudderer.

Kasuya, J., Lansdon, P., Chen, H. L. & Kitamoto, T. (2015). Effects of diet on neurological phenotypes of a voltage-gated sodium channel mutant, Shudderer.

Lansdon, P., Petruccelli, E. & Kitamoto, T. (2015). Effects of diet on Shudderer, a Drosophila voltage-gated sodium channel mutant that displays seizure-like behaviors.

Sato, S., Kitamoto, T. & Sakai, T. (2014). Modulation of innate and learned sexual behaviors by the TRP channel Painless expressed in the fruit fly brain: behavioral genetic analysis and its implications. Frontiers in behavioral neuroscience, 8, 400. PMID: 25520634.

Hindle, S., Erng, S., DeSalvo, M., Dolgikh, E., Ishimoto, H., Kitamoto, T., Jacobson, M. & Bainton, R. (2014). The blood-brain barrier regulates ecdysone signaling in the CNS, affecting neurophysiology and behavior.

Gutierrez, G., Krantz, D., Kendall, N., Kitamoto, T. & Lawal, H. (2014). Changes in acetylcholine release alter locomotion and touch response timing in Drosophila.

Sakai, T., Watanabe, K., Ohashi, H., Sato, S., Inami, S., Shimada, N. & Kitamoto, T. (2014). Insulin-producing cells regulate the sexual receptivity through the painless TRP channel in Drosophila virgin females. PloS one, 9(2), e88175. PMID: 24505416.

Petruccelli, E., Kitamoto, T. (2014). Drosophila Dopamine ecdysone receptor (DopEcR) modulates ethanol-induced sedation and hyperactivity.

Blake, A., Kendall, N., Boppana, S., Kitamoto, T. & Lawal, H. (2014). Effects of changes in acetylcholine release on locomotion states in central synapses. Society for Neuroscience - Abstracts.

Petruccelli, E., Lark, A., Rao, Y., Ishimoto, H. & Kitamoto, T. (2013). Drosophila DopEcR, a G-protein coupled receptor for ecdysteroids and dopamine, controls behavioral responses to ethanol.

Kitamoto, T. (2013). A novel role for ecdysone in Drosophila conditioned behavior.

Hindle, S., Orng, S., DeSalvo, M., Dolgikh, E., Ishimoto, H., Mayer, F., Kitamoto, T., Jacobson, M. & Bainton, R. (2013). ABS Transporters regulate CNS chemoprotection through endogenous ecdysone signaling at the BBB.

Ishimoto, H., Kitamoto, T. (2013). A novel action of the steroid hormone ecdysone on behavioral plasticity in adult Drosophila.

Ishimoto, H., Wang, Z., Rao, Y., Wu, C. & Kitamoto, T. (2013). A novel role for ecdysone in Drosophila conditioned behavior: linking GPCR-mediated non-canonical steroid action to cAMP signaling in the adult brain. PLoS genetics, 9(10), e1003843. PMID: 24130506.

Sakai, T., Sato, S., Ishimoto, H. & Kitamoto, T. (2012). Significance of the centrally expressed TRP channel painless in Drosophila courtship memory. Learning & memory, 20(1), 34-40. PMID: 23247253.

Sakai, T., Inami, S., Sato, S. & Kitamoto, T. (2012). Fan-shaped body neurons are involved in period-dependent regulation of long-term courtship memory in Drosophila. Learning & memory, 19(12), 571-4. PMID: 23154928.

Petruccelli, E., Kitamoto, T. (2012). The membrane-bound ecdysteroid receptor DopEcR plays a unique role in the regulation of behavioral response to ethanol in Drosophila.

Ishimoto, H., Lark, A. & Kitamoto, T. (2012). Factors that Differentially Affect Daytime and Nighttime Sleep in Drosophila melanogaster. Frontiers in neurology, 3, 24. PMID: 22375135.

Inagaki, H. K., Ben-Tabou de-Leon, S., Wong, A. M., Jagadish, S., Ishimoto, H., Barnea, G., Kitamoto, T., Axel, R. & Anderson, D. J. (2012). Visualizing neuromodulation in vivo: TANGO-mapping of dopamine signaling reveals appetite control of sugar sensing. Cell, 148(3), 583-95. PMID: 22304923.

