Instrumentation

Canto II

PDF iconCanto II Configuration.pdfCanto II Configuration.pdf

TWO Excitation Sources:

Blue Laser 488 nm
Mirror Band Pass Filter (example dye detected)
735 LP 780/60 (PE-Cy7)
655 LP 670LP (PerCP-Cy5.5, PerCP)
610LP  
556 LP 585/42 (PE)
502 LP 530/30 (FITC)
  488/10 (side scatter)

 

Red Laser 633 nm
Mirror Band Pass Filter (example dye detected)
735 LP 780/60 (PE-Cy7)
685 LP  
  660/20 (APC, Alexa Fluor 647)

 

LSR II

PDF iconLSR II Configuration.pdfLSR II Configuration.pdf

4 Excitation Sources:

Violet Laser 405 nm
Mirror Band Pass Filter (example dye detected)
780 LP 780/60 (Brilliant Violet 786)
690 LP 710/50 (Brilliant Violet 711)
630 LP 660/20 (Brilliant Violet 650)
595 LP 610/20 (Brilliant Violet 605)
505 LP 525/50 (Brilliant Violet 510, Violet 500)
  450/50 (Pacific Blue, Brilliant Violet 421, Violet 450)

 

Blue Laser 488 nm
Mirror Band Pass Filter (example dye detected)
556 LP 695/40 (PerCP, PerCP-Cy5.5, PE-Blue)
505 LP 515/20 (FITC, GFP, CFSE)
  488/10 (side scatter)

 

Green 532 nm
Mirror Band Pass Filter (example dye detected)
735 LP 780/60 (PE-Cy7)
675 LP 685/35 (PE-Cy5.5)
635 LP 660/20 (PE-Cy5)
600 LP 610/20 (PE-Texas Red, 7-AAD)
  576/26 (PE-Green)

 

Red Laser 633 nm
Mirror Band Pass Filter (example dye detected)
755 LP 780/60 (APC-Cy7)
685 LP 730/45(Alexa Fluor 700)
  660/20 (APC, Alexa Fluor 647)