Lark, A., Petruccelli, E. & Kitamoto, T. (2012). Drosophila DopEcR, a putative dual receptor for ecdysteroids and dopamine, is involved in behavioral response to food deprivation. Society for Neuroscience – Abstracts.

Chen, H., Kaas, G., Kasuya, J. & Kitamoto, T. (2011). A forward genetic screen for genes potentially involved in lithium responsive neurological processes using the fruit fly Drosophila melanogaster.

Lark, A., Ishimoto, H. & Kitamoto, T. (2011). A G-protein coupled receptor for the molting steroid hormone ecdysone plays important roles in regulation of courtship memory and circadian activity in Drosophila.

Soshnev, A. A., Ishimoto, H., McAllister, B. F., Li, X., Wehling, M. D., Kitamoto, T., Geyer, P. K. & Birchler, J. A. (2011). A conserved long noncoding RNA affects sleep behavior in Drosophila. Genetics, 189(2), 455-68. PMID: 21775470.

Ishimoto, H., Wang, Z., Wu, C. & Kitamoto, T. (2011). Involvement of cAMP signaling in regulation of neural and behavioral plasticity by a membrane-bound ecdysone receptor, DopEcR.

Ishimoto, H., Kitamoto, T. (2011). Beyond molting--roles of the steroid molting hormone ecdysone in regulation of memory and sleep in adult Drosophila. Fly, 5(3), 215-20. PMID: 21444997.

Ishimoto, H., Wang, Z., Wu, C. & Kitamoto, T. (2011). Non-genomic actions of the molting steroid hormone ecdysone on behavioral plasticity in adult Drosophila.

Ishimoto, H., Wang, Z., Wu, C. & Kitamoto, T. (2011). Non-genomic actions of the steroid hormone ecdysone on behavioral plasticity in adult Drosophila.

Iyengar, A., Ueda, A., Xing, X., Kitamoto, T. & Wu, C. H. (2011). A spectrum of neuronal excitability and central pattern generation phenotypes in distinct alleles of Drosophila Nav channel mutants. Society for Neuroscience – Abstracts.

Aldrich, B. T., Petruccelli, E. K., Kasuya, J. & Kitamoto, T. (2010). Reduced melanization response to UV-induced injury in the Drosophila amnesiac mutant.

Aso, Y., Siwanowicz, I., Bräcker, L., Ito, K., Kitamoto, T. & Tanimoto, H. (2010). Specific dopaminergic neurons for the formation of labile aversive memory. Current biology, 20(16), 1445-51. PMID: 20637624.

Ishimoto, H., Kitamoto, T. (2010). The steroid molting hormone Ecdysone regulates sleep in adult Drosophila melanogaster. Genetics, 185(1), 269-81. PMID: 20215472.

Soshnev, A. A., Ishimoto, H., McAllister, B., Li, X., Wehling, M. D., Kitamoto, T. & Geyer, P. K. (2010). Along non-coding RNA gene in the regulation of sleep homeostasis in Drosophila.

Ishimoto, H., Wang, Z., Wu, C. & Kitamoto, T. (2010). Non-genomic actions of the steroid hormone ecdysone in adult Drosophila.

Aldrich, B. T., Kasuya, J., Faron, M., Ishimoto, H. & Kitamoto, T. (2010). The amnesiac gene is involved in the regulation of thermal nociception in Drosophila melanogaster. Journal of neurogenetics, 24(1), 33-41. PMID: 19995327.

Kitamoto, T., Ishimoto, H., Olsen, D., Root, C. M. & Wang, J. W. (2010). Neural development, sensory processing, and behavioral control: highlight of Cold Spring Harbor Meeting on Neurobiology of Drosophila, September 29 to October 3, 2009. Journal of Neurogenetics, 24(1), 1-4. DOI: 10.3109/01677060903552676.

Kaas, A. G., Jewell, R., Kasuya, J., Ishimoto, H. & Kitamoto, T. (2010). Shudderer, a dominant mutation in Drosophila causing lithium-responsive neurological defects, is allelic to the voltage-gated sodium channel gene paralytic. Society Neuroscience – Abstracts.

Aldrich, B. T., Frakes, E. P., Kasuya, J., Hammond, D. L. & Kitamoto, T. (2009). Changes in expression of sensory organ-specific microRNAs in rat dorsal root ganglia in association with mechanical hypersensitivity induced by spinal nerve ligation. Neuroscience, 164(2), 711-23. PMID: 19699278.

Kasuya, J., Kaas, G. A. & Kitamoto, T. (2009). A putative amino acid transporter of the solute carrier 6 family is upregulated by lithium and is required for resistance to lithium toxicity in Drosophila. Neuroscience, 163(3), 825-37. PMID: 19619614.

Ishimoto, H., Wang, Z., Wu, C. & Kitamoto, T. (2009). Neuronal and behavioral plasticity regulated by non-genomic actions of the steroid hormone ecdysone in adult Drosophila.

Kasuya, J., Kaas, A. G. & Kitamoto, T. (2009). A putative amino acid transporter of the solute carrier 6 (SLC6) family is up-regulated by lithium and is required for resistance to lithium toxicity in Drosophila.

Kasuya, J., Ishimoto, H. & Kitamoto, T. (2009). Neuronal mechanisms of learning and memory revealed by spatial and temporal suppression of neurotransmission using shibire, a temperature-sensitive dynamin mutant gene in Drosophila melanogaster. Frontiers in molecular neuroscience, 2, 11. PMID: 19738923.

Kasuya, J., Kaas, G. & Kitamoto, T. (2009). Effects of lithium chloride on the gene expression profiles in Drosophila heads. Neuroscience research, 64(4), 413-20. PMID: 19410610.

Sakai, T., Kasuya, J., Kitamoto, T. & Aigaki, T. (2009). The Drosophila TRPA channel, Painless, regulates sexual receptivity in virgin females. Genes, brain, and behavior, 8(5), 546-57. PMID: 19531155.

Ishimoto, H., Kitamoto, T. (2009). The molting hormone ecdysone regulates courtship memory in Drosophila.

Ishimoto, H., Sakai, T. & Kitamoto, T. (2009). Ecdysone signaling regulates the formation of long-term courtship memory in adult Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of America, 106(15), 6381-6. PMID: 19342482.

Ishimoto, H., Kitamoto, T. (2009). Roles of the steroid molting hormone ecdysone in the formation of long-term courtship memory and modulation of sleep in adult Drosophila.

Kasuya, J., Kaas, G. A. & Kitamoto, T. (2009). The lithium-inducible solute carrier 6 transporters plays a critical role in resistance to lithium toxicity in Drosophila.

Kaas, A. G., Kasuya, J. & Kitamoto, T. (2009). Possible involvement of the calcineurin A subunit gene, CanA-14F, in the neurological phenotype of the lithium-responsive Drosophila mutant, Shudderer. Society Neuroscience – Abstracts.

Sakai, T., Kitamoto, T. (2008). Targeted expression of the temperature-sensitive dynamin to study neural mechanisms of complex behavior in Drosophila.

Ishimoto, H., Sakai, T. & Kitamoto, T. (2008). The molting hormone ecdysone regulates courtship memory in Drosophila. Learning and Memory: A Synthesis of Flies and Honeybees.

Sitaraman, D., Zars, M., LaFerriere, H., Chen, Y., Sable-Smith, A., Kitamoto, T., Rottinghaus, G. E. & Zars, T. (2008). Serotonin is necessary for place memory in Drosophila. Proceedings of the National Academy of Sciences of the United States of America, 105(14), 5579-84. PMID: 18385379.

Eberl, D. F., Kitamoto, T., Berke, B., O'Connor-Giles, K., Ueda, A., Lee, J., Ruan, H., Engel, J. E. & Ganetzky, B. (2008). Meeting report: genes, neurons, circuits, and behaviors: highlights of Cold Spring Harbor meeting on Drosophila neurobiology, October 3-7, 2007. Journal of neurogenetics, 22(1), 3-13. PMID: 18363161.

Ishimoto, H., Sakai, T. & Kitamoto, T. (2008). The role of insect steroid hormone ecdysone in courtship memory in Drosophila. Society Neuroscience – Abstracts.

Kaas, G. A., Ishimoto, H., Kasuya, J. & Kitamoto, T. (2007). Phenotypic analysis of lithium-responsive neurological mutant, Shudderer.

Ishimoto, H., Sakai, T. & Kitamoto, T. (2007). Roles of ecdysone signaling in regulation of sleep and courtship memory in Drosophila.

Aldrich, B. T., Frakes, E. P., Hammond, D. L. & Kitamoto, T. (2007). Changes in expression of sensory tissue-specific microRNAs in dorsal root ganglia following spinal nerve ligation.

Kitamoto, T., Kaas, G., Ishimoto, H. & Kasuya, J. (2007). Calcineurin and innate immune response genes are upregulated in Shudderer, the lithium responsive neurological mutant in Drosophila. Society Neuroscience – Abstracts.

Kitamoto, T., Kaas, G., Ishimoto, H. & Kasuya, J. (2007). Study of lithium-responsive neurological processes using the Drosophila mutant Shudderer.

Sakai, T., Kitamoto, T. (2006). Clock, love and memory: Circadian and non-circadian regulation of Drosophila mating behavior by clock genes. Sleep and Biological Rhythms, 4(3), 255-62. DOI: 10.1111/j.1479-8425.2006.00224.x.

Sakai, T., Kitamoto, T. (2006). Differential roles of two major brain structures, mushroom bodies and central complex, for Drosophila male courtship behavior. Journal of neurobiology, 66(8), 821-34. PMID: 16673386.

Sakai, T., Ishimoto, H. & Kitamoto, T. (2006). Involvement of the Drosophila transient potential ion channel gene painless in long-term courtship memory.

Ishimoto, H., Kitamoto, T. (2006). Dopaminergic and peptidergic systems centrally modulate behavioral response to noxious heat in Drosophila.

Sakai, T., Kitamoto, T. (2006). [Recent development of research on long-term memory in Drosophila]. Seikagaku. The Journal of Japanese Biochemical Society, 78(1), 38-41. PMID: 16491911.

Kitamoto, T., Ishimoto, H. & Sakai, T. (2006). Involvement of the Drosophila transient receptor potential ion channel gene painless in long-term courtship memory in Drosophila. pp. 290.18/OO68. Society Neuroscience – Abstracts.

Kaas, G. A., Ishimoto, H., Kasuya, J. & k, T. (2006). Shudderer: a Drosophila model to study the molecular mechanisms of lithium action. pp. 813.18/T19. Society Neuroscience – Abstracts.

Sakai, T., Kitamoto, T. (2005). The central complex plays a role in formation and retrieval of long-term courtship memory.

Sakai, T., Kidokoro, Y. & Kitamoto, T. (2005). Overexpression of a circadian clock gene, period, in Drosophila central complex enhances long-term memory formation.

Sakai, T., Kitamoto, T. & Kidokoro, Y. (2005). The clock gene, period, is essential for long-term memory formation in Drosophila. Cell Technology, 24, 50-1.

Flintoft, L. (2005). Beyond the body clock. (Vols. 6). pp. 3. London, England: Nature Reviews Genetics.

Sakai, T., Tamura, T., Kitamoto, T. & Kidokoro, Y. (2004). A clock gene, period, plays a key role in long-term memory formation in Drosophila. Proceedings of the National Academy of Sciences of the United States of America, 101(45), 16058-63. PMID: 15522971.

Sakai, T., Kitamoto, T. & Kidokoro, Y. (2004). Disruption of synaptic transmission through period-expressing cells block retrieval of long-term memory in Drosophila.

Broughton, S. J., Kitamoto, T. & Greenspan, R. J. (2004). Excitatory and inhibitory switches for courtship in the brain of Drosophila melanogaster. Current biology: CB, 14(7), 538-47. PMID: 15062094.

Sreenivasan, A. (2004). Fruit fly courtship puts neurons to work. Science.

Wang, Y., Chiang, A., Xia, S., Kitamoto, T., Tully, T. & Zhong, Y. (2003). Blockade of neurotransmission in Drosophila mushroom bodies impairs odor attraction, but not repulsion. Current biology, 13(21), 1900-4. PMID: 14588247.

Murphey, R. K., Froggett, S. J., Caruccio, P., Shan-Crofts, X., Kitamoto, T. & Godenschwege, T. A. (2003). Targeted expression of shibire ts and semaphorin 1a reveals critical periods for synapse formation in the giant fiber of Drosophila. Development, 130(16), 3671-82. PMID: 12835384.

O'Hare, K., Slack, C., Cheshire, A. & Kitamoto, T. (2003). Drosophila cleavage polyadenylation specificity factor (CPSF) mutants.

Kitamoto, T. (2002). Conditional disruption of synaptic transmission induces male-male courtship behavior in Drosophila. Proceedings of the National Academy of Sciences of the United States of America, 99(20), 13232-7. PMID: 12239352.

Kitamoto, T. (2002). Targeted expression of temperature-sensitive dynamin to study neural mechanisms of complex behavior in Drosophila. Journal of neurogenetics, 16(4), 205-28. PMID: 12745632.

Hall, C. T. (2002). Study finds chemical link to sex attraction. San Francisco Chronicle.

Surridge, C. (2002). Turning up the heat turns mutant male flies on to each other. Nature News.

Beckman, M. (2002). How to make a fly bi. Science Now.

Kitamoto, T. (2002). Analysis of neuronal functions using a temperature-sensitive molecular switch for synaptic transmission. Biophysics, 42, 236-41.

Choi, C. (2002). Gay flies. (Vols. 287). pp. 34. Scientific American.

Westphal, S. P. (2002). Turning up the heat makes flies fancy the same sex. (Vols. 175). pp. 15. New Scientist.

Kitamoto, T. (2001). Conditional disruption of synaptic transmission induces male-male courtship behavior in Drosophila.

Salvaterra, P. M., Kitamoto, T. (2001). Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP. Brain research. Gene expression patterns, 1(1), 73-82. PMID: 15018821.

Menzel, R., Müller, U. (2001). Neurobiology. Learning from a fly's memory. (Vols. 411). (6836), pp. 433-4. Nature. PMID: 11373661.

Dubnau, J., Grady, L., Kitamoto, T. & Tully, T. (2001). Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory. Nature, 411(6836), 476-80. PMID: 11373680.

Kitamoto, T. (2001). Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons. Journal of neurobiology, 47(2), 81-92. PMID: 11291099.

Waddell, S., Quinn, W. G. (2001). Learning how a fruit fly forgets. (Vols. 293). pp. 1271-2. Science.

Dubnau, J., Kitamoto, T. & Tully, T. (2001). Reversible disruption of synaptic transmission in Drosophila mushroom bodies blocks retrieval, but not acquisition or storage of olfactory memory. (Vols. 78). (Suppl. 1), pp. 12. The Internal Society for Neurochemistry, August 26-31, Journal of Neurochemistry.

Lee, D., O'Dowd, D. K. & Kitamoto, T. (2001). The vesicular ACh transporter regulates fast excitatory cholinergic transmission in Drosophila neurons. pp. 34. The Neurobiology of Drosophila Meeting, Cold Spring Harbor Laboratory, October 3-7.

Wang, Y., Kitamoto, T. & Zhong, Y. (2001). Drosophila mushroom bodies in the perception of odor attractiveness. (Vols. 27). Society Neuroscience – Abstracts.

Waddell, S., Armstrong, J. D., Kitamoto, T., Kaiser, K. & Quinn, W. G. (2000). The amnesiac gene product is expressed in two neurons in the Drosophila brain that are critical for memory. Cell, 103(5), 805-13. PMID: 11114336.

Waddell, S., Armstrong, J. D., Kitamoto, T., Kaiser, K. & Quinn, W. G. (2000). The amnesiac gene product is strongly expressed in two neurons in the Drosophila brain that are critical for memory.

Salvaterra, P. M., Sun, B., Lee, M. & Kitamoto, T. (2000). Visualization of cholinergic neurons using Gal4 x UAS-GFP lines.

Kitamoto, T., Xie, X., Wu, C. F. & Salvaterra, P. M. (2000). Isolation and characterization of mutants for the vesicular acetylcholine transporter gene in Drosophila melanogaster. Journal of neurobiology, 42(2), 161-71. PMID: 10640324.

Bengtson, J., Kitamoto, T. (1999). Conditional modification of behavior by targeted expression of a temperature-sensitive shibire allele in subsets of neurons.

Wang, W., Kitamoto, T. & Salvaterra, P. M. (1999). Drosophila choline acetyltransferase temperature-sensitive mutants. Neurochemical research, 24(8), 1081-7. PMID: 10478949.

Kitamoto, T. (1999). The Drosophila cholinergic locus – its operon-like structure and gene regulation.

Kitamoto, T., Wang, W. & Salvaterra, P. M. (1998). Structure and organization of the Drosophila cholinergic locus. The Journal of biological chemistry, 273(5), 2706-13. PMID: 9446576.

Wang, W., Kitamoto, T. & Salvaterra, P. M. (1998). Heterologous expression and structure-function studies of choline acetyltransferase using temperature-sensitive mutants. (Vols. 24). pp. 1321. Society Neuroscience – Abstracts.

Kitamoto, T., Salvaterra, P. M. (1997). Structure and regulation of the Drosophila cholinergic locus-A eukaryotic “operon”.

Yasuyama, K., Kitamoto, T. & Salvaterra, P. M. (1997). Distribution of putative cholinergic sensory neurons associated with proprioceptive and taste sensilla in the legs of Drosophila melanogaster. Comparative Biochemistry and Physiology, 14, 289.

Yasuyama, K., Kitamoto, T. & Salvaterra, P. M. (1996). Differential regulation of choline acetyltransferase expression in adult Drosophila melanogaster brain. Journal of neurobiology, 30(2), 205-18. PMID: 8738750.

Yasuyama, K., Kitamoto, T. & Salvaterra, P. M. (1996). Distribution and central projections of putative cholinergic leg sensory neurons in Drosophila. Zoological science, 13(Suppl), 120.

Kitamoto, T., Wang, W., Sun, B. & Salvaterra, P. M. (1996). Structure and regulation of choline acetyltransferase and vesicular acetylcholine transporter genes in Drosophila. (Vols. 22). pp. 1032. Society Neuroscience – Abstracts.

Yasuyama, K., Kitamoto, T. & Salvaterra, P. M. (1996). Differential regulation of choline acetyltransferase expression in the central nervous system of Drosophila: a study using temperature-sensitive mutants. (Vols. 25). pp. 103. Neuroscience Research.

Yasuyama, K., Kitamoto, T. & Salvaterra, P. M. (1995). Localization of choline acetyltransferase-expressing neurons in the larval visual system of Drosophila melanogaster. Cell and tissue research, 282(2), 193-202. PMID: 8565051.

Yasuyama, K., Kitamoto, T. & Salvaterra, P. M. (1995). Immunocytochemical study of choline acetyltransferase in Drosophila melanogaster: an analysis of cis-regulatory regions controlling expression in the brain of cDNA-transformed flies. The journal of comparative neurology, 361(1), 25-37. PMID: 8550879.

Kitamoto, T., Salvaterra, P. M. (1995). Molecular genetic analyses of the Drosophila vesicular acetylcholine transporter gene.

Kitamoto, T., Ikeda, K. & Salvaterra, P. M. (1995). Regulation of choline acetyltransferase/lacZ fusion gene expression in putative cholinergic neurons of Drosophila melanogaster. Journal of neurobiology, 28(1), 70-81. PMID: 8586966.

Kitamoto, T., Salvaterra, P. M. (1995). A POU homeo domain protein related to dPOU-19/pdm-1 binds to the regulatory DNA necessary for vital expression of the Drosophila choline acetyltransferase gene. The Journal of neuroscience: the official journal of the Society for Neuroscience, 15(5 Pt 1), 3509-18. PMID: 7751926.

Kitamoto, T., Salv, P. M. & McCaman, R. E. (1994). A POU homeo domain protein related to dPOU-19/pdm-1 binds to the regulatory DNA necessary for vital expression of the Drosophila choline acetyltransferase gene. (Vols. 20). pp. 80. Society Neuroscience – Abstracts.

Liang, S., Kitamoto, T. & Salvaterra, P. M. (1994). An octamer-like motif may play an important role in ChAT gene regulation: cloning the ChAT gene regulatory region of D. virilis. (Vols. 20). pp. 80. Society Neuroscience – Abstracts.

Kitamoto, T., Salvaterra, P. M. (1993). An “essential” regulatory motif in the Drosophila choline acetyltransferase gene.

Kitamoto, T., Salvaterra, P. M. (1993). Developmental regulatory elements in the 5' flanking DNA of the Drosophila choline acetyltransferase gene. Roux's archives of developmental biology : the official organ of the EDBO, 202(3), 159-69. PMID: 28305993.

Salvaterra, P. M., Kitamoto, T. & Ikeda, K. (1993). Molecular genetic specification of cholinergic neurons. Progress in brain research, 98, 167-73. PMID: 8248505.

Yasuyama, K., Kitamoto, T. & Salvaterra, P. M. (1993). Choline acetyltransferase expression in Drosophila cDNA transformants. (Vols. 19). pp. 915. Society Neuroscience – Abstracts.

Miyagawa-Tomita, S., Kitamoto, T., Momma, K., Takao, A. & Momoi, T. (1992). Cellular retinoic acid binding protein type II was preferentially localized in medium and posterior parts of the progress zone of the chick limb bud. Biochemical and biophysical research communications, 185(1), 217-23. PMID: 1318041.

Kitamoto, T., Ikeda, K. & Salvaterra, P. M. (1992). Analysis of cis-regulatory elements in the 5' flanking region of the Drosophila melanogaster choline acetyltransferase gene. The journal of neuroscience, 12(5), 1628-39. PMID: 1374460.

Kitamoto, T., Ikeda, K. & Salvaterra, P. M. (1992). Distribution of cholinergic neurons in whole mount preparations of Drosophila. (Vols. 18). pp. 1504. Society Neuroscience – Abstracts.

Andrisani, V., Kitamoto, T. & Sa, P. M. (1992). Feedback regulation of choline acetyltransferase expression in Drosophila. (Vols. 18). pp. 1504. Society Neuroscience – Abstracts.

Kitamoto, T., Salvaterra, P. M. (1991). Regulation of the Drosophila choline acetyltransferase gene during development.

Ikeda, K., Kitamoto, T. & Salvaterra, P. M. (1991). Analysis of regulatory elements in the 5’ flanking region of the Drosophila choline acetyltransferase gene. (Vols. 17). pp. 1521. Society Neuroscience – Abstracts.

Kitamoto, T., Ikeda, K. & Salvaterra, P. M. (1990). cis-Regulatory elements of the Drosophila choline acetyltransferase gene. (Vols. 16). pp. 199. Society for Neuroscience - Abstracts.

Kitamoto, T., Momoi, M. & Momoi, T. (1989). Expression of cellular retinoic acid binding protein II (chick-CRABP II) in the chick embryo. Biochemical and biophysical research communications, 164(1), 531-6. PMID: 2553021.

Salvaterra, P. M., Andorisani, V., Kitamoto, T., Sugihara, H. (Eds.) (1989). Transcriptional and translational regulation of choline acetyltransferase.

Momoi, T., Kitamoto, T., Kasuya-Sato, J., Seno, H. & Momoi, M. (1989). Developmental changes in the expression and distribution of cellular retinoic acid binding-protein (CRABP) in the central nervous system of the chick embryo. Biomedical Research, 10(1), 43-8.

Aikawa, H., Momoi, T., Kitamoto, T., Arahata, K. & Momoi, M. (1989). Expression of cellular retinoic acid binding protein (CRABP) in adult rat brain. Biomedical Research, 10(3), 247-50. DOI: 10.2220/biomedres.10.247.

Kitamoto, T., Momoi, T. & Momoi, M. (1988). The presence of a novel cellular retinoic acid-binding protein in chick embryos: purification and partial characterization. Biochemical and biophysical research communications, 157(3), 1302-8. PMID: 2849937.

Momoi, T., Kitamoto, T., Seno, H. & Momoi, M. (1988). The distribution of cellular retinoic acid binding protein (CRABP) in the central nervous system of the chick embryo during the development. Proceedings of the Japan Academy, Series B, 64(9), 294-7. DOI: 10.2183/pjab.64.294.

Kitamoto, T., Nishigai, M., Sasaki, T. & Ikai, A. (1988). Structure of fatty acid synthetase from the Harderian gland of guinea pig. Proteolytic dissection and electron microscopic studies. Journal of molecular biology, 203(1), 183-95. PMID: 3184185.

Kitamoto, T., Nishigai, M., Ikai, A., Ohashi, K. & Seyama, Y. (1985). The quaternary structure and activity of newly purified fatty acid synthetase from the Harderian gland of guinea-pig. Biochimica et biophysica acta, 827(2), 164-73. PMID: 3967036.

Ikai, A., Kitamoto, T. & Nishigai, M. (1983). Alpha-2-macroglobulin-like protease inhibitor from the egg white of cuban crocodile (Crocodylus rhombifer). Journal of biochemistry, 93(1), 121-7. PMID: 6188750.

Kitamoto, T., Nakashima, M. & Ikai, A. (1982). Hen egg white ovomacroglobulin has a protease inhibitory activity. Journal of biochemistry, 92(5), 1679-82. PMID: 6185473